mechanical properties of collagen molecule
TRANSCRIPT
Mechanical Properties of Proteins
Swapnil Khachane
Take Away
1. What are proteins
2. Necessity of the study
3. Sarcomere and Titin
4. Go-like Model
5.Results
6.Summary
Purpose of the Study
• Diseases
• Injury/Trauma
• Future Inventions
Titin
From. Cieplak 2002Figure 1. Structure of Titin
Titin
Figure 2. Structure of TitinFrom. Tshovorebova 2005
Sarcomere in a Striated Muscle
From. Cieplak 2002Figure 3. Sarcomere
Go Like Model
From. Buehler 2008
• Cα Atoms
• Lennard Jones Potential
• Equation of Motion
612
ij 24 ij
ij
ij
ij
rrE
randFFcrrm
Results from the model
Figure 4. Force Displacement Curve for 1titFrom. Cieplak 2005
Experimental Results
Figure 5. Force Displacement Curve for 3tit
From. Tshovorebova 2005
Experimental Results
Figure 6. Force Displacement Curve for 3tit
From. Tshovorebova 2005
References•Sulkowska et al., Predicting the order in which contacts are broken during single molecule protein stretching experiments, Wiley-Liss Inc, 2007.•Cieplak et al. , Folding and Stretching in a Go-like Model of Titin.•Cieplak et al,. Stretching of proteins in the entropic limit. Phys Rev e 2004; 69: 011912.•Erickson HP. Protein biophysics - stretching single protein molecules: titin is a weird spring. Science 1997; 276: 1090-1092.•Tskhovrebova et al, Elasticity and unfolding of single molecules of the giant muscle protein titin. Nature 1997; 387: 308-312.•Dietz H,Berkemeier F,Bertz M,Rief M. Anisotropic deformation response of single protein molecules. Proc Natl Acad Sci USA 2006; 103: 12724-12728.•West et al, Mechanical Resistance of Proteins Explained Using Simple Molecular Models, Biophysical Journal, Volume 90, Issue 1, 287-297, 1 January 2006.•Ceiplak et al, Pulling single bacteriorhodopsin out of a membrane: Comparison of simulation and experiment, Biochimica et Biophysica Acta (BBA) - Biomembranes•Volume 1758, Issue 4, April 2006, Pages 537-544.•Kil Ho Eom, The Mechanical Modeling of Proteins.•Yoon et al, Mesoscopic Model for Mechanical Characterization of Biological Protein Materials, Nano-Bio Research Center, Korea Institute of Science & Technology (KIST), Seoul 136-791, Republic of Korea.
Thank You