live food culture
TRANSCRIPT
Speaker: Dr. Malobika Das Trakroo
Live Food (Living nutritious Live Food (Living nutritious Capsules)Capsules)
• Cell SizeCell Size
• Bio-chemical CompositionBio-chemical Composition
• Toxic CompoundsToxic Compounds
• DigestibilityDigestibility
Organisms Tubifex
Chironomous larvae
Infusoria
Zooplankton
Micro algae
Application
Hatchery Operation
Aquarium Industry
Water Renovation
Closed Life Support System
Single Cell Production(SCP)
Micro algal cultureBivalve Molluscs (Clams, Mussels, Oysters, Scallops, throughout life)
Young stages of Shrimps
First feeding stage of some fishes
Small invertebrates i.e. Rotifers and Artemia
Live Feed For
Aspects of Micro Algal Aspects of Micro Algal CultureCulture
• IsolationIsolation
• Culture Media Selection & PreparationCulture Media Selection & Preparation
• Stock Culture MaintenanceStock Culture Maintenance
• Mass CultureMass Culture
• HarvestHarvest
Isolation
Methods
Pipette
Centrifuge
Agar Plating
Exploiting Phototactic Movement
Serial Dilution Culture Technique
Culture MediaCulture MediaFew Common Media:Few Common Media:
• BBM (Bold’s Basal Medium)BBM (Bold’s Basal Medium) For Green For Green algaealgae
• Conway or Walne’s MediumConway or Walne’s Medium For Micro algae For Micro algae ( Phytoflagellate)( Phytoflagellate)
• Guillard’s F/2 MediumGuillard’s F/2 Medium For MicroalgaeFor Microalgae
• TMRL MediumTMRL Medium For Diatoms For Diatoms
• SD IISD II For Green algaeFor Green algae
• DY IIIDY III For Flagellates For Flagellates (Crysophytes & Cryptomonas)(Crysophytes & Cryptomonas)
• BG IIBG II For Blue green For Blue green algaealgae
Growth Phase of Algal
Phase Declining
Stationary
Death
Lag
Exponential
Stock Culture MaintenanceStock Culture Maintenance
Stock culture room is a small AC room adjacent Stock culture room is a small AC room adjacent to mass culture room.to mass culture room.
Material Required:Material Required:– Haufkin culture flaskHaufkin culture flask– Nutrient mediaNutrient media– InoculumInoculum– IlluminationIllumination– Controlled temperatureControlled temperature
Strict anti contamination procedure has to be Strict anti contamination procedure has to be followed.followed.
Mass CultureAC room with light arrangement and centralized
aeration system.
1.ContainersGlass carbuoys (20 l)
Polythene bags (10 l)
Perspex tanks (100 l)
Fiber glass tanks (250 l)
2. Inoculum Size 250 ml for Glass carbuoys
100 ml for Polythene bags
2 l for Perspex tanks
3. Illumination3. Illumination
4. Temperature control4. Temperature control
5. Aeration5. Aeration
6. Glass wares6. Glass wares
Harvest and Preservation
Wet
Dry
FlocculationHarvesting
Preservation
Rotifer Culture
Brachionus Culture System
Raising Brachionus Culture
Raising appropriate food organism
Mass Production
Harvesting
Raising Food Organism
ChlorellaCulture
Continuous Culture
Feed back Culture
Daily Tank Transfer Method
CIFE Method
Mass Production
Batch Culture
FeedbackCulture
Semi continuous Culture
Out Door Culture Method
Slurry Tank(300 l)
10 kg chicken dropping+
5 kg GNOC+
2.5 kg SSP
FilteredWater(250 l) Aeration
Mass Culture Tank(300 l)
5th day
7th day
10th day
Rotifer100-150 N/ml
Moina20-25 N/ml
Daphnia10-20 N/ml
InoculationRotifer
( 30-40 N/ml)
Moina(10-15 N/ml)
Daphnia(10 N/ml)
3rd day
3 ml/l from 4th day4 ml/l for 3 days
Moina CultureMoina Culture
Stock RaisingStock Raising
Mass CultureMass Culture
HarvestingHarvesting
Mass Culture
Outdoor Culture (CIFE)
Application of ManurePoultry Manure4 gm/l weekly
Cow dung Substrate10 gm/l
Microbial Water100 gm PM/l
Anaerobically treated
Filtered extract 10-20l/1000l
Anaerobically digested
HarvestingHarvesting
Non selectiveNon selective
SelectiveSelective
AlternateAlternate
Infusoria Culture
By
Using Banana peelings
Using Hay infusion
Using Apple snail