mechanisms of evolution-ii
TRANSCRIPT
Mechanisms of Evolution II
• Overproduction & competition
• Phenotypic variation
• Some variation is heritable
• Differential survival & reproduction
*Leads to changes in allele frequencies over time
Natural Selection Reminder
The Equilibrium Population:Assumptions of Hardy-Weinberg
• No mutation (no new genetic variation)
• No genetic drift (infinitely large population)
• No migration (no individuals entering or leaving the pop)
• No selection (genotypes have equal fitness)
• Random mating (dealing with a single population)
Example of Hardy-Weinbergp2 +2pq + q2 = 1
The Equilibrium Population:Assumptions of Hardy-Weinberg
• No mutation (no new genetic variation)
• No genetic drift (infinitely large population)
• No migration (no individuals entering or leaving the pop)
• No selection (genotypes have equal fitness)
• Random mating (dealing with a single population)
Recall: DNA replicates at every cell division => Opportunity for error and repair (mutation)
Recall: DNA replicates at every cell division => Opportunity for error and repair (mutation)
Recall: DNA replicates at every cell division => Opportunity for error & repair (mutation)
mismatchmust berepaired
if C =>T,a mutation
if A => G,no mutation
Mutation• alterations of the base DNA sequence
• ultimate source of all genetic variation• many types of mutation (e.g. point, chromosomal)• often reduce fitness (deleterious), but can be beneficial or neutral too
• weak force in changing allele frequencies over time (evolution)
• many genes per genome (~30,000 in humans), so odds of hitting any particular one is very low per
generation
The genetic code is degenerate => many mutations are silent
GUUGUCGUAGUG
Valine, so 3rd position mutations don’t affect protein sequence
Recall: DNA => mRNA => protein => => => phenotype
If mutation changes protein sequence, it may affect phenotype (e.g. Sickle cell anemia)
Recall: DNA => mRNA => protein => => => phenotype
single amino acid change
The Equilibrium Population:Assumptions of Hardy-Weinberg
• No mutation (no new genetic variation)
• No genetic drift (infinitely large population)
• No migration (no individuals entering or leaving the pop)
• No selection (genotypes have equal fitness)
• Random mating (dealing with a single population)
Genetic drift: an extreme example• Imagine a population of only 1 M and 1 F per generation (N = 2)
• Start with 2 heterozygotes (Aa), p & q = 0.5
• Simulate allele formation & random mating with coin flip
1.0
.50
.75
.25
alle
le fr
eq.
0 1 2 3 4 5 6 7 8 9 10
Genetic drift
• change in allele frequency between generations due to the random sampling of alleles
• large population, allele and genotype frequencies predictable
• smaller populations, random chance (drift) becomes important
Genetic drift...1. Changes allele frequencies of populations
2. Reduces genetic variation of populations
3. Is random: same starting point => different outcomes
4. Depends on population size (small pops have strong drift)
N = 4 N = 40 N = 400
Bottlenecks & founder effects
Bottleneck = drift due to a drastic reduction in population sizeFounder effect = bottleneck associated with the founding of a
new population
The Equilibrium Population:Assumptions of Hardy-Weinberg
• No mutation (no new genetic variation)
• No genetic drift (infinitely large population)
• No migration (no individuals entering or leaving the pop)
• No selection (genotypes have equal fitness)
• Random mating (dealing with a single population)
Migration = movement of individuals between populations
Gene flow = transfer of alleles from one population to another
Connectivity
AA
aaAAAA AA
AAAA aaAAAAAA
aaaaAA
aa
AAaa
Freq A = p = 1Freq a = q = 0
Freq A = p = 0Freq a = q = 1
Gene flow can be a strong evolutionary force
Gene flow can be a strong evolutionary force
One migration event => deviation from H-W expectations
AAaaAAAA AA
AAAAaa
AAAAAA
aaaaAA aa
AAaa
Freq A = p = 0.82Freq a = q = 0.18Would predict 2pq = 0.30Actual frequ of Aa = 0
Freq A = p = 0.33Freq a = q = 0.67Would predict 2pq = 0.44Actual frequ of Aa = 0
AAAaAAAA Aa
AAAAAAAa
Aaaa
aa
Ongoing gene flow prevents population divergence & (eventually) homogenizes allele frequencies
AaaaAAaa
AAAa
Gene flow can be a strong evolutionary force
Freq A = p = 0.67Freq a = q = 0.33Would predict 2pq = 0.44Actual frequ of Aa = 0.33
Freq A = p = 0.5Freq a = q = 0.5Would predict 2pq = 0.5Actual frequ of Aa = 0.33
The Equilibrium Population:Assumptions of Hardy-Weinberg
• No mutation (no new genetic variation)
• No genetic drift (infinitely large population)
• No migration (no individuals entering or leaving the pop)
• No selection (genotypes have equal fitness)
• Random mating (dealing with a single population)
Natural selection
beneficial mutation
(should increase in frequency)
Natural selection happensMany pathogens evolve resistance to antibiotics
Many pests evolve resistance to pesticidesNatural selection happens
Adaptation/diversification in higher eukaryotes - slower, but still going on
before selection
after selection(1977 drought)
mediumground
finch
Natural selection happens
Patterns of phenotypic selectionDirectional selection
trait value
frequ
ency
Stabilizing selection
trait value
frequ
ency
Patterns of phenotypic selection
Diversifying selection
trait value
frequ
ency
Patterns of phenotypic selection
Frequency-dependent selection
Morphs of a single Heliconius speciesNon-poisonous mimics of poisonous butterflies
=> each has higher fitness when rare
Patterns of phenotypic selection
Patterns of phenotypic selectionHeterozygous Advantage (Overdominance)