maleimide reagents
DESCRIPTION
Maleimide based reagents used in thiol chemistry reactivity used to study membrane topology and whole-cells and membrane systems.TRANSCRIPT
Year
2004
2006 Unknown N/A
2005 N/A N/A
2011 N/A N/A
1995 N/A N/A
2001 N/A N/A
2006 N/A N/A
2011 N/A N/A
2005 N/A N/A
2003 N/A N/A
2004 N/A N/A
Maleimide biotin (EZ-Link PEO maleimide biotin; ()-biotinyl-3-maleimidopropionamidyl-3,6-dioxaoctanediamine,MB)
sulfo-NHS-biotin (N-hydroxysulfosuccinimidobiotin)
525 Da Hydrophilic ER membrane permeable - microsomes
443 Da Negatively charged ER membrane permeable - microsomes
2002 N/A N/A
2010 Cell membrane impermeable Cell membrane impermeable
2010 N/A N/A
1998 N/A N/A
2013 N/A N/A
2012 N/A N/A
1999 N/A N/A
2011 N/A N/A
2014 N/A N/A
1996
2004
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EZ-Link sulfo-NHS-LC-biotin [sulfosuccinimidyl-6- (biotinamido) hexanoate]
N-(3-maleimidylpropionyl)biocytin
Maleimide-polyethyleneglycol (mPEG)
Sulfosuccinimidobiotin(SSB)
537 Da negatively charged ER membrane permbeable (selective modification) - whole cells
525 Da Hydrophilic ER membrane permeable - microsomes
5 kDA Hydrophilic ER membrane impermeable - whole cells
5 kDA Hydrophilic ER membrane impermeable - membrane vesicles
Cell membrane permeable
Cell membrane impermeable
Cell membrane permeable
ER membrane permeable - microsomes
Cell membrane permeable
ER membrane permeable - microsomes
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Relatively membrane impermeable - proteoliposomes
Bacterial membrane permeable
Cell membrane impermeable
Cell membrane impermeable
Bacterial membrane permeable
MTSEA MTSES
N/A N/A N/A N/A
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Maleimide neutravidin (MN)
N-ethylmaleimide (NEM)
60 kDA ER membrane impermeable - membrane vesicles
ER membrane impermeable - membrane vesicles
Cell membrane permeableViral membrane permeable
Cell membrane permeable
Quncher (blocking agent)
N/A N/A N/A N/A
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ER membrane permeable - membrane vesicles
Membrane impermeable - blocking
Cell membrane permeable
Cell membrane impermeable
Cell membrane permeable
N/A N/A N/A N/A
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Stilbenedisulfonate maleimide (4-acetamido-4 -′maleimidylstilbene-2,2 -disulfonic acid′
PEG-mal,methoxypolyethylene glycol maleimide 5000;
Maleimide-PEO2-biotin (MPB)
Methyl-PEO12-maleimide (MPM)
Cell membrane impermeable
Cell membrane impermeable
N/A N/A N/A N/A
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Cell membrane impermeable
Membrane permeable
Membrane impermeable - blocking
Membrane impermeable - microsomes
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5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB)
[2-(trimethylammonium)ethyl]methanethiosulfonate bromide(MTSET)
4-acetamido-4¢-maleimidylstilbene-2,2¢-disulfonic acid (AMS)
Biotin-PEG2-maleimide
Viral membrane impermeable
Cell membrane impermeable
Bacterial outer membrane permeable, inner membrane impermeable
ER membrane impermeable (blocking)
ER membrane impermeable (blocking)
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Membrane impermeable - proteoliposomes
Bacterial membrane impermeable (blocking)
Cell membrane impermeable
Bacterial membrane impermeable
Biotin maleimide (BM) Protein
N/A N/A
N/A N/A DP1 and Rtn4c/NogoC
N/A N/A
N/A N/A
N/A N/A Human P Glycprotein
N/A N/A SUR1
N/A N/A N/A
N/A N/A SecY Channel
N/A N/A
N/A N/A HPC-1/syntaxin 1A
N/A N/A Human Insig-1
(methyl-PEG12)3-PEG4-maleimide (PEGmal).
Stearoyl-CoA desaturase (SCD)
N/A N/A LacY
N/A N/A
Unknown N/A
N/A N/A Human P Glycprotein
N/A NBCe1 Protein
N/A N/A
N/A N/A
N/A N/A
N/A N/A DGAT2
Plasmalemmal dopamine transporter (DAT)
SA-I, type I signalanchor;SA-II, type II signal-anchor
Cell membrane permeable - whole cell
Polyisoprenyl-phosphate N-acetylaminosugar-1-phosphate transferase
Exofacial surface proteins
Cell surface glycoproteins
Paper
The Endoplasmic Reticulum Membrane Is Permeable to Small Molecules
A Class of Membrane Proteins Shaping the Tubular Endoplasmic Reticulum
Distinct effects of N-ethylmaleimide on formyl peptide- and cyclopiazonic acid-induced Ca2+ signals through thiol modification in neutrophils
Membrane Topology of a Cysteine-less Mutant of Human P-glycoprotein
Transmembrane Topology of the Sulfonylurea Receptor SUR1
Membrane Topology of Mouse Stearoyl-CoA Desaturase 1*
Membrane Topology of Human Insig-1
Inactivation of hantaviruses by N-ethylmaleimidepreserves virion integrity
Topology of transmembrane proteins by scanning cysteineaccessibility mutagenesis methodology
Preserving the membrane barrier for smallmolecules during bacterial protein translocation
Identification of the Carboxyl-Terminal Membrane-AnchoringRegion of HPC-1/Syntaxin 1A with the Substituted-CysteineAccessibilityMethod and Monoclonal Antibodies
Topology of polytopic membrane proteinsubdomains is dictated by membranephospholipid composition
Characterization of the highly conserved VFMGD motif in a bacterial polyisoprenyl-phosphate N-acetylaminosugar-1-phosphate transferase
Labile disulfide bonds are common at the leucocyte cell surface
On the Use of Thiol-modifying Agents to Determine Channel Topology
Membrane Topology of the H+-pyrophosphatase of Streptomyces coelicolor Determined by Cysteine-scanning Mutagenesis
Membrane Cholesterol Modulates the Outward FacingConformation of the Dopamine Transporter and AltersCocaine Binding*
Environmental Transition of Signal-Anchor Sequencesduring Membrane Insertion via the EndoplasmicReticulum Translocon
Molecular dissection of the human multidrugresistance P-glycoprotein
Topology of NBCe1 Protein Transmembrane Segment 1 andStructural Effect of Proximal Renal Tubular Acidosis (pRTA)S427L Mutation*
Redox Control of Exofacial Protein Thiols/Disulfides by ProteinDisulfide Isomeras
Characterization of the interaction of diacylglycerol acyltransferase-2with the endoplasmic reticulum and lipid droplets
Distinct effects of N-ethylmaleimide on formyl peptide- and cyclopiazonic acid-induced Ca2+ signals through thiol modification in neutrophils
Topology of polytopic membrane proteinsubdomains is dictated by membranephospholipid composition
Characterization of the highly conserved VFMGD motif in a bacterial polyisoprenyl-phosphate N-acetylaminosugar-1-phosphate transferase
Membrane Topology of the H+-pyrophosphatase of Streptomyces coelicolor Determined by Cysteine-scanning Mutagenesis