Conforms to 93/112/EC and ISO 11014-1

Material Safety Data Sheet

BlotFreshTM Western Blot Stripping Reagent

100324

SL100324

CALL CHEMTREC:800.424.9300 3/21/2006

3/21/2006

Product name

Suppliernufacturer

Product No.

MSDS#Validation Date

Print DateIn Case of Emergency

Section 1. Chemical Product and Company IdentificationResponsible Name SignaGen Laboratories

Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications.

Eng

1) Ingredient Name #1 2-7 -- Xi R362) Ingredient Name #2 0.5-1 -- C R343) Ingredient Name #3 1-6 -- C R34

Ingredient Name EC NumberCAS No. R-Phrases

Substance/Preparation Preparation

% Symbol

:

Section 2. Composition, Information on Ingredients

Physical/chemical hazardsHuman health hazardsEnvironmental hazards Not applicable.

Irritating to eyes, respiratory system and skin.Not applicable.

Eye contact. Ingestion.

Emergency Overview

Medical Conditions Aggravated byOverexposure:

Repeated or prolonged exposure is not known to aggravate medical condition.

Not available.Overexposure /Signs/Symptoms

Section 3. Hazards Identification

Routes of Entry

Potential Acute Health Effects

Carcinogenic Effects Data

See Toxicological Information (section 11)

Avoid contact with eyes, skin and clothing. Avoid breathing vapor or mist. Keep container closed. Use with adequate ventilation.Wash thoroughly after handling.

Hazardous in case of skin contact (irritant), of eye contact (irritant), of inhalation (lung irritant). Slightly hazardous in case ofskin contact (corrosive, permeator), of eye contact (corrosive), of ingestion, of inhalation (lung corrosive).CARCINOGENIC EFFECTS: Classified 3 (Not classifiable for human.) by IARC [Ingredient Name #2].MUTAGENIC EFFECTS: Not available.TERATOGENIC EFFECTS: Not available.

Review the most current and approved institutional guideline, protocol, standard operating procedure(s) and MSDS(s)for the proper handling of institutional materials/equipment associated with the use of this product.WARNING!CONTAINS MATERIAL WHICH CAUSES DAMAGE TO THE FOLLOWING ORGANS: LUNGS,RESPIRATORY TRACT, SKIN, EYES, EYE, LENS OR CORNEA.MAY CAUSE RESPIRATORY TRACT, EYE AND SKIN IRRITATION.

Classification R36/37/38

United States

Europe

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Do NOT induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconsciousperson. Loosen tight clothing such as a collar, tie, belt or waistband. Get medical attention if symptoms appear.

Skin Contact

Ingestion

Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at least 15minutes. Cold water may be used. Get medical attention.

In case of contact, immediately flush skin with plenty of water. Cover the irritated skin with an emollient. Removecontaminated clothing and shoes. Cold water may be used.Wash clothing before reuse. Thoroughly clean shoes beforereuse. Get medical attention.

Hazardous in case of inhalation (lung irritant). Slightly hazardous in case of inhalation (lung corrosive).Slightly hazardous in case of ingestion.Sensitization of the product: Not available.Hazardous in case of skin contact (irritant). Slightly hazardous in case of skin contact (corrosive, permeator). Skininflammation is characterized by itching, scaling, reddening, or, occasionally, blistering.

Skin Contact

Hazardous in case of eye contact (irritant). Slightly hazardous in case of eye contact (corrosive).Eye Contact

Inhalation

Aggravating conditions Repeated or prolonged exposure is not known to aggravate medical condition.

First-Aid Measures

If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medicalattention.

Notes to Physician Not available.Not available.Protection of first-aiders

Ingestion

Effects and symptoms

Inhalation

Eye Contact

Section 4. First Aid Measures

Notice to Reader Get immediate medical attention.

Hazardous thermal (de)compositionproducts

Not applicable.

Non-flammable.

Not applicable.

Not applicable.

Not applicable.

Section 5. Fire Fighting Measures

Flammability of the Product

Flash Points

Fire Hazards in Presence of VariousSubstances

Fire Fighting Mediaand Instructions

Not applicable.Protective Clothing (Fire)

Absorb with an inert material and put the spilled material in an appropriate waste disposal. Neutralize the residue witha dilute solution of sodium carbonate. Finish cleaning by spreading water on the contaminated surface and allow toevacuate through the sanitary system. Be careful that the product is not present at a concentration level above TLV.Check TLV on the MSDS and with local authorities.

Section 6. Accidental Release Measures

Small Spill and Leak Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal container.If necessary: Neutralize the residue with a dilute solution of sodium carbonate. Finish cleaning by spreading wateron the contaminated surface and dispose of according to local and regional authority requirements.

Environmental Precautions andClean-up Methods

Personal precautions Splash goggles. Lab coat. Vapor respirator. Be sure to use an approved/certified respirator or equivalent. Gloves.

Keep container tightly closed. Keep container in a cool, well-ventilated area.

Avoid contact with eyes, skin, and clothing. Avoid breathing vapors or spray mists. Keep container closed. Use withadequate ventilation. Wash thoroughly after handling.

Section 7. Handling and Storage

Handling

Storage

Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications.

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Suitable / Not suitablePackaging materials

Use original container.

1) Ingredient Name #1 Not available.2) Ingredient Name #2 TWA: 8 CEIL: 8 (mg/m3) from BAuA [Germany] [1999] Inhalation3) Ingredient Name #3 Not available.

Germany

1) Ingredient Name #1 Not available.2) Ingredient Name #2 Not available.3) Ingredient Name #3 Not available.

Netherlands

1) Ingredient Name #1 Not available.2) Ingredient Name #2 STEL: 5 (ppm) from INRS [France] [1999] Inhalation3) Ingredient Name #3 Not available.

France

1) Ingredient Name #1 Not available.2) Ingredient Name #2 CEIL: 5 (ppm) from Arbeidstilsynet [Norway] [1996] Inhalation3) Ingredient Name #3 Not available.

Norway

1) Ingredient Name #1 Not available.2) Ingredient Name #2 CEIL: 5 (ppm) from Arbejdstilsynet [Denmark] [1996] Inhalation3) Ingredient Name #3 Not available.

Denmark

1) Ingredient Name #1 Not available.2) Ingredient Name #2 STEL: 5 (ppm) from AFS [Sweden] [1996] Inhalation3) Ingredient Name #3 Not available.

Sweden

1) Ingredient Name #1 Not available.2) Ingredient Name #3 Not available.

United States

Ingredient Name Occupational Exposure Limits

Engineering Controls Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below theirrespective threshold limit value.

Section 8. Exposure Controls/Personal Protection

Exposure Limit Values

Personal ProtectionEyesBody

Hands

Protective Clothing(Pictograms)

Gloves.Lab coat.Splash goggles.

Respiratory Respirator is not needed under normal and intended conditions of use, if exposures are kept below established limits.

The lowest known value is 100°C (212°F) (Milli-Q Water).

Liquid.

2.8 [Acidic.]

May start to solidify at -0.1°C (31.8°F) based on data for: Milli-Q Water.

Not applicable.

Not available.

Not available.

Clear (Light.)

Boiling/Condensation Point

Melting/Freezing Point

Physical State andAppearance

Molecular Weight

pH (1% Soln/Water)

Odor

Taste

Color

Section 9. Physical and Chemical Properties

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1 (Water = 1)

The highest known value is 0.62 (Air = 1) (Milli-Q Water).

The highest known value is 2.3 kPa (@ 20°C) (Milli-Q Water).

See solubility in water, methanol.

Easily soluble in cold water.Soluble in methanol.Partially soluble in hot water.Insoluble in diethyl ether.

0.36 (Milli-Q Water) compared to Butyl acetate.

Specific Gravity

Vapor Pressure

Vapor Density

Evaporation Rate

Dispersion Properties

Solubility

The product is stable.

Slightly reactive to reactive with oxidizing agents, organic materials, metals, alkalis.

Will not occur.

Not available.

Section 10. Stability and Reactivity

Stability and Reactivity

Conditions to avoid

Materials to avoid

Hazardous Polymerization

Hazardous DecompositionProducts

Not applicable.

Acute oral toxicity (LD50): 3500 mg/kg [Rat]. (Ingredient Name #3).Acute dermal toxicity (LD50): >3000 mg/kg [Rabbit]. (Ingredient Name #3).

Hazardous in case of eye contact (irritant), of inhalation (lung irritant).Slightly hazardous in case of ingestion, .

CARCINOGENIC EFFECTS: Classified 3 (Not classifiable for human.) by IARC [Ingredient Name #2].

Not available.

To the best of our knowledge, the toxicological properties of this product have not been thoroughly investigated. (IngredientName #3)

Not available.

Section 11. Toxicological Information

Toxicity to Animals

Chronic Effects on Humans

Other Toxic Effects on Humans

Special Remarks on Toxicity toAnimals

Special Remarks on ChronicEffects on Humans

Special Remarks on Other ToxicEffects on Humans

Ecotoxicity Not available.

Mobility

Germany water class

Not available.

Not available.

Not available.

Persistence/degradability

Bioaccumulative potential

VCI WGK: No products were found.

Section 12. Ecological Information

Waste must be disposed of in accordance with federal, state and local environmental control regulations.

Not available.

Waste Information

Waste Stream

Section 13. Disposal Considerations

Consult your local or regional authorities.

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Contact the supplier for all information regarding the proper transportation method for this material.

Section 14. Transport Information

U.S. Federal Regulations

Pennsylvania RTK: Ingredient Name #2: (environmental hazard, generic environmental hazard)Massachusetts RTK: Ingredient Name #2New Jersey: Ingredient Name #2

Irritating substance.Target organ effects.

HCS Classification

Section 15. Regulatory Information

California prop. 65: No products were found.

TSCA 8(b) inventory: Ingredient Name #1; Ingredient Name #2; Milli-Q WaterTSCA 8(d) H and S data reporting: Ingredient Name #2

Clean Water Act (CWA) 307: No products were found.

Clean Water Act (CWA) 311: Ingredient Name #2

Clean air act (CAA) 112 accidental release prevention: Ingredient Name #2

Clean air act (CAA) 112 regulated flammable substances: No products were found.

Clean air act (CAA) 112 regulated toxic substances: Ingredient Name #2

State Regulations

CEPA DSL: Ingredient Name #1; Ingredient Name #2; Milli-Q Water

Australia (NICNAS): Ingredient Name #1; Ingredient Name #2; Milli-Q Water

VCI WGK: No products were found.

Korea (TCCL): Ingredient Name #1; Ingredient Name #2; Milli-Q Water

Philippines (RA6969): Ingredient Name #1; Ingredient Name #2; Milli-Q Water

WHMIS (Canada) CLASS D-2A: Material causing other toxic effects (VERY TOXIC).

R36/37/38- Irritating to eyes, respiratory system and skin.DSCL (EEC)

International Regulations

Not available.EINECS

International Lists

SARA 302/304/311/312 extremely hazardous substances: Ingredient Name #2SARA 302/304 emergency planning and notification: Ingredient Name #2SARA 302/304/311/312 hazardous chemicals: Ingredient Name #2SARA 311/312 MSDS distribution - chemical inventory - hazard identification: Ingredient Name #2: immediate health hazard, delayed health hazard

R36/37/38- Irritating to eyes, respiratory system and skin.

S2- Keep out of the reach of children.S46- If swallowed, seek medical advice immediately and show this container or label.

Irritant

Label Requirements(Europe)

1

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Version 1

Date of Previous Issue No PreviousValidation

Not available.

Section 16. Other Information

References

Validated by SignaGen Administration on 3/21/2006 Verified by SignaGen Administration.

Printed 3/21/2006Notice to ReaderTo the best of our knowledge, the information contained herein is accurate. However, neither the above named supplier nor any of its subsidiaries assumes anyliability whatsoever for the accuracy or completeness of the information contained herein.Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution.Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist.

Hazardous MaterialInformation System

(U.S.A.)

101h

10

1

* National Fire ProtectionAssociation (U.S.A.)

Health

Specific Hazard

Reactivity

Fire HazardHealth

Fire Hazard

Reactivity

Personal Protection

History of Document Changes Any information changes since last document version are marked with a triangle symbol.Irritating to eyes.Causes burns.Causes burns.

Full text of R-Phrasesreferenced under headings 2 and 3:

Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications.