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Triple quadrupole

power andperformance,at a single quadrupole price

API 2000LC/MS/MS SYSTEM

API 2000LC/MS/MS SYSTEM

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Advanced MS/MS capabilities in a surprisingly small

footprint.The API 2000LC/MS/MS System from

AB SCIEX brings the power and accuracy of tandem mass

spectrometry to a wide range of applications. Designed

for maximum reliability and ease of use in demanding

high-throughput environments, this versatile, compact

instrument offers tremendous LC/MS/MS capability in the

space of a single quadrupole.

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The technology of choice for a widerange of applicationsTriple quadrupole MS/MS is one of the most sensitive and specific approaches for small-molecule

quantitation. The fragmentation capabilities of tandem mass spectrometry also provide critical

information for structural elucidation of unknown compounds.

Typical applications of the API 2000LC/MS/MS System:

Pharmaceutical

High-throughput pharmacokinetic screening

Metabolite identification

Impurity profiling

Food & beverage

Analysis of veterinary drug and pesticide residues

Detection and quantification of disease agents and

potential carcinogens

Environmental analysis

Identification of agrochemical metabolites in

environmental samples

Quantitative analysis of contaminants in water, soil,

and food

Forensic analysis

Identification of toxins

Quantitative analysis of forensic samples

Protein & peptide characterization

Precursor ion and neutral loss scanning detectionof target ions

With its exceptional sensitivity, automated methods development, and interchangeable

ion sources, the rugged API 2000System makes it possible to analyze hundreds

of samples per day. Patented atmospheric pressure ionization (API) technology and lab-proven engineering deliver superior performance for your most exacting analyses.

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A selection of easily interchangeable ion sources and flexible, intuitive software make it easy to

integrate the API 2000System into your applications and lab workflow.

Technology that keeps your labrunning at maximum productivity

Convenient plug-and-play ion sources

Rapid source change-over minimizes downtime and simplifies routine

maintenance. All temperature, gas, and electrical connections are

fully integrated into the source housing no extra lines to attach and

no lost time. Magnetic connections automatically detect hardware

changes and adjust the software accordingly.

Robust and trouble-free

Robust ion sources on the API 2000 System allow continuous analysis

of dirty samples that contain high concentrations of salt with no

degradation of performance.

Proven Curtain GasTechnology protects the interface and analyzer

from contamination. Routine maintenance and cleaning require

minimal tools, and all components are readily accessible for maximumuptime and productivity.

IQ1

STQ0 High Pressure CellCurtain Gas

Interface

Orifice Skimmer

Ion Source

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High sensitivity and reliability

High-pressure collisional focusing in Q0 maximizes ion transfer from

source to analyzer and ensures that even the most fragile compounds

reach the analyzer intact. The systems broad dynamic range

minimizes the need for sample concentration or dilution.0.0E+00

1.0E+06

2.0E+06

3.0E+06

0 2 0 4 0 6 0 8 0 1 0 0 1 2 0 1 4 0 1 6 0 1 8 0

Injection:25 L injections of terfenadine in Hanks salt buffer solutionHPLC:C8 HPLC (2.1 x 50 mm) with gradient elution of 500 L/minmobile phase directly (no split) into TurboIonSpraySource on theAPI 2000SystemNo. of injections:180 in 36 hoursQuantitation Method:External standardC.V.:6.4%

IQ2 IQ3

ExitQ1

LINAC

Collision Cell

Q2 Q3DF

Detector

Injection Number

AreaResponse

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Optimized for high throughput

Automated methods development and data processing make theAPI 2000System ideal for unattended, high-throughput,

quantitative analyses.

LINACCollision Cell

The patented LINAC Collision Cell ensures exceptional sensitivityand performance in all modes of MS/MS operation including

multiple reaction monitoring (MRM), neutral loss, and precursor ion

scan modes. By allowing you to reduce MRM dwell times without

compromising sensitivity, the LINAC Collision Cell significantly

increases the number of compounds you can measure per analysis.

High-sensitivity MRM methods developed for five candidate drugs and their

internal standard.

The API 2000System is ideal for fast determination of early pharmacokinetics of

candidate compounds.

1.0 2.0 3.0

20

40

60

80

100

120

140

160

180

200

220

240

260

280

300

320

340

360

380

400

420

Peak 1

Peak 2

Peak 3

Peak 4Peak 5

Internal Standard

120

100

80

60

40

20

0

0 1 2 3 4 5

Reducing dwell times has no effect on the sensitivity of the ABI 2000System, as

demonstrated here by repeat injections of selegiline.

10 msec

25 msec

50 msec

75 msec

100 msec

150 msec

200 msec

400 msec

0 .5 1.0 1 .5 2.0

1 0

2 0

3 0

4 0

5 0

6 0

7 0

8 0

9 0

1 0 0

Time, min

Candidate One

Candidate Two

Candidate Three

Sampling Time (Post Administration)

Candidate Four

Candidate Five

Time, min

PercentageIntensity

RelativeConcentrations

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For Research Use Only. Not for use in diagnostic procedures.

2010 AB SCIEX. The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners. AB SCIEXis being used under license.

0706710-01 09/2010

Headquarters

353 Hatch Drive | Foster City CA 94404 USA

Phone 650-638-5800

www.absciex.com

International Sales

For our office locations please call the division

headquarters or refer to our website at

www.absciex.com/offices

You invest in our technology.We invest in your success.As the world leader in mass spectrometry, AB SCIEX solutions are backed by the industrys

most extensive service and support organization. With a network of service professionals,

experienced compliance specialists, and over 150 PhD application scientists worldwide, we are

dedicated to supporting your technical needs and helping you get the most out of your

AB SCIEX systems.

AB SCIEX service professionals are recognized as the most highly qualified in the industry.

They are certified on our instrument platforms through a rigorous 4-step certification program,

with re-certification occurring every two years. This award-winning program helps to ensurethat you receive the most efficient, highest-quality, and most up-to-date service available for

AB SCIEX products and technology. Choose from flexible service plans and a variety of services

for the right level of support for your laboratorys needs and budget.

Our customer support network is available to provide expert assistance in the use and application

of AB SCIEX products through a comprehensive range of services, including application support,

technical service, and training.

Whether you access our service and support team by phone, email, on-site visits, or through

our innovative remote monitoring technology, you can be confident that the AB SCIEX

organization will be there for you.

For more information, visit www.absciex.com