membrane-initiated estrogen signaling in the ovine endometrium membrane-initiated estrogen signaling...
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![Page 1: Membrane-Initiated Estrogen Signaling in the Ovine Endometrium Membrane-Initiated Estrogen Signaling in the Ovine Endometrium Brian Kitamura Dr. Fred Stormshak](https://reader035.vdocuments.net/reader035/viewer/2022062407/56649d5e5503460f94a3e751/html5/thumbnails/1.jpg)
Membrane-Initiated Membrane-Initiated Estrogen Signaling in Estrogen Signaling in
the Ovine the Ovine EndometriumEndometrium
Brian KitamuraBrian Kitamura
Dr. Fred StormshakDr. Fred Stormshak
Cecily BishopCecily Bishop
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RelevanceRelevance
Economic ImpactEconomic Impact Implications for reproduction in litter Implications for reproduction in litter
bearing speciesbearing species Conceptus implantation/attachmentConceptus implantation/attachment
Human HealthHuman Health Breast cancer/uterine cancerBreast cancer/uterine cancer Hypothesized to cause cells to multiplyHypothesized to cause cells to multiply Fast acting estrogen effects for Fast acting estrogen effects for
vasodilationvasodilation
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RationaleRationaleClassical Method of Steroid ActionClassical Method of Steroid Action
Protein
SR
HSP90
S 1
2
3
7
4
5
mRNA
Nucleus
6
SBP S
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RationaleRationalePrevious ObservationsPrevious Observations
In vitroIn vitro studies studies High affinity Estradiol (EHigh affinity Estradiol (E22)) binding sites in plasma binding sites in plasma
membrane of Chinese Hamster Ovary cells membrane of Chinese Hamster Ovary cells (Razandi (Razandi et alet al.).)
Rapid activation of MAPK pathway by ERapid activation of MAPK pathway by E2 2 (Song (Song et et alal.).)
In vivoIn vivo studies studies Winter 2004-2005Winter 2004-2005 High affinity EHigh affinity E22 binding sites in ovine binding sites in ovine
endometriumendometrium KKDD = 2.13 x 10 = 2.13 x 10-13 -13 MM
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Objectives of ResearchObjectives of Research
EE22 effect on the MAPK Pathway has effect on the MAPK Pathway has been studied in been studied in in vitroin vitro studies studies
Is there physiological significance in Is there physiological significance in the animal model?the animal model?
Examine the effect of EExamine the effect of E22 on the on the MAPK pathway MAPK pathway in vivoin vivo
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MethodsMethods Ovariectomized Ovariectomized
subjectssubjects Tissue samples from Tissue samples from
ovine endometriumovine endometrium Endometrium is highly Endometrium is highly
responsive to Eresponsive to E22
Hormone treated to Hormone treated to mimic estrous cyclemimic estrous cycle
General anesthetic General anesthetic Uterus exposed via Uterus exposed via
midventral midventral laparotomylaparotomy
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MethodsMethods Uterus opened to Uterus opened to
reveal endometriumreveal endometrium First tissue sample First tissue sample
takentaken 5 micrograms E5 micrograms E2 2
injected via uterine injected via uterine arteryartery
Tissue collected at 5, Tissue collected at 5, 10, 15 and 30 minutes 10, 15 and 30 minutes post injection of Epost injection of E22
Tissue frozen at -80 CTissue frozen at -80 C Returned to laboratoryReturned to laboratory
(interior tissue of the uterus known as the endometrium) Figure courtesy of P.L. Senger, Pathways to Pregnancy and Parturition, First Revised edition, 1999
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MethodsMethodsTime SchemeTime Scheme
P4 Given
Days 3-7 Days 8-9
E2 Given
Days 1-2 Day 11Day 10
E2 Given Rest Surgery
Hormone Schedule
Samples Taken
10min 30 min
First Sample Taken, E2 Injected
5 min0 min 15 min
Experiment Timeline
Final Sample Taken
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MethodsMethodsOur HypothesisOur Hypothesis
picture courtesy of picture courtesy of http://www.biocarta.com/pathfiles/h_erkPathway.asphttp://www.biocarta.com/pathfiles/h_erkPathway.asp
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MethodsMethods Testing the HypothesisTesting the Hypothesis
Tissue homogenized and separated Tissue homogenized and separated into nuclear and cytosolic portionsinto nuclear and cytosolic portions
Proteins separated via western blotProteins separated via western blot Probed for presence of Probed for presence of
phosphorylated ERK1/ERK2phosphorylated ERK1/ERK2
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MethodsMethodsTesting the HypothesisTesting the Hypothesis
picture courtesy of picture courtesy of http://www.biocarta.com/pathfiles/h_erkPathway.asphttp://www.biocarta.com/pathfiles/h_erkPathway.asp
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ResultsResultsPresence of Phosphorylated Presence of Phosphorylated
ERK1/2ERK1/2
Minutes: 0 5 10 Minutes: 0 5 10 15 3015 30Relative Intensity:
5 7.36 45.5 26.67 8.75
3 9.45 39.67 27.33 10
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ConclusionConclusion
Exogenous estrogen acts via a Exogenous estrogen acts via a membrane generated signal to membrane generated signal to stimulate the MAPK pathway in the stimulate the MAPK pathway in the ovine endometriumovine endometrium
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AcknowledgementsAcknowledgements
Howard Hughes Medical IntershipHoward Hughes Medical Intership Undergraduate Research, Undergraduate Research,
Innovation, Scholarship, and Innovation, Scholarship, and Creativity (URISC) Creativity (URISC)
Department of Animal Sciences, Department of Animal Sciences, College of AgricultureCollege of Agriculture
Stormshak LabStormshak Lab
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Thank You!Thank You!