Mark S. Nardone FAES/NIH

Director, Bio-Trac Program 301-496-8290

nardonem@mail.nih.gov One Cloister Court, Bldg 60, Rm 60

Bethesda, MD 20814-1460

What is Bio-Trac? • Hands-on Training Workshops in Targeted Areas of Molecular Biology • Taught by over 250 Active Researchers/Bench Scientists • Presented at a Graduate/Post-Graduate Level • Open to the Scientific Community • Average 3-5 Days in Length • Currently 56 Different Workshops Offered • Specialty Programs

Who Takes a Bio-Trac Workshop • NIH Researchers, Post Docs, Post Bacs, Fellows

– Lab Chiefs, Principle Investigators, Lab Technicians • FDA, USDA, USUHS, WRAIR, USAMRIID, CDC, US Patent Office, NIST • Academia

– Researchers, Teachers, Graduate & Undergraduate Students

• Private Industry – Presidents & V.P’s of Pharmaceuticals; Lab Chiefs – Bench Scientists – Technicians

• Over 16,000 Scientists Have Participated in a Bio-Trac Program

Where Are Bio-Trac Attendees Coming From

Why Take a Bio-Trac Workshop

• Effective Use of Time and Resources • Stay on Top of the Latest Developments in

Your Area of Research • Your Research Has Taken You into a New Field

of Study • Career Advancement • Budget Cuts – Having to do More With Less

Background Information

• Formally Established in the Fall of 1985 • Evolved Out of a Series of EEO Training

programs in the late 70s • Roland M. Nardone, Ph.D. – CUA

– Tissue Culture – Cell Line Authentication

• Drs. Lou Cohen, Earl Laurence and Freddie Brown (NIDDK)

Bio-Trac Workshops Specialty Training Programs

in Molecular Biology

Microscopy • Immunofluorescence and Confocal Microscopy • Digital Imaging in Microscopy

• Transgenic Technology

Microscopy

Microscopy

• Laser Capture Microdissection

Immunology

• Cellular Immunology • Immunocytochemistry • Immunochemistry and Monoclonal Antibody Production • Vaccines: Development and Evaluation of Efficacy

Proteomics & Genomics

• Proteomics: Principles and Methods • Clinical Proteomics and Biomarker Discovery • Genome Analysis • Protein-DNA Interactions: Gene Specific and

Epigenetic Regulation of Expression of the Mammalian Genome

• Protein Informatics • Epigenetics • Next Generation Sequencing • RNA Seq

Microarrays

• DNA Micro Arrays: Fabrication and Applications

• Advanced Microarray Analysis and Pathway Integration

GeneChip® CustomSeq™ Arrays

Cell Based Workshops • Animal and Human Cell Culture: Methods and

Applications • Cryobiology: Principles and Methods • Cell Line Identification and Authentication • Stem Cells

Stem Cell Based Workshops • Collaboration with the Center for

Regenerative Medicine Branch (CRM) at the NIH

• Stem Cells: Principles and Methods • Human Induced Pluripotent

Stem Cells (hiPSC); Differentiation to Neural Lineages

• Engineering with CRISPR, TALENs, and ZNFs • Making iPSCs From Blood • Making Cardiomyocytes from iPSCs • Stem Cell Industry Symposium

PCR Based Workshops

• PCR Methodology: Principles, Optimization and Applications

• Real Time and Quantitative PCR

Expression Analysis: siRNA & miRNA

• Small Interfering RNA (siRNA) and Functional Genomics

• miRNA: Tools and Technologies for the Quantitative and Functional Analysis in Mammalian Cells

In situ analysis of miRNA expression

Custom Designed Programs

• NIH Institutes (NCI, NIEHS, NINR, NIDDK, NINDS) • USDA • FDA • US Patent Office • Merk, UpJohn, Eli Lilly, Glaxo SmithKline,

Boehringer Mannheim…. • Colleges

“On The Road” Training Programs

• Custom Designed Training Workshops • Provide Training at Your Facility • Save Time, Travel and Money

Next Gen Sequencing Workshop at Colorado State University March 2014

Bio-Trac Laboratory Hosting

• Lab Safety Group • NCI Cancer Prevention Workshop • NIAMS – Stem Cell Workshops - Center for

Regenerative Medicine (NCRM) – Stemgent – StemCell

Bio-Trac Helpers Core Facilities

• Transgenic Core Facility • Angiogenesis Core Facility • Microarray Core Facility • Confocal Core Facility • LCM Core Facility

Private Industry

• Zeiss – Immunofluorescent Microscopes • Roche, ABI, Stratagene – Real Time PCR • BD - Flowcytometer • MMI, Zeiss Palm, Leica, MDS Arcturus – Laser

Capture Microdissection • Nikon, Zeiss – Microinjection Scopes

Specialty Programs • Minority Faculty Student Program • Provides biotechnology training to minority

students and faculty members • Participants from Historically Black Colleges

and Universities, Hispanic Serving Institutes and Indian Tribal Colleges or Universities

• Since the inception of the program in 1992, there have been 37 different MFSP workshops

• MFSP has provided over 480 students and 425 faculty members and touched over 90 institutions

• Participants gain insights and experience in relevant specialized areas of biotechnology through hands-on lab work and lectures

• Attendees also acquire information pertaining to research training opportunities at the NIH along with intramural and extramural funding opportunities

Summer Genetics Institute • Collaboration with the National Institute of Nursing Research

(NINR) • One month intensive research hands-on training program to

increase the research capability among graduate students and faculty

• Helps develop and expand the basis for clinical practice in genetics among clinicians

• Established in 2000 and has provided training to more than 230 nurses

• 130+ peer reviewed publications have come from SGI Participants

• 47 SGI graduates have received Federal funding for the research proposal they developed during the SGI program

Junior Scientist Training Program Established Fall 2010

• A quality training program at the NIH that bridges the gap between the laboratory skills acquired by biology graduates to the proficiency level necessary to be productive in a research laboratory setting

• 9 Month Program • 16 Laboratory Sessions • 23 Lecture Presentations • 6 Graduate Credits through the FAES

JSTP Results

• NIH Post Bacs, Post Docs, Fellows, Research Scientists

• Strengthen laboratory skills • Strengthen effectiveness as a researcher

• Recent College Graduates, 4th Year Biology Major • Strengthen graduate school application • Strengthen skill set for employment in the sciences • Strengthen internship application

JSTP Results Cont’d

• Acceptance into Graduate School • Acceptance into Internship Programs • Employment in the Sciences

What the Future Holds

• Expansion of JSTP in the USM System • New Courses

– iPSC in Cardiomyocytes – RNA-Seq – Super Resolution Microscopy

• New Laboratory Facilities (2016-17) – Two New Training Laboratories in Building 10

• Research Training for Medical Students – Working with Area Medical Schools

• Collaborations With Area Institutions

Thank you!