limulus j test wakowako-chem.co.jp/lal/products/pdf/manuals_list/j_test.pdf · limulus j test wako...
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Limulus J Test Wako
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Table 1 JPSE m
JPSE soln. (ml ) H2O (ml ) final JPSE conc. (EU/ml )
0.5 (10000 EU/ml ) 4.5 10000.5 (1000 EU/ml ) 4.5 1000.5 (100 EU/ml ) 4.5 101.0 (10 EU/ml ) 4.0 2.02.0 (2.0 EU/ml ) 2.0 1.02.0 (1.0 EU/ml ) 2.0 0.52.0 (0.5 EU/ml ) 2.0 0.252.0 (0.25 EU/ml ) 2.0 0.1252.0 (0.125 EU/ml ) 2.0 0.0625
Table 2 CSE m
CSE soln. (ml ) H2O (ml ) final CSE conc. (ng/ml )
0.5 (100 ng/ml ) 4.5 100.5 (10 ng/ml ) 4.5 1.00.5 (1.0 ng/ml ) 4.5 0.102.0 (0.1 ng/ml ) 2.0 0.052.0 (0.05 ng/ml ) 2.0 0.0252.0 (0.0025 ng/ml ) 2.0 0.001252.0 (0.00125 ng/ml ) 2.0 0.00625
CSE m EU m
Table 3 JPSE m EU/ml g Tg min.
JPSE (EU/ml ) Tg (min.)
0.0625 50.8 49.20.0125 42.8 42.20.025 35.0 34.80.5 30.0 29.81.0 24.8 24.42.0 21.0 21.4
log (Tg (min.) ) 0.251 log (JPSE conc. (EU/ml ) ) 1.397r 0.9989
Table 4 CSE m ng/ml g Tg mean min.
CSE (ng/ml )Tg mean (min)
vial 1 vial 2 vial 3 vial 4
0.00625 49.5 50.1 57.8 51.30.0125 40.2 42.2 43.1 42.00.025 34.8 35.4 36.3 36.70.05 29.9 31.2 31.2 30.00.1 24.6 24.2 24.8 24.5
Table 5 CSE m EU
CSE d86!m Tg EU EU(ng/ml ) (min) (EU/ml ) (EU/ng)
0.00625 52.1 0.0532 8.510.0125 41.9 0.1268 10.140.025 35.8 0.2375 9.500.05 30.6 0.4439 8.880.1 24.5 1.0772 10.77
EU 9.56 (EU/ng)
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