mau thuyet minh de tai.doc

7/30/2019 Mau thuyet minh de tai.doc

1/15

TRNG I HC NHA TRANGVin CNSH & Mi Trng

THUYT MINH TIKHOA HC V CNG NGH CP TRNG

1. TN TI:Ti u ha iu kin nui cyNannochloropsis Oculata trong mi

trng lng nhm thu lipid, nh hng sn xut nhin liu sinh hc

2. M S

3. LNH VC NGHIN CU

T nhinK

thut

Mitrng

Kinh t;XH-NV

NngLm-Ng ATL

Gio dc Y Dc S hutr tu

4. LOI HNH NGHINCU

Cbn

ngdng

TrinKhai

x

5. THI GIAN THC HINT thng 04 nm 2013 n thng 04 nm 2014

6. N V CH TR TITn n v: Vin Cng Ngh Sinh hc v Mi trngin thoi: 0583 832 075E-mail:a ch: S 2 Nguyn nh ChiuH v tn th trng n v ch tr: PGS.TS. Ng ng Ngha

7. CH NHIM TIH v tn: Phm Th MaiChc danh khoa hc: GV HDTHa ch c quan: Vin CNSH&MTin thoi c quan: 0583 832 075Di ng: 0945379349E-mail:[email protected]

Hc v: Thc sNm sinh: 1983a ch nh ring: Khu tp th H Nha Trangin thoi nh ring :Fax:

8. NHNG THNH VIN THAM GIA NGHIN CU TI

TT H v tnn v cng tc v

Lnh vc chuyn mnNi dung nghin cu c th

c giaoCh k

1. Nguyn CngMinh B mn CNSH, VinCNSH v Mi Trng Nghin cu ti u ha iukin trn mi trng lng nuicy chngN. oculata

Xc nh hm lng lipidtrong mu to nui cy.

9. N V PHI HP CHNHTn n v

trong v ngoi nc Ni dung phi hp nghin cuH v tn ngi i

din n v

x

x
mailto:[email protected]:[email protected]


2/15

10. TNG QUAN TNH HNH NGHIN CU THUC LNH VC CA TI TRONGV NGOI NC

10.1. Ngoi nc (phn tch, nh gi tnh hnh nghin cu thuc lnh vc ca ti trn th gii,lit k danh mc cc cng trnh nghin cu, ti liu c lin quan n ti c trch dn khi nhgi tng quan)

Vi to l cc sinh vt n bo, sinh trng bng quang t dng nh quang hp hoc d dng,hoc c hai (Liam Brennan, Philip Owende, 2009). Vi to c tc sinh trng nhanh, cho sinh khinhiu, din tch nui trng nh, tr lng du ln, khng cnh tranh t nng nghip, khng i himi trng nc sch, thm ch chng cn sinh trng trong mi trng nc thi nn thn thin vimi trng. Trung bnh 1kg sinh khi to th tiu th c 1,83kg CO2 (Attilio Converti, 2009).

Ty thuc vo loi m vi to cho ra nhiu loi sn phm khc nhau nh protein, lipit,cacbonhidrat v cc loi du phc hp khc (Banerjee et al., 2002; Metzger and Largeau, 2005). Do vi to c nui sinh khi nhm sn xut thuc, thc phm chc nng, cn, hidrogen (Yuan Kun Lee, 1997).

V c im sinh trng v cc li ch dinh dng, li ch kinh t m vi to mang li nn chngngy cng tr thnh i tng nghin cu nhiu trn th gii. u thp k 40 ca th k XX nhiuthc nghim nui cy to quy m ln c pht trin c (Albert and Wood, 1998).

u nhng nm 50 cc nh khoa hc vin Carnegie Washington c nhng ng gp lnkhi chng minh rng iu kin mi trng thay i c nh hng ti hm lng protein v hmlng lipit trong vi to Chlorella.

Nm 1957, Tamiya v cng s cng b cc cng trnh lin quan ti nui trng Chlorella, Nht Bn l mt trong nhng quc gia hng u sn xut v kinh doanh Chlorella di dng thc nb dng ( heath - food) v tc nhn kch thch sinh trng.

Nm 1960 ti Tip Khc cc nh khoa hc xy dng mt m hnh nui i tr Scenedesmustrn nn b c nghing 30 v to dng chy nh bm k thut Cascade. M hnh b ny c ngdng thnh cng ti Rupite, Bungart 1 a danh c sui nc nng ni ting sn xut i tr tolc Chlorella va Scenedesmus.

u nm 1970 Ryther J. H v cng s ti vin Hi dng hc Woods Hole pht hin mt hthng khp kn nui trng thc vt ni trn bin vi nc thi trn vi nc bin v thu to phcv nui tm c trc tip

Nm 1996 1997, tnh hnh nui vi to i tr trn nhiu nc trn th gii c thng k nhsau (Theo Yuan Kun Lee, 1997):

Khu vc To c sn xut Dintch (ha)

Sn lng(tn/nm)

ng dng

Nht Bn Chlorella -- 1100 v nhp943

Thuc b dng,thc n cho thu sn

Trung Quc Spirulina 199,6 2798 Dinh dng v xutkhu


3/15

i Loan Chlorella v Spirulina 24 1600 v 460 Xut khu

Triu Tin Chlorella v 1 s loito khc

2480 400 Cho ngi v thusn

n Spirulina 12,2 258,5 Cho ngi v ng

vtThi Lan Spirulina 2 170 Cho ngi v ng

vt

Vit Nam Spirulina 0,5 8 Xut khu

Innxia Chlorella -- 150 Xut khu

Hoa K Spirulina

Nitzschia

75

--

380 Cho ngi va ngvt

Mehico Sprulina -- 500 Cho ngi v ng

vtCuBa Spirulina -- 30 Cho ngi v ng

vt

c Dunaliella -- 40 Cho ngi v ngvt

Nm 2003 Hanhua Hu, Kunshan Gao nghin cu v sinh trng v thnh phn axit bo caNannochloropsis sp. vi cc ngun cacbon khc nhau. Kt qu cho thy mc sc kh 2% CO2kch thch sinh trng ca N. oculata ln nht.

Nm 2006 Hanhua Hu, Kunshan Gao tip tc nghin cu v sinh trng v thnh phn axit bot do vi cc nng CO2 khc nhau. Kt qu nghin cu cho thy hm lng CO2 l rt quantrng. Trong cc nng CO2 kho st th nng 2% CO2 chnh l nng im ti u nui cyN. oculata. Tuy nhin loi ny c tri qua qu trnh hun luyn thch nghi vi nng CO2 2%trc, sau nui cy trn mi trng c nng CO2 cao vn sinh trng kh tt. V vy c thkt hp s nui cy N. oculata ly lipid sn xut nhin liu sinh hc v hp th kh CO2 ci thin mitrng.

Nm 2009, Sarmidi Amin nghin cu sn xut kh v du sinh hc t vi to. Kt qu cho thy snphm t vi to c c tnh tng t nh cc sn phm t du thc vt v du c, c th thay th ccho du m.

Nm 2009, Attilio Converti v cng s nghin cu v nh hng ca nhit ti sinh trngv hm lng lipid ca N. oculata v Chlorella vulgaris. Kt qu nghin cu cho rng N. oculata

thch nghi trong mt ph nhit kh rng nhng li nhy cm khi sinh trng ti cc nhit khcnhau, nhit thch hp 20-250C. Nu nhit b h thp hoc b nng cao th sinh trng ca loito ny b hn ch.

Nm 2009, Sheng Yi Chiu v cng s nghin cu nh hng ca nng CO2 trong dngkh b sung vo mi trng nui cy ln sn lng sinh khi v s tch ly lipid N. oculata. Ktqu cho thy mc sc kh 2% CO2 kch thch sinh trng ca N. oculata cao nht.

Nm 2011, Subhash Nigam nghin cu nh hng ca nng nitrogen ti sinh trng vhm lng lipid thu c.. Kt qu cho thy khng ch thiu ht Nit m d tha qu nhiu Nit(dng NH4Cl) cng c ch sinh trng.


4/15

Nhin liu sinh hc c sn xut t nhiu ngun nguyn liu khc nhau nh: du ca mt s cyc du (ci du, c, u cc ro), m ng vt, du n thi, vi to (Felizardo et al., 2006;Kulkarmi and Dalai, 2006). Tuy nhin, vic nui trng vi to mt thi gian ngn hn, cho sinh khinhiu hn vi din tch nui trng nh hn v cho tr lng du ln hn. Mt s loi cho hm lnglipid cao, 30-50% trng lng kh.

Sn xut nhin liu sinh hc t vi to khng nh hng ti an ninh lng thc so vi sn xut tcc loi cy trng c du khc v thn thin vi mi trng hn dng nhin liu du m, hn navic s dng nhin liu sinh hc t vi to p ng cc yu cu hin hnh v nhin liu (Knothe,2006).

10.2. Trong nc (phn tch, nh gi tnh hnh nghin cu thuc lnh vc ca ti Vit Nam,lit k danh mc cc cng trnh nghin cu, ti liu c lin quan n ti c trch dn khi nhgi tng quan

Cc nghin cu trong nc v vi to cn hn ch. Mc d t thp nin 50 ca th k XX vi to c nui trng nhng sinh khi ch yu cung cp lm thc n chn nui, xut khu. Nhng nmgn y cng vi phong tro nghin cu sn xut nhin liu sinh hc ang pht trin nhiu nctrn th gii th Vit Nam cng c cc nghin cu v ngun nhin liu sinh hc nhng ch yu i

t m c, du n ph thi. s lng cc nghin cu v vi to nh hng cho sn xut nhin liu sinhhc cn hn ch.

Nm 1959, L Vn Cn v sau l nhiu ngi khc tin hnh nui bo Du- mt loi bocng sinh vi to Lam Anabaena c kh nng c nh Nit. Cc hp tc x min Bc tin hnhnui quy m ln ly sinh khi phn bn cho rung la, dng lm thc n cho gia sc (Lng VnLung, 1999).

Nm 1975, Vin sinh hc thuc vin Khoa hc Vit Nam cng tin hnh nghin cu qu trnhsinh l, sinh ha ca Spirulina v nghin cu s dng chng cho thc n chn nui (Trng VnLung, 2004)

T nm 1983, mt trm thc nghim sn xut thm canh v lin tc Spirulina platensis cxy dng Bnh Thun. Sn xut bn cng nghip loi to Lam ny cho 5 tn bt to Spirulinakh trong 9 thng, tng ng vi 7-10g cht kh/m 2/ngy hoc khong 20 tn/ha/nm (NguynHu Thc, 1987)

Nm 2005, mt s tnh pha Bc pht hin v phn lp c Labyrinthula c hm lng DHAcao (Hon Lan Anh v cng s,2005)

Nm 2006, Hong Th Ninh Hin v cng s nghin cu iu kin nui cy ti u chng vito binLabyrn thula sp. trn mi trng rn.

Nm 2009, Ng Hoi Thu, Hong Th Lan, ng Dim Hng nghin cu ti u ha iu kinnui cy trn mi trng lng chng Schizochytrium SP. PQ6 phn lp c ti huyn o Ph

Quc, tnh Kin Giang. Kt qu cho thy khi iu kin nui cy thay i nh hng ti hm lngcng nh thnh phn cc cht c trng vi to.

Nm 2010, Nguyn Th Minh Thanh v cng s cng b v sng lc cc loi vi to bin quangt dng c phn lp t vng bin ca Vit Nam nhm lm ngun nguyn liu cho sn xut nhinliu sinh hc.

Gn y, nm 2012 TS Nguyn Th Thanh Xun ging vin trng i hc Bch Khoa, ihc Nng c nhn gii thng LOral Unessco v s pht trin ca ph n trong khoahc vi ti : Nghin cu m hnh nui trng vi to to ngun sinh khi ng dng trong sn xutnhin liu sinh hc.


5/15

10.3. Danh mc cc cng trnh cng b thuc lnh vc ca ti ca ch nhim v nhngthnh vin tham gia nghin cu (h v tn tc gi; bi bo; n phm; cc yu t v xut bn)

Ting Anh:

1. Attilio Converti, 2009. Effect of temperature and nitrogen concentrationon the growth andlipid content of Nannochloropsis oculata and Chlororella vulagaris for nhin liu sinh hc

production, Chemical Engineering and Processing: Process Intersification, ELSEVIER, 48 1148-1151

2. Bligh E. G. and Dyer W. J., 1959: Can. J. Biochem. Physiol., 37: 911-917.

2. Hanhu Hu, Kunshan Gao, 2006. Response of growth and fatty acid composition ofNannochloropsis sp. to inviromental factor under elevanted CO2 concentration. Biotrchnol Lett, 28:987-992.

3. Hu H., Gao K., 2003. Optimization of growth and fatty acid composition of a unicellularmarine picoplankton, Nannochloropsis sp., with enriched carbon source. Biotechnol. Lett, 25: 421-425.

4. Subhasha Nigam, 2011. Effect of nitrogen on Grown and Lipid Concent of Chlorellapyrenoidosa, American Joural of Biochemistry and Biotechnology, vol 7, N03 124-129.

5. Zhu CJ, L YK, Chao TM, 1997. Efect of temperature and growth phase on lipid andbiochemical composistion of Isochrysis galbana TK1. J. Appl. Phycol, 9: 451-457.

6. http://www.chlorellavulgaris.com/

7. http:/www.oilgae.com

Ting Vit

1. ng Dim Hng, Hong Minh Hin, Nguyn nh Hng, Hong S Nam, Hong LanAnh, Ng Hoi Thu, inh Khnh Chi, 2007: Tp ch Khoa hc v Cng ngh, 45 (IB): 144-153.

2. ng Kim Bnh, Hong Phc Hin, 1999. Cng ngh sinh hc vi to, NXB Nng nghipH Ni

3. Nguyn Th Minh Thanh, Ng Th Hoi Thu, Hong Th Lan Anh, inh Th Thu Hng,ng Dim Hng, 2010: Tp ch Khoa hc v Cng ngh, 48 (4A): 320-325.

4. V Vn V, Nguyn Mng Hng, 2005. Cng ngh sinh hc, tp hai, NXB Gio Dc.

11. TNH CP THIT CA TI

Cho ti nay, trong nc c nhiu nghin cu v nui vi to ly sinh khi nhng ch yu tptrung vo cc mc ch: lm thc n cho lun trng ca cc n v nui trng thy sn, lm thc n

cho vt nui, xut khu, lm thc n b dng cho ngi v lm m phm.Nghin cu nui vi tonhm thu lipid lm nguyn liu sn xut nhin liu sinh hc (NLSH) cn hn ch.

Sn xut NLSH ang c c th gii quan tm. Chnh ph Vit Nam k n 117 pht trinNLSH mt dng nng lng mi ti to c thay th mt phn NLHT truyn thng, gp phnm bo an ning nng lng v bo v mi trng.

Ngun sn xut NLSH c th ly t m ng vt, du n thi, du ca cc cy c du. Khi sdng nhng nguyn liu ny gp phi cc hn ch sau:

- M ng vt v du n thi: khng ch ng c ngun nguyn liu, hiu sut sn xut
http://www.chlorellavulgaris.com/http://www.chlorellavulgaris.com/


6/15

thp do cha nhiu axit bo t do.

- Du ca mt s loi cy c du: thi gian trng lu, cnh tranh t trng vi cy nngnghip nh hng ti an ninh lng thc, gi thnh cao.

Nui cy vi to lm ngun cung cp nguyn liu cho sn xut NLSH l gii php tt nht. Trnth gii c rt nhiu nghin cu v vn ny vi nhiu cch tip cn khc nhau. Nui vi to

nhm thu lipid sn xut NLSH c cc u im sau:- Sn xut thu sinh khi ln trong thi gian ngn nn tit kim chi ph.

- Ch ng v ngun nguyn liu do tc sinh trng ca vi to nhanh.

- Khng i hi din tch t trng nn khng nh hng ti ngnh nng, lm nghip.

T nhng l do trn v qua nhiu ti liu nghin cu, chng ti thy nghin cu nui trng vi totrong mi trng sa i nhm mc ch thu lipid l hon ton kh thi.

Trong cc loi vi to bin th N. oculata l mt trong nhng loi vi to cho hm lng lipit cao(khong 22,7 29,7%). Mc d hm lng lipid ca N. oculata thp hn mt s loi nh Chlorellaemersonio, Chlorella sp.,Nannochloris sp.,. NhngN. oculata theo phng php t dng c scsinh sn cao, lng sinh khi trong ngy ln (Teresa M. Mata, Antnio A. Martins, Nidia. S, 2009 vAttilio, 2009). Hn na, nng sut lipid ca N. oculata c th thay i theo iu kin nui cy(Sukenik A., Carmeli Y., 1989). iu ny c ngha l N. oculata ang l mt tim nng v hmlng lipid nu tm c iu kin nui cy ph hp.

Hin nay Vit Nam cha c nghin cu no i su vo nghin cu ti u ha cc iu kin nuicy vi to bin nhm thu lipit cung cp nguyn liu cho sn xut NLSH, c bit loi c tim nngnhN. oculata.

V vy chng ti tin hnh nghin cu ti: Ti u ha iu kin nui cyNannochloropsisOculata trong mi trng lng nhm thu lipid, nh hng sn xut nhin liu sinh hc. Thnh

cng ca ti s m ra mt trin vng ln v nui cy vi to ni chung v nui cy vi to N.oculata ni ring vi quy m ln nhm cung cp nguyn liu cho ngnh sn xut NLSH.

12. MC TIU TI- Ti u ha iu kin nui sinh khi vi toNannochloropsis oculata c hm lng lipid cao

nhm nh hng sn xut nhin liu sinh hc- Xc nh hm lng lipid trongN. oculata ng vi tng iu kin nghin cu- Tch chit lipid t sinh khi vi to

13. I TNG, PHM VI NGHIN CU

13.1. i tng nghin cu:- Chng vi toNannochloropsis oculata ging thun c mua t vin cng ngh sinh hc H Ni

- V tr phn loi caN. oculata:

Ngnh Heterokontophyta, Hocck, Mann vaf Jahn, 1985

Lp Eustigmtophyceae Hibberd, 1981

B Eustigmatales Hibberd, 1981

H Monopsidaceae Hibberd, 1981


7/15

Ging Nannochloropsis Hibberd, 1981

Loi N. Oculata Hibberd, 1981

13.2. Phm vi nghin cu:

- Ti u ha mt s iu kin nh hng ti sinh trng ca N. Oculata : nhit , mn, ngunNit, iu kin chiu sng.

- Xc nh hm lng lipit tng ng vi mi iu kin nui cy: phng php Soxlet

- Nui cy vi toNannochloropsis oculata trong iu kin ti u nghin cu c

- Tch chit lipit t sinh khi thu c: phng php Bligh v Dyer

Cc th nghim c tin hnh ti vin Cng Ngh Sinh hc & Mi trng, Trng i hc NhaTrang.

14. CCH TIP CN, PHNG PHP NGHIN CU

14.1. Cch tip cn:

- Nui cy vi toNannochloropsis Oculata trn cc iu kin dinh dng khc nhau. La chniu kin ti u cho sinh khi ln nht vi hm lng lipid cao nht.

- a ra iu kin ti u trn mi trng lng nui Nannochloropsis Oculata vi mc ch thulipid.

14.2. Phng php nghin cu:

Ni dung 1:Ti u ha mt s iu kin nui cyN. Oculata vi mc ch thu lipid

a. B tr th nghim

Nng sut lipid c tnh l khi lng lipid c sn xut ra trn mi n v th tch canhtrng vi to mi ngy, ph thuc vo tc sinh trng ca to mi ngy v hm lng lipid ctrong vi to. Hm lng lipit trong t bo li ph thuc vo cc yu t nh: ngun Nit, nhit , mn... kho st cc yu t ny chng ti b tr th nghim nh sau:

Cc l nui cy c nghin cu c lp, mi l gm 5 bnh tam gic dung tch 1,5l c b trnh s di y (hnh 1). Cc l khc nhau v yu t kho st. C th nh sau:

- L 1: Kho st v nhit . Nhit tng ng trong cc bnh nui l: 18, 20, 25, 28, 300C.

- L 2: Kho st v mn. Nng kho st trong 5 bnh tng ng t: 0; 1%; 2%; 2,5%; 3%;4%

- L 3: Kho st v cng chiu sng 2000, 2500, 3000, 3500, 4000, 4500 (lux).

- L 4: Kho st v ngun Nit. ngun Nit kho st trong 5 bnh tng ng: (NH4) 2(SO)4,NH4NO3, NaNO3, ure, N-P-K


8/15

Hnh 1. S b tr th nghim kho st cc yu t nh hng ti tc sinh trngv kh nng lu tr lipid ca vi toN. oculata

b. phng php o quang: nh gi tc sinh trng ca vi to bng phng php o quang:da vo hp th nh sng ca Chlorophyll trong t bo. Cc sc t ny hp th ch yu cc bcsng 420nm v 650nm. Tin hnh o mi ngy 1 ln. Sinh khi s c thu hoch khi sinh trngca vi to bt u i vo pha cn bng (2 ngy o mt quang khng thay i).

Ni dung 2: Thu sinh khi v xc nh hm lng lipid

a. thu sinh khi: Sau khi xc nh c thi im thu sinh khi bng phng php o quang tinhnh thu sinh khi bng ly tm hoc dng cht kt ta phn nhm. Sinh khi c ra li vi ncct 3 ln ri em sy kh 800C nghin thu c sinh khi kh. Phn nc lc c ti sdng cho cc nui cy tip sau.

b. Xc nh hm lng lipid theo phng php Soxlet:

- Vic xc nh hm lng lipid s c tin hnh i vi mi bnh nui cy trong mi l. Ktqu c ghi nhn c lp phn tch v so snh.

- Cc bc tin hnh: cn chnh xc 5g mu sy n khi lng khng i 1050C chuynmu vo ti giy c sy n khi lng khng i cn li xc nh khi lng ca ti vmu t gi mu vo bnh chit chit t 6-8h vi dung mi chit l ete etanol ly gi mu rav cho bay hi ht dung mi sy kh n khi lng khng i.

Bnh 1 Bnh 5Bnh 4Bnh 3Bnh 2

Lc 200v/p

Ly mi bnh 1ml dch tin hnh o quang xc nhtc sinh trng (mi ngy o 1 ln)

Thu sinh kh i

Xc nh hm lng lipit bng Soxlet


9/15

- Tnh kt qu: %100).(

C

BAX

=

Trong : A: Khi lng nguyn liu trc khi chit rt lipid

B: Khi lng nguyn liu sau khi chit rt lipid

C: Khi lng sinh khi kh ly xc nh

Ni dung 3: Tch chit lipid t sinh khi theo phng php Bligh v Dyer

- Vic tch chit lipid c tin hnh vi ton b sinh khi thu c

- Cc bc tin hnh: Sau khi thu sinh khi kh, tin hnh chit lipid theo s sau

Ni dung 4: NuiN. oculata theo cc thng s ti u t cc ni dung trn

T cc kt qu nghin cu trn, b tr th nghim nui cyN. Oculata theo cc thng s ti u.Tin hnh thu sinh khi v tch chit lipid. T lng lipid tch chit c nh gi hiu sut ca tonb qu trnh tch chit.

Ngm bt to kh vi h n hp dungmi (chloroform:methanol=2:1)

Dchchit

Ph u chit

Thu hn hp lipid v dung mipha di

B c chi t li vi chloroform2-3 ln, thu dch chit

NaCl 0,9%

Loi dung mi trong b n nhit600C Lipid

Dung mi Ti s dng

nhit phng, 12h


10/15

15. NI DUNG NGHIN CU V TIN THC HIN15.1. Ni dung nghin cu (trnh by di dng cng nghin cu chi tit)

- Nghin cu ti u ha iu kin nui cyN. oculata- Thu sinh khi to v xc nh hm lng lipid trong to ng vi iu kin nui cy- Tch chit lipid t sinh khi theo phng php Bligh v Dyer

- Nghin cu nuiNannochloropsis oculata trong iu kin ti u nghin cu15.2. Tin thc hin

STTCc ni dung, cng vic

Thc hinSn phm

Thigian(bt u-kt

thc)

Ngi thchin

1 Nghin cu ti u ha iu kinnui cy vi toNannochloropsisOculata nhm thu lipid

Bo co khoa hc vnghin cu ti u haiu kin nui cy vi toNannochloropsisOculata nhm thu lipid

05-10/2013 Phm Th MaiNguyn CngMinh

2 Thu sinh khi toN. oculata vxc nh hm lng lipid trongtoN. oculata ng vi iu kinnui cy

- Sinh khi to thu c- Bo co khoa hc vvic xc nh hm lnglipid trong toN.oculata ng vi tngiu kin nui cy

11/2013-12/2013 Phm Th MaiNguyn CngMinh

3 Tch chit lipid t sinh khi theophng php Bligh v Dyer

- Bo co khoa hc vvic tch chit lipid tsinh khi theo phngphp Bligh v Dyer- Quy trnh tch chit

lipid vi hiu sut cao

01/2014-02/2014

Phm Th MaiNguyn CngMinh

4 Nghin cu nuiNannochloropsis oculata trongiu kin ti u nghin cu

- Sinh khi to thu c- Quy trnh nui cyN.oculata vi cc thng sti u

03/2014 Phm Th MaiNguyn CngMinh

5 Vit bo co tng kt ti Bo co tng kt ti 4/2013 Phm Th Mai


11/15

16. SN PHM

16.1. Sn phm khoa hc

Sch chuyn kho Bi bo ng tp ch nc ngoiSch tham kho Bi bo ng tp ch trong ncGio trnh Bi ng k yu hi ngh, hi tho

16.2. Sn phm o toSinh vin Nghin cu sinh Cao hc

16.3. Sn phm ng dng

Mu Vt liu Thit b my mcGing cy trng Ging vt nui Qui trnh cng ngh xTiu chun Qui phm S , bn thit kTi liu d bo n Lun chng kinh tPhng php Chng trnh my tnh Bn kin ngh

Dy chuyn cng ngh Bo co phn tch Bn quy hoch16.4. Cc sn phm khc: ti liu tham kho cho sinh vin, hc vin cao hc v cc i tng khc

c nghin cu v vi to v nhin liu sinh hc.

16.5. Tn sn phm, s lng v yu cu khoa hc i vi sn phmSTT Tn sn phm S lng Yu cu khoa hc

1 Quy trnh nui cyN. oculata vi ccthng s ti u

01 Quy trnh n nh v p dng hiu qu

2 Quy trnh tch chit lipid vi hiu sut cao 01 Quy trnh n nh v p dng c hiuqu

3 Lipid tch c 20ml

4 Bi bo khoa hc 01 c chp nhn cho ng trn Tp chchuyn ngnh

17. HIU QU (gio dc v o to, kinh t - x hi)Ngun nguyn liu sn xut NLSH ang c rt quan tm ca rt nhiu nc trn th gii trong c Vit

Nam. Nh trnh by trn, nu i t ngun nguyn liu truyn thng nh m ng vt, du n thi, du canhng cy c du, gp phi nhiu kh khn: phn ng chuyn v este kh thc hin, hiu sut thp, gi thnhcao, mt thi gian, nh hng ti an ninh lng thc, Cho ti nay, nui vi to ly sinh khi, thu lipid lmnguyn liu cho sn xut NLSH l gii php tt nht

Nui vi to ngoi mc ch thu nhn lipid lm nguyn liu cho sn xut NLSH, sinh khi to cn phc vvo nhiu mc ch khc: lm thc n cho lun trng (rt c ngha kinh t i vi cc c s nui trng thysn), lm thc n cho vt nui ( ngha kinh t i vi cc c s chn nui), chit mt s thnh phn c trongvi to nhm ch bin thc n b dng cho ngi (c bit c ngha i vi nhng ngi n chay), sn xutthc phm chc nng, lm m phm,

V nhng l do m ti u ha iu kin nui cy vi to v cng c ngha i vi nhiu lnh vc khcnhau.

Kt qu nghin cu ca ti ngoi nhng li ch trn cn cung cp thm t liu ging dy, hc tp v thamkho cho nhiu ging vin v sinh vin trong v ngoi trng.18. PHNG THC CHUYN GIAO KT QU NGHIN CU V A CH NG DNG

Kt qu nghin cu ca ti gp phn vo gii quyt vn nguyn liu sn xut NLSH ang rt cquan tm. V vy n s c ng dng cho cc nghin cu tip theo vi quy m ln hn, nhm tin ti xy dngm hnh nui cy vi to thu lipid cung cp cho cc n v sn xut NLSH. Ngoi ra kt qu nghin cu ca ticng c ng dng cho cc nghin cu nui sinh khi vi to vi nhiu mc ch khc.

x

x


12/15

19. KINH PH THC HIN TI V NGUN KINH PH

Tng kinh ph: 47,000,000 ngTrong :

Ngn sch Nh nc: 47.000.000 ng Cc ngun kinh ph khc:

Nhu cu kinh ph tng nm:- Nm 2013: 47.000.000 ng

D tr kinh ph theo cc mc chi (ph hp vi ni dung nghin cu):n v tnh: triu ng

Stt Khon chi, ni dung chi Thigianthchin

Tngkinhph

Ngun kinh ph Ghi ch

Kinhph tNSNN

Ccngunkhc

I Chi cng lao ng tham gia trc t ip

thc hin ti

8,5 8,5

II Chi mua nguyn, nhin, vt liu, thit b 36,0 36,0

III Chi khc 5,0 5,0- Vn phng phm, in bo co tng kt 0,3 0,3- Nghim thu ti 1,1 1,1- Ph cp ch nhim ti 3,6 3,6

Tng cng 49,5 49,5

Ngy thng nm 2013n v ch tr

Ngy thng nm 2013Ch nhim ti

Phm Th MaiNgy thng nm 2013

C quan ch qun duytHIU TRNG


13/15

Ph lc: D TON KINH PH CHI CHO TI

I- D ton chung cc khon chi

STT Ni dung cc khon chiKinh ph(triu ng)

Tng s T NSNN Khc Ghi ch1 Cng lao ng trc tip thc hin ti 8.5

2 Mua nguyn, nhin, vt liu, thit b 293 Sa cha, mua sm ti sn c nh 74 Chi khc 2.5

Tng s 47

II- Din gii cc khon chi

Khon 1: Cng lao ng trc tip thc hin ti

(n v tnh: triu ng)

STT Ni dungTng

kinh ph

Ngun vn

NSNN Khc Ghich1 Nhn ging v bo qun ging 12 Nghin cu nh hng ca nhit ti tc sinh

trng v kh nng tr lipid ca vi to0.8

3 Nghin cu nh hng ca nh sng ti tc sinhtrng v kh nng tr lipid ca vi to

0.8

4 Nghin cu nh hng ca nng CO2 ti tc sinh trng v kh nng tr lipid ca vi to

0.8

5 Nghin cu nh hng ca ngun Nito khc nhau titc sinh trng v kh nng tr lipid ca vi to

0.8

6 Nghin cu nh hng ca mn ti tc sinhtrng v kh nng tr lipid ca vi to

0.8

7 Thu sinh khi 0.88 Kho st hm lng lipit bng soxlet 0.89 Thu lipit 0.810 Kho st quy trnh nui cy v thu lipid t nhng

thng s ti u trn1.5

11 Vit thuyt minh tng kt ti 0.4Cng 1 8.5

Khon 2: Nguyn, nhin, vt liu, thit b

(n v tnh: triu ng)

TT Ni dung VT S lng n giThnh

tin

Ngun vn

NSNN KhcGhich

1 NaCl chai 0.5 0.5

2 NaOH tinh th chai 0.5 0.5


14/15

3 CuSO4 chai 0.5 0.5

4 FeCl3 chai 0.5 0.5

5 KH2PO4 chai 0.7 0.7

6 KNO3 chai 0.5 0.5

7 KMnO4 0,1N ng 0.5 0.5

8 Glucose chai 0.4 0.49 Sacarose chai 0.4 0.4

10 Lactose chai 0.4 0.4

11 Mantose chai 0.4 0.4

12 ure chai 1 1

13 Ete etylic chai 3 3

14 NH4Cl chai 0.5 0.5

15 Phenolphtalein chai 0.5 0.5

16 FeSO4 chai 0.5 0.5

17 HCHO lt 3 318 HCl 0,1N ng 0.5 0.5

19 HCl chai 0.2 0.2

20 giy o pH tp 0.2 0.2

21 giy lc hp 1.5 1.5

22 cn Lt 1.5 1.5

23 Agar ti 0.8 0.8

24 MnSO4 chai 1 1

25 Glycine g 1 1

26 Cloroform L 3.5 3.527 methanol L 1.2 1.2

28 N-P-K kg 0.8 0.8

29 NaNO3 chai 1 1

30 NH4NO3 chai 1 1

31 (NH4)2(SO)4 chai 1 132 Bnh tam gic

1,5l nui vito

2 2

33 H thngchiu sn

4 4

34 H thng sckh

1 1

Cng (2) 36 36


15/15

Khon 3: Chi khc

(n v tnh: triu ng)

TT Ni dungKinhph

Ngun vnNSNN Khc Ghi ch

1 Vn phng phm, in n bo co tng kt 0,3

2 Th lao trch nhim ca ch nhim ti (300.000ng/thng x 12 thng)

3,6 3,6

5 Nghim thu 1,1 1,1Cng (3) 5,0 5,0

n v ch tr Ch nhim ti

Phm Th MaiPhng K hoch - Ti chnh Phng Khoa hc Cng ngh

mau thuyet minh de tai.doc

Documents