lightrun brochure gatc
DESCRIPTION
Freestyle Max Reagent PpsTRANSCRIPT
7/16/2019 LIGHTrun Brochure GATC
http://slidepdf.com/reader/full/lightrun-brochure-gatc 1/4
L i g h t - s p e e d
s e q u e n c i n g
+
l i g h t p r i c e
WWW.GATC-BIOTECH.COM
w w w . g a t c - b i o t e c h . c o m / l i g h t r u n
N E W
s e q u e n c i n g s e r v i c e :
barcode order on l y
no sequenc ing order
free samp le s h ipmen t
Da ta de l i vered
from 8 am – 1 pm
ne x t da y!
Any qu
customers
Germany
Great Britai
France
Sweden
w w w
7/16/2019 LIGHTrun Brochure GATC
http://slidepdf.com/reader/full/lightrun-brochure-gatc 2/4
Sample requirements
Puried plasmid DNA or puried PCR fragments with primer premixed in tubes
Primer characteristics
• Themeltingtemperature(T M) of the primer should be between 52° and 58°C and
the length should be between 17 – 19bp. Ideally, the GC content of an 17mer should
be 10 G+C; for an 18mer 8 – 9 G+C and for a 19mer 7 – 9 G+C.
• GorCshouldbeatthe3’end,butnotmorethan3GsorCs.
• Theprimersequenceshouldbeagoodmixofall4nucleotideswithnomorethan
4identicalbasesinarow(AAAAorGGGG).
To ensure the best possible quality of sequence data, the total volume of the sample cannot be less than 10 µl.We recommend measuring the DNA concentration on an agarose gel. Sequencing reactions cannot be repeated andall reactions have to be paid. Your DNA material cannot be s tored. Thank you for your understanding.
Template DNA of 5 µl with either of following concentrations:•PlasmidDNA(puried):80–100ng/µl•PCRproduct(puried):20–80ng/µl
Add 5 µl of primer with following concentration:•5µM(5pmol/µl)
Pleasesendtotalsampleamountof10 µl in 1,5 ml tubes.
2.1.
Sampl
1. GATC Co
Therearem
sample ship
2. FREE shi
Ifthereisn
forshipmen
3. By post
Ifthereisn
tosend,ple
toensuresa
GATC Biote
European C
Gottfried-Ha
51105 Köln
7/16/2019 LIGHTrun Brochure GATC
http://slidepdf.com/reader/full/lightrun-brochure-gatc 3/4
Order LIGHT run sequencingbarcodes
Go to www.gatc-biotech.
com/lightrun and click on
“Order LIGHT run barcodes”.
Choose service type and order
your LIGHT run sequencing
barcodes. They will be sent to
you without delay.
LIGHT run in 5 easy steps:
Premix templateand primer
Add to your tube:
5 µl template + 5 µl primer
(accordingto
sample requirements).
Label tubes with barcodes Attach one part of the barcode
to the tube and keep the other
part for your lab record.
Afx the barco
in the front of the tu
Pleasedon‘tusepara
to seal your tube
7/16/2019 LIGHTrun Brochure GATC
http://slidepdf.com/reader/full/lightrun-brochure-gatc 4/4
LIGHT run barcode types
LIGHT run tubesdelivery time: 1 day
upon sample receipt
LIGHT run NXP tubesdelivery time: 8 am – 1pm
nextday,aftersampledrop-off
LightRunfor Tubes
Seq. ID12AA34 L
i g h t R u n
I l a b
r e c o r d
S e q .
I D 1
2 A
A 3 4
T h i s
p a
r t f o r
y o u r l a b r e
c o r d
T h i s
p a r t
f o r y o u r t
u b e s
LightRunfor Tubes
Seq. ID12AA34 L
i g h t R u n
I l a b
r e c o r d
S e q .
I D 1
2 A
A 3 4
T h i s
p a
r t f o r
y o u r l a b r e
c o r d
T h i s
p a r t
f o r y o u r t
u b e s
L i g h t R u n
I
l a b r e c o r d
S e q .
I D 1
2 A A 3 4
L i g h t R u n f o r P l a t e
S
e q .
I D
1 2 A A 3 4
T h i s
p a
r t f o r
y o u r p
l a t e s
T h i s
p a
r t f o r
y o u r l a b r e
c o r d
LIGHT run 96 well platesdelivery time: 1 day
upon sample receipt
LIGHT
Single read
For tubes: eFor 96 well
Nextdaydatad
LIGH
up
delive