lecture 09 ch 7&9 allosteric hemoglobin opst-2
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7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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<- $*" R&"2"-#" 1=
,0012$"&3# ,#$3[,$1&̂"-U;6" *,2 *37*,==3-3$; =1& 2%82$&,$"
7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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H3#*,"032IH"-$"- <-*383$31-
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7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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<- ."1:;*"6170183-^ E" *,2 1-0; T
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R123$31-^ $*" *"6170183-
610"#%0" #*,-7"2 3-
h%,$"&-,&; 2$&%#$%&"
7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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G+K, =, H-)> 73&XX
#B),* 3?79,. '-./( )>, -*3. -. )>, >,=, *-.9 3B
>,=3983'-.Y
UV G>, -*3. +)3= ',03=,( 8+*9,*
WV S-( Z[ +.0>3*( )>, -*3. +)3= 3&)(-/, )>,
63*6>7*-. *-.9
\V G>, >,=, 9*3&6 /-((30-+),(]V I3.-0 '3./( -. )>, +86>+Y',)+ /-=,* -.),*B+0,
+*, /-(*&6),/
7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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@ ,-. C B$,$"2 1= 5"6170183-
C n C"-2" 2$,$" O."1:;*"6170183-S
i ?M 83-.3-7 $&377"&2 , C @ #1-=1&6,$31-,0 #*,-7"
@ n @"0,:". 2$,$"^ *37*"& ,==3-3$; =1& ?M $*,- $*" C 2$,$"
)1-=1&6,$31-,0 #*,-7" =&16 $*" C 2$,$" $1 $*" @ 2$,$"
3-[10["2 .32&%R$3-7 31- R,3&2 8"$X""- $*" !" .36"&
3-$"&=,#" i !1X ?M 2$,$"^ 31-3# 3-$"&,#$31- ,$ 3-$"&=,#"
i DR1- ?M 83-.3-7^ 3&1- 2*3=$2 R123$31-^ 61["2 ,221#3,$". 532 ,-.
.32&%R$2 $*" 3-$"&=,#"
_ C =1&6g 2,0$ 8&3.7" 3-$,#$
_ @ =1&6g 2,0$ 8&3.7" .32&%R$".
7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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@"#,00g K36"&2 *"0.
$17"$*"& 8; X",W 31-3#
,-. *;.&17"- 81-.2
<- b$,%$c C 2$,$"^ k
61&" 2,0$ 8&3.7"2
8"$X""- 2%8%-3$2 $*,-
b&"0,:".c @ =1&6
7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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s%,$"&-,&; 5"6170183- ":*383$2
#11R"&,$3[3$; X3$* 3-#&",23-7 1:;7"-
,[,30,8303$;
7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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C*" 1:;7"- .3221#3,$31- #%&[" =1&
6;170183- *,2 , *;R"&8103# 2*,R" $*,$&"=0"#$2 $*" =,#$ $*,$g
\V H;170183- &"["&2380; 83-.2 , 23-70"
610"#%0" 1= 1:;7"-
MV H;170183-c2 Q 2%8%-3$2 #11R"&,$" 3-83-.3-7 1:;7"-
PV C*" ."1:; =1&6 1= 6;170183- 32 3- $*" @I
2$,$"
QV H;170183- *,2 , 01X"& 1:;7"- ,==3-3$;$*,- *"6170183-
TV H;170183- *,2 *"6" ,-. *"6170183-.1"2 -1$
7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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_ )16R,&" ,-. #1-$&,2$ 1:;7"- 83-.3-7 3- 6;170183- [2 *"6170183-
_ K32#%22 $*" 610"#%0,& 8,232 =1& #11R"&,$3[3$; 3- 1:;7"- 83-.3-7 $1
*"6170183-
_ 9:R01&" ,0012$"&3# &"7%0,$1&2 1= *"6170183-
_ J"#16" =,6303,& X3$* 23#W0" #"00 ,-"63, ,-. )? "=="#$2 1- *"6170183-
!",&-3-7 ?8j"#$3["2
7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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5"6170183- ,-. ?:;7"- C&,-2R1&$
<- 1&."& $1 83-. 1:;7"- 3- $*" 0%-72 ,-. &"0",2" 1:;7"- 3-$322%"2tV
5"6170183-c2 ,==3-3$; =1& 1:;7"- 32 &"7%0,$". 8; ,$ 0",2$ Q
=,#$1&2 i ?M 0"["02 O)11R"&,$3[3$;S
i ,-. /0012$"&3# @"7%0,$1&2
_ )?M 0"["02
_ R5_ M^PIJ4o
7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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5"6170183- ,-. )?M 9:R1&$
)?M 32 R&1.%#". 8; 6"$,810326 3- $322%"2 ,-. 6%2$ 8" ":R1&$".
i K3==%2"2 $1 @J)2^ =1&62 #,&81-3# ,#3.!83#,&81-,$"
i /021^ ":R1&$". 3- $*" =1&6 1= , #,&8,6,$" 1- FI$"&63-3 1=
*"6170183- 2%8%-3$2
i )1-="&2 -"7,$3[" #*,&7" 1- $"&63-,0 ,63-1 7&1%R2^ X*3#* 03" 3- lq
3-$"&=,#"
_ F"7,$3[" #*,&7"II=,[1&2 2,0$ 8&3.7" =1&6,$31-
)?M 81%-. $1 *"6170183- 2$,8303U"2 C =1&6 i @"0",2" ?M
58IF5M m )?M 58IF5I)??I m 5m
7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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R5 9=="#$ 1- ?M J3-.3-7 $1 5"6170183-
J011. 3- 0%-72 R5 u 8011. 3- #,R300,&3"2 1= 6"$,8103#,00; ,#$3[" $322%"2
58 ,==3-3$; =1& 1:;7"- ."R"-."-$ %R1- R5
i ?:;7"- &"0",2". ,$ 01X"& R5vC 2$,$" =,[1&".
_ <1-3# 81-.2 .%" $1 R&1$1-,$". 1= 532 &"23.%"2 ,$ .36"& 3-$"&=,#"
i ?:;7"- 2"h%"2$"&". ,$ *37*"& R5 i@ 2$,$" =,[1&".
s%"2$31-2g
<- 0%-72g 537*"& R5^ 537* 1:;7"-vX*3#* =1&6 R&".163-,$"2e
<- 6"$,8103#,00; ,#$3[" $322%"2g !1X"& R5^ !1X 1:;7"-vd*3#* =1&6
R&".163-,$"2e
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R5 9=="#$ 1- ?M J3-.3-7 $1 5"6170183-
<- $322%"2^ 1:;*"6170183- 3- @I2$,$"
O."R&1$1-,$". 23." #*,3-2S ,##"R$2 R&1$1-2
&"0",2". .%&3-7 6"$,810326 O3V"V 0,#$3# ,#3. 1&
#,&81-3# ,#3.S
@! C #1-["&231- =,[1&2 &"0",2" 1= 1:;7"-
<- 0%-72^ ."1:;*"6170183- 3- C 2$,$" &"0",2"2
R&1$1-2 3- *37*"& R5 "-[3&1-6"-$ O.%" $1
":*,0,$31- 1= )?MS
C! @ #1-["&231- =,[1&2 83-.3-7 1:;7"-
7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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I)?M 32 $&,-2R1&$". $1 0%-72 1- 58 ,-. ,2 83#,&81-,$"
I5m 31-2^ X*3#* #1-$&38%$" $1 -%0? )CC)A&D ,&" 7"-"&,$". .%&3-7
83#,&81-,$" =1&6,$31-
v83-. 532 &"23.%"2 $1 =,[1& C 2$,$"I1:;7"- &"0",2"
7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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<- $*" 0%-72^ &".%#". )?M #1-#"-$&,$31- .%" $1 ":*,0,$31-
I=,[1&2 83#,&81-,$" m 5m ! )?M ,-. 5M?
I3-#&",23-7 R5 0",.2 $1 ."R&1$1-,$31- 1= 532 &"23.%"2v@ 2$,$" =,[1&".
7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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J<o 4<)CD@9g
_ 537* w)?Mx ,-. w5mx 2$,8303U" C =1&6 1= 58 3- $322%"2
_ !1X w)?Mx ,-. w5mx 0",. $1 C!@ #1-["&231- 3- 0%-72
.#""&/% #0,1&+ 23&'& 4#$% !5*+6!+%
7&"89&' #0,1&+ 23&'& 4#$% +&&6&6
@"7%0,$31- 1= ? J3-.3-7 8;
7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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@"7%0,$31- 1= ?M J3-.3-7 8;
M^PIJ32R*12R*170;#"&,$" OM^PIJ4oS
M^PIJ4o ."#&",2"2 *"6170183-c2 1:;7"- ,==3-3$;
i J3-.2 ."1:;*"6170183- ,-. 2$,8303U"2 C 2$,$"
i !"["02 &"7%0,$". $1 ,..&"22 *;R1:3# 23$%,$31-2
E"$,0 *"6170183- 0,#W2 M^PIJ4o 83-.3-7 23$"I6,$"&-,0 ! ="$,0 1:;7"- $&,-2="&
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d*3#* =,#$1&2 2"&[" $1 &"0",2" 1:;7"- 3-
$322%"2g
\V !1X )?M
MV 90"[,$". w5mx
PV !1X M^P J4o
7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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_ )16R,&" ,-. #1-$&,2$ 1:;7"- 83-.3-7 3- 6;170183- [2 *"6170183-
_ K32#%22 $*" 610"#%0,& 8,232 =1& #11R"&,$3[3$; 3- 1:;7"- 83-.3-7 $1
*"6170183-
_ 9:R01&" ,0012$"&3# &"7%0,$1&2 1= *"6170183-
_ J"#16" =,6303,& X3$* 23#W0" #"00 ,-"63, ,-. )? "=="#$2 1- *"6170183-
!",&-3-7 ?8j"#$3["2
7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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o"-"$3# 6%$,$31- 3- *"6170183- 7"-"
I1-" -1-#1-2"&[,$3[" ,63-1 ,#3. 2%82$3$%$31-I,77&"7,$"2 %-."& *;R1:3# #1-.3$31-2
B3#W0" #"00 ,-"63,
7/24/2019 Lecture 09 Ch 7&9 Allosteric Hemoglobin Opst-2
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),&81- H1-1:3." 41321-3-7
)? 83-.2 $37*$0; $1 *"6170183- 3&1-
J3-.3-7 1= )? 1- 1-" *"6" 23$" -"7,$3["0; ,=="#$2 1:;7"-
83-.3-7 1- &"6,3-3-7 23$"2