liposomes and nanotechnology
DESCRIPTION
SEMINAR ON. SEMINAR ON ……. LIPOSOMES AND NANOTECHNOLOGY. PRESENTED BY: MAHESH DABHI. Department Of Pharmaceutics and Pharmaceutical Technology, L.M. College of Pharmacy. List of contents. Introduction: Advantages with use of liposomes as drug delivery system. Classification - PowerPoint PPT PresentationTRANSCRIPT
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PRESENTED BY:
MAHESH DABHI
SEMINAR ON
LIPOSOMES AND
NANOTECHNOLOGY
SEMINAR ON ……..
Department Of Pharmaceutics and Pharmaceutical Technology,L.M. College of Pharmacy.
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List of contents
o Introduction:
o Advantages with use of liposomes as drug delivery
system.
o Classification
o Manufacturing of liposomes
o Liposome characterization and control
o Stability consideration for liposomal formulations
o Regulatory science of liposome drug products
o Drug release from liposomes
o Applications
o Recent innovations
o Approved liposome products
o References
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INTRODUCTIONo The preparation of liposomes, with
entrapped solutes, was demonstrated for the first time in 1965 by Prof. A.D. Bangham of the United Kingdom.
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Definitiono “Liposomes are microscopic spheres made from
fatty materials, predominantly phospholipids.
o “made up of one or more concentric lipid bilayers, and range in size from 50 nanometers to several micrometers in diameter”
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Advantages with liposomes
o Suitable for delivery of hydrophobic, hydrophilic and amphipatic drugs and agents
o Chemically and physically well characterized entities
o Biocompatible
o Suitable for controlled release
o Suitable to give localized action in particular tissues.
o Suitable to administer via various routes
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Classification
o Classification based on size of liposomes
o Classification based on method of preparation
o Classification based on composition and in vivo application
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Classification based on sizeSmall unilamellar vesiclesMedium sized unilamellar vesiclesLarge unilamellar vesiclesGiant unilamellar vesiclesUnilamellar vesiclesOligolamellar vesiclesMultilamellar large vesiclesMultivesicular vesicles
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Classification based on method of preparation
o Vesicles prepared by extrusion method.o Vesicles prepared by French press.o Vesicles prepared by fusion.o Vesicles prepared by reverse phase
evaporation.o Frozen and thawed MLV.o Dehydration and rehydration vesicles.o Stable plurilamellar vesicles.
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Classification based on specific properties
o Conventional Liposomes
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Long circulating liposomes (Stealth Technology)
o PEG coating o Low permeability liquid matrix and
internal aqueous buffer system
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Targeted liposomeso Target specific ligands, such as
antibodies, immunoglobulins, lectins and oligosaccharides attached to the surface
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Cationic Liposomeso Cationic lipid component interact with
negatively- charged DNAo Results into Lipid –DNA Complexes
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Temperature sensitive liposome
PH sensitive Liposomes
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MANUFACTURING OF
LIPOSOMES
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Materials used in preparation of liposomes
o Phospholipidso Synthetic Phospholipidso Glycerolipids o Sphingolipidso Glycosphingolipidso Steroidso Polymeric materialo Charge-inducing lipids
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Structure of phospholipids
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Issues to consider when selecting lipids.
o Phase transition temperatureo Stabilityo Chargeo Lipid mixtureso Cholesterolo Source
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Preparation of Liposomes
Mechanism of Vesicle Formation
o The budding theoryo The bilayer phospholipids theory
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The budding theoryo Stress induced hydration of phospholipidso Organization in to lamellar arrayso Results in to budding of lipid bilayer leading to down
sizing
SUV OLV
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The bilayer phospholipids theory
o Liposomes are formed when thin lipid films are hydratedo The hydrated lipid sheets detach during agitation and self-
close to form large, multilamellar vesicles (LMV)
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Method of
Liposome Preparation
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Conventional liposome preparation methods
PhospholipidsCholesterol Antioxidant
Lipid component compounding Lipid solvent
Pyrogen Ultrafilteryes
No
Filter
Solvent removal
Drug ,Salt Antioxidant Buffer WFI
Filter
HydrationSolvent recovery
Extrusion Down sizing
Free drug removal
Prefilter
Sterile filter
Vial filling
Free drug recovery
Aseptic processingLyophollization Seal / package
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Method for preparation of (SUV)
o Sonicationo High shear fragmentationo Solvent injection method
o Injection of water immiscible solvent.o Ether infusion.o Fluorocarbon injection.
o Injection of water miscible solvent.o Ethanol injectiono Modified ethanol injection method
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High shear fragmentation
Aqueous samples
Piston
Cell body
Rubber-O-ring
Closure plug
Pressure relief valve
OutletFig. French pressure cell
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Solvent injection method.
Vacuumpump
Mix
Gasket
Ether/lipidsolution
Infusion pump
Aqueousphase
Mechanical drive
TemperatureControlledbath
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Large and Intermediate sized unilamellar vesicles.
o Methods used to prepare LUV and IUVo Detergent dialysiso Water in oil emulsion techniqueo Freeze thaw cyclingo Slow swelling in non electrolyteso Dehydration followed by rehydrationo Dilution or dialysis of lipids in the presence
of chaotropic ions.
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Reverse phase evaporation technique.
Lipid in solvent solution
Two-phase system Water in oil emulsion
Solvent removalGel formationREV liposomes
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High pressure extrusion.
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Methods for controlling liposome size
o Fractionationo Centrifugationo Size exclusion chromatography
o Homogenization
o Capillary pore membrane extrusion
o Ceramic extrusion
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Liposome characterization and control
Liposomes
Size Number of lamellae
Charge Stability
Preparation Raw materials
Protection
Sizing method
Hydration methods
Degree of saturation
Head group
Presence of sterols
Protecting
agents
Characterized by
Determined by
Classified by
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Physical characterization parameters
o Mean size and size distribution
o Number of lamellae
o Osmotic behavior and entrapped volume
o Internal distribution of drug
o Structural and motional behavior of lipids
o Electrical surface potential & Surface PH
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o Stabilization aspect for physical instability of liposomes
o Chemical stability
o Biological stability of liposomes
Stability consideration
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Regulatory aspectso Safety concerns: liposome
formulationo Lipid toxicity (RBC lysis)o Presence of protein and lipoprotein for
natural lipidso Residual solvento Overload of RES o Particle size
o (tail above 1 um) - Blockage of capillarieso Size affects RES uptake and tissue targeting
o Stability: shelf-live and in vivoo Dose dumping (via protein binding)o Sterility
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Drug release from liposomes
o The lipid bilayer of the liposome can fuse with other bilayers (e.g. cell membrane) thus delivering the liposome contents.
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Liposome Performance – In Vitro Release and Stability
o In vitro drug release from liposomal systems was determined using dialysis sacks.
o Release test for a targeted liposome would need to show that liposome is stable until uptake at the site.
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Factors affecting release of drug
o Solventso pHo Temperatureo Agitationo Enzymeso Cell cultureo Sink conditionso Volumeo Sampling interval
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Applications
o Liposomes as Protein Carriers in Immunology
o Oral Drug Delivery
o Site Specific Delivery
o Sustained or Controlled Delivery
o Gene Therapeutics
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Applications
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Innovations in vesicular drug delivery systems
o Provesicles in drug delivery systems
o Provesicles in drug delivery systemso Proliposomes :-o Dry granular liposomeso Mixed micellar proliposomeso Protransferosomes
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Characterization of provesicular system
o Morphology o Angle of reposeo Size and size distributiono Rate of hydration o Entrapment efficiencyo Degree of deformability and
permeability measuremento In vitro release rateo In vivo fate and pharmacokinetic
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Lipopolyplexes
o A combination of DNA, polymers and liposomes
o This method has resulted in better gene transfer and lower toxicity as compare to cationic liposomes
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Transferosomes
o Modified liposomes developed to increase the transdermal permeation of drug
o Deformability is achieved by using surface active agent in proper ratio
o Concentration of surfactant is very crucial
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Ethosomes
o Composed of phospholipids & alcohol ( ethanol or IPA)
o Sometimes polyols or glycols in relatively high concentration & water
o Better membrane permeability
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o Discomes
o Virosomes
o Emulsomes
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Cochleateso Cochleates are cigar-like microstructures
o Consist of a series of lipid bilayers which are formed as a result of the condensation of small unilamellar negatively charged liposomes.
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Depofoam technology
o Depofoam particles include hundred of bilayer enclosed aqueous compound.
o Formed by first emulsifying a mixture of an aq phase containing the compound to be encapsulated & an organic phase containing lipid.
Niosomes
o Nonionic surfactant vesicles(NSV)
o Niosomes are formed from the self assembly of non-ionic amphiphiles in aqueous media resulting in closed bilayer.
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Preparation of Liposomes by dry film method
o Lipids and drug dissolved in CHCl3
and evaporated to form thin film
o Film is hydrated with buffer solution
o Sonicated to form large unilamellar vesicles
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Preparation of Liposomes by dry film method
Lipid + drug + CHCl3
Rotary evaporation
Sonication
Thin film
LUV
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Approved liposome products marketed in US
Doxil Daunorubicin Alza Corporation
Kopasi sarcoma
Daunoxome Daunorubicin Gilead sciences
,,
Ambisome Amphotericin B ,, Serious fungal infection
Approved lipid complex products
Ambelcet Amphotericin B Alza corporation Amphotec Amphotericin B Elan corporation
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NANOTECHNOLOGY IN
DRUG DELIVERY
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List of contents
o INTRODUCTION
o NANOPARTICLES
o History
o Types of nanoparticles
o Materials and methods
o Method of preparation for Nanoparticles
o Drug loading into nanoparticles
o NANOSUSPENSION
o Method of preparation for Nanosuspension
o Purification of Nanosuspension
o CHARACTERIZATION AND EVALUATION OF
o NANOPARTICLES AND NANOSUSPENSION.
o APPLICATIONS OF NANOPARTICLES AND NANOSUSPENSIONS
o NANOSUSPENSION BASED FORMULATION IN DEVELOPMENT AND IN THE MARKET
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INTRODUCTION
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o NANOSUSPENSIONS : o are colloidal dispersions of nano
sized drug particle that are produced by suitable method and stabilized by suitable stabilizer .
o NANOPARTICLES : o They are solid colloidal particles
sized from 1-1000 nm .
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o NANOSPHERES : o polymer matrices in which drug is
dissolved or dispersed .
o NANOCAPSULES : o consists of polymer wall entrapping
an oily core in which the drug is dissolved
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History
o Developed by spieser and co-workers in the late 1960s.
o In early 1970s the cross linked polyacrylamide nanoparticles were produced.
o Widder at el incorporated magnetic particles into the nanoparticles for targeting of these particles by means of magnetic field.
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Types of nanoparticles
o Monolithic type o Capsule type
Monolithic type Capsule type
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Polymers used in preparation.
o Polymethyl methacrylate copolymers,
o Polymethyl cyanoacrylate,
o Polybutyl cyanoacrylate & Polyisobutyl cyanoacrylate,
o Polyhexyl cyanoacrylate & polyisohexyl cyanoacrylate,
o Poly(Nα,N-L-lysinedylterephthalamide
o Polyisobutyl cyanoacrylate
o Poly-D,L-lactide
o Serum albumin
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o Gelatin
o Polyacryl dextran
o Polyacryl starch
o Albumin
o Polylactic acid-poly glycolic acid copolymer
o Poly(-hydroxy butyrate)
o Ethyl cellulose
o Eudragit RL,RS
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Method of preparation for Nanoparticles
o Emulsion polymerization
o Continuous aqueous phase o One of the most rapid method
o The monomer is dissolved in an aqueous phase
o The polymerization process is initiated by -radiation, UV, or strong visible light
o Continuous organic phase
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Denaturation of natural molecules in an oil emulsion.
o Requires emulsification of a natural macromolecule and the drug
o The particles are hardened by oHeat denaturation, oCooing below the gelation point, oCross-linking with suitable reagent.
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o Disolvation of macromolecules.o This process is commonly known as
coacervation.
o Solvent evaporation
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Drug loading into nanoparticles
o Drug may be loaded at two stages-
o Prior to the preparation of nanoparticles
o Addition to the previously prepared particles
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NANOSUSPENSION
o Colloidal dispersions of nanosized drug particle produced by suitable method and stabilized by suitable stabilizer .
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Method of preparation for Nanosuspension
o Media milling (Nanocrystals)
Milling chamber
Milling Shaft
Milling media
Screen retaining milling media in chamber
Drug, water & stabilizer
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High Pressure Homogenizers
o Size reduction depends upon:
o Homogenization pressureo No. of homogenization cycle
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CHARACTERIZATION AND
EVALUATION
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Physicochemical Characterization
o Particle sizeo Molecular Weight Densityo Crystallinityo Surface chargeo Hydrophobicity o Surface propertieso Surface element analysis
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Drug release
o Side by side diffusion cells with artificial or biological membrane.
o Dialysis bag diffusiono Reverse dialysiso Ultra centrifugationo Ultra filtrationo Gel filtrationo Centrifugal Ultra filtration
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APPLICATIONS
o Oral Drug Deliveryo Parenteral Drug Deliveryo Ocular Drug Deliveryo Pulmonary drug delivery o Targeted drug delivery
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NANOSUSPENSION BASED FORMULATION
Drug Status
Paclitaxel Phase III
Rapamune Marketed
Emend Marketed
Cytokine Inhibitor Phase II
Thymectacin Phase I & II
Busulfan Phase I
Budesonide Phase I
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Referenceso A.D. Bangham , J. Mol. Biol. 13:238-252, 1965o Ali Demir Sezer,Ahmet Levent Bas, and Ju¨ lide Akbug,
Encapsulation of Enrofloxacin in Liposomes I: Preparation and In Vitro Characterization of LUV, Journal of liposome research,Vol. 14, Nos. 1 & 2, pp. 77–86, 2004
o Xuedong Yen, Gerrit L. Scherphof, And Jan A. A. M. Kamps, Liposome Opsonization, Journal of Liposome Research, 15:109–139, 2005
o Ilya Koltover, Tim Salditt, Joachim O. Radler, An Inverted Hexagonal Phase of Cationic Liposome±DNA Complexes Related to DNA Release and Delivery, 3 July 1998, vol.281 SCIENCE,www.sciencemeg.org
o Yasuyuki Sadzuka, Kaori Kishi, Sadao Hirota, and Takashi Sonobe, Effect of Polyethyleneglycol (PEG) Chain on Cell Uptake of PEG-Modified Liposomes Journal Of Liposome Research Vol. 13, No. 2, pp. 157–172, 2003.
![Page 72: LIPOSOMES AND NANOTECHNOLOGY](https://reader031.vdocuments.net/reader031/viewer/2022012308/56812d74550346895d9288f1/html5/thumbnails/72.jpg)
Referenceso Lee Leserman, Liposomes as Protein Carriers in
Immunology, Journal Of Liposome Research, Vol. 14, Nos. 3 & 4, pp. 175–189, 2004.
o Loisel S, Le Gall C, Doucet L, Ferec C, Floch V. Contribution of plasmid DNA to hepatotoxicity after systemic administration of lipoplexes. Hum Gene Ther 2001; 12:685–696.
o Pasquale Stano, Simone Bufali,Claudio Pisano, Federica Bucci,Marcella Barbarino, Mose` Santaniello, Paolo Carminati, and Pier Luigi Luisi Novel Camptothecin Analogue (Gimatecan)-Containing Liposomes Prepared by the Ethanol Injection Method, Journal Of Liposome Research, Vol. 14, Nos. 1 & 2, pp. 87–109, 2004
o Maria A Stuchlyt, Stan S StuchlyS, Robert P LiburdyO and Daniel A RousseauS, Dielectric properties of liposome vesicles at the phase Transition, Phys. Med. Biol., 1988, Vol. 33, No 11, 1309-1324.
![Page 73: LIPOSOMES AND NANOTECHNOLOGY](https://reader031.vdocuments.net/reader031/viewer/2022012308/56812d74550346895d9288f1/html5/thumbnails/73.jpg)
Referenceso Andreas Wagner, Karola Vorauer-Uhl, Gunther Kreismayr,
and Hermann Katinger enhanced protein loading into liposomes by the multiple crossflow injection technique, Journal Of Liposome Research Vol. 12, No. 3, pp. 271–283, 2002.
o Maoquan Chu1, and Guojie Liu, Preparation and characterization of hydroxyapatite/liposome core–shell nanocomposites, Nanotechnology 16 (2005) 1208–1212.
o Ellens, H., Bentz, J., and Szoka, F.C., Destabilization of phosphatidylethanolamine liposomes at the hexagonal phase transition temperature, Biochemistry, 25, 285, 1986.
o Kensil, C.R. and Dennis, E.A., Alkaline hydrolysis of phospholipids in model membranes and the dependence on their state of aggregation, Biochemistry, 20, 6079, 1981.
o Grit, M., de Smidt, J.H., Struijke, A., and Crommelin, D.J.A., Hydrolysis of phosphatidylcholine in aqueous liposome dispersions, Int. J. Pharm., 50, 1, 1989.
![Page 74: LIPOSOMES AND NANOTECHNOLOGY](https://reader031.vdocuments.net/reader031/viewer/2022012308/56812d74550346895d9288f1/html5/thumbnails/74.jpg)
Referenceso Yoshie Maitani, Hiroshi Soeda, Wan Junping, And Kozo
Takayama Modified ethanol injection method for liposomes containing _-sitosterol-d-glucoside, Journal Of Liposome Research, 11(1), 115–125 (2001).
o Shangguan et.al , 2000, Gene Ther., 7, 769.o Liposome Technology Volume 1 : Gregory Gregoridis
Pg No . 250 – 255 . o Pharmaceutical Dosage Form : Disperse System Vol . 3
Pg No . 148 – 150 .o Encyclopaedia : Pharmaceutical Technology Vol 9 Pg
No . 1 – 38 .o Modified Release Drug Delivery Technology Cha. 58
Stealth Technology .o Modified Release Drug Delivery Technology Cha. 47
SLN and Lipopearl for Topical and Controlled Release .
![Page 75: LIPOSOMES AND NANOTECHNOLOGY](https://reader031.vdocuments.net/reader031/viewer/2022012308/56812d74550346895d9288f1/html5/thumbnails/75.jpg)
Referenceso Nanosuspensions : A promising drug delivery strategy
from JPP , Vol 56 , 2004 , Pg No. 827 – 840 . o Microencapsulation : Methods and Application by
Simon Benita Cha . 10 , 11 , 12 .o Drug Delivery and Industrial Pharmacy : Liposomal as
a Drug Delivery System . 1989 Pg No. 1523 , 1989 Pg No. 197 , Pg 387 Vol 3
o Depofoam : JPS Nov 1998 , pg 1341 .o Eastern Pharmacist : March 1994 , Pg No. 61 .o Indian Journal of Pharmaceutical Sciences : Jan-Feb
2004 Pg 121-122 .o Journal of Pharmaceutical Science : Vol 90 , No.6 , June
2001 .o Journal Of Pharmaceutical Science : Vol 92 , No.1,Pg 61-
64 January 2003 .
![Page 76: LIPOSOMES AND NANOTECHNOLOGY](https://reader031.vdocuments.net/reader031/viewer/2022012308/56812d74550346895d9288f1/html5/thumbnails/76.jpg)