medical photonics lecture optical engineering · - at the geometrical shadow edge: 0.25 - shadow...
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Medical Photonics Lecture
Optical Engineering
Lecture 3: Diffraction
2019-04-24
Herbert Gross
Speaker: Yi Zhong
Summer term 2019
2
Contents
No Subject Ref Date Detailed Content
1 Introduction Zhong 10.04.Materials, dispersion, ray picture, geometrical approach, paraxial approximation
2 Geometrical optics Zhong 17.04.Ray tracing, matrix approach, aberrations, imaging, Lagrange invariant
3 Diffraction Zhong 24.04.Basic phenomena, wave optics, interference, diffraction calculation, point spread function, transfer function
4 Components Kempe 08.05. Lenses, micro-optics, mirrors, prisms, gratings
5 Optical systems Zhong 15.05.Field, aperture, pupil, magnification, infinity cases, lens makers formula, etendue, vignetting
6 Aberrations Zhong 22.05. Introduction, primary aberrations, miscellaneous
7 Image quality Zhong 29.05. Spot, ray aberration curves, PSF and MTF, criteria
8 Instruments I Kempe 05.06.Human eye, loupe, eyepieces, photographic lenses, zoom lenses, telescopes
9 Instruments II Kempe 12.06.Microscopic systems, micro objectives, illumination, scanning microscopes, contrasts
10 Instruments III Kempe 19.06.Medical optical systems, endoscopes, ophthalmic devices, surgical microscopes
11 Photometry Zhong 26.06.Notations, fundamental laws, Lambert source, radiative transfer, photometry of optical systems, color theory
12 Illumination systems Gross 03.07.Light sources, basic systems, quality criteria, nonsequential raytrace
13 Metrology Gross 10.07. Measurement of basic parameters, quality measurements
▪ Diffraction at an edge in Fresnel
approximation
▪ Intensity distribution,
Fresnel integrals C(x) and S(x)
scaled argument
▪ Intensity:
- at the geometrical shadow edge: 0.25
- shadow region: smooth profile
- bright region: oscillations
−+
−=
22
)(2
1)(
2
1
2
1)( tStCtI
FNxz
xz
kt 2
2=
=
=
Fresnel Edge Diffraction
t-4 -2 0 2 4 6
0
0.5
1
1.5
I(t)
3
▪ Diffraction at a slit:
intensity distribution
▪ Angles with side maxima:
constructive interference
▪ Diffraction pattern
d
0. order
1st diffraction
order
2
sin
sinsin
)(
=
d
d
I
+=
2
1sin max m
d
Fraunhofer Farfield Diffraction
order: -3rd -2nd -1st 0th +1st +2nd +3rd
4
Fraunhofer Diffraction at a Rectangle
▪ Diffraction at a square / rectangle shaped aperture
▪ Smaller width: larger diffraction angle
square aperture rectangular aperture
5
Diffraction at the System Aperture
▪ Self luminous points: emission of spherical waves
▪ Optical system: only a limited solid angle is propagated, the truncaton of the spherical wave
results in a finite angle light cone
▪ In the image space: uncomplete constructive interference of partial waves, the image point
is spreaded
▪ The optical systems works as a low pass filter
object
point
spherical
wave
truncated
spherical
wave
image
plane
x = 1.22 / NA
point
spread
function
object plane
6
Huygens Principle
wave fronts
Huygens-Wavelets
propagation direction
stop
geometrical shadow
7
PSF by Huygens Principle
▪ Huygens wavelets correspond to vectorial field components
▪ The phase is represented by the direction
▪ The amplitude is represented by the length
▪ Zeros in the diffraction pattern: destructive interference
▪ Aberrations from spherical wave: reduced conctructive superposition
pupil
stop
wave
front
ideal
reference
sphere
point
spread
function
zero
intensity
side lobe
peak
central peak maximum
constructive interference
reduced constructive
interference due to phase
aberration
8
▪ Typical change of the intensity profile
▪ Normalized coordinates
▪ Diffraction integral
9
Fresnel Diffraction
z
geometrical
focus
f
a
z
stop
far zone
geometrical
phase
intensity
a
rr
f
avz
f
au =
=
=
;
2;
22
( )
−
−=
1
0
20
/
2
0
2 22
)(2
),(
devJef
EiavuE
ui
uafi
▪ Focussed beam with various wavelengths
▪ Focal region given by Rayleigh length Ru = /NA2
▪ Geometrical cone outside focal region
▪ Diffraction structure in focal range
10
Geometrical vs Diffraction Ranges
= 1 mm = 2.5 mm = 5 mm = 10 mmA
1/2
geometrical
range
diffraction
focal range
▪ The LOmmel approach is valid for large Fresnel numbers
and takes the small NF-effects not into account
▪ Typical intensity distribution in logarithmic representation
11
Fresnel Diffraction
u
I(u,0)
I(u,0)
v
Log I(u,0)
a
a
zau 2;2
32
Fraunhofer Point Spread Function
▪ Rayleigh-Sommerfeld diffraction integral,
Mathematical formulation of the Huygens-principle
▪ Fraunhofer approximation in the far field
for large Fresnel number
▪ Optical systems: numerical aperture NA in image space
Pupil amplitude/transmission/illumination T(xp,yp)
Wave aberration W(xp,yp)
complex pupil function A(xp,yp)
Transition from exit pupil to
image plane
▪ Point spread function (PSF): Fourier transform of the complex pupil
function
1
2
=z
rN
p
F
),(2),(),( pp yxWi
pppp eyxTyxA
=
( ) ( ) ( )
pp
yyxxR
i
yxiW
pp
AP
dydxeeyxTyxEpp
APpp
''2
,2,)','(
+
=
''cos'
)'()('
dydxrr
erE
irE d
rrki
I −
−=−
12
( )( )
0
2
12,0 I
v
vJvI
= ( )
( )0
2
4/
4/sin0, I
u
uuI
=
-25 -20 -15 -10 -5 0 5 10 15 20 250,0
0,2
0,4
0,6
0,8
1,0
vertical
lateral
inte
nsity
u / v
Circular homogeneous illuminated
Aperture: intensity distribution
▪ transversal: Airy
scale:
▪ axial: sinc
scale
▪ Resolution transversal better
than axial: x < z
Ref: M. Kempe
Scaled coordinates according to Wolf :
axial : u = 2 z n / NA2
transversal : v = 2 x / NA
Perfect Point Spread Function
NADAiry
=
22.1
2NA
nRE
=
13
Ideal Psf
r
z
I(r,z)
lateral
Airy
axial
sinc2
aperture
cone image
plane
optical
axis
focal point
spread spot
14
log I(r)
r0 5 10 15 20 25 30
10
10
10
10
10
10
10
-6
-5
-4
-3
-2
-1
0
Airy distribution:
▪ Gray scale picture
▪ Zeros non-equidistant
▪ Logarithmic scale
▪ Encircled energy
Perfect Lateral Point Spread Function: Airy
DAiry
r / rAiry
Ecirc
(r)
0
1
2 3 4 5
1.831 2.655 3.477
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
2. ring 2.79%
3. ring 1.48%
1. ring 7.26%
peak 83.8%
15
Abbe Resolution and Assumptions
Assumption Resolution enhancement
1 Circular pupil ring pupil, dipol, quadrupole
2 Perfect correction complex pupil masks
3 homogeneous illumination dipol, quadrupole
4 Illumination incoherent partial coherent illumination
5 no polarization special radiale polarization
6 Scalar approximation
7 stationary in time scanning, moving gratings
8 quasi monochromatic
9 circular symmetry oblique illumination
10 far field conditions near field conditions
11 linear emission/excitation non linear methods
▪ Abbe resolution with scaling to /NA:
Assumptions for this estimation and possible changes
▪ A resolution beyond the Abbe limit is only possible with violating of certain
assumptions
16
Defocussed Perfect Psf
▪ Perfect point spread function with defocus
▪ Representation with constant energy: extreme large dynamic changes
z = -2RE z = +2REz = -1RE z = +1RE
normalized
intensity
constant
energy
focus
Imax = 5.1% Imax = 42%Imax = 9.8%
17
▪ Normalized axial intensity
for uniform pupil amplitude
▪ Decrease of intensity onto 80%:
▪ Scaling measure: Rayleigh length
- wave optical definition
depth of focus: 1RE
- Gaussian beams: similar formula
22
'
'sin' NA
n
unRu
==
Depth of Focus: Diffraction Consideration
2
0
sin)(
=
u
uIuI
2' o
un
R
=
udiff Run
z
=2
1
sin493.0
2
12
focal
plane
beam
caustic
z
depth of focus
0.8
1
I(z)
z-Ru/2 0
r
intensity
at r = 0
+Ru/2
18
Psf with Aberrations
▪ Psf for some low oder Zernike coefficients
▪ The coefficients are changed between cj = 0...0.7
▪ The peak intensities are renormalized
spherical
defocus
coma
astigmatism
trefoil
spherical
5. order
astigmatism
5. order
coma
5. order
c = 0.0
c = 0.1c = 0.2
c = 0.3c = 0.4
c = 0.5c = 0.7
19
( )( )0,0
0,0)(
)(
ideal
PSF
real
PSFS
I
ID =
2
2),(2
),(
),(
=
dydxyxA
dydxeyxAD
yxWi
S
▪ Important citerion for diffraction limited systems:
Strehl ratio (Strehl definition)
Ratio of real peak intensity (with aberrations) referenced on ideal peak intensity
▪ DS takes values between 0...1
DS = 1 is perfect
▪ Critical in use: the complete
information is reduced to only one
number
▪ The criterion is useful for 'good'
systems with values Ds > 0.5
Strehl Ratio
r
1
peak reduced
Strehl ratio
distribution
broadened
ideal , without
aberrations
real with
aberrations
I ( x )
20
Point Spread Function with Apodization
w
I(w)
1
0.8
0.6
0.4
0.2
00 1 2 3-2 -1
Airy
Bessel
Gauss
FWHM
w
E(w)
1
0.8
0.6
0.4
0.2
03 41 2
Airy
Bessel
Gauss
E95%
▪ Apodisation of the pupil:
1. Homogeneous
2. Gaussian
3. Bessel
▪ Psf in focus:
different convergence to zero forlarger radii
▪ Encircled energy:
same behavior
▪ Complicated:Definition of compactness of thecentral peak:
1. FWHM: Airy more compact as GaussBessel more compact as Airy
2. Energy 95%: Gauss more compact as AiryBessel extremly worse
21
▪ Small aperture:
Diffraction limited
Spot size corresponds
to Airy diameter
Spot size depends on
wavelength
▪ Large aperture:
Diffraction neglectible
Aberration limited
Geometrical effects not
wavelength dependent
But: small influence of
dispersion
Log Dfoc
Log sinu
f=1000 , 500 , 200 , 100 , 50 , 20 , 10 mm
= 10 m m
m
= 1 m
550 nm
1
0
-1
-2-2-3 -1 0
Focussing by a Lens: Diffraction and Aberration
22
x
y
▪ PSF as a function
of the field height
▪ Interpolation is
critical
▪ Orientation of coma
in the field
▪ Small field area with
approximately shift-
invariant PSF:
isoplanatic patch
Variation of Performance with Field Position
x = 0 x = 20% x = 40% x = 60 % x = 80 % x = 100 %
23
▪ Growing spherical aberration shows an asymmetric behavior around the nominal image
plane for defocussing
24
Caustic with Spherical aberration
c9 = 0 c9 = 0.7c9 = 0.3 c9 = 1
Low Fresnel Number Focussing
25
▪ Small Fresnel number NF:
geometrical focal point (center point of spherical wave) not identical with best
focus (peak of intensity)
▪ Optimal intensity is located towards optical system (pupil)
▪ Focal caustic asymmetrical around the peak location
a
focal length f
z
physical
focal
point
geometrical
focal point
focal shift
f
wavefront
Low Fresnel Number Focussing
26
▪ System with small Fresnel number:
Axial intensity distribution I(z) is
asymmetric
▪ Explanation of this fact:
The photometric distance law
shows effects inside the depth of focus
▪ Example microscopic 100x0.9 system:
a = 1mm , z = 100 mm:
NF = 18
2
2
0
4
4sin
21)(
−=
u
u
N
uIzI
F
-0.8 -0.6 -0.4 -0.2 0 0.2 0.4 0.6 0.8 1 1.20
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
NF = 2
NF = 3.5
NF = 5
NF = 7.5
NF = 10
NF = 20
NF = 100
z / f - 1
I(z)
▪ Diffraction at an edge in Fresnel
approximation
▪ Intensity distribution,
Fresnel integrals C(x) and S(x)
scaled argument
▪ Intensity:
- at the geometrical shadow edge: 0.25
- shadow region: smooth profile
- bright region: oscillations
−+
−=
22
)(2
1)(
2
1
2
1)( tStCtI
FNxz
xz
kt 2
2=
=
=
Fresnel Edge Diffraction
t-4 -2 0 2 4 6
0
0.5
1
1.5
I(t)
27
▪ Line image: integral over point sptread function
LSF: line spread function
▪ Realization: narrow slit
convolution of slit width
▪ But with deconvolution, the PSF can be reconstructed
?= dyyxIxI PSFLSF ),()(
Integration
intens
ity
x
Line spread function
PSF
= dyyxIxI PSFLSF ),()(
Line Image
28
ESF, PSF and ESF-Gradient
▪ Typical behavior of intensity of an edge image for residual aberrations
▪ The width of the distribution roughly corresponds to the diameter of the PSF
▪ Derivative of the edge spread function:
edge position at peak
location
y'0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
-0.1 -0.05 0 0.05 0.1 0.15
derivation of
edge spread
function
edge spread
function
point spread
function
29
diffracted ray
direction
object
structure
g = 1 /
= / g
k
kT
Definitions of Fourier Optics
▪ Phase space with spatial coordinate x and
1. angle
2. spatial frequency in mm-1
3. transverse wavenumber kx
▪ Fourier spectrum
corresponds to a plane wave expansion
▪ Diffraction at a grating with period g:
deviation angle of first diffraction order varies linear with = 1/g
0k
kv x
xx ==
),(ˆ),( yxEFvvA yx =
( ) ( )A k k z E x y z e dx dyx y
i xk ykx y, , ( , , )=− +
vk 2=
vg
== 1
sin
30
▪ Arbitrary object expaneded into a
spatial
frequency spectrum by Fourier
transform
▪ Every frequency component is
considered separately
▪ To resolve a spatial detail, at least
two orders must be supported by the
system
NAg
mg
==
=
sin
sin
off-axis
illumination
NAg
=
2
Ref: M. Kempe
Grating Diffraction and Resolution
optical
system
object
diffracted orders
a)
resolved
b) not
resolved
+1.
+1.
+2.
+2.
0.
-2.
-1.
0.
-2.
-1.
incident
light
+1.
0.
+2.
+1.
0.
+2.
-2.
-2. -1.
-1.
+3.+3.
31
Number of Supported Orders
▪ A structure of the object is resolved, if the first diffraction order is propagated
through the optical imaging system
▪ The fidelity of the image increases with the number of propagated diffracted orders
0. / +1. / -1. order
0. / +1. / -1.
+2. / -2.
order
0. / +1. -1. / +2. /
-2. / +3. / -3.
order
32
▪ Location of diffraction orders in the
back focal plane
▪ Increasing grating angle with spatial
frequency of the grating
33
Abbe Theory of Microscopic Resolution
+1st
-1st
+1st
-1st
+1st
-1st
objectback focal
planeobjective
lens
0th order
Resolution of Fourier Components
Ref: D.Aronstein / J. Bentley
object
pointlow spatial
frequencies
high spatial
frequencies
high spatial
frequencies
numerical aperture
resolved
frequencies
object
object detail
decomposition
of Fourier
components
(sin waves)
image for
low NA
image for
high NA
object
sum
34
( )
pppp
pp
vyvxi
pp
yxOTF
dydxyxg
dydxeyxg
vvH
ypxp
−
−
−
−
+−
=2
22
),(
),(
),(
),(ˆ),( yxIFvvH PSFyxOTF =
pppp
pp
y
px
p
y
px
p
yxOTF
dydxyxP
dydxvf
yvf
xPvf
yvf
xP
vvH
−
−
−
−
−−++
=2
*
),(
)2
,2
()2
,2
(
),(
Optical Transfer Function: Definition
▪ Normalized optical transfer function
(OTF) in frequency space
▪ Fourier transform of the Psf-
intensity
▪ OTF: Autocorrelation of shifted pupil function, Duffieux-integral
▪ Absolute value of OTF: modulation transfer function (MTF)
▪ MTF is numerically identical to contrast of the image of a sine grating at the
corresponding spatial frequency
35
x p
y p
area of
integration
shifted pupil
areas
f x
y f
p
q
x
y
x
y
L
L
x
y
o
o
x'
y'
p
p
light
source
condenser
conjugate to object pupil
object
objective
pupil
direct
light
at object diffracted
light in 1st order
Interpretation of the Duffieux Iintegral
▪ Interpretation of the Duffieux integral:
overlap area of 0th and 1st diffraction order,
interference between the two orders
▪ The area of the overlap corresponds to the
information transfer of the structural details
▪ Frequency limit of resolution:
areas completely separated
36
Optical Transfer Function of a Perfect System
▪ Aberration free circular pupil:
Reference frequency
▪ Cut-off frequency:
▪ Analytical representation
▪ Separation of the complex OTF function into:
- absolute value: modulation transfer MTF
- phase value: phase transfer function PTF
'sin u
f
avo ==
'sin222 0
un
f
navvG ===
−
−
=
2
000 21
22arccos
2)(
v
v
v
v
v
vvHMTF
),(),(),( yxPTF vvHi
yxMTFyxOTF evvHvvH =
/ max
00
1
0.5 1
0.5
gMTF
37
I Imax V
0.010 0.990 0.980
0.020 0.980 0.961
0.050 0.950 0.905
0.100 0.900 0.818
0.111 0.889 0.800
0.150 0.850 0.739
0.200 0.800 0.667
0.300 0.700 0.538
Contrast / Visibility
▪ The MTF-value corresponds to the intensity contrast of an imaged sin grating
▪ Visibility
▪ The maximum value of the intensity
is not identical to the contrast value
since the minimal value is finite too
▪ Concrete values:
minmax
minmax
II
IIV
+
−=
I(x)
-2 -1.5 -1 -0.5 0 1 1.5 2
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
x
Imax
Imin
object
image
peak
decreased
slope
decreased
minima
increased
38
▪ Real MTF of system with residual aberrations:
1. contrast decreases with defocus
2. higher spatial frequencies have stronger decrease
Real MTF
z = 0
z = 0.1 Ru
gMTF
1
0.75
0.25
0.5
0
-0.250 0.2 0.4 0.6 0.8 1
z = 0.2 Ru
z = 0.3 Ru
z = 1.0 Ru
z = 0.5 Ru
-100 -50 0 50 1000
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
gMTF
(z,f)
z in
RU
max
= 0.05
max
= 0.1
max
= 0.2
max
= 0.3
max
= 0.4
max
= 0.5
max
= 0.6
max
= 0.7
max
= 0.8
Zernike
coefficients:
c5 = 0.02
c7 = 0.025
c8 = 0.03
c9 = 0.05
39
▪ Contrast vs contrast as a function of spatial frequency
▪ Typical: contrast reduced for
increasing frequency
▪ Compromise between
resolution and visibilty
is not trivial and depends
on application
Contrast and Resolution
V
c
1
010
HMTF
Contrast
sensitivity
HCSF
40
Contrast / Resolution of Real Images
resolution,
sharpness
contrast,
saturation
▪ Degradation due to
1. loss of contrast
2. loss of resolution
41
Modulation Transfer
Convolution of the object intensity distribution I(x) changes:
1. Peaks are reduced
2. Minima are raised
3. Steep slopes are declined
4. Contrast is decreased
I(x)
x
original image
high resolving image
low resolving image
Imax-Imin
42
Test: Siemens Star
Determination of resolution and contrast
with Siemens star test chart:
▪ Central segments b/w
▪ Growing spatial frequency towards the
center
▪ Gray ring zones: contrast zero
▪ Calibrating spatial feature size by radial
diameter
▪ Nested gray rings with finite contrast
in between:
contrast reversal, pseudo resolution
43
Resolution Test Chart: Siemens Star
original good system
astigmatism comaspherical
defocusa. b. c.
d. e. f.
44
Fourier Optics – Point Spread Function
▪ Optical system with magnification m
Pupil function P,
Pupil coordinates xp,yp
▪ PSF is Fourier transform
of the pupil function
(scaled coordinates)
( )( ) ( )
−+−−
= pp
myyymxxxz
ik
pppsf dydxeyxPNyxyxgpp ''
,)',',,(
( ) pppsf yxPFNyxg ,ˆ),( =
object
planeimage
plane
source
point
point
image
distribution
45
Fourier Theory of Incoherent Image Formation
objectintensity image
intensity
single
psf
object
planeimage
plane
▪ Transfer of an extended
object distribution I(x,y)
▪ In the case of shift invariance
(isoplanasy):
incoherent convolution
▪ Intensities are additive
dydxyxIyyxxgyxI psfinc
−
−
−−= ),()','()','(2
),(*),()','( yxIyxIyxI objpsfimage =
dydxyxIyyxxgyxI psfinc
−
−
= ),(),',,'()','(2
46
Fourier Theory of Incoherent Image Formation
object
intensity
I(x,y)
squared PSF,
intensity-
response
Ipsf
(xp,y
p)
image
intensity
I'(x',y')
convolution
result
object
intensity
spectrum
I(vx,v
y)
optical
transfer
function
HOTF
(vx,v
y)
image
intensity
spectrum
I'(vx',v
y')
produkt
result
Fourier
transform
Fourier
transform
Fourier
transform
47
Incoherent Image Formation
object
-0.05 0 0.05
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
-0.05 0 0.05
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
-0.05 0 0.05
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
-0.05 0 0.05
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
-0.05 0 0.05
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
-0.05 0 0.05
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
resolved not resolved▪ Example:
incoherent imaging of bar pattern near the
resolution limit
48
▪ Blurred imaging:
- limiting case
- information extractable
▪ Blurred imaging:
- information is lost
- what‘s the time ?
Resolution: Loss of Information
49
▪ Typical Psf of the human eye
▪ Change with pupil diameter size:
D = 2 mm : diffraction limited, smallest value
D < 2 mm : broadened due to diffraction
D > 2 mm : broadened due to aberrations (spherical)
50
Human Eye Performance
Ref. G. Yoon
Testchart Acuity of the Eye
Testchart with reduced resolution
51
Visual Acuity
▪ Recognition of simple geometrical
shapes :
1. Landolt ring with gap
2. Letter 'E'
▪ Blur of image on retina with distance
a)
5a
a
a
3a
3a
a
3a
b)
distance 6.096 m
block
letter
E 8.9
mm
image
height
25 mmeye
original blur : a/2 blur : a blur : 2a blur : 3a blur : 4a
3a
52