lecture 8.slides41 77
DESCRIPTION
Chromatin Nucleotides Dynamics LectureTRANSCRIPT
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Global Local at promoters of genes
Same nucleus
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ChIPexperiments
Differential
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Taverna et al. 2007
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chromodomain
chromodomain
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Dominant negative
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Taverna et al. 2007
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Histone/chromatin crosstalk
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appears in mitosis but not interphase.
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appears in mitosis but not interphase.
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Chromatin / Nucleosome dynamics
Nucleosome folding /packaging regulatesaccessibility of DNA templates (nucleosomal DNA and
linker DNA)
The dynamics of chromatin structure are regulatedthrough multiple mechanisms including:
--histone variant incorporation--histone posttranslational modifications (PTMs)
--ATP-dependent chromatin remodeling --histone chaperones--architectural chromosomal proteins
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Tessarz and Kouzarides (2014)
Cell cycle regulated
* *
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Results
Conclusions
Jack et al (2013)
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Tessarz and Kouzarides (2014)
Lysine
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Tessarz and Kouzarides (2014)
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Nucleosome lateral surfacemodifications
-may modulate histone-DNAinteractions --> nuc stability --> nuc position stability ->increased mobility =increased accessibility
PTMs may affectnucleosome structures and
dynamics
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Tessarz and Kouzarides (2014)
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Nucleosomehistone-histonemodifications
--may destabilizetetramer-dimerinteractions --> nucstability --> histoneexchange
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Tessarz and Kouzarides (2014)
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Nucleosome facemodifications
--may modulatechromatin-bindingprotein interactionspositively / negatively
--may modulate nuc-nuc interactions -->higher order chromatinstructure
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+ respective writers, erasers, readers
+ EFFECTORS (dynamic interactions)