Biology 2060 - Microbiology for the Health Sciences SP2019 Dr. Carla Endres Office: BLT 108; phone (678-8209) or email thru Canvas or carla.endres@usu.edu

Class Website: Canvas

Lecture and Lab Schedule Lecture: M/W 3:00-4:15 PM HSL 209 Lab: T 1:00-3:50 PM HSL 208

Course: Biology 2060 provides an introduction to the biology of microorganisms with an emphasis on infectious human diseases, immunology, and the control of disease through antimicrobial strategies and vaccination. Designed for students interested in pre-nursing, pre-dental hygiene, and entry level positions in public health laboratories. Laboratory emphasizes skills with microscopes, identification testing, and aseptic technique. Suggested Pre-requisites: Biology 1010 (introductory cell biology and genetics). Lectures contain copyrighted images, many of which are objectionable and graphic. 4credits:

Lab fee: Students are charged a lab fee of $80. Fee covers the costs of media, organisms, and disposables.

Availability: Monday /Weds 8:45-9:15 AM Appointments can be made for other times.

You're invited to my online meeting during availability hours or by appointment. All centers have a dedicated computer for Acano, ask at your location.

Click to join: https://join.avc.uen.net/invited.sf?secret=an3xoixB0ONPrdfRtILMTw&id=656155431

Or call in - Video system, Jabber or Lync: office.hours@avc.uen.net

Passcode: 1234

Required Texts and Supplies: OER: https://cnx.org/contents/5CvTdmJL@4.8:rFziotaH@4/Introduction

Lab Packet (bookstore ); Three ring binder, sharpies, bring a calculator to lab, lab coat recommended..

Learning Outcomes:

1. Describe Microbial Diversity and how microorganisms can be controlled.

2. Describe pathogenic microbiological organisms with respect to epidemiology and infection

3. Perform common growth and staining techniques used in the identification of organisms and interpret the staining reactions and morphology microscopically. Identify unknowns.

4. Present a poster and oral presentation in an accurate and scientific manner.

Canvas: Canvas will be used (Web Course Tools) for the management of this class. All reserve materials, including my lectures, the syllabus, class schedule, current exam keys, and

updated grade sheets, will be available through Canvas. The reserve materials will be provided to you as downloadable PDF files, which require AcrobatReader, a free program available for both PC and Mac platforms at the Adobe web site. To access Canvas, go to the USU Eastern Blanding http://usueastern.edu/blanding/ Click on the link ‘Canvas” on the left side. Privacy is your responsibility. You will be responsible for every email and quiz submitted under your name. You will also be held accountable for every email and announcement I send through Canvas. You will want to check it regularly (at least 5 times a week). Canvas has many useful features (your assignment scores, a chat room, e-mail, etc.) and you should take the time to explore them from within our course page. I will provide more instructions on using Canvas in class.

*When you post or submit assignments into canvas, it is your responsibility to go back to make sure your post is complete and the attachments are there and visible. If they are not, you will not receive points.

Exams : There are 4 exams. Exams cover material in your text, notes, canvas and videos. 60% each are Canvas-based multiple choice; 40% are Canvas definition or problem-based. If you miss an exam because of a college-sanctioned event, officially notify me two weeks in advance. If you miss an exam because of a medical or family emergency, you must communicate this through email or direct contact. Make-up exams will involve different all long-answer essay formats. Exams will be given during class time (Testing Center on Canvas or Proctorio). Material from videos shown in class may appear on exams. Each exam will be worth 100 points. There will be no make-up exams.

Missed Exams: All students are expected to take examinations on the day they are scheduled. Only illness (verified by a physician) and extreme legitimate situations will excuse a student. If an absence can be foreseen (e.g. games, field trips, competitions etc.), the excuse must be presented prior to the event and arrangements made with me for an alternate exam time. No excuses will be accepted after “the event”. The final exam cannot be made-up without prior arrangements with the instructor.

Presentation: Students will prepare a 5 minute presentation (100 points) on a microbial organism selected by Dr. Endres. Students will use PowerPoint (PowerPoint’s must be submitted electronically by 4/1 to Dr. Endres. Students must be ready to present when called or a penalty deduction will be applied to their scores.

Project: You will be required to design and present a Research Project/Poster on a Microbiology subject (Topics to be Pre-approved by Dr. Endres). Topic: A microbial organisms and its relationship to man Projects will be presented April 13th at the 2018 STEAM Expo and will be worth 100 points.

Grading

3X100 pt. in-class exams 100 pt. final exam

Case Studies

Presentation and Project Each worth 100 pts

Written = 50 pts Oral = 50 pts

A = 90-100%; B = 80-89% C = 65-79%; D = 55-65%

F = 54% or less

Total = 400 pts Total=200 points Grand Total = 600

Scores will be posted in Canvas. Students attending regularly that receive A’s (96 % and above) on All exams and assignments, as well as complete the IDEA Evaluations, will not be required to take the final exam for the course. This does not include extra credit.

Case Studies will be available on Canvas periodically. These will be worth 10 points each and must be submitted by the due date. It is your responsibility to keep up and be aware of assignments posted on Canvas. If you do not complete an assignment by the designated due date, you will not receive points.

Extra Credit Opportunity: In the past, I have offered extra credit based on some class videos. This

year If you want to earn extra credit, your job is to find microbiology-related current news stories in any of the following sources EACH WEEK: Salt Lake, New York Times, or USA Today or another reasonable source. For each weekly article, copy and paste or scan the article - making sure the publication date and newspaper source and retrieval date are visible. Write a 1-2 paragraph summary for each, stating which microbe or group of microbes is involved and how. Describe its environmental, economic or health impact. Students that are

Failing will not be eligible for any Extra Credit, unless Professor deems them eligible.

You are to collect articles each week, not wait until the due date for this assignment to gather your articles. Articles and summaries will be uploaded into the canvas assignment.

There is NO opportunity to turn in previous packets after a given due date.

Weeks 1-3 Extra Credit Packet - Due 1/30 (5 points) Weeks 4-9 Extra Credit Packet - Due 3/13 (5 points) Weeks 10-12 Extra Credit Packet - Due 4/10 (5 points) Weeks 13-16 Extra Credit Packet – Due 4/24 (5 points)

Syllabus - Spring, 2017

Labs begin WEEK THREE

Date Lecture Chapter Lab

Module 1: Diversity

7 Jan M Introduction To Microbial World 1&2

8 Jan T NO LAB

9 Jan W Cells and Prokaryote Diversity 3 & 4

14 Jan M Eukaryotic diversity

15 Jan T NO LAB

16 Jan W Virus Diversity, Virus-Like Bacteria, Prions 5

21 Jan M No Class MLK 6

22Jan T Lab 1

23 Jan W Microbial Metabolism 7.5; 8

Jan 28 M Catch-up

Jan 29 T Lab 2

30 Jan W Exam - Introduction to Genetics

Module 2: Control

4 Feb M Microbial Growth and Control 9; 13; 14

5 Feb T Lab 3

6 Feb

W Normal Flora and Nosocomial Infections

Readings in

Canvas

11 Feb M Defenses and Immunology 17

12 Feb T Lab 4

13 Feb W Specific Immunity/Immune diseases 18; 19

18 Feb M Presidents Day No class

19 Feb T 16 Lab 5

20 Feb W Epidemiology

25Feb M Applied Immunology – Vaccines; lab analysis 20

26 Feb T Lab Recording

27 Feb W Review?

4 Mar M Lab Midterm

5Mar T

6Mar W Exam - Control to Epidemiology 11-15 Mar

Spring Break

Module 3: Pathogenesis

18 Mar M Pathogenesis 15

19 Mar T Lab 6

20 Mar W Poster Check-ups in my office

25 Mar M Respiratory Diseases - Bacterial/Viral 22

26 Mar T Lab 7/8/9

27 Mar W Skin Diseases and STD's - Bacterial/Viral 21

1 Apr

M Bug and Bite - Bacterial/Viral

Readings in

Canvas

2 Apr T Lab recording

3 Apr W Poster Work Day

8 Apr M Poster Practice and completion

9 Apr

T

Online Bacterial ID lab

10 Apr W Exam - Pathogenesis to skin

Module 4: Vectors and Parasites

12 Apr F Posters at STEAM Expo- Attendance required STEAM Expo

15 Apr

M GI Diseases - Bacterial/Viral Student Presentations

24

16 Apr T Lab Final

17 Apr

W Algal, Fungal, Protozoal Diseases Student Presentations

26

22 Apr

M Multicellular Parasites Student Presentations

25

23 Apr T

24 Apr W No Class No Class

30 Apr

Apr 25 8AM- Apr 29 noon

Final - Bug/Bite to Parasites

BIO 2060 – Microbiology Laboratory

Dr. Carla Endres Office: BLT108; phone (678-8209) or email thru Canvas or carla.endres@usu.edu Class Website: CANVAS login

Lecture and Lab Schedule Lecture: M/W, 3:00-4:15 PM HSL 206 Lab: T 1:00-3:50 PM HSL 208

Course: Biology 2060 provides an introduction to the biology of microorganisms with an emphasis on laboratory techniques and microbiological control. Designed for students interested in pre-nursing, pre-dental hygiene, and entry level positions in public health laboratories. Laboratory emphasizes skills with microscopes, identification testing, and aseptic technique Suggested Pre-requisites: Biology 1010 (introductory cell biology and genetics).

Course Objectives:

1. To demonstrate the proper handling of microbial agents. 2. To explain microbial control and metabolism. 3. To be able to identify and unknown organism with the application of learned techniques.

Lab Grading: Your lab grade will be based on laboratory worksheets (25 pts each), laboratory midterm and final. Laboratory worksheets will be due in Canvas (record results from previous lab upon arriving in lab). Lab attendance is mandatory. Arriving 10 minutes or more late will result in Missing Lab. If you miss a lab, it is still your responsibility to master the material for the exam. Individual worksheets (many with additional homework) are due 1 week after that lab was set up. No late assignments are accepted. On exams, you will be expected to sight-recognize lab images and perform calculations. During lab, you will handle some known pathogens (e.g. Staphylococcus aureus) after practice. You may also encounter unknown pathogens from personal and grocery samples. The following rules will be applied at all times to prevent contamination and promote safety. Misconduct or failure to abide by these rules in lab will result in expulsion and course failure. Absolutely NO EATING OR DRINKING IN THE LAB! Students that fail to clean their work area

before and after class will be docked 5 points for each occurrence. Students that are not prepared for lab, i.e. forgetting lab notebook, sharpies etc., will be docked 5 points for that day. You must clean up and put away all materials each day. Missing 2 or more labs will result in a Failing grade for BIOL 2060.

Exams: There will be two laboratory exams. Exams will include practical applications as well as demonstration of microbial techniques and organism identification. Exams will be worth 100 points each.

IF YOU MISS TWO (2) OR MORE LABS YOU WILL FAIL LAB and therefore Fail BIOL 2060

Grading

8X25 pt lab worksheets Unknowns 50 pt

Midterm( 100 pt) Final( 100 pt)

A = 90-100%; B = 80-89% C = 65-79%; D = 55-65%

F = 54% or less

Total = 250 pts Total=200 points Grand Total = 450

Safety and Rules in the Lab (BIO 2060) Organize lab handouts into a personal lab notebook - which you bring to every lab session, with writing tools, course text, and a calculator. Label materials carefully and keep organized records. Store other items in shelf beneath lab benches - NOT on floor or in your work area. Absolutely no drinks or food in lab. No gum-chewing, nail-biting, or nibbling pens/pencils.

Wash desktops with 10% bleach at the beginning and end of lab. Wash hands at the beginning and end of lab, and before/after using the restroom.

Follow appropriate sterile technique when handling ALL materials in lab. Handle materials with care and don't waste anything. If you spill a live culture, there will not be a back-up available.

If you spill or break contaminated materials, follow these procedures:

In personal injuries have occurred, stabilizing bleeding and clean wound first Next, disinfect spill by soaking with bleach for at least five minutes Alert me while spill is disinfecting and I will direct further clean-up

Handle and clean microscopes and prepared slides as directed; misuse will incur a loss of points. Microscopes cost hundreds to repair, thousands to replace. Prepared slides cost $10-20.

Avoiding moving microscopes; if you have to, use both hands, carrying upright and gently Clean oil from lenses with lens paper; clean stage with Kimwipe tissues and cleaner Store microscope covered with lowest power down when finished Use tissues and lens cleaner to clean prepared slides; return to front bench when clean

Small amounts of trash or waste (e.g. used pipettes) go in waste buckets on benches. Used plates, tubes, or flasks go on autoclave cart by front bench.

Equipment that can be flame/alcohol sterilized at the bench (e.g. loops, forceps, dally rods) is kept in white trays on your bench after you have appropriately decontaminated them.

Be careful with Bunsen burners. Double-check that they are off as you leave the lab.

Clean-up and put everything away before leaving lab. Make sure all culture s are in the appropriate location (incubator or autoclave bags). Remember to Flame all loops and inoculating needles after use.

Wash desktop with 10% bleach solution and allow to air dry.

Sign/date the Safety Rules Contract in Canvas PRIOR to Laboratory 1.

You must complete and agree to the safety contract in Canvas.