matrix spike recoveries - us epasummary matrix spike recoveries with method 1623.1 should be more...
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Matrix Spike Recoveries
Carrie Miller Cryptosporidium Lab Approval Program
Technical Support Center Standards and Risk Management Division Office of Ground Water and Drinking Water
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Matrix Spike vs Field Samples
• Procedure and limitations • Improved accuracy with Method 1623.1
in challenging water matrices • Observed matrix spike recovery with
Method 1623.1, n = 165
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Matrix Spike Procedure
• Mix sample continuously • Spike with pre-determined number of
Cryptosporidium oocysts • Perform Method 1623 or 1623.1 • Compare number detected with quantity added • Typically two matrix spikes for each source
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Matrix Spike Limitations
• Method variability • Matrix interference • Assumption
– spiked sample recovery = field sample recovery
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Method 1623 Variable in Reagent Water
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Blind Spike Average, Method 1623 ~50 Laboratories
Tennessee River Sediment Diatomaceous Earth
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MS Recoveries Round 1 Method 1623 n=3,335
November 15, 2012 U.S. Environmental Protection Agency
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Method 1623.1 Variable in Reagent Water
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Improved Accuracy in River Matrix
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Matrix Spike Results in Method Validations
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Improved Recovery in Challenging Matrices
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Multi-Laboratory Improvement with Challenging Matrix
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Observed Matrix Spike Recovery With Method 1623.1 n=165
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Summary
Matrix spike recoveries with Method 1623.1 should be more accurate than recoveries with Method
1623 in challenging source waters.
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