mandatory experiments on food

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Mandatory experiments on Food

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Test for reducing sugar

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Page 1: Mandatory experiments on Food

Mandatory experiments on Food

Page 2: Mandatory experiments on Food

Test for reducing sugar

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Test for fats

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Test for protein

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Test for starch

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16

Food Tests – Summary Chart

Test for Materials Method Positive ResultsStarch Iodine Solution Add iodine to the food sample A blue/black colourReducing sugar (Glucose)

Benedict's Solution Add to sample and warm gently A brick red colour

Protein Copper sulphate (CuSO4) and sodium hydroxide (NaOH)Biuret solution

Add some NaOH to the moist food. Then gently pour CuSO4 solution down the side of the tube

A violet colour

Fat Brown Paper Rub the food onto the paper, warm gently to dry and hold the paper up to the light

A translucent spot

Vitamin C DCPIP Add to moist food or fruit juice A change from blue to pink or colourless

Page 17: Mandatory experiments on Food

Experiment Questions

Food Tests

Page 18: Mandatory experiments on Food

Food Tests

For what is the Biuret test (copper sulphate and sodium hydroxide solutions) used in the

biology laboratory?

Test for protein

Page 19: Mandatory experiments on Food

Food Tests

For what is the Benedict’s (or Fehling’s) test used in the biology laboratory?

Test for reducing sugar

Page 20: Mandatory experiments on Food

Food TestsFor what purpose did you use brown paper or

Sudan III in food testing?

To test for Fat / Lipid / Oil

Page 21: Mandatory experiments on Food

Food Tests

What reagent or chemicals did you use to test for protein?

Biuret OR NaOH and CuSO4

Page 22: Mandatory experiments on Food

Food Tests

Is heat necessary in the test that you carried out to test for protein?

No

Page 23: Mandatory experiments on Food

Food Tests

What colour change indicates the presence of protein?

From blue to purple / violet

Page 24: Mandatory experiments on Food

Food Tests

Name the test or name the chemical used to test a solution for the presence of glucose

(reducing sugar).

Clinistix / Benedicts / Fehling’s / Copper

sulphate

Page 25: Mandatory experiments on Food

Food Tests

What colour change indicates a positive result for the presence of glucose?

(Clinistix) Orange to Blue or (others) Blue to Red

Page 26: Mandatory experiments on Food

Food Tests

Is heat necessary to test a solution for the presence of reducing sugar?

Yes

Page 27: Mandatory experiments on Food

Food Tests

For which purpose did you use Biuret solution or alkaline copper sulphate in food testing?

To test for (soluble) protein

Page 28: Mandatory experiments on Food

Food Tests

State a use for the Biuret test in the biology laboratory.

To detect presence of protein

Page 29: Mandatory experiments on Food

Food Tests

What is the initial colour of the reagent or chemicals used to test for protein?

Blue

Page 30: Mandatory experiments on Food

Food Tests

Was there a colour change in the control experiment when testing for protein?

No

Page 31: Mandatory experiments on Food

Food Tests

Name the test or give the chemicals used to test a sports drink for the presence of protein.

Biuret Test / Sodium Hydroxide & Copper sulphate

Page 32: Mandatory experiments on Food

Food Tests

If protein is present in a drink, what colour change would you expect to see? In your

answer give the initial and final colour of the test solution.

Blue to violet /purple / lilac

Page 33: Mandatory experiments on Food

Food Tests

Is heat necessary for the test for protein?

No

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Food Tests

For what purpose did you use Fehling’s solution or Benedict’s solution in the course of your

practical activities?

To check for Glucose / reducing sugar

Page 35: Mandatory experiments on Food

Food Tests

For which purpose did you use Brown paper or Sudan III in food testing?

To test for fat (or lipid or oil)

Page 36: Mandatory experiments on Food

Food Tests—Reducing sugar

(i) Name the reagent(s) used.(ii) State the initial colour of the reagent.

(i) Benedict’s/ Fehlings I and II, (ii) Blue

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Food Tests—Reducing sugar

State whether the test requires heat.

Yes

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Food Tests—Reducing sugar

What colour indicates a positive result?

Brick-red/orange

Page 39: Mandatory experiments on Food

Food Tests—Protein

(i) Name the reagent(s) used.(ii) State the initial colour of the reagent.

(i) Biuret solution / CuSO4 + NaOH (ii) Blue/ light blue

Page 40: Mandatory experiments on Food

Food Tests—Protein

State whether the test requires heat.

No

Page 41: Mandatory experiments on Food

Food Tests—Protein

What colour indicates a positive result?

Purple / violet

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Food Tests

(i) Name a test to show the presence of a reducing sugar (e.g. glucose).

(ii) Describe how you would carry out this test.

(i) Benedict’s/ Fehling’s (ii) Dissolve sample in water or put in test

tube / add reagent / heat / don’t boil / observe change or state result (brick-red-

orange precipitate)

Page 43: Mandatory experiments on Food

Food Tests

(i) Name a test for protein.(ii) Describe how you would carry out this test.

(i) Biuret test or named chemicals (ii) Add biuret reagent (or sodium hydroxide

and copper sulfate) / to sample / heat or shake / observe or record colour change

(purple-violet)

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Questions pg 13/14

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