Lullaby - siRNA transfection reagenta sweet song for gene silencing

The art of delivery systems

Examples of cells successfully tested

Cell Lines Cell Type Species

293, 293T

3T6

A549

B16-F10

BEAS-2B

BHK-21

CHO, CHO-K1

COS-1, COS-7

CV-1

H441

HEK293

HeLa, HeLa-S3

M1

MCF-7

MDCK

MiaPaCa

N2A

NIH-3T3

PC-12

U87

Vero

Kidney

Embryonic Fibroblast

Non-small cell lung carcinoma

Melanoma

Bronchial epithelial

Kidney

Ovary (epithelial-like)

Kidney

Kidney (fibroblast-like)

Lung epithelial

Kidney

Cervix carcinoma

Kidney epithelial

Breast adenocarcinoma

Kidney

Pancreatic carcinoma

Neuroblastoma

Fibroblast

Adrenal pheochromocytoma

Glioma

Kidney

Human

Mouse

Human

Mouse

Human

Hamster

Chinese Hamster

Green Monkey

Monkey

Human

Human

Human

Mouse

Human

Dog

Human

Mouse

Mouse

Rat

Human

Green Monkey

Please visit www.ozbiosciences.com for a complete updated list of cells successfully transfected with

Lullaby®

w w w . o z b i o s c i e n c e s . c o m

Parc Scientifique de Luminy - Marseille 13288 Cedex 9 - France tel: +33 (0) 486 948 516 - fax: +33 (0) 486 948 515

Catalogue Numbers

LL70500

LL71000

LL73000

Lullaby® 0.5mL

Lullaby® 1mL

Lullaby® 3x1mL

Up to 1000 transfections

Up to 2000 transfections

Up to 6000 transfections

DreamFect™ transfection reagent andMagnetofection™ reagents (PolyMag and SilenceMag)

are examples of innovations from OZ Biosciences.Contact us to learn more about these products.

Optimized protocols for specific cell types and / or applications are

available upon request.

®

Technical Support: tech@ozbiosciences.com

Customer Service:contact@ozbiosciences.com

Control Lullaby ®5nM siRNA

Lullaby ®25nM siRNA

fig. 1: GFP-stably transfected HeLa cells treated in 24-well plate with 1 or 3µL of Lullaby® and 5 or 25nM of siRNA (targeting GFP) respectively. GFP expression monitored after 72h.

Based on an innovative technology, this new lipid-based reagent achieves extremely efficient siRNA delivery into cells. Lullaby® protects siRNA from extracellular degradation and has an outstanding capacity to destabilize cells membrane, allowing delivery of important siRNA amounts into the cytosol. Transfection is very efficient and highly reproducible, even with low doses of siRNA. Lullaby® allows high gene silencing levels in various conditions: cells, concentrations, targets, culture conditions, etc.

It has been clearly shown that high concentrations of siRNA ( > 100nM ) can cause off-target effects. Thus, 1 to 20nM siRNA concentrations are ideal for transfection. Lullaby® is efficient starting from 0.1nM of siRNA and optimal at 5 to 10nM, avoiding non-specific effects.

Effective at multiple siRNA concentrations - even with low doses

Lullaby® has been successfully tested on various cell lines, reaching up to 90% gene silencing.

Please see next page for a detailed list of cell types tested, or visit www.ozbiosciences.com for an updated list.

Powerful for all cell types

Do not worry about experimental conditions: Lullaby® is antibiotics- and serum-compatible and works over a broad range of cell confluence (between 20 to 90%).

Flexible and adapted to all culture conditions

This reagent has been tested on various RNAi targets and with synthetic siRNA from different suppliers. With a correctly designed and purified siRNA, you will get the best from Lullaby®.

This versatile reagent is suitable for all siRNA applications including endogenous targeting and co-transfections. Lullaby® can be used with siRNA, shRNA and dsRNA.

siRNA targets tested: GAPDH, GFP, Kinase, LacZ, Lamin, Luciferase.

Versatile and convenient for all siRNA applications

Lullaby® offers complementary features allowing you to benefit from state-of-the art in siRNA transfection.

Provided with a detailed protocol, you will confirm that Lullaby® out-performs competitors' products for efficiency, non toxicity and variety of applications.

Lullaby® is fully applicable to High-Throughput Screening.

The ideal reagent for transfection of siRNA

LullabyReagent HP

Lullaby ® - siRNA transfection reagent delivers the siRNA duplexes in a variety of cells with a very high

efficiency leading to exceptional knockdown effects with low doses of siRNA. Due to its exclusive properties,

Lullaby® is a gentle and robust reagent for gene silencing. Adopt this universal solution and wish your genes

Good Night, Bonne Nuit, Buenas Noches, Gute Nacht, , ....

RNA interference is a powerful technique to knockdown gene expression in cells and organisms. siRNA (small

interfering RNA), shRNA (small hairpin RNA) and dsRNA (double strand RNA) offer a convenient method to

study gene's function and constitute a promising approach for new therapeutic treatments. Short RNA duplexes

are extremely selective by interacting and inducing the degradation of their specific mRNA targets and thereby

inhibit the resulting protein production. Transfection reagents must efficiently deliver the siRNA into cells, with

minimal toxicity and without non-specific effects, to ensure you reliable results.

Lullaby , a sweet song for silencing your genes

GFP

Inhi

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0 24h 48h 72h 96h

Control0,1 nM1 nM10 nM20 nM

fig. 4: HeLa-GFP assayed in 24-well plate with Lullaby® and siRNA. GFP expression recorded in function of post-transfection time and siRNA concentration.

fig. 5: Various GFP-stably transfected cells treated with 3µL of Lullaby® and 10nM of siRNA in 24-well plates. Reagent HP used according to supplier's protocol. GFP expression monitored 72h post-transfection.

0

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LacZ 30000 50000 70000

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fig. 6: HeLa-GFP cells transfected in 24-well plate with 10nM of siRNA and 2µL of Lullaby®. GFP expression monitored 72h post-transfection.

Control Lullaby®

fig. 7: HEK293 cells treated with 3µL of Lullaby® and 20nM of siRNA targeting GAPDH gene. GAPDH expression monitored 48 to 72h post-transfection by immunocytochemistry. F I T C - a n t i - G A P D H antibody and DAPI detected by fluorescent microscopy.

0COS7 Hela CHO MDCK NIH3T3 Vero

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A549 293T Vero COS7

Control Lullaby Reagent HP SilenceMagfig. 8: Cells co-transfected in 24-well plates with 20nM of siRNA and 3µL of Lu l laby® or SilenceMag. Reagent HP used according m a n u f a c t u r e r ' s protocol. pGFP plasmid DNA complexed to 0.5µL of PolyMag transfected 4 hours later. GFP expression monitored 72h post-transfection.

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LacZ

Lulla

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GFP

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fig. 9: H441 (human lung adeno-carcinoma epithelial cell line) stably transfected with GFP assayed in 24-well plates using 2µL of Lullaby® and 10nM of siRNA. Others reagents used as described in supplier's protocol. GFP expression monitored 72h post-transfection.

High reliable and reproducible gene silencing

fig. 2: HeLa-GFP transfected in 24-well plate with 10nM of siRNA and 2µL of Lullaby®. Other reagents used according supplier's protocol. GFP expression monitored 72 hours post transfection.

fig. 3: MiaPaCa cells assayed in 6-well plate with 8µL of Lullaby® and 50nM siRNA targeting TAK1 (TGF-β activated kinase) or control (GFP). TAK1 expression monitored 72 hours post transfection by western blot using GAPDH as control. Courtesy of Dr Giroux, Inserm U624, Marseille, France.

As a ready-to-use and simple reagent, gene silencing experiment is quickly handled in a 3-steps procedure. Assay can be performed after a short incubation time: first significant levels of gene knockdown can be observed and analyzed 24 hours post-transfection.

Rapid and straightforward procedure Highly efficient

Effective with very low doses of siRNA

Minimize off-target effects

Applicable to a broad range of cell lines

Lullaby® main features

Serum compatible & Non toxic

Convenient for endogenous targeting and co-transfection

Usable with a large spectrum of cell culture conditions

Rapid and easy procedure

Because Lullaby® is a safe formulation; high cell viability is maintained in every experiment. Its little-to-no cytotoxicity level allows performing efficient co-transfections using a transfection reagent like DreamFect™ or PolyMag simultaneously.

Biodegradable

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Lulla

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Please visit www.ozbiosciences.com for more results

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