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    CIHS Journal 2008: Vol 3 No1 1

    Acupuncture Meridians exist in Dermis (Connective Tissues)

    Comparative studies of Electrical Potential Gradient

    and

    Direction of Current Flow in Epidermis and Dermis

    Hiroshi Motoyama, Ph.D., Ph.D.

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    Meridians Exist in Dermis2

    Contents

    Abstract ........................................................................................................................... 3

    I. Objectives ............................................................................................................ 3II. Experimental Method......................................................................................... 3

    1) Differential Amplifier and its Zero Adjustment [Fig. 1, Graph -1, 1-2 ]......... 3

    2) Dermal Electrical Potential Measurement at 3 Acupuncture points of the Triple

    Heater Meridian.................................................................................................... 4

    3) Epidermal Electrical Potential Measurement.................................................... 4

    4) Dermal and Epidermal Electrical Potential Measurements at 3 Acupuncture

    points of the Heart Constrictor Meridian.............................................................. 4

    5) Test Subjects ......................................................................................................... 4

    III. Statistical Analysis and Consideration.............................................................. 5

    1) Data of Electrical Potential Measurements and their Statistical Analysis [Graph

    3]........................................................................................................................... 5

    2) Dermal Electrical Potential Gradient and Direction of Current Flow .............. 5

    3) Epidermal Potential Gradient and Direction of Current Flow .......................... 9

    4) Comparison of Average Electrical Potential of Dermal and Epidermal

    measurements ......................................................................................................11

    IV. Conclusion ......................................................................................................... 12

    V. Postscript ........................................................................................................... 13

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    CIHS Journal 2008: Vol 3 No1 3

    Acupuncture Meridians exist in Dermis (Connective Tissues)

    Comparative studies of Electrical Potential Gradient

    and

    Direction of Current Flow in Epidermis and Dermis

    Abstract

    Epidermal and Dermal electrical potential measurements were performed at 3

    acupuncture points of the Heart Constrictor and the Triple Heater meridians and 2

    acupuncture points of the Lung meridian. Data analysis of these measurements in

    terms of electrical potential gradient and direction of current flow revealed the results

    consistent with those obtained from earlier experimental studies as reported in

    [Measurement of ki-energy, diagnosis and treatment by Motoyama, Tokyo 1985 ].

    It has been confirmed that, in Yin meridians such as Heart Constrictor meridian and

    Lung meridian, ki-energy flows from bottom to top1 while, in Triple Heater meridian,

    direction of the flow is from top to bottom,.

    In contrast to this the electrical potential gradient and direction of current flow as

    obtained by Epidermal potential measurements have been found not in agreement

    with the above described directions of the ki-energy flow.

    I.

    Objectives

    The objective of this study is to confirm and demonstrate that the acupuncture meridians

    exist in the dermis and that there is ki-energy flow in there, and furthermore that the

    meridians do not exist on the epidermis..

    II.

    Experimental Method

    1) Differential Amplifier and its Zero Adjustment [Fig. 1, Graph -1, 1-2 ]

    A special differential amplifier devised by Motoyama and Kinoshita2, designed and

    built by Kinoshita, which was further improved by Digitex LAB.CO.LTD, was used.

    (Its noise level was lower than typical electrometer made in USA.) Before

    performing measurement each negative input terminal was grounded. Likewise

    the indifferent electrode pasted on the outer skin surface of subjects left arm was

    also grounded. Under this grounded condition zero-adjustment was performed on

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    Meridians Exist in Dermis4

    the computer screen. Next, both positive and negative input terminals were

    inter-connected and further zero-adjustment was performed on the computer screen.

    The subject to be tested is then instructed to enter the shielded room, sit on thereclining chair and maintain stable posture.

    2) Dermal Electrical Potential Measurement at 3 Acupuncture points of the

    Triple Heater Meridian

    A special #3 needle (0.20mm diameter) with insulation resin coating such that

    electrical conduction is allowed only at its bottom tip where metal of the needle is

    exposed to 0.3mm length. The length of the needle was 1.3 sun. The needle was

    inserted to 3mm depth at about 45 degrees to the skin surface on left Yochi (TE 4),

    left Gaikan(TE5) and left Shiko (TE6) [Fig.2]. Each needle was connected to

    (+) electrode and electrical potential was measured continuously for 600 sec. When

    needle is inserted into the dermis, ionic polarization takes place between the

    stainless steel of the needle and ions in the dermis. It takes 5~10 minutes for the

    signal to stabilize. Electrical potential signal was read after it was sufficiently

    stabilized.

    3) Epidermal Electrical Potential Measurement

    Next, non-polarizable surface electrode(9.5 mm x 4.5 mm) made of silver/silver

    chloride compound was pasted on the skin surface at left Yochi, left Gaikan and left

    Shiko of the Triple Heater meridian, and their electrical potential was measured

    continuously for 600 sec.

    4) Dermal and Epidermal Electrical Potential Measurements at 3

    Acupuncture points of the Heart Constrictor Meridian

    By following the same measurement procedure used for acupuncture points of the

    Triple Heater meridian described above, Dermal and Epidermal electrical

    potentials at Gekimon (PC4), Kanshi (PC5) and Naikan (PC6) were measured for

    600 sec on a different day. [Fig.3]

    5) Test Subjects

    Total 10 subjects ( 5 males and 5 females )

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    CIHS Journal 2008: Vol 3 No1 5

    III. Statistical Analysis and Consideration

    1) Data of Electrical Potential Measurements and their Statistical Analysis

    [Graph 3]As shown in Graph 3 (Dermal potential measurement performed on subject

    N.Y.; date 2004.4.4), Dermal electrical potential is measured at each point.

    Then, electrical potential differences among the points are calculated to

    determine the direction of the potential gradients. By the same procedure,

    potential difference and the direction of the potential gradient are determined for

    the Epidermal potential measurements as well.

    Next, data are organized as shown in Table 1-1, from which Table 1-2 is produced.

    Chi squared test is then applied to the m x n table to test the directions of

    currents in the dermis or on the epidermis.

    2) Dermal Electrical Potential Gradient and Direction of Current Flow

    (1) Dermal Electrical Potential Gradient and Current Direction in Triple

    Heater Meridian

    According to Table 1-2, statistical test of frequencies of normal direction

    [YochiGaikanShiko] and reverse direction in terms of electrical potential

    gradient and current direction shows P=1.4 x 103 < 0.002, i.e., high level of

    significance. Here, normal direction or reverse direction refer to the direction

    of ki-energy flow which has been known clinically since ancient times.

    Namely, in Yang meridians ki-energy flows from top to bottom and in Yin

    meridians it flows from bottom to top. This fact was experimentally

    confirmed by Motoyama. [Ref: Fig 5, page 70 Measurement of ki-energy

    flow , Diagnosis and Treatment ] Furthermore, under normal conditions,

    Motoyama found that, in 6 pairs of Yin-Yang meridians, BP(ki-energy) of

    Yin meridian > BP(ki-energy) of Yang meridian. [refer to Table A,

    page 46 of the same reference]

    Table 1-2, which is produced from Table 1-1, shows that 3 subjects out of 10

    showed normal direction of current flow by [YochiGaikanShiko] and

    that 10 out of 10 showed normal direction by [GaikanShiko]. In contrast

    to this, [GaikanYochi], which means reverse electrical potential or

    reversed direction of ki-energy flow, is seen in 7 subjects out of 10.

    According to Table 1-2, the test result from 2

    is P=1.4 x 103

    < 0.002, which

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    Meridians Exist in Dermis6

    indicates frequency of normal flow direction in Yochi Gaikan Shiko in

    Triple Heater meridian is significantly higher than frequency of reverse flow

    direction in Gaikan Yochi.

    In Graph 2 it is to be noted that, when the needle is inserted into the dermis,

    the electrical potential varies greatly due to the onset of polarization between

    the needle and ions in the dermis. This polarization stabilizes in 5~10

    minutes. Dermal electrical potential reading at each acupuncture point is

    taken after stabilization of this polarization.

    Above facts imply following 3 things;-

    Between Gaikan and Shiko, the flow is [Gaikan Shiko], i.e., normal

    direction from top to bottom in 10 subjects out of 10.

    Between Yochi and Shiko, 3 out of 10 showed the flow direction of

    [YochiShiko], i.e., normal direction.

    Between Gaikan and Yochi 7 subjects out of 10 showed reverse direction

    of flow. Namely, [Gaikan Yochi] or electrical potential of Gaikan is

    higher than that of Yochi.

    Looking at Table 1-3, it is evident that Gaikan has the highest average

    electrical potential.(1161 mV), while that of Yochi was 1104 mV and that of

    Shiko was 215 mV. The fact that Gaikan shows highest potential might be

    explained as follows;

    Together with Naikan of Heart Constrictor meridian, Gaikan of Triple Heater

    meridian has been clinically known since ancient times to be the point of

    inter-connection through which Triple Heater meridian and Heart Constrictor

    meridian exchange the information. Therefore, as point for the

    transmission of information, it might require higher electrical potential.

    Lastly, as regards the electrical potential gradients of Triple Heater meridian,

    3 subjects out of 10 showed the flow of [YochiShiko], which is a normal

    direction. Furthermore, 10 out of 10 showed normal direction with respect

    to [Gaikan Shiko] and 3 out of 10 show normal directions in [Yochi

    Gaikan]. Thus, 16 cases of normal flow direction. In contrast to this

    cases of [Gaikan Yochi], i.e., reverse direction were 7 out of 10.

    Therefore, it can be said that, in Triple Heater meridian, normal flow, i.e.,

    cases of from top to bottom are more frequent that those of reverse

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    CIHS Journal 2008: Vol 3 No1 7

    direction.

    The reason why there are cases of reversed flow is presumably due to the

    reversal of [BP(ki-energy flow) of Heart Constrictor meridian BP (ki-energy

    flow) of Large Intestine(=Yang meridian) ]. Thus, electrical potential

    gradient or direction of ki-energy flow of Lung meridian is always in the

    normal direction or from bottom to top. [see Table 3-1]

    In contrast to this, with respect to Heart Constrictor meridian and Triple

    Heater meridian, when normal and reverse directions of Yin-Yang pair

    meridian are examined by AMI data, 7 out of 10 showed [Heart

    Constrictor < Triple Heater ] or reversed relation. [see Table 2-1] In his

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    Meridians Exist in Dermis8

    earlier publication titled Data analysis, Diagnosis and Method of

    Treatment by AMI, Motoyama pointed out that the internal organs

    corresponding to those meridians of reversed relation have greaterpropensity for disease due to ki-energy depletion in corresponding Ying

    meridians. This implies that in that particular meridian the ki-energy is

    not flowing properly causing decrease of ki-energy flow. In such

    meridians that show Yin-Yan reversal it appears that reversal of

    ki-energy flow is likely to occur. The mechanism for this phenomenon is

    yet to be studied.

    Stress of Modern Life causes the reversal in Heart Constrictor and Triple

    Heater meridians.

    The reversal in Heart Constrictor and Triple Heater is common in people

    of modern society who do not have organic disorder. [ref. Data analysis,

    Diagnosis and Method of Treatment by AMI by Motoyama.(to be

    published)] In many cases the stress affects the functions of heart.

    On the other hand the Triple Heater meridian reflects the fatigued

    condition of the whole body. Modern life causes stress and overwork,

    which in turn induces excess or deficiency and imbalance of ki-energy

    thereby giving rise to the reversal of [Heart Constrictor < Triple Heater].

    (3)Dermal Potential Gradient and Current Direction in Lung Meridian

    According to Table 3-2, derived from Table 3-1, ki-energy flows from

    Kosai to Taien, i.e., from bottom to top in all of 8 subjects. P of 2 =

    8.0, P = 4.7 x 103< 0.005 (2 = 7.9). This indicates that this result,

    i.e., ki-energy flow from Kosai to Taien, is not accidental but due to

    some underlying cause that exists in the meridian. In Yin meridians such

    as the Lung meridian it was already mentioned in III 2) 2 that the

    ki-energy flows from bottom to top. [Graph 3]

    In Large Intestine meridian(Yang meridian), which is in Yin-Yang pair

    relationship with the Lung meridian(Yin meridian), as evident in Table

    3-1, of the 8 subjects tested by AMI 7 did not show [Yin

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    CIHS Journal 2008: Vol 3 No1 9

    In contrast to this, as described earlier in III 2), (1)(2), in Heart

    Constrictor and Triple Heater meridians, reversal of [Heart Constrictor(Yin meridian) < Triple Heater (Yang meridian)] is observed and the

    direction of electrical potential gradient and ki-energy flow were also

    found reversed.

    Above observations imply that:-

    There is Yin-Yang relationship among meridians

    If normal relationship of [Yin > Yang] is not maintained,

    reversal or disturbance may occur in the flow of ki-energy.

    Therefore, in order to correct the direction of ki-energy flow it is

    important to perform treatment by applying needle placement or

    pressure stimulus at the Yu-point and Bo-point of the Yin

    meridian that shows such reversals. [ref Data analysis, Diagnosis and

    Method of Treatment by AMI Hiroshi Motoyama (to be published)]

    3) Epidermal Potential Gradient and Direction of Current Flow

    (1)

    Epidermal Potential and Current Direction in Triple Heater Meridian

    [Table 4-1, 4-2, 4-3]

    According to Table 4-2, gradients of epidermal(surface) electric

    potential and epidermal(surface) electric current do not have

    statistically significant differences in frequencies between the normal

    direction [Yochi Gaikan Shiko: from top to bottom] and the

    reversed direction [YochiGaikan Shiko: from bottom to top]. In

    contrast to this dermal measurements clearly showed the direction of

    [Gaikan Shiko: from top to bottom] in a statistically significant

    manner. [see Table 1-2] This provides one evidence that the

    acupuncture meridian exists in the dermis.

    As for the magnitude of electrical potential, average dermal values for

    Yochi , Gaikan and Shiko were 1104mV, 1161mV and 215mV

    respectively, while those of epidermal values were smaller being

    49mV, 52mV and 47mV respectively. [Table 4-3]

    Graph 4 shows that the polarization at the interface of skin surface and

    electrode is less than that of the dermal potential. As regards the

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    Meridians Exist in Dermis10

    potential differences between pairs of points on the Triple Heater

    meridian, the differences for [Yochi- Gaikan] and [Gaikan -Shiko] were

    in 10mV~30mV range. [Graph 4] In terms of the dermal potential,however, the differences were very large at about 1200mV for [Gaikan

    -Shiko] pair of the Triple Heater meridian. [see Graph 2] Thus, much

    larger current flows in the dermis from top to bottom direction than in

    the epidermis(surface). The test result of 2 test from Table 4-2 shows

    no significant difference in normal and reverse direction by epidermal

    (surface) electrical potential measurements.

    (2)Epidermal Potential and Current Direction in Heart Constrictor

    Meridian [Table 5-1, 5-2, 5-3]

    Table 5-2 implies that there is statistically significant difference between

    the frequencies of normal and reverse cases by epidermal(surface)

    electrical potential measurements. Chi square test indicates

    significance of P=4.5 x 103 < 0.005. 10 subjects out of 11 showed

    normal direction [KanshiNaikan], while 8 subjects out of 11 showed

    reverse direction [KanshiGekimon]. Thus, the electrical potential

    gradient and current flow by epidermal(surface) measurements yielded

    results such that both bottom to top and top to bottom directions are

    inter-mixed in statistically significant manner, in much the same way as

    in the case of dermal measurements.

    This result implies that the epidermal(surface) electrical potential

    gradient and surface electrical current may reflect those of the dermis to

    a certain degree.

    As shown in Table 2-4, in contrast to the average electrical potential in

    the dermis being 1107mV, that of the epidermis(surface) is found to be

    quite small at 28mV. Furthermore, there are statistically significant

    differences between the average potential values of the dermis and the

    epidermis(surface) at the 3 acupuncture points [Table 2-5]. This

    result is might be explained by the difference of electrical resistance

    present in the dermis and the epidermis (surface) as well as the

    presence of electromotive force in the dermis. (For electromotive force

    in the dermis, refer to Electromotive force in living body --to be

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    CIHS Journal 2008: Vol 3 No1 11

    published.).

    (3)Epidermal Potential and Current Direction in Lung Meridian (Table 6-1,

    6-2, 6-3)

    Chi square test of Table 6-2 gives 2 = 0.50 < 20.5 = 3.8. This

    implies that there is no statistically significant difference between the

    frequencies of normal cases and reverse cases in terms of electrical

    potential gradient or direction of current flow between [KosaiTaien]

    points. Thus, although dermal potential gradients and electric current

    showed clear directionality of [KosaiTaien](see Table 3-2),

    epidermal potential gradients and electric current do not have definite

    directionality. (see Graph 5).

    This result provides evidence that the meridian exists in the dermis and

    ki-energy flows in there.

    4) Comparison of Average Electrical Potential of Dermal and Epidermal

    measurements

    (1) Comparison of Dermal electrical potential of Heart Constrictor and

    Triple Heater meridians

    Table 7-1 shows that the average electrical potential of the 3 points

    satisfies [Heart Constrictor(Yin meridian): 1107mV > Triple Heater

    meridian(Yang meridian): 683mV]. This result reconfirms the

    experimental finding that [BP (ki-energy flow) of Yin meridian > BP

    (ki-energy flow) of Yang meridian] as demonstrated by Table A in page

    46 of Measurement of Ki-energy Flow, Diagnosis and Treatment

    (2)Comparison of Epidermal electrical potential of Heart Constrictor and

    Triple Heater meridians

    As evident in Table 7-2, average potential of the 3 points shows [Heart

    Constrictor(Yin meridian): 28mV < Triple Heater (Yang meridian):

    49mV], which does not satisfy the relationship of [Ki-energy of Yin

    meridian > Ki-energy of Yang meridian]. In contrast to this, as

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    Meridians Exist in Dermis12

    described earlier, dermal potential gradients and current flow satisfy

    the relationship of [Heart Constrictor meridian > Triple Heater meridian],

    thereby reflecting the presence of the meridian and the ki-energy flow.Thus, epidermal potential gradients and current direction are dissimilar

    to those of acupuncture meridians. This result is in agreement with

    what was described in III, 2) and 3) above.

    (3)

    On the difference of Dermal electrical potential gradient between

    Naikan(Heart Constrictor meridian) and Gaikan(Triple Heater

    meridian)

    According to Table 8, electrical potential of Gaikan (Triple Heater

    meridian) is higher than that of Naikan(Heart Constrictor meridian) in all

    subjects tested. Chi square test yields 2= 7.0 > 2.01 = 6.6, indicating

    that the frequency of cases such that [Electrical Potential of Naikan Heart Constrictor: 683mV].

    Nevertheless, relative potential between Naikan and Gaikan proves to be

    [Heart Constrictor < Triple Heater]. Naikan and Gaikan are special in

    that they are the points inter-connecting the Heart Constrictor meridian

    and the Triple Heater meridian. (see page 70-73, Measurement of

    ki-energy flow/diagnosis and treatment Hiroshi Motoyama).

    Might it be that between the acupuncture points forming a pair

    inter-connecting two meridians of Yin-Yang correspondence,

    acupuncture point of the Yang meridian has higher electrical potential

    than that of Yin meridian? Many of the subjects tested this time

    showed reversal, i.e., [Heart Constrictor meridian < Triple Heater

    meridian]. Whether this fact has any relevance to the reversal observed

    at those inter-connecting points, i.e., Naikan and Gaikan, is yet to be

    clarified.

    IV.

    Conclusion

    Above considerations lead to following conclusions;-

    1)

    With respect to the direction of dermal electrical potential and the ki-energy

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    CIHS Journal 2008: Vol 3 No1 13

    flow, results obtained this time reinforce those of Motoyamas earlier

    experiments and provides further evidence for the existence of acupuncture

    meridians, existence of Yin and Yang distinctions as well as the direction ofthe ki-energy flow, showing coincidence with the direction of ki-energy flow,

    i.e., bottom to top in Yin meridian and top to bottom in Yang meridian, which

    has been clinically known since ancient days. (Ref. III, 2) (1), (2), (3) )

    2) Directions of epidermal potential gradients and electric currents do not

    show well-defined directionality such as bottom to top in Yin meridian and

    top to bottom in Yang meridian. Therefore, it is evident that epidermal

    electrical potential gradient and electric current do not have close linkage

    with acupuncture meridians. (III,2),(1), (2), (3))

    3) In Yin-Yang meridian pairs such as Heart Constrictor and Triple Heater

    meridians, if they show [Yin < Yang] reversal, ki-energy flow may assume

    both normal and reverse directions. (III, 1), (1),(2))

    4) In Yin-Yang meridian pairs which do not show such reversals, e.g., Lung

    meridian(Yin) and Large Intestine meridian(Yang), ki-energy flow is in

    normal direction, i.e., bottom to top, in most subjects.

    5) Acupuncture meridians exist in the dermis.

    6)

    Conclusion

    A)

    For Yang meridians direction of electrical potential gradient and current

    flow is from top to bottom irrespective of the BP values of the Yin-Yang

    pair being normal or reversed, i.e., whether [Yin BP > Yang BP] or

    [Yin BP < Yang BP].

    B)

    The Yin-Yang reversal, i.e., cases of [Yin BP< Yang BP], implies that the

    cause for the reversal lies in the Yin meridian of the Yin-Yang pair.

    V.

    Postscript

    In my earlier paper Acupuncture Meridians exist in Dermis no measurement of

    dermal electrical potential gradient and direction of current flow was performed for the

    Large Intestine meridian. Further study was performed this time not only with respect to

    the Large Intestine (Yang) meridian, which is in Yin-Yang pair relationship with the

    Lung (Yin) meridian, but also with respect to the Stomach (Yang) and the Spleen (Yin)

    meridians, another Yin-Yang pair. Dermal electrical potential gradient and direction of

    current flow were measured by the same experimental setup and procedures as

    described in the earlier paper. Chi-squares test was applied to collected data with the

    earlier experiment data and it has been confirmed once again that the direction of

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    Meridians Exist in Dermis14

    current flow in the dermis is from bottom to top for Yin meridians and from top to

    bottom for Yang meridians. The results obtained this time are presented here as

    supplement for the earlier study.

    In consideration of the observation that, where [Yin BP>Yang BP] relation is reversed,

    reversal of electrical potential gradient and direction of current is occasionally detected,

    BP measurement was first performed by AMI for each subject to determine groupings in

    terms of [Yin BP>Yang BP] (normal) or [Yin BP yang BP

    and yin BP < yang BP were counted by meridian name and by current flow direction.

    Result is summarized in Table 13.

    Set of numbers were extracted from Table 13 to produce (m x n) tables for Chi-squares

    test as follows;

    Chi-squares test applied to Table 14, which includes both Yin-Yang normal and

    Yin-Yang reversed cases, shows that there is statistically significant(4.7104 )

    difference in the frequency of occurrences between the normal direction and

    reversed direction of the current flow. This means that in Ying meridians Ki-energy

    flow is more frequently from bottom to top while in Yang meridians it is from top to

    bottom. Careful scrutiny of the Table 13 and Table 14 indicates that in all Yang

    meridians the Ki-energy(BP) flows from top to bottom (Yu-points-Internal Organs)

    without exception. (Concerning the mechanism of this phenomenon refer to Study of

    Nerves and Meridians by AMIand Measurement of ki-energy Flow, Diagnosis and

    Treatment by Hiroshi Motoyama)

    It is to be noted that, among the Yin meridians, Lung meridian showed normal

    (bottom-to-top) flow in all the 8 subjects. However, in Spleen (Yin) meridian, of the

    total 6 subjects 4 showed normal (bottom-to-top) flow but 2 showed reversed

    (top-to-bottom) flow.

    More cases of reversed flow were detected for Heart Constrictor (Yin) meridian; only 3

    cases were normal and 7 cases were reversed.

    Therefore, it may be concluded that in Yang meridians Ki-energy in general flows in

    normal (top-to-bottom) direction but that the Ki-energy flow direction may be

    reversed in Yin meridians.

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    CIHS Journal 2008: Vol 3 No1 15

    By dividing the Table 14 into cases of [Yin BP>Yang BP] and those of [Yin BP

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    Meridians Exist in Dermis16

    Intestine meridian. Furthermore, in pairs of meridians that are in Yin-Yang

    relationship,e.g.,Lung(Yin)-Large Intestine(Yang) and

    Spleen(Yin)-Stomach(Yang), it was shown that the direction of Ki-energyflow is from bottom to top for Yin meridians and from top to bottom for Yang

    meridians.

    It is important to note that in case of the Stomach, Large Intestine and

    Triple Heater meridians, which are Yang meridians, the directions of

    electrical potential gradient and current flow were normal, i.e., from top to

    bottom for both groupings of [Yin BP>Yang BP](normal) and [Yin BP Yang BP](normal) grouping. This result

    implies that the Yin-Yang reversal, i.e., [Yin BP < Yang BP], primarily

    reflects the conditions of the Yin meridian.

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    CIHS Journal 2008: Vol 3 No1 17

    Tables, Figures and Graphs

    Figure 1: Basic Circuit Diagram

    Graph 1-1: Electrometer when inputs were shorted (04/15/2004)

    -10-9

    -8

    -7

    -6

    -5

    -4

    -3

    -2

    -1

    0

    1

    2

    3

    4

    5

    6

    7

    89

    10

    0 500 1000 1500 2000 2500 3000 3500 4000 4500

    time[msec]

    Voltage[mV]

    Sampling Time = 1msec

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    Meridians Exist in Dermis18

    Graph 1-2: Noise when differential amplifier inputs are shorted: Gain= x1

    (2004/03/24)

    -10

    -9-8

    -7

    -6

    -5

    -4

    -3

    -2

    -1

    0

    1

    2

    34

    5

    6

    7

    8

    9

    10

    0 100 200 300 400 500 600

    time [sec]

    Voltage[mV]

    1CH

    Sampling Time = 100msec

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    CIHS Journal 2008: Vol 3 No1 19

    Figure 2: Triple Heater Meridian Figure 3: Heart Constrictor

    Meridian

    (Yochi, Gaikan, Siko) (Naikan, Kansi, Gekimon)

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    Meridians Exist in Dermis20

    Graph 3: Dermal potential- N.Y (male, 2004.4.4)

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    CIHS Journal 2008: Vol 3 No1 21

    Table 1-1 Triple Heater Meridian: Dermal Potential and Direction of Electric Current

    (unit of potential = mV; unit of BP = A)

    No. subjectMale

    /FemaleMeridianPoints

    leftYochi

    leftGaikan

    leftShiko

    Reversal by BP (HeartConstrictor

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    Meridians Exist in Dermis22

    Table 1-2: Triple Heater Meridian: Dermal Potential and Direction of Electric

    Current

    Dermal Potential and Direction of Current Flow

    Meridian. Point Yochi-Shiko*1

    Gaikan-Shiko*2

    Yochi-Gaikan*3

    Normal 3 10 3Reversed 0 0 7

    *1: "Normal" in the order of YochiGaikanShiko*2: "Normal" in the order of GaikanShiko*3: "Normal" in the order of YochiGaikan

    x Table

    2Test Result

    2

    13

    1.410-3

    0.600.75

    6.0

    Cramer's V

    2(0.95)

    Degree of Freedom

    2

    value

    (upper probability integral)Analysis Coefficient

    Table1-3: Average Dermal Potential of Triple Heater Meridian Points

    Meridian Point Average Dermal Potential(mV)

    Yochi 1104Gaikan 1161Shiko -215

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    CIHS Journal 2008: Vol 3 No1 23

    Graph 2: Dermal Potential: Triple Heater (Left Yochi, Left Gaikan, Left Sikou)

    N.Y. (male, 2004.1.25)

    -(-) input of each channel was grounded

    - indifferent electrode on outer left arm was also grounded

    - measurement was performed inside the shield room which was electrically grounded

    -Gain setting = x 1

    - Sampling time = 100ms

    Notes: Ch1(+)=Left Naikan, ch2(+)=Left Kanshi, ch3(+)=Left Gekimon, ch4(+)=Left Taien,

    ch5(+)=Left Kosai. Non-polarizable electrode attached to each point.

    Ch1(-)=ch2(-)=ch3(-)=ch4(-)=ch5(-)=connected to GND. Indifferent electrode pasted on outer

    left arm also connected to GND

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    Meridians Exist in Dermis24

    Table 2-1: Heart Constrictor Meridian: Dermal Potential and Direction of Electric

    Current

    (unit of potential = mV; unit of BP = A)

    No. subjectMale

    /FemaleMeridianPoints

    Gekimon Kanshi NaikanReversal by BP (Heart

    Constrictor

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    CIHS Journal 2008: Vol 3 No1 25

    Table 2-2: Heart Constrictor Meridian: Dermal Potential and Direction of Electric

    Current

    Meridian. Point ekimonKanshi*KanshiNaikan

    *2GekimonNaikan

    *3

    Normal 2 9 1Reversed 9 2 1

    *1"Normal" in the order of GekimonKanshi*2"Normal" in the order of KanshiNaikan*3"Normal" in the order of GekimonKanshiNaikan

    x Table

    2Test Result

    Analysis Coefficient

    Degree of Freedom 2

    8.9

    1.210-2

    Cramer's V

    2(0.95)

    0.61

    6.0

    0.52

    2

    value

    (upper probability integral)

    Table2-3: Average Dermal Potential of Heart Constrictor Meridian Points

    Meridian Point Average Dermal Potential(mV)Gekimon 1095Kanshi 1168

    Naikan 1059

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    Meridians Exist in Dermis26

    Fig 4: Left Lung Meridian Fig:5 Large Intestine Meridian

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    CIHS Journal 2008: Vol 3 No1 27

    Table 3-1: Lung Meridian: Dermal Potential and Direction of Electric Current

    (unit of potential = mV; unit of BP = A)

    No. subjectMale

    /FemaleMeridianPoints

    Kosai TaienReversal by BP (Lung < Large

    Intestine)*1 Su-Su F Potential 888 760 Normal

    Direction Lung(1656)L.I.(1396)2 Iw-Yu F Potential 799 675 Normal

    Direction Lung(1935)L.I.(1414)3 In-Ti F Potential 674 671 Normal

    Direction Lung(1124)L.I.(730)

    4 Tan-Hi M Potential 1040 590 NormalDirection Lung(1844)L.I.(1630)

    5 Tad-Hi M Potential 1171 618 NormalDirection Lung(1998)L.I.(1804)

    6 Ya-Na M Potential 674 419 NormalDirection Lung(1690)L.I.(1360)

    7 Ao-Ak M Potential 2500 2422 ReversedDirection Lung(1682)L.I.(1780)

    8 Wa-Ak M Potential 817 523 NormalDirection Lung(1708)L.I.(1336)

    1070 835Average of PotentialNote: "Normal" or "Reversed" are determined by comparing

    the BP values obtained by AMI measurements. (xxx)

    Table 3-2 : Lung Meridian: Dermal Potential and Direction of Electric Current

    Meridian Points KosaiTaien*

    2(0.95) 3.8

    Normal 8 Degrees of Freedom 1Reversed 0 No. of Items 2

    2

    8.0Normal 8Reversed 0

    *"Normal" in the order ofKosaiTaien

    Table3-3: Averages of Dermal Potential and Epidermal Potenrtial of Lung Meridian

    Points

    Meridian Points Average Dermal Potential(mV) Average Epidermal Potential(mV)Kosai 1070 19Taien 835 12

    Average 953 15

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    Meridians Exist in Dermis28

    Table 4-1: Triple Heater Meridian: Epidermal Potential and Direction of Electric

    Current

    (unit of potential = mV)

    No. subjectMale

    /FemaleMeridianPoints

    leftYochi

    leftGaikan

    leftShiko

    1 It-Ka F Potential 61 64 60Direction

    2 Tu-Em F Potential 30 27 24Direction

    3 In-Hi F Potential 35 35 27

    Direction4 Iw-Yu F Potential 104 108 101

    Direction5 In-Ti F Potential 10 26 14

    Direction6 Tan-Hi M Potential 121 113 119

    Direction7 Sa-Ke M Potential 53 52 51

    Direction8 Tad-Hi M Potential 21 26 28

    Direction9 Iw-Sh M Potential 14 24 -5

    Direction10 Ya-Na M Potential 39 47 48

    Direction49 52 47Average of Potential

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    CIHS Journal 2008: Vol 3 No1 29

    Table 4-2: Triple Heater Meridian: Epidermal Potential and Direction of Electric

    Current

    Meridian Points Yochi-Shiko*1

    Gaikan-Shiko*2

    Yochi-Gaikan*3

    Normal 3 7 4

    Reversed 2 3 6

    *1: "Normal" in the order of YochiGaikanShik

    *2: "Normal" in the order of GaikanShiko

    *3: "Normal" in the order of YochiGaikan

    x Table

    2Test Result

    2

    1.9

    0.39

    0.26

    0.27

    6.0

    Analysis Coefficient

    Cramer's V

    Degree of Freedom

    2

    value

    (upper probability integral)

    2(0.95)

    Note: mxn 2test on Table 4-2 shows that there is no

    statistically significant difference between the normal andrecersed directions.

    Table4-3: Average Epidermal Potential of Triple Heater Meridian Points

    Meridian Point Average Epidermal Potential(mV)

    Yochi 49

    Gaikan 52Shiko 47

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    Meridians Exist in Dermis30

    Graph 4: Epidermal Potential Left Triple Heater Meridian (Yochi, Gaikan, Shiko), I.S.

    Male 1.24.2004

    File Name: Electrodes on Left Yochi, Left Gaikan & Left Shiko 040124-02.OPG 2004/1/24 15:1:54 ID: Name IW-Sh Male

    Left Gaikan ch2(+)

    Left Yochi ch1(+)

    Left Shiko ch3(+)

    movedhead

    toleft

    scra

    tched

    ear

    by

    righ

    t

    10mV

    30mV

    (-) input of eachchannel wasgrounded

    indifferenteelctrode on outerleft arm was alsogrounded

    measuremet wasperformed inside theshielded room whichwas elec tricallygrounded

    Gain Setting = x 1

    Sampling time=100

    Epidermal Potentialat each point

    Notech1(+)=left yochich2(+)=left Gaikanch3(+)=left Shiko Non-polarized electrode attached to each point ch1(-)= ch2(-)=ch3(-) =connec ted to groundIndifferent electrode pasted on outer left arm was connected to ground

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    CIHS Journal 2008: Vol 3 No1 31

    Table 5-1: Heart Constrictor Meridian: Dermal Potential and Direction of Electric

    Current

    (unit of potential = mV)

    No. subjectMale

    /FemaMeridianPoints

    Gekimon Kanshi Naikan

    1 It-Ka F Potential 105 94 82Direction

    2 Su-Su F Potential 18 26 18Direction

    3 Iw-Yu F Potential 45 47 43Direction

    4 In-Ti F Potential 26 20 22Direction

    5 Tak-Ta F Potential 40 43 39Direction

    6 Tan-Hi M Potential 31 37 25Direction

    7 Tad-Hi M Potential -5 8 7Direction

    8 Iw-Sh M Potential 8 8 0Direction

    9 Ya-Na MPotential

    35 37 28Direction10 Ao-Ak M Potential -1 6 -11

    Direction11 Wa-Ak M Potential 15 17 16

    Direction29 31 25verage of Potentia

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    Meridians Exist in Dermis32

    Table 5-2: Heart Constrictor Meridian: Epiermal Potential and Direction of Electric

    Current

    Epidermal Potential and Direction of Current FlowMeridian. Points GekimonKanshi KanshiNaikan GekimonNaikan

    Normal 3 10 2Reversed 8 1 0

    *1"Normal" in the order of [GekimonKanshi*2 "Normal" in the order of [KanshiNaikan*3 "Normal" in the order of GekimonKanshiNaik

    x Table

    2Test Result

    2

    11

    4.510-3

    0.560.67

    6.0

    Cramer's V

    2(0.95)

    Degree of Freedom

    2

    value

    (upper probability integral)Analysis Coefficient

    Table 5-3: Average Epiermal Potential of Heart Constrictor Meridian Points

    Meridian Point Average Epidermal Potential(mV)Gekimon 29

    Kanshi 31Naikan 25

    Table 2-4: Heart Constrictor Meridian: Averages of Dermal Potential and Epidermal

    Potential

    Meridian. Points Average Dermal Potential(mV) Average Epidermal Potential(mV)

    Gekimon 1095 29

    Kanshi 1168 31

    Naikan 1059 25

    Average Potential 1107 28

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    CIHS Journal 2008: Vol 3 No1 33

    Table 2-5:t-test of the differences between Dermal potential and Epidermal potential

    measured at 3 meridian points of the Heart Constrictor meridian

    Left Gekimon

    No. subject Dermal Epidermal Dermal Epidermal1 It-Ka 869 105 Average 1095 29

    2 Su-Su 1152 18 Variance 2.4105

    9.0102

    3 Iw-Yu 1097 45 Number of samples 11 114 In-Ti 698 26 Pearson Correlation -0.385 Tak-Ta 928 40 Observed - Expected 0

    6 Tan-Hi 1036 31 Degrees of Freedom 107 Tad-Hi 1021 -5 t 7.0

    8 Iw-Sh 1142 8 P(T

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    Meridians Exist in Dermis34

    Table 6-1: Lung Meridian: Epidermal Potential and Direction of Electric Current

    (unit of potential = mV)

    No. subjectMale

    /FemaleMeridianPoints

    Kosai Taien

    1 Su-Su F Potential 19 3Direction

    2 Iw-Yu F Potential 44 39Direction

    3 In-Ti F Potential 21 23Direction

    4 Tan-Hi M Potential 35 10Direction5 Tad-Hi M Potential 8 11

    Direction6 Ya-Na M Potential 18 19

    Direction7 Ao-Ak M Potential -4 -17

    Direction8 Wa-Ak M Potential 12 6

    Direction19 12Average of Potential

    Table 6-2: Lung Meridian: Epidermal Potential and Direction of Electric Current

    Meridian Points KosaiTaien*

    2(0.95) 3.8

    Normal 5 Degrees of Freedom 1Reversed 3 Number of Samples 2

    2

    0.50Normal 5Reversed 3

    *"Normal" in the order ofKosaiTaien

    Table6-3 :Average Epidermal Potential of Lung Meridian Points

    Meridian PointKosaiTaien

    Average Epidermal Potential(mV)1912

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    CIHS Journal 2008: Vol 3 No1 35

    Graph 5: Dermal(skin surface) PotentialLeft Heart Constrictor meridian Naikan,

    Kanshi, Gekimon, Left Lung meridianTaien, Kosai I.T. Female 2004.04.04

    Graph 6 Dermal(skin surface) Potential Left Heart Constrictor meridian Naikan, Kanshi, Gekimon, Left Lung meridianTaien, Kosai I.T. Female 2004.04.04

    I.T.

    (-) input of eachchannel wasgrounded

    indifferentelectrode on outerleft arm was alsogrounded

    measurement waperformed insidethe shielded roomwhich waselectricallygrounded

    Gain setting = x 1

    Sampling Time= 100

    Left Kanshi ch2(+)Left Naikan ch1(+)

    Left Gekimon ch3(+)

    Left Kosai ch5(+) Left Taien ch4(+)

    bodymoveme

    nt

    movedhead

    shiftedbodyposition

    scratchedhead

    byright

    scratchedhead

    byright

    touchednosebyr

    ight

    Epidermal skin surface) Potential at each point

    Note ch1(+)=Left Naikanch2(+)=Left Kanshich3(+)=Left Gekimonch4(+)=Left Taiench5(+)=Left Kosai Non-polarizable electrode attached to each point. ch1(-)=ch2(-)=ch3(-)=ch4(-)=ch5(-)= connected to GND. Indifferent electrode pasted on outer left arm also connected to GND.

    bodymoveme

    nt

    bodymovem

    ent

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    Meridians Exist in Dermis36

    Table 7-1: Comparison of Dermal Potentials between Heart Constrictor and Triple

    Heater meridians

    H.C. Average of 3 H.C. pointsdermal 1107Gekimon Average of 3 T.H. pointsdermal 683KanshiNaikan Average of H.C.dermal Average of T.H.de

    Average of 3 pointsdermal 1107

    T.H.

    YochiGaikanShiko

    Average of 3 pointsdermal 683

    Average Dermal Potential(mV)109511681059

    Average Dermal Potential(mV)

    11041161-215

    Table 7-2: Comparison of Epidermal Potentials between Heart Constrictor and Triple Heater

    meridians

    H.C. Average of 3 H.C. pointsepidermal 28Gekimon Average of 3 T.H. pointsepidermal49Kanshi

    Naikan Average of H.C.epidermal < Average of T.H.epidermalAverage of 3 pointsepidermal 28

    T.H.YochiGaikanShiko

    Average of 3 pointsepidermal 49

    29Average Epidermal Potential(mV)

    495247

    31

    25

    Average Epidermal Potential(mV)

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    CIHS Journal 2008: Vol 3 No1 37

    Table 8: 2 value on frequencies of cases of [Naikan > Gaikan] and those of [Naikan