lignin-depolymerization-aromatic monomers-solid acid-heterogeneous catalyst-a. k. deepa-paresh...
TRANSCRIPT
Depolymerization of lignin over
heterogeneous catalyst having acidic functionality
Presented by,
A. K. Deepa
Research guide: Dr. Paresh L. Dhepe
Catalysis & Inorganic Chemistry Division
CSIR-National Chemical Laboratory, Pune, India
Tel. 91-20-25902024, Fax. 91-20-25902633,
Email: [email protected]
Group Webpage: http://academic.ncl.res.in/pl.dhepe
Biomass Biomass can be defined as “the total mass of living organisms or recently living organisms in a given area or of a given species usually expressed as dry weight” It can be plant derived or animal derived
Biomass can be converted into high energy fuels and chemicals similar to those obtained from fossil feedstocks
Less expensive If produced in renewable basis biomass energy can reduce the net CO2 in the atmosphere thereby
reducing global warming Low concentration of sulfur help to reduce the acid rain phenomenon
Major sources of biomass Bio-refinery concept
Advantages of biomass over fossil feestocks
Biomass Sources
Animal residues
CO2
Chemicals
Energy
Fuels
Carbon sources
Biorefinery
2
Lignocellulosic biomass
1. J. Zheng, L. RehmannInt. J. Mol. Sci. 2014, 15, 18967-18984 2. Study on availability of Indian biomass resources for exploitation; Technology Information, Forecasting and
Assessment Council (TIFAC)
Lignocellulosic biomass is plant derived non edible biomass World annual production of lignocellulosic biomass is ca. 1-5x 1010 MT (metric tons)1
India produces ca. 623.4 MMT(per annum) of cropwaste (lignocellulosic material)2
Adapted from Chem. Rev. 2010, 110, 3552-3599
3
Lignocellulosic biomass contain cellulose (38-50 %), hemicellulose (23-32 % ) & lignin (15-25 %)
Adapted from, B. Kamm, P. Gruber, and M. Kamm, Biorefineries-Industrial Processes and Products, pages 165
Natural production of lignin 20 billion tons/year India produces ca. 125 MT of lignin/year Paper and pulp industry produces ca. 70 MT of Kraft lignin/year But 99 % of Kraft lignin is burnt for power generation Only about 1% of Kraft lignin are commercialized per year by MeadwestVaco in US Approximately 1 MT of lignosulfonates and 10,000 tons of soda lignin are generated from sulfite
and soda pulping industries, respectively Cellulose to ethanol produces ca. 1-3 kg of lignin as waste/kg of ethanol produced
Availability of Lignin
Aromatic nature
Abundant availability
Lignin
Value added
chemicals & fuels
4
Adapted from, Sakakibari, A., Wood Sci. Technology, 1980, 14, 89.
Structure of lignin Major linkages
5
β-O-4 4-O-5 α-O-4
β,β 5,5 β-5 β-1
C-O-C linkages: 60-70 %
C-C linkages: 30-35 %
Valorization of lignin
Lignin
Carbon fibers Polymer
Extenders Substituted lignins Thermoset resins
Composites Adhesives
Binders Preservatives
Polyols
Macromolecules
Combustion Energy
Aromatic monomers
Oxidised products
Hydrocarbon
Syngas products
Depolymerized products
6
Summary on catalytic transformations of lignin Phenol,
guaiacol, syringol (<15%)
CO2, CO, H2
Methoxy phenol, catechol, phenol
Syringol, guaiacol,
catechol(<10%)
Catechols, phenol
Hydrocarbons and gases
Vanillin, vanillic acid
Benzoquinone
J. S. Shabtai, US Patent, 5, 959, 167, 1999. Y. Kou, ChemSusChem, 2008, 1, 626. N. N. Bakhshi, Fuel Process. Technol., 1995, 45, 161. M. Goto, Chem. Eng. Technol., 2007, 30, 1113. N. N. Bakhshi, Bioresour. Technol., 1991, 35, 57. Chem Review 2010, 110, 3552
High T, Coke & gas
Homogeneous catalyst
Precious metals
High T, Coke & gas
Homogeneous acids
High T Coke & gas as
major products
Homogeneous base
High T & P coke &char
7
a. Commercial lignin: Organosolv lignin, Dealkaline lignin
b. Industrial lignin: ORG, EORG
c. Isolated lignin: Bagasse lignin (Organosolv technique)
Substrate and its properties
a M.W determined by MALDI TOF, b by GPC, c from Aldrich
Deepa et. al, ACS Catalysis, 2015, 5, 365–379
Substrate Source M.W (Da)
Elemental analysis
(%)
ICP-OES Na
(mg)
EDAX (Element
)
TGA-DTG (Residue
%)
Monomer molecular formula*
C H S N2 Air
Dealkalinea,b TCI 60,000
65 7 1 29 C, O, Na, S 36 17 C9H10.62O2.89S0.06
Organosolvb Aldrich Mn=2285 Mw=4575
P.D=2
65 6 0 0 C, O 40 2 C9H10O3
Alkalic Aldrich Mn=5000 Mw=28000
P.D=5.6
61 6 1 70 C,O, Na 30 2 C8.47H10O3.3S0.05
ORGb Industry Mn=4177 Mw=7059 P.D=1.68
57 8 0 0 C, O 34 0 C8.5H10O4
EORG Industry nd 59 5 0 1.1 C, O 36 3 C9H10O4
Bagasse lignin
Synthes-ized
nd 51 7 0 0 C, O, K, Cl 30 0 C7.9H10.1O15.9
8
Properties of solid acids (for lignin depolymerization)
aBrunauer–Emmett–Teller surface area, bPorevolume, bPorediameter [Autosorb1C Quantachrome, instrument]
dAcidity measured by means of TPD of NH3[Micrometrics Autochem-2910 instrument] .
Catalyst
Structure
Nitrogen sorption NH3-TPDd
BET SA a
(m2g-1)
V b
(cm3g-1)
D c
(nm)
Weak acid sites (mmolg-1)
Stong acid sites (mmolg-1)
Total
acidity
(mmolg-1)
H-USY (Si/Al=15) Micro 873 0.45 0.61 0.06 0.49 0.55
H-ZSM-5 (Si/Al=11.5) Micro 423 0.22 0.60 0.37 0.61 0.97
H-BEA (Si/Al=19) Micro 761 0.34 0.60 0.25 0.66 0.91
H-MOR (Si/Al=10) Micro 528 0.22 0.59 0.5 0.65 1.18
Nb2O5 -- 115 -- -- 0.30 -- 0.30
SO42-/ZrO2 -- 84 0.02 -- nd nd nd
Clay (K10) Layered 246 0.3 -- 0.09 0.33 0.42
Al pillared clay Layered nd nd nd nd nd nd
SiO2-Al2O3 Micro-meso 532 0.82 4.90 0.17 0.46 0.63
10%MoO3/SiO2 Nonporous nd nd nd 0.09 - 0.09
9
Depolymerization of lignin over solid acid catalysts
Lignin
Solid acids
T≤ 250 C, N2
H2O:CH3OH(1:5 v/v)
Zeolites Clay
Sulphated zirconia SiO2-Al2O3
O O
Si Al
O
Si
H
O
O O O O OO
Organic solvent soluble
monomers
10
Deepa et. al, ACS Catalysis, 2015, 5, 365–379 Deepa et. al, Patent Application no: IN 2889 DEL 2010, US 13/467,128, AU 2012202602, BR 102012017987-3, ES 201300399
Reaction conditions
Lignin (0.5g), Solid acid catalyst (0.5g), Solvent: H2O:CH3OH (1:5)v/v, Temp: 215-270 °C,
Time: 30-120 minutes, rpm: 500,1000 rpm, Pressure: 0.1-0.7 MPa N2 at RT.
Batch mode reactor (100 ml Parr) used for depolymerization studies of lignin
11
Reaction charge
Reaction
RM in MeOH+H2O
Centrifugation
Solid(Catalyst + coke or char)
Solution(CH3OH solb.)
Rotavap
EtOAc CHCl3 DEE
Solb.*
THF
Solb.*Solb.*
Insolb. Insolb. Insolb.
*Analyzed in GC-FID, GC-MSand
Products isolated by column chromatography
Solb.*
Insolb.
Work up procedure
H-USY gave the maximum aromatic monomer yield of 60 % with Dealkaline lignin and 35 % with Organosolv lignin as a substrate at 250 °C, 30 minutes, 500 rpm and 0.7MPa N2
H-USY catalyst was found to be deactivated in recycle runs XRD, N2 sorption, NH3-TPD, ICP-OES, 29Si and 27Al NMR showed that structural deformation and or poisoning of
of the H-USY catalysts after lignin depolymerization reaction
Dealkaline lignin(0.5g), Solid acid catalyst(0.5g), H2O:CH3OH (1:5 v/v), 250 °C, 30 minutes, 500 rpm, 0.7MPa N2 Aromatic monomers extracted using THF
Dealkaline Organosolv Catalytic results
0 20 40 60 80 100
1.18
0.97
0.91
0.63
0.55
0.42
0.35
0.3
0.09
0.45
0
Aromatic monomers (%)
To
tal a
cid
ity
(m
mo
lg-1
)
Noncatalytic SO4
2/ZrO2
10% MoO3/SiO2
Nb2O5
Al pillared clay
Clay, K10
H-USY(Si/Al=15)
SiO2-Al2O3(Si/Al=5.3)
H-BEA (Si/Al=19)
H-ZSM-5 (Si/Al=11.5)
H-MOR (Si/Al=10)
0
5
10
15
20
25
30
35
40
Aro
mat
ic m
on
om
ers
yiel
d(%
)
Catalyst
Organosolv lignin(0.5g), Solid acid catalyst(0.5g), H2O:CH3OH (1:5 v/v), 250 °C, 30 minutes, 500 rpm, 0.7MPa N2
Aromatic monomers extracted using DEE
12 Deepa et. al, RSC Adv., 2014, 4, 12625-12629
Confirmation of aromatic monomer formation
Analysis of dealkaline lignin reaction mixture, (a) GC-FID analysis and (b) HPLC analysis Reaction conditions: Dealkaline lignin (0.5 g), SiO2-Al2O3 (0.5 g), H2O:CH3OH (1:5 v/v), 250 °C, 30 min., 500 rpm, 0.7 MPa N2 at RT.
Minutes
4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
nR
IU
-10000
-5000
0
5000
10000
15000
20000
25000
30000
35000
40000
45000
50000
nR
IU
-10000
-5000
0
5000
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15000
20000
25000
30000
35000
40000
45000
50000
RID: RI Signal
12 percTHF solb 40 58 2 comp 0.8ml rep
Retention Time
Area
Width
Minutes
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nR
IU
-10000
-5000
0
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IU
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40000
45000
50000
RID: RI Signal
12 percTHF solb 40 58 2 comp 0.8ml rep
Retention Time
Area
Width
Minutes
(a)
(b)
(b) HPLC
Minutes
4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
nR
IU
-10000
-5000
0
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nR
IU
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50000
RID: RI Signal
12 percTHF solb 40 58 2 comp 0.8ml rep
Retention Time
Area
Width
Minutes
4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
nR
IU
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-5000
0
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nR
IU
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15000
20000
25000
30000
35000
40000
45000
50000
RID: RI Signal
12 percTHF solb 40 58 2 comp 0.8ml rep
Retention Time
Area
Width
Minutes
(a)
(b)
(a) GC-FID
13 Deepa et. al, ACS Catalysis, 2015, 5, 365–379
GPC analysis
0 5 10 15 20 25 30 35 40 45
-150
-100
-50
0
50
100
150
200
250
300
350
Det
ecto
r re
sponce
(a.
u)
Retention volume (mL)
Blank
MeOH soluble RM
Dealkaline lignin
To further confirm the products formed are aromatic monomers, THF soluble products were also analyzed by MALDI-TOF technique to verify that no high molecular weight fragments (1000-10,000 gmol-1) are formed.
MALDI-TOF analysis
0 5 10 15 20 25 30-40
-20
0
20
40
60
Retention volume (mL)
Det
ecto
r re
sponce
(a.
u)
THF soluble products
15 Deepa et. al, ACS Catalysis, 2015, 5, 365–379
Quantification of aromatic monomers
Minutes
4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
nR
IU
-10000
-5000
0
5000
10000
15000
20000
25000
30000
35000
40000
45000
50000
nR
IU
-10000
-5000
0
5000
10000
15000
20000
25000
30000
35000
40000
45000
50000
RID: RI Signal
12 percTHF solb 40 58 2 comp 0.8ml rep
Retention Time
Area
Width
Minutes
4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
nR
IU-10000
-5000
0
5000
10000
15000
20000
25000
30000
35000
40000
45000
50000
nR
IU
-10000
-5000
0
5000
10000
15000
20000
25000
30000
35000
40000
45000
50000
RID: RI Signal
12 percTHF solb 40 58 2 comp 0.8ml rep
Retention Time
Area
Width
Minutes
(a)
(b)
Reaction conditions: Dealkaline lignin (0.5 g), SiO2-Al2O3 (0.5 g), H2O:CH3OH (1:5 v/v), 250 °C, 30 min., 500 rpm, 0.7 MPa N2 at RT.
16
Deepa et. al, ACS Catalysis, 2015, 5, 365–379
Correlation between lignin and aromatic monomers structures and functional groups
4000 3500 3000 2500 2000 1500 1000 500
800858
1022
11071201
1265
1372
1458
1508
15941708
2852
2924
3395
Wavenumber (cm-1)
794
1022
1118
1455
1595
16903357
Dealkaline lignin
Products
% T
ran
smitt
an
ce(I) FTIR
17 Deepa et. al, ACS Catalysis, 2015, 5, 365–379
Correlation between lignin and aromatic monomers structures and functional groups
(IIA) 1H NMR (700 MHz)
18
19
Correlation between lignin and aromatic monomers structures and functional groups
(IIB) 13C NMR (700 MHz)
Comparison of catalytic activities between solid acids and homogeneous acids
Minutes
6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21
pA
0
10
20
30
40
50
60
70
80
90
100
pA
0
10
20
30
40
50
60
70
80
90
100
11
.31
9
12
.77
3
Front Signal
H2SO4 RM
Retention Time
m/z=220
m/z=166
m/z=252
m/z=234m/z=270
m/z=152
Minutes
6 7 8 9 10 11 12 13 14 15 16 17 18 19
pA
0
10
20
30
40
50
60
70
80
90
100
110
120
130
140
pA
0
10
20
30
40
50
60
70
80
90
100
110
120
130
140
11
.32
0
12
.78
1
Front Signal
HCl THF solb
Retention Time
m/z=220
m/z=166
m/z=152
m/z=252
m/z=234 m/z=270
m/z=234
Reaction conditions: dealkaline lignin (0.5 g), Acid (pH = 2), H2O:CH3OH (1:5 v/v), 250 °C, 30 min., 500 rpm, 0.7 MPa N2 at RT. Products are extracted in THF solvent.
HCl H2SO4
29 % and 39 % of THF soluble products was observed for HCl and H2SO4 respectively Few products with m/z value 152, 166, 220 corresponding to aromatic monomers, were also observed
in the non catalytic reaction. Along with this, m/z values of 252, 234, 270 which corresponds to higher molecular weight fragments were also observed.
It can be concluded that homogeneous acids like HCl or H2SO4 depolymerizes dealkaline lignin to give mainly dimers or oligomers as products, instead of giving aromatic monomers as major products under the above reaction conditions 20
Catalytic results: Optimization of reaction conditions for Dealkaline
lignin depolymerization reaction
Catalyst : SiO2-Al2O3
Temperature effect: 215 °C (1 %), 230 °C (25 %), 250 °C (29 %), 275 °C (15 %) Pressure effect: 0.1 MPa (24 %), 0.7 MPa (29 %) Time effect (@500rpm) : 30min. (29 %), 60min. (44 %), 90min. (56%), 120min. (56 %) Stirring speed (@30min.): 500 rpm (29 %), 1000 rpm (58%) Solvent effect: H2O:CH3OH (29%), H2O:C2H5OH (29 %) Solvent ratios: H2O:CH3OH (1:5)v/v (29 %), H2O:CH3OH (1:1)v/v (22 %), H2O:CH3OH (5:1)v/v (1 %) (1%) Substrate to catalyst ratio (S/C wt/wt): 1 (29 %), 2 (22 %) Optimized reaction conditions: T=250 °C; P=0.7MPa; t=90min. (@500rpm), rpm=1000 rpm (@30min.); solvent= H2O:CH3OH (1:5)v/v, S/C wt/wt=1 Catalysts were recycled upto 3 cycles with slight decrease in the activity
21 Deepa et. al, RSC Adv., 2014, 4, 12625-12629
Catalytic results Substrate effect
Dealkaline lignin, alkali lignin, bagasse-lignin, ORG and EORG lignin show ca. 60 % aromatic monomers yield.
0
10
20
30
40
50
60
70
Org
anic
solv
ent so
luble
pro
ducts
(%
)
Lignin
22 Deepa et. al, ACS Catalysis, 2015, 5, 365–379
Lignin (0.5 g), SiO2-Al2O3 (0.5 g), H2O:CH3OH (1:5 v/v, 30 mL), 250 oC, 30 min., 1000 rpm, 0.7 MPa N2 at RT Products are extracted in THF for dealkaline lignin, in DEE for organosolv lignin, in EtOAc for alkali/EORG/bagasse lignin and CHCl3 for ORG lignin
Product isolation and characterization o Product isolation was done using column chromatography.
o 3 Monomer products were isolated & confirmed using GCMS & NMR.
23 Deepa et. al, ACS Catalysis, 2015, 5, 365–379
In summary, for the first time that lignin can be converted to aromatic monomers below 250 °C using bare solid acid catalysts
Even the lignin having molecular weight of 60,000 Da was successful depolymerized into value added aromatic monomers with very high yields (60%) using solid acid catalysts under inert atmosphere
A variety of catalysts ranging from crystalline to amorphous were used in the study and it was observed that catalysts having well defined structure were prone to undergo alterations
Monomers were isolated using column chromatography (3 nos)
Conclusions
24
Solid acid catalysed depolymerization of lignin into value added aromatic monomers. A. K. Deepa and Paresh L. Dhepe, RSC Adv., 2014, 4, 12625-12629. http://pubs.rsc.org/en/Content/ArticleLanding/2014/RA/c3ra47818a#!divAbstract Lignin depolymerization into aromatic monomers over solid acid catalysts. A. K. Deepa and Paresh L. Dhepe, ACS Catalysis, 2015, 5, 365–379. http://pubs.acs.org/doi/abs/10.1021/cs501371q Depolymerization of lignin using solid acid catalysts. A. K. Deepa and Paresh L. Dhepe, Patent Application no: IN 2889 DEL 2010, US 13/467,128, AU 2012202602, BR 102012017987-3, ES 201300399. http://www.google.com/patents/US20120302796
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