let’s clone a mouse
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Let’s Clone a Mouse. Somatic Cell Nuclear Transfer. Remember what you learned yesterday?. Artificial Embryonic Twinning- when a scientist mimics natural cloning by separating an embryo and allowing it to briefly develop in a P etri dish before implanting into a surrogate - PowerPoint PPT PresentationTRANSCRIPT
Let’s Clone a Mouse
Somatic Cell Nuclear Transfer
Remember what you learned yesterday?
Artificial Embryonic Twinning- when a scientistmimics natural cloning by separating an embryoand allowing it to briefly develop in a Petri dishbefore implanting into a surrogate
Somatic Cell Nuclear Transfer- when the nucleusof a somatic cell is transferred into an enucleatedegg (egg where nucleus is removed) and is thenplaced in a surrogate
Ok, so let’s begin…
On your lab bench you have 4 Petri dishes, 3 mice, 1 mouse pup, and a morula (a solid ball of cells resulting from division of a fertilized ovum).
You also have questions.
Try to answer these as you go.
Better yet, see how many you can answer now! It’s a good way to see how much you remember from yesterday’s class discussion.
Color the cells.
Color the cells as follows:Cumulus Cell Nucleus and Cumulus Cell = BlueEgg Cell Nucleus and Egg Cell = BlueMorula (ball of cells) = Green
Color the mice.
Color the 3 large female mice as follows:Somatic Cell Donor = BrownEgg Cell Donor = BlackSurrogate Mother = White
Don’t color the mouse pup yet!
Somatic Cell Donor
From the brown mouse, cut out the Cumulus Cell and place it in Petri Dish #1.
Remember, this is the mouse you are cloning!
Egg Cell Donor
From the black mouse, cut out the Egg Cell and place it in Petri Dish #2.
THINK: Do you need this mouse’s DNA?
Enucleate the Egg Cell
Cut out and discard the nucleus from the Egg Cell in Petri Dish #2. Move the enucleated Egg Cell to Petri Dish #3.
THINK: At this point, is there any DNA in the egg?
Cumulus Cell
Cut out the nucleus from the Cumulus Cell in Petri Dish #1.
Transferring…
Transfer the Cumulus Cell Nucleus into the Enucleated Egg Cell in Petri Dish #3 by taping them together on the back.
THINK: An adult mouse has 40 chromosomes. How many chromosomes does the egg cell have right now?
Waiting time!
Transfer the Egg Cell with the newly replaced nucleus onto Petri Dish #4 and let it rest for a minute. This waiting time represents the 1-6 HOURS that the new nucleus needs to adjust to the Egg Cell.
Now it’s a morula!
After it is chemically stimulated, the new Egg Cell divides into a ball of cells called a Morula. Cover the new Egg Cell with the Morula.
Surrogate
After the Egg Cell divides into a Morula, it is placed into the womb of the Surrogate Mother. After about 19 days, the Surrogate Mother will give birth to a new Mouse Pup.
THINK: Which adult mouse will the mouse pup resemble? What color will it be?
Color the newly delivered Mouse Pup this color.
Tidy your lab station- throw away your trash paper bits and place
mouse and keyboard up.
Finish answering questions.