Metagenomics

Rob Edwards

MCS

The Soudan Mine, MinnesotaRed Stuff OxidizedBlack Stuff Reduced

Red and Black Samples Are Different

Cloned and 454 sequenced16S are indistinguishable

Black stuff

Red

ClonedRed

There are different amounts ofsubstrates in each environment

BlackStuff

RedStuff

There are different amounts of metabolism in each environment

Nitrification differentiates the samples

Edwards (2006)

The SEED metagenomics server

Metabolic potential in environments

Phylogenomics

Comparing Metagenomes to Genomes(or other metagenomes!)

Lots of sequencesall pyrosequencing

Metabolic potential in environments

Sulfur

CDA 60.2%

CD

A 2

1.7

% Respiration

Capsule Motility

Membranetransport

Stress

Signaling

Phosphorus

RNA

MineSaltern

MarineMicrobialites

CoralFish

AnimalsFreshwater

From sequences to environments

Dinsdale et al. unpublished