live stock bioeragroup full vers

3 activity areas of BioEra Group

3

Products for livestock breeding

Products for humans

Products for agriculture

diversified biotechnology company for the production of:

A broad range of organic vitamin supplements added to combined fodder which is used in all major agricultural production segments (cattle breeding, milk production, poultry farming, fish farming) and based on biomass generated by microalgae chlorella

New-generation bioorganic fertilizers on the basis of vermicompost (biohumus) and microalgae biomass.

Production of "Live vitamins" for human - complex preparations with a full complex of vitamins and amino acids based on biomass generated by microalgae chlorella

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Biovitamin supplement for animals with full complex of essential vitamins and

amino-acids

Bioproducts

For stock raising

6Bioproducts for live stock

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Original liquid preparation of green color- suspension

iclusive of 2 gr dry microalgae Chlorella

(Chlorella vulgaris)

The most common animal diseases:

• Avitaminosis• Virus diarrhoea• Infectional rinitracheit• Mastitis• Undulant fever• Parturient paresis• Liver cirrhosis• Metabolic diseases• Toxic dystrophy

• Allergic reactions• Pneumonia• Aerocystitis• Dyspepsia• Gout• Cloasitis• Catarrh craw• Emaciation • Poisoning• Craw stoppage• Oviduct inflamation

In industrial conditions and simulated feeding animals very often undergo through this diseases.

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Inclusion into the animals diet of a living plant

Millions of years of evolution in animals 'organism were green plants. In industrial animal husbandry livestock does not get

plant food. Therefore, the use of Chlorella in animal husbandry is a crucially important step.

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А также витаминыА, D и В12в чистом виде

Chlorella vulgaris

55% Protein

12% Lipids

32% Carbs

These elements work in complex!

Chlorella – microalgae which has huge reservoir of chlorophyll and complex of rare nutritional substances, which contribute to the process of photosynthesis.

There is no other plant unit containing vitamins and minerals in a such amount. Which makes Chlorella truly unique.

Components: Chlorella vulgaris Beijer

Protein 52,8

Carbohydrates 7,3

Lipids 20,0

Carotenoids (mg%) 255

Carotene (mg%) 423

Vitamin E mg% 93,5

Vitamin B1 mg% 1,7

Vitamin B2 mg% 11,3

Nitrogen 8,45

Phosphorus 20350,0

Potassium 19720,0

Magnesium 12090,0

Copper 43,50

Calcium 18910,0

Selenium 0,5

Zinc 209,50

Iron 568,1

Leucine 3,19

Isoleucine 1,33

Lysine 1,90

Methionine 0,17

Phenylalanine 2,02

Tyrosine 2,02

Threonine 1,90

Valine 2,09

Arginine 2,11

Histidine 0,72

The amount of essential amino acids

12,73

Table 1 - Biochemical characteristics (% dry matter) Chlorella vul. Beijer

Table 2 - Mineral composition (mg / kg dry matter) Chlorella vul. Beijer

Table 3 - Amino Acid Composition (% dry matter) Chlorella vul. Beijer

Senior Research Fellow Laboratory of Analytical Chemistry, Ph.D. Federal IBS SB RAS

Gribovskaya I.V.

Head. lab. Analytical Chemistry, Ph.D. Federal IBS SB RAS Kalacheva G.S.

Components: Chlorella vulgaris Beijer:

16 vitamins and 8 minerals

19 amino acids (including 9 irreplaceable)

The highest concentration of RNA / DNA nucleic acids

Food cofactors are: enzymes, polysaccharides, sterols

Essential fatty acids - Omega-3, Omega-6

Chlorella is …

The richest source of chlorophyll

Enhancing the immune system

Improving product quality by eliminating the need antibiotics

Easily digestible protein piece

Inclusion into the diet a living plant

There is no other plant crop that contains in an amount of vitamins and minerals. What makes Chlorella truly unique.

.

Recomendations on feeding Chlorella

Tipe of animal Suspension norm by head, ml Feeding term, day

Cattle

Cows

Before copulation 1000 10

Pregnancy 1000 30

Lactation 1000 50

Calfs 300-500 30

Pigs

Before copulation 1000 10

Pregnancy 1000 30

Lactation 1000 50

Piglets 200-300 21

Poultry

Adult 30 everyday

Young 5-30 everyday

Results of experiements 2004y. Algobiotechnologies

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The results of research

In 2015, we conducted experiments in FGBNU "All-Russian Research Institute of Physiology, Biochemistry and Nutrition of Animals" on

broiler chickens, laying hens and pigs, and received the results which confirm the beneficial properties of the preparation Algavet.

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AlgaVet. Chicken Broilers. Hematologic parameters

Indicators Experimental(OP + 1 ml / kg)

Experimental(OP + 2 ml / kg)

Control (ОР)

Leukocyte count, thousand cells / mm

26,8±1,34 28,3±0,85 28,3±1,71

The number of red blood cells, million cells / mm

2,80±0,16 2,61±0,03 2,51±0,13

Hemoglobin, g / l 82,8±1,73* 79,0±2,81 77,6±1,49

Leukocyte formula %

• Basophils 2,33 1,67 1,75

• Eosinophils 5,33 6,50 7,75

Neutrophils:

• Band cell 0,33 1,00 0,50

• Segmented 24,34 23,83 24,00

Lymphocyte 63,67 62,00 63,50

Monocytes 4,00 5,00 2,50

Note: * -р < 0,05

The results of studies approved in 2015 the director of the Russian Scientific Research Institute of Physiology, Biochemistry and Animal Nutrition A.S. Ushakov.

Hemoglobin increased by 6.7%

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AlgaVet. Indicators of non-specific resistance of broiler chickens


Indicators Experimental(OP + 1 ml / kg)


Control (ОР)

Phagocytic activity,% 65,0±2,24** 55,0±1,82** 49,3±1,95

Phagocytic index 5,90±1,19 6,16±0,43* 5,21±0,97

Serum bactericidal activity, % 75,62±5,64 75,05±5,97 68,92±4,15

Lysozyme content in blood serum, g / l 7,2±0,24** 7,0±0,53 6,9±0,4

Note: * -р < 0,05, ** - р < 0,01

Phagocytic activity of white blood cells - the ability of phagocytescapture and eliminate pathogens, trapped in the body.

Cells phagocytes perform protective function, they are capablerecognize the "enemy" to capture and digest.

Phagocytic activity increased by 31.8%

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Phagocytic activity increased by 31.8 %


High biological activity of strains of Chlorella effectively manifested in the prevention of diseases that are caused by mycotoxins. Up to 10% of the total

number of chickens dying from diseases that can be cured. Moreover mycotoxins in foods in the residual amount of a danger to human health.

Chlorella suspension - is a natural probiotic capable of metabolizing mycotoxins into harmless products.

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AlgaVet. Biochemical parameters of blood serum of broiler chickens

Note: ** -р < 0,01

Group Total protein, g / l

Albumin, g / l Glucose, mmol / l

Iron mmol / l

Calcium, mmol / l

Phosphorus, mg / l

Experimental(OP + 1 ml / kg) 24,54±3,40 20,65±1,8 11,14±1,3 19,09±0,64** 1,72±0,1 2,24±0,1

Experimental(OP + 2 ml / kg) 33,64±3,08** 20,85±2,3 10,47±2,1 17,22±0,42 1,75±0,2 2,26±0,02

Control (ОР) 25,45±3,08 21,00±2,2 12,06±1,6 17,22±0,17 1,73±0,1 1,98±0,2


Phosphorus increased 13.1%

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AlgaVet. Intestinal microflora of broiler chickens

The group of microorganismsGroup of broiler chickens

Experimental(OP + 1 ml /kg)

Experimental(OP + 2 ml /kg)

Control (ОР)

Bifidobacteria, х 109 5,1±0,50* 3,3±0,46 3,06±0,06

Lactobacilli, х 108 6,8±0,06 9,6±0,74 3,7±0,44

Enterococci, х 106 1,1±0,20 1,2±0,74 1,2±0,17

Escherichia, х 106 2,4±0,37 2,1±0,16 2,1±0,33

Salmonella, х 103 - - -

Easts Candida genus ,х 103 - - -

Note: * -р < 0,05

ALG

AVET


The number of lactobacilli increased by 83.7%

The number of bifidobacteria increased by 66.6%

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AlgaVet. Live weight of broiler chickens by periods

AgeThe average live weight of 1 birds , kg

Control (ОР)Experimental

(OP + 1 ml / kg)Experimental

(OP + 2 ml / kg)

7 day 0,122 0,132 0,124

14 day 0,286 0,306 0,293

21 day 0,545 0,583 0,588

28 day 1,054 1,078 1,105

35 day 1,782 1,768 1,727

42 day2,12±0,31

100%2,25±0,37*

105,7%2,21±0,29

103,9%

Note: * -р < 0,05


Weight gain increased by 5.7%

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AlgaVet. Results. Zootechnical parameters

Note: * -р < 0,05

Group Actual weight, % Carcass yield , % The yield of the pectoral muscle

The cost of feed per 1 kg of

weight gain, kgAverage daily gain, gramm

Experimental(OP + 1 ml / kg) 100% 71,1±0,3 16,2±0,5 1,648 60,5

Experimental(OP + 2 ml /kg) 100% 73,4±0,6* 18,7±0,6* 1,677 59,6

Control (ОР) 100% 70,5±0,8 15,9±0,6 1,749 57,1


The yield on the gutted carcass increased by 4.1%The yield of the pectoral muscle increased by 7.6%

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AlgaVet. The quality of broiler meat

Indicators


Experimental(OP + 2 ml / kg) Control (ОР)

pectoral muscle

thigh muscle

pectoral muscle

thigh muscle

pectoral muscle

thigh muscle

Dry substance, % 27,52 23,87 28,31 25,24 29,6 24,46

Composition o

f drysubstances

Fat, % 14,54 16,32 12,52 17,24 12,82 14,5

Nitrogen, % 13,81 13,52 14,00 13,25 14,37 13,53

Protein, % 86,31 84,50 87,50 82,81 89,81 84,56

Caloric content, MJ/kg

23,75 24,01 23,26 24,37 22,55 23,98


The meat has become more tender and succulent!

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Egg-laying qualitieshas increased

27.1%

AlgaVet

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AlgaVet. Laying hens. Hematologic parameters.

Indicators Experimental(OP + 1 ml / kg) Control (ОР)

Leukocyte count, thousand cells / mm 30,4±0,16* 29,5±0,37

The number of red blood cells, million cells / mm 2,07±0,07* 1,91±0,09

Hemoglobin, g / l 94,8±1,03** 83,5±1,57Leukocyte formula %

•Basophils 1,67 1,75•Eosinophils 6,33 5,25Neutrophils: •Band cell - -•Segmented 29,01 26,00Lymphocyte 57,99 62,50Monocytes 5,00 4,50

Note: -р < 0,05


Hemoglobin increased 13.5%, erythrocytes - 8.3%

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AlgaVet. Indicators of non-specific resistance of laying hens.

Примечание: -р < 0,05, ** - р < 0,01


Phagocytic activity,% 58,3±3,33* 46,7±3,33

Phagocytic index 6,15±0,19** 5,09±0,21

Serum bactericidal activity, % 45,95±0,45 36,46±1,09

Lysozyme content in blood serum, g / l 4,1±0,22* 3,9±0,4


Phagocytic activity increased by 24.8%

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AlgaVet. Biochemical parameters of blood serumlaying hens

Примечание: *- р<0,05, ** - р<0,01

Groups Total protein, g / l

Albumin, g / l Glucose, mmol / l

Calcium, mmol / l

Phosphorus, mg / l

Experimental(OP + 1 ml / kg) 50,5±3,9 24,8±0,6** 17,1±1,0* 2,2±0,2* 6,2±0,8

Control (ОР) 57,6±3,0 30,3±0,3 20,7±0,7 3,1±0,3 7,3±0,3


Higher intensity of metabolic processes in the body layers, the resulting suspension Chlorella vulgaris ... can be judged at a low content of glucose in their blood, which

is the main source of energy expended metabolic processes in animals of this group more rapidly than the control. Lower content of blood serum of

experimental hens micronutrients such as calcium and phosphorus, micronutrients explained expenditure of more intensive egg production compared with the

control.

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AlgaVet. Intestinal microflora in laying hens

The group of microorganisms

Group of hens

Experimental(ОР+1 мл/кг)

Control (ОР)

Bifidobacteria, х 109 3,7±0,55 1,2±0,29

Lactobacilli, х 108 31,5±9,78 2,9±0,58*

Enterococci, х 106 4,25±0,25 0,45±0,25**

Escherichia, х 106 0,1±0,00 1,9±1,55

Salmonella, х 103 - -

Easts Candida genus , х 103 0,8±0,07 1,6±0,48

Note: * -р < 0,05


The number of bifidobacteria increased 3.5 times, Lactobacilli - 10 times

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The number of eggs laid by an average hen increased by 27.5%


Save, % 66,7 76,7

Gross harvest of eggs 1245 1064

The number of eggs laid by the initial hen 41,5 35,5

The number of eggs laid by the average hen 50,81 39,85

Average egg weight, g 60,2 61,05

The cost of feed for the production of eggs, kg of feed per 1 kg of egg mass 2,33 2,93

The cost of feed for the production of eggs, kg of feed 1000 eggs 140,5 179,1

AlgaVet. Zootechnical parameters of laying hens

The results of studies approved in 2015 the director of All- Russian Scientific Research Institute of Physiology, Biochemistry and Animal Nutrition A.S. Ushakov.

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Due to enlargement of the natural immunity of the animals

Mortality of young animals reduces

by 4-5 time

Profitability of animal husbandry increases

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Increase in the average daily weight gain during the fattening of cattle

up to 30-40%

+ 35%

Increase in milk production

up to 25%

+25%

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In present-day pig-breeding production about one third of organic substance that comes with the feed doesn’t digest. As a result accure very crucial task: decrease of losses by increase of

digestability and amelioration of digested nutrient substence use.


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According to the research of All-Russian Research Institute of phisiology, biochemestry and animal nutrition it was found that one of the solutions is supplement in piglets’ ration feed «AlgaVet».

Reinforce animals’ metabolic processes

Study report confirmed in 2016 by Doctor of Veterinary science, Professor of the Phisiology and Chemestry Faculty at the PGBOU VO Kursk GAA, sientific consultunt LLC «BioEra Group», Naumov M.M.

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AlgaVet. Feeding programme for piglets in weaning period, 2015-2016y.

Begining of the experiment

Groups Age, days Quantity, pcs Weight, kg Average weight 1 head, kg

Control (ОР) 19 324 1983,5 6,1

Experimental(ОР+AlgaVet)

19 340 1898,5 5,6

End of the experiment

Control (ОР) 70 310 6496,5 20,90


70 329 6873,0 20,93

(♂ Danish Yorkshire ×♀ Danish Landras)


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+ 8,6%

Groups Defect, pcs Mortality, pcs Average daily gain, kg

Survivability, %

Control (ОР) 3,5 10,5 0,285 95,64


2,5 8,5 0,309 96,76

AlgaVet. Average daily gain and Survivability of piglets, 2016 y.

Average daily gain increased by 8,6%.


(♂ Danish Yorkshire ×♀ Danish Landras)

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Mortality of piglets reduced by 20%, Survivability increased up to 96,76 %


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This is related to the fact that live cells of microalgae make for growth-supporting microenvironmen for intestinal micro-

organisms, which results in reinforsment of feed digestibility.


+ 8,6%

AlgaVet. Average daily gain increased

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Production of Chlorella biomass is enriched by Selenium (Se) which is useful in animal nutrition

This study has been achieved within FAO camel project UTF/SAU/021/SAU with the support of Camel and Range Research Center (CRRC).

http://www.sciencedirect.com/science/article/pii/S1018364713000736

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More efficient effect of supplementation with organic selenium compared to non-organic was confirmed on camels. Organic selenium allows a better protection of the new-born camel by transfering it into milk just after parturition.

Since young camels are often affected by sudden death due to selenium deficiency, a ration with Se should be prescribed to mother-camels during pregnancy and lactation. Moreover, as the Se supply by milk decreases regularly after delivery, the supplementation must be renewed in young camels after weaning.

Production of Chlorella biomass is enriched by Selenium (Se) which is useful in animal nutrition

This study has been achieved within FAO camel project UTF/SAU/021/SAU with the support of Camel and Range Research Center (CRRC).

Chlorella in fish-husbandry

Crustacean Biology Laboratory, Department of Zoology, Bharathiar University, Coimbatore, Tamil Nadu, India, 2013

Reduction of performance indicator(weighing, survivability, health) resulting from:

• Bloom of blue-green algae (Aphanizomenon, Anabaena, Microcystis)

• Discharge of toxines, which appear due to blue-green algae dieaway and lead to the death of fish

• Deterioration of oxygen and hydrochemical regimen

Chlorella in the basins algaelisation

From reports of Manokhin, 1977; Musapharov,1977; Bogdanov, 2005

To prevent blue-green algal nuisance planktonic Chlorella Strain is infused.

It sufficiently consolidates fish forage supply.

As a result fish capacity of ponds increases.

Chlorella in weighing and survivability

From reports of professor Bogdanov, 2005

Chlorella suspension was used in nurturance of carps’ fingerling in Sebistone Creek in Nurek reservoir. The experiment took place in 3 net pens during 100 days.1st recieved feed with silk worm pupae2nd recieved feed with silk worm pupae chlorella suspension-wet3rd was control, fish wasn’t fed

Index 1 st(silk worm pupae )

2 nd(SWP+Chlorella) Control

Average weighing 38 45 8

Survivability 87 90 41

Average weighing % in reference to Control 475% 562,5% 100%

Survivability % in reference to Control 212,1% 219,5% 100%

I.e. The best stock was recieved with treatment of Chlorella suspension.

Chlorella for fishes’ gastro-intestinal tract (GIT) support

Norwegian Centre of Excellence, Aquaculture Protein Centre (APC)

Soybean meal was used as a model to study gut health, because most of the feed in aquaculture is based on soy.

Feeding soybean meal results in:

• Enteritis in the distal intestine

• Reduced feed intake, growth, digestability

• Reduced enzyme activity and bile salt levels

in the intestine

Supplement of Chlorella in feed has beneficial effects on GIT.

Report on food supplement for Tilapia Nile with different percentage of Chlorella

Central Laboratory for Aquaculture Research, Agricultural Research Center, Ministry of Agriculture, Egypt, 2008

Feed with 50% content of Chlorella resulted in 16% weight increase of Tilapia Nile

+ 16%

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The profitability of animal husbandry increases

Feed conversion is increased due to the digestibility of feed.

Saving up to 22% of feed expenses

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Fill the shortage of green fodder in the diet by the drinkers, or as a part of mixed fodders

Get more meat products. Reduce mortality of the young animals Increase chicks’ hatchability Improve conditions of internal organs and commercial quality Fortify the immune system since it is natural probiotic

Use of chlorella hepls to :

BioEraGroup is a diversified biotechnological enterprise specializing the production of:

A broad range of organic vitamin supplements added to combined fodder which is used in all major agricultural production segments

Production of "Live vitamins" for human - complex preparations with a full complex of vitamins and amino acids

New-generation bioorganic fertilizers based on environmentally friendly standards;

Liquid bioorganic fertilizers (concentrates and extracts) for tomatoes, cucumbers, peppers, etc. cultivated in closed systems (greenhouses);

Organic fertilizers for forest nurseries, gardens, parks (including turf grasses);

+7(499)322-91-91 [email protected]

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