Lentikats BLentikats Biotechnology iotechnology

in in Food IndustryFood Industry

Content

Our aim

What is Lentikats Biocatalyst

Production of Lentikats Biocatalyst

Benefits of Lentikats Biotechnology

Lentikats Biocatalyst in Food Industry

Our guarantees

Our partners

Contacts

LentiKat’s a.s. in Food Industry LentiKat’s a.s. follows up on nine year of research and development of

Lentikats Biotechnology. Our production know-how for commercial use of our Biocatalyst and for

its industrial applications is patented internationally. Our main focus is in intensification of production processes that leads to:

• higher process yields• enhanced process stability,• Increased revenue,• Reduction of environmental footprint.

Biotechnologies are changingthe world…

Lentikats BiotechnologyUnique shape of the matrix and original immobilisation procedure enables to encapsulate free enzymes or microorganisms (bacteria, yeasts, fungi) inside a hydrogel porous carrier from polyvinyl alcohol (PVA). The advantages of PVA matrix are:

• excellent physical and mechanical characteristics that result in a long-term mechanical stability

• hardly biodegradable

• no toxic effects

Lentikats Biocatalysts enable easy separation of enzymes or microorganisms from the bulk media and therefore can be used repeatedly.

With biotechnologies to a better future…

Microencapsulation

Lentikats Biotechnology

spherical shape – limited diffusion to the “core”

traditional proven technology

new method based on well-proven principlelentil-like shape – no diffusion limitations

„micro“ size „mili“ size – easier separation

vs.

How does the Lentikats Biocatalyst work?

A maximum surface in combination with the minimum volume of Lentikats Biocatalyst results in a substantial reduction of diffusion limitations associated with the transfer of substrate into the matrix and the product out of the matrix.

Why polyvinylalcohol?

• It is an inexpensive material

• Encapsulation takes place under optimal, cell-sparing conditions

• It allows a production of hydrogel with excellent mechanical properties

• It does not have any side effects on fermentation

• It offers high capacity for the cell loading

• The process is stable and easy to control

• It is a non-toxic material

Biotechnologies are changingthe world…

How is Lentikats Biocatalyst produced?

Lentikats Biocatalysts

• Biocatalyst Types for Use in Lentikats Encapsulation:

• Free Cells• dead cells• viable, non growing cells• viable and growing cells

• Free or Immobilised Enzymes.

Lentikats Biotechnology allows to immobilise any type of enzyme

• The size of Lentikats Biocatalyst pores varies depending on the size and diameter of the Biocatalyst due to:

• PVA additive type (size of macromolecule)• additive concentration• biomass concentration

Lentikats Biocatalysts Manufacturing Plant

Laboratory Units for Encapsulation

• For low-capacity production of encapsulates (especially for research purposes), a laboratory device is available

What are the benefits?

Higher reaction rates

Reduction of reaction tanks’ volume

Operational costs savings (electricity, heat)

Lower production of waste and by-products

Environmentally friendly disposal

Matrix containing high concentration of biomass

Higher yields (in comparison to conventional technologies using free biomass)

Possibility of repeated usage

Operational costs savings

Enhanced resistance of modified and immobilised biomass

Higher process stability

Easy separation of biomass from reaction media

Reduced costs for downstream processes (separation)

..and also…

• Shortening of the reaction times

• Much higher yields in comparison to conventional technologies (up to 5 – 15 % higher)

• Repetitive use of the Biocatalyst, easy separation from media (sieve separator)

• Higher productivity of the Biocatalyst

• Lower risk of contamination during the production process

• High stability and easy control of production process

Biotechnologies are changing the world…

Technological set-up

- inlet of reaction media

- filling of Lentikats Biocatalyst

- medium with Lentikats Biocatalyst

- stirrer

- sieve separator

- outlet of separated media

- outlet of Lentikats Biocatalyst

Lentikats and food industry

We currently offer solutions for the following processes:

production of lactose free milkLentikats Biocatalyst with β-galaktosidase activity

production of invert sugar Lentikats Biocatalyst with invertase activity

whey processingLentikats Biocatalyst with β-galaktosidase activity for:

• production of D-galaktose from whey lactose (pharmaceutical quality)• production of galacto-oligosaccharides from whey lactose

production of special syrupsLentikats Biocatalyst with invertase activity for production of glucose-fructose syrupsLentikats Biocatalyst with immobilised glucoamylase for production of glucose from sugar and starch-based materials or glucose syrups from maltotriose or maltose syrup

Lentikats and food industry

Following solutions are currently under development and testing:

production of organic acids Lentikats Biocatalyst with immobilised bacteria Bacillus coagulans for production of lactic acid from lactose, glucose and sucrose

Lentikats Biocatalyst with immobilised filamentous fungi Aspergillus niger for production of gluconic acid from sucrose and glucose

Lentikats Biocatalyst with immobilised filamentous fungi Aspergillus terreus for production of itaconic acid from sucrose and glucose

Biotechnologies are changing the world…

Lentikats Biotechnology - examples

LENTIKATS BIOTECHNOLOGY FOR PRODUCTION OF INVERT SUGAR:

enzyme: immobilised saccharase, 3-D-fruktofuranosidase for hydrolysis of sucrose into glucose and fructose.

optimum temperature 30–35°C

optimum pH 4,5–5,0

conversion rate 100 %

operational stability: 600-1000 repeated batch conversions

application - processing of molasses or sucrose solutions

source activity

Baker’s yeast

600-1000 g sucrose/ kg LB/hr

LENTIKATS BIOTECHNOLOGY FOR PRODUCTION OF LACTOSE FREE MILK

enzyme: immobilised laktase, β-D-galactosid galactohydrolase for lactose hydrolysis.

optimum temperature 30–50°C

optimum pH 6,0–6,5

conversion rate > 90%

operational stability: 120–160 hours

Lentikats Biotechnology - examples

source activity

Kluyveromycer lactis

100-300 g GLC/ kg LB/hr

Investment and Investment Return Guarantees

• You can easily verify our great results by testing our Biocatalyst in your or other facilities with the material of your choice.

• Sample of Lentikats Biocatalyst free of charge

• Our company provides assistance and full technical support.

• Our company offers a long-term cooperation to companies, whose main concerns are reduced investment costs and maximal process profitability.

Biotechnologies change the world…

Investment and Investment Return Guarantees

We guarantee the following: output parameters

operational stability of Lentikats Biocatalyst and its activity and productivity

Our partners in the field of industrial applications

Fermenta Biotech, DIL Complex, Ghodbunder Road, Thane (West) - 400 610, Maharashtra, India

www.fermentabiotech.com

Protean, Pod Lesem 9, 373 16 Dobra Voda u Ceskych Budejovic, Czech Republic

www.protean.cz

Pharmix s.r.o., Na Sadkach 2798, 767 01 Kromeriz, Czech Republic

www.pharmix.cz

Our partners in the field of research and development

Fermenta Biotech, DIL Complex, Ghodbunder Road, Thane (West) - 400 610, Maharashtra, India

www.fermentabiotech.com

Institute of Microbiology, Academy of Science of the Czech RepublicVídeňská 1083, 142 20 Prague 4, Krč, Czech Republic

www.biomed.cas.cz

STU Bratislava, Fakulta chemické a potravinářské technologie, Ústav biotechnologie a potravinářství, Radlinského 9, 81237 Bratislava, Slovakia

www.stuba.sk

VŠCHT Praha, Ústav kvasné chemie a bioinženýrství, Technická 5, 166 28 Praguea 6 www.vscht.cz

University of Alberta, Edmonton Alberta, T6G 2J9 Canada www.ualberta.ca

Masaryk University, National Centre for Biomolecular Research, Kamenice 5, 625 00 Brno-Bohunice

www.ncbr.chemi.muni.cz

Thank you for attentionCompany Headquarters:LentiKat´s a.s.Evropska 423/178160 00 Prague 6Czech republic

Tel.: + 420 224 362 461Fax: + 420 224 362 463 E-mail: info@lentikats.eu

Production plant and laboratoriesLentiKat´s a.s.Pod Vinicí 83471 27 Stráž pod RalskemCzech republic

Tel.: + 420 487 851 393Fax: + 420 487 851 399E-mail: info@lentikats.eu