luciferase a. cengiz Öztireli. bioluminescence bioluminescence (living light) milky sea
TRANSCRIPT
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LUCIFERASE
A. Cengiz Öztireli
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BIOLUMINESCENCE
BIOLUMINESCENCE (LIVING LIGHT)Milky Sea
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BIOLUMINESCENCE
Applications
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BIOLUMINESCENCE
Sea AnemonesBacteria Dinoflagellates
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BIOLUMINESCENCE
FIREFLYES (PHOTINUS)Attracting matesAttracting prey
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LUCIFERASE
FIREFLY BACTERIA
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BIOLUMINESCENCE
8 ATP → 1 Green Photon
Reaction:
LuciferinLuciferase + O2 Inter. P* P + hv
ATP
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BIOLUMINESCENCE
Color P*
P* + F → F* + P Proteins around Structure
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PHOTINUS PYRALIS (FIREFLY)
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PHOTINUS PYRALIS LUCIFERASE
Luciferase + Luc-COOH
ATP
Luciferase: Luc-CO-AMP
O2
Luciferase: Luc=O*
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PHOTINUS PYRALIS LUCIFERASE
N-Terminal
C-Terminal
Cleft
Structure and Functioning
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CONCLUSIONS
THANK YOU