manipulating molecules
DESCRIPTION
Manipulating molecules. Biot-111 Lecture 4. Types of molecules. Nucleic Acids Deoxyribonucleic acid (DNA) Chromosomal DNA Mitochondrial DNA (mtDNA, or less popularly, mDNA) Chloroplast DNA Ribonucleic acid (RNA) Ribosomal RNA (rRNA) Messenger RNA (mRNA) Transfer RNA (tRNA) - PowerPoint PPT PresentationTRANSCRIPT
Manipulating molecules
Biot-111Lecture 4
Types of molecules• Nucleic Acids
– Deoxyribonucleic acid (DNA)• Chromosomal DNA• Mitochondrial DNA (mtDNA, or less popularly, mDNA) • Chloroplast DNA
– Ribonucleic acid (RNA)• Ribosomal RNA (rRNA)• Messenger RNA (mRNA) • Transfer RNA (tRNA)
– Complementary DNA (cDNA) RNA-DNA Hybrid
• Proteins– Structural proteins
• E.g. Membrane proteins
– Functional proteins • E.g. Enzymes
» Helicases
» Transferases
» Proteases
» Nucleases
» Kinases
» Phosphatases etc.
Manipulating Nucleic Acids / cloning
Isolation and Purification of Nucleic Acid
Restriction analysis
Selection of suitable vector
Recombination
Insertion into a suitable host
Screening and selection of recombinants
The tools of recombinant DNA technology