lecture 1 cell
TRANSCRIPT
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MC
AT
Prep
Exam
PowerPoint® Lecture Slides are prepared by Dr. Isaac Barjis, Biology Instructor 1
Cell Structure and Function
Lecture 1
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Outline
Cellular Level of Organization Cell theory Cell size
Prokaryotic Cells Eukaryotic Cells
Organelles Nucleus and Ribosome Endomembrane System Other Vesicles and Vacuoles Energy related organelles Cytoskeleton
Centrioles, Cilia, and Flagella
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Cell Theory
Cell was not discovered untill the development of Microscope
Detailed study of the cell began in the 1830s A unifying concept in biology States that:
All organisms are composed of cells All cells come only from preexisting cells Cells are the smallest structural and functional unit of
organisms Cells carry genetic information in the form of DNA
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Sizes of Living Things
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Cell Size
Cells range in size from one millimeter down to one micrometer
Cells need a large surface area of plasma membrane to adequately exchange materials.
The surface‑area‑to‑volume ratio requires that cells be small
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Surface to Volume Ratio
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Microscopy Today: Compound Light Microscope
Light passed through specimen
Focused by glass lenses
Max magnification about 1000X
Resolves objects separated by 0.2 m, 500X better than human eye
Resolution is limited by the wavelength of light (nanometer)
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Compound Light Microscope
Diaphragm – controls amount of light – important for image contrast
Coarse Adjustment Knob – focuses the image
Fine Adjustment Knob – finely focuses the image
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Microscopy Today: Transmission Electron Microscope
Abbreviated T.E.M.
Uses a beam of electrons to allow 100 fold higher magnification Because it uses beam of electrons, its resolution is at
the atomic level (picometer)
Tissue must be fixed and sectioned
Can living specimen be examined by T.E.M?
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Transmission Electron Microscope
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Microscopy Today: Immunofluorescence Light Microscope
Antibodies developed against a specific protein Fluorescent dye molecule attached to antibody
molecules Specimen exposed to fluorescent antibodies
Ultra-violet light (black light) passed through specimen Fluorescent dye glows in color where antigen is
located Emitted light is focused by glass lenses onto human
retina Allows mapping distribution of a specific protein
in cell
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Microscopy and Amoeba proteus
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Cells Under the Microscope
phase-contrast light microscope - look at unstained living animal cells.
electron microscope - look at organelles e.g. ribosomes.
fluorescence microscope - look at a living cell expressing green fluorescent protein or to do confocal microscopy.
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Autoradiography
Radioactive compounds decay or transform into other compounds or elements.
An autoradiograph is an image on an x-ray film or nuclear emulsion produced by the pattern of decay emissions (e.g., beta particles or gamma rays) from a distribution of a radioactive substance
Autoradiography can also uses radioactive molecule to study biochemical activity, Protein synthesis
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Cell Fractionation and Differential Centrifugation
Cell fractionation is the breaking apart of cellular components
Differential centrifugation:
Allows separation of cell parts
Separated out by size & density
Works like spin cycle of washer
The faster the machine spins, the smaller the parts that are settled out
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Cell Fractionation and Differential Centrifugation
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Eukaryotes Vs Prokaryotes
Eukaryotic Cells Prokaryotic Cells
The cells of “complex” organisms, including all plants, Protists, fungi and animals
“Simple” organisms, including bacteria and cyanobacteria (blue-green algae)
Contain a nucleus and membrane bound organelles
Lack a nucleus and other membrane-encased organelles.
Can specialize for certain functions, such as absorbing nutrients from food or transmitting nerve impulses; multicellular organs and organisms
Usually exist as single, virtually identical cells
Cell Wall present in Plants and Fungi onlyRibosome: 40s, 60S
Cell Wall
Ribosome: 30S, 50S
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The Structure of Bacteria
Occur in three basic shapes: Spherical coccus, Rod-shaped bacillus, Spiral spirillum (if rigid) or spirochete (if flexible).
Cell Envelope includes: Plasma membrane - lipid bilayer with imbedded and peripheral
protein Cell wall - maintains the shape of the cell
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The Structure of Bacteria
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The Structure of Bacteria
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The Structure of Bacteria Cytoplasm & Appendages
Cytoplasm Semifluid solution
Bounded by plasma membrane Contains water, inorganic and organic molecules, and enzymes.
Nucleoid is a region that contains the single, circular DNA molecule.
Plasmids are small accessory (extrachromosomal) rings of DNA
Appendages Flagella – Provide motility Fimbriae – small, bristle-like fibers that sprout from the cell
surface Sex pili – rigid tubular structures used to pass DNA from cell to
cell
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Eukaryotic Cells
Domain Eukarya includes: Protists
Fungi
Plants
Animals
Cells contain: Membrane-bound nucleus that houses DNA
Specialized organelles
Plasma membrane
Much larger than prokaryotic cells
Some cells (e.g., plant cells) have a cell wall
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Hypothesized Origin of Eukaryotic Cells
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Eukaryotic Cells: Organelles
Eukaryotic cells are compartmentalized They contain small structures called organelles
Perform specific functions Isolates reactions from others
Two classes of organelles: Endomembrane system:
Organelles that communicate with one another Via membrane channels Via small vesicles
Energy related organelles Mitochondria & chloroplasts Basically independent & self-sufficient
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Plasma Membrane
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Animal Cell Anatomy
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Plant Cell Anatomy
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Cytosole
Cytosol, contains many long, fine filaments of protein that are responsible for cell shape and structure and thereby form the cell’s cytoskeleton
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Nucleus
Command center of cell, usually near center Separated from cytoplasm by nuclear envelope
Consists of double layer of membrane Nuclear pores permit exchange between nucleoplasm
& cytoplasm Contains chromatin in semifluid nucleoplasm
Chromatin contains DNA of genes, and proteins (Histones)
Condenses to form chromosomes Chromosomes are formed during cell division
Nucleolus is a dense structure in the nucleus Synthesize ribosome RNA (rRNA)
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Anatomy of the Nucleus
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Ribosomes
Are the site of protein synthesis in the cell
Composed of rRNA and protein Consists of a large subunit and a small subunit
Subunits made in nucleolus
May be located: On the endoplasmic reticulum (thereby making it
“rough”), or
Free in the cytoplasm
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Nucleus, Ribosomes, & ER
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Endomembrane System
Series of intracellular membranes that compartmentalize the cell
Restrict enzymatic reactions to specific compartments within cell
Consists of: Nuclear envelope Membranes of endoplasmic reticulum Golgi apparatus Vesicles
Several types Transport materials between organelles of system
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Endomembrane System:The Endoplasmic Reticulum
A system of membrane channels and saccules (flattened vesicles) continuous with the outer membrane of the nuclear envelope
Rough ER Studded with ribosomes on cytoplasmic side Protein anabolism
Synthesizes proteins Modifies and processes proteins
Adds sugar to protein Results in glycoproteins
Smooth ER No ribosomes Synthesis of lipids Site of various synthetic processes, detoxification, and storage Forms transport vesicles
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Endoplasmic Reticulum
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Endomembrane System:The Golgi Apparatus
Golgi ApparatusConsists of flattened, curved saccules
Resembles stack of hollow pancakes
Modifies proteins and lipidsReceives vesicles from ER on cis (or inner face)
Modifies them and repackages them in vesicles
Release the vesicles from trans (or outer face) Within cell
Export from cell (secretion, exocytosis)
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Golgi Apparatus
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Endomembrane System: Lysosomes
Membrane-bound vesicles (not in plants) Produced by the Golgi apparatus Contain powerful digestive enzymes and are highly
acidic Digestion of large molecules Recycling of cellular debris and resources Autolysis may occur in injured or dying cell to cause
apoptosis (programmed cell death, like tadpole losing tail)
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Lysosomes
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Endomembrane System: Summary
Proteins produced in rough ER and lipids from smooth ER are carried in vesicles to the Golgi apparatus.
The Golgi apparatus modifies these products and then sorts and packages them into vesicles that go to various cell destinations.
Secretory vesicles carry products to the membrane where exocytosis produces secretions.
Lysosomes fuse with incoming vesicles and digest macromolecules.
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Endomembrane System: A Visual Summary
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Peroxisomes
Similar to lysosomes Membrane-bounded vesicles Enclose enzymes that rid the cell of toxic peroxides Participate in the metabolism of fatty acids and many
other metabolites
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Peroxisomes
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Vacuoles
Membranous sacs that are larger than vesicles Store materials that occur in excess Others very specialized (contractile vacuole)
Plants cells typically have a central vacuole Up to 90% volume of some cells Functions in:
Storage of water, nutrients, pigments, and waste products Development of turgor pressure Some functions performed by lysosomes in other eukaryotes
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Vacuoles
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Energy-Related Organelles:Chloroplast Structure
Bounded by double membrane
Inner membrane infolded
Forms disc-like thylakoids, which are stacked to form grana
Suspended in semi-fluid stroma
Chlorophyll
Green photosynthetic pigment
Chlorophyll capture solar energy
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Energy-Related Organelles: Chloroplasts
Serve as the site of photosynthesis
Captures light energy to drive cellular machinery
Photosynthesis
Synthesizes carbohydrates from CO2 & H2O
Makes own food using CO2 as only carbon source
Inorganic molecules (Energy-poor compounds) are converted to organic molecules (energy-rich compounds)
Only plants, algae, and certain bacteria are capable of conducting photosynthesis
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Chloroplast Structure
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Energy-Related Organelles: Mitochondria
Smaller than chloroplast
Contain ribosomes and their own DNA
Surrounded by a double membrane
Inner membrane surrounds the matrix and is convoluted (folds) to form cristae.
Matrix – Inner semifluid containing respiratory enzymes
Break down carbohydrates
Involved in cellular respiration
Produce most of ATP utilized by the cell
Contain their own DNA and ribosome i.e. they are semiautonomous
Inherited from Oocyte
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Mitochondrial Structure
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Mitochondrial Origin Hypothesis
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The Cytoskeleton Maintains cell shape
Assists in movement of cell and organelles
Aids movement of materials in and out of cells
Three types of macromolecular fibers
Microfilament
Intermediate Filaments
Microtubules
Assemble and disassemble as needed
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The Cytoskeleton: Actin Filaments
Microfilament are rods of actin Extremely thin filaments like twisted pearl
necklace Support for microvilli in intestinal cells Intracellular traffic control
For moving stuff around within cell Cytoplasmic streaming
Function in pseudopods of amoeboid cells Important component in muscle contraction
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The Cytoskeleton: Actin Filament Operation
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The Cytoskeleton: Intermediate Filaments
Intermediate in size between actin filaments and microtubules
Rope-like assembly of fibrous polypeptides
Functions: Support nuclear envelope
Cell-cell junctions, like those holding skin cells tightly together
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The Cytoskeleton: Microtubules
Hollow cylinders made of two globular proteins called and tubulin
Spontaneous pairing of and tubulin molecules form structures called dimers
Dimers then arrange themselves into tubular spirals of 13 dimers around
Assembly: Under control of Microtubule Organizing Center (MTOC) Most important MTOC is centrosome
Function: Provide framework for movement of organelle within cell Direct separation of chromosomes during cell division (e.g.
Centrioles are composed of microtubules) Provide locomotion and movement (e.g. flagella and cilia)
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The Cytoskeleton: Microtubule Operation
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The Cytoskeleton
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Microtubular Arrays: Centrioles
Short, hollow cylinders One pair per animal cell
Located in centrosome of animal cells Oriented at right angles to each other Separate during mitosis to determine plane of division
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Cytoskeleton: Centrioles
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Microtubular Arrays: Cilia and Flagella
Hair-like projections from cell surface that aid in cell movement
In eukaryotes, cilia are much shorter than flagella Cilia move in coordinated waves like oars Flagella move like a propeller or cork screw
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Structure of a Flagellum
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Comparison of Prokaryotic and Eukaryotic Cells
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MC
AT
Exam
Prep
PowerPoint® Lecture Slides are prepared by Dr. Isaac Barjis, Biology Instructor 64
Membrane Structure and Function
Lecture 1
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Outline
Membrane Models Fluid-Mosaic
Plasma Membrane Structure and Function Phospholipids Proteins
Plasma Membrane Permeability Diffusion Osmosis Transport Via Carrier Proteins
Cell Surface Modifications
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Structure and Function:The Phospholipid Bilayer
The plasma membrane is common to all cells Separates:
Internal living cytoplasmic from External environment of cell
Phospholipid bilayer: External surface lined with hydrophilic polar heads Cytoplasmic surface lined with hydrophilic polar heads Nonpolar, hydrophobic, fatty-acid tails sandwiched in
between
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Unit Membrane
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Membrane Models
Fluid-Mosaic ModelThree components:
Basic membrane referred to as phospholipid bilayer
Protein moleculesFloat around like icebergs on a seaMembrane proteins may be peripheral or integral
Peripheral proteins are found on the inner membrane surface
Integral proteins are partially or wholly embedded (transmembrane) in the membrane
Some have carbohydrate chains attachedCholesterol
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The Fluid Mosaic Model
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Transmembrane Proteins
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Functions of Membrane Proteins
Channel Proteins: Tubular Allow passage of molecules through membrane
Carrier Proteins: Combine with substance to be transported Assist passage of molecules through membrane
Cell Recognition Proteins: Provides unique chemical ID for cells Help body recognize foreign substances
Receptor Proteins: Binds with messenger molecule Causes cell to respond to message
Enzymatic Proteins: Carry out metabolic reactions directly
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Membrane Protein Diversity
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Science Focus: Cell Signaling
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Types of Transport: Active vs. Passive
Plasma membrane is differentially (selectively) permeable Allows some material to pass Inhibits passage of other materials
Passive Transport: No ATP requirement Molecules follow concentration gradient
Active Transport Requires carrier protein Requires energy in form of ATP
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Passage of Molecules Across the Membrane
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Types of Membrane Transport: Overview
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Types of Transport: Diffusion
A solution consists of: A solvent (liquid), and A solute (dissolved solid)
Diffusion Net movement of solute molecules down a
concentration gradient Molecules move both ways along gradient Molecules move from high to low Equilibrium:
When NET change stops Solute concentration uniform – no gradient
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Gas Exchange in Lungs: Diffusion Across Lung
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Types of Transport: Osmosis
Osmosis: Special case of diffusion Focuses on solvent (water) movement rather than
solute Diffusion of water across a differentially (selectively)
permeable membrane Solute concentration on one side high, but water
concentration low Solute concentration on other side low, but water
concentration high Water diffuses both ways across membrane but
solute can’t Net movement of water is toward low water (high
solute) concentration Osmotic pressure is the pressure that develops
due to osmosis
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Types of Transport: Carrier Proteins
Facilitated TransportSmall moleculesCan’t get through membrane lipidsCombine with carrier proteinsFollow concentration gradient
Active TransportSmall moleculesMove against concentration gradientCombining with carrier proteinsRequires energy
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Types of Transport: Carrier Proteins
Facilitated TransportSmall moleculesCan’t get through membrane lipidsCombine with carrier proteinsFollow concentration gradient
Active TransportSmall moleculesMove against concentration gradientCombining with carrier proteinsRequires energy
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Types of Transport:Membrane-Assisted Transport
Macromolecules transported into or out of the cell inside vesiclesExocytosis – Vesicles fuse with plasma
membrane and secrete contentsEndocytosis – Cells engulf substances into
pouch which becomes a vesiclePhagocytosis – Large, solid material into vesiclePinocytosis – Liquid or small, solid particles go into
vesicleReceptor-Mediated – Specific form of pinocytosis
using a coated pit
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Cell Surface Modifications: Junctions
Cell Surfaces in Animals
Junctions Between Cells
Adhesion Junctions
Intercellular filaments between cells
Tight Junctions
Form impermeable barriers
Gap Junctions
Plasma membrane channels are joined (allows communication)
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Cell Surface Modifications
Extracellular Matrix External meshwork of polysaccharides and proteins Found in close association with the cell that produced
them
Plant Cell Walls Plants have freely permeable cell wall, with cellulose
as the main component Plasmodesmata penetrate cell wall Each contains a strand of cytoplasm Allow passage of material between cells