method 8290 dioxin-furan template

7/31/2019 Method 8290 Dioxin-Furan Template

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Tab 1 - Mass Calibration (Mass Spectrometer [MS] Instrument Resolution)

Peak Resolution for the Perfluorokerosene (PFK) Reference Ion resolution must be > 10,000 resolving power

(e.g. 10% Valley)

Resolving Power = A/(A-B)

where: A = mass/charge (e.g. [m/z] ration signal {380.97604})

B = peak width of ion at 5% of maximum signal (e.g. 380.93795)

SDG Experiment: Function: ppm Volts File: Date Ref. Time A B

Resolving

Power Pass/FailG578-34 EXP_DB5MS 3 200 0.0470 Res_Check 11-Jul-2009 PFK 9:24 380.97604 380.93795 10,001.99632 Pass

G578-35 EXP_DB5MS 1 200 0.0508 A09JUL09A_L 9-Jul-2009 PFK 17:39 380.97604 380.93795 10,001.99632 Pass



G578-35 EXP_DB5MS 1 200 0.0466 Res_Check 10-Jul-2009 PFK 5:45 380.97604 380.93795 10,001.99632 Pass










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Tab 2 - Spot Check Calculations Where:Qis = internal std. added (pg)

Analyte conc. [ug/L]= [Qis x (An1+An

2)]/[(Ais

1+Ais

2) x RRFn x W] An

1and An

2= area of primary and secondary analyte ions

Ais1

and Ais2

= area of primary and secondary standard ions (13

C)

RRFn = Relative Response Factor of analyteW = Dry weight of sample extracted (grams)

SDG

Calibrated

using Lab Std# Analyte Qis An

1An

2

.

before

drying(g)

%solids W Ais

1Ais

2RRFn

SGSLab

G578-29-1B 20 1,2,3,7,8,9-HxCDD (#5) 2000 4.59E+05 3.67E+05 11.48 81.2 9.32 4.12E+07 3.30E+07 0.9649 2.48

G578-29-1B 21 1,2,3,4,6,7,8-HpCDD (#6) 2000 9.65E+06 9.25E+06 11.48 81.2 9.32 3.06E+07 2.90E+07 1.0612 64.1

G578-29-1B 22 OCDD (#7) 4000 3.31E+08 3.70E+08 11.48 81.2 9.32 4.67E+07 5.23E+07 1.0643 2860

G578-29-1B 23 2,3,7,8-TCDF (#8) 2000 2.37E+05 2.64E+05 11.48 81.2 9.32 5.82E+07 7.36E+07 1.0368 EMPC

G578-33-1B 20 1,2,3,7,8,9-HxCDD (#5) 2000 3.49E+05 2.84E+05 10.54 85.1 8.97 4.90E+07 3.88E+07 0.9649 1.67

G578-33-1B 21 1,2,3,4,6,7,8-HpCDD (#6) 2000 8.07E+06 7.86E+06 10.54 85.1 8.97 3.25E+07 3.08E+07 1.0612 52.9

G578-33-1B 22 OCDD (#7) 4000 3.84E+08 4.30E+08 10.54 85.1 8.97 5.19E+07 5.79E+07 1.0643 3110

G578-33-1B 23 2,3,7,8-TCDF (#8) 2000 1.76E+05 2.52E+05 10.54 85.1 8.97 7.61E+07 9.56E+07 1.0368 0.538

G578-34-1B 20 1,2,3,7,8,9-HxCDD (#5) 2000 2.77E+05 2.40E+05 11.66 83.8 9.77 5.67E+07 4.50E+07 0.9649 1.08

G578-34-1B 21 1,2,3,4,6,7,8-HpCDD (#6) 2000 8.10E+06 7.84E+06 11.66 83.8 9.77 3.75E+07 3.53E+07 1.0612 42.2

G578-34-1B 22 OCDD (#7) 4000 5.24E+08 5.87E+08 11.66 83.8 9.77 6.02E+07 6.71E+07 1.0643 3360

G578-34-1B 23 2,3,7,8-TCDF (#8) 2000 1.09E+05 1.39E+05 11.66 83.8 9.77 5.06E+07 6.40E+07 1.0368 0.428

G578-35-1B 20 1,2,3,7,8,9-HxCDD (#5) 2000 3.75E+05 3.04E+05 11.72 88.4 10.36 5.50E+07 4.37E+07 0.9649 1.38

G578-35-1B 21 1,2,3,4,6,7,8-HpCDD (#6) 2000 8.92E+06 8.46E+06 11.72 88.4 10.36 3.94E+07 3.72E+07 1.0612 41.2

G578-35-1B 22 OCDD (#7) 4000 5.72E+08 6.40E+08 11.72 88.4 10.36 6.42E+07 7.13E+07 1.0643 3240

G578-35-1B 23 2,3,7,8-TCDF (#8) 2000 2.24E+05 2.75E+05 11.72 88.4 10.36 8.41E+07 1.06E+08 1.0368 0.488

G578-36-1B 20 1,2,3,7,8,9-HxCDD (#5) 2000 1.00E+05 7.81E+04 10.14 67.6 6.85 4.95E+07 3.93E+07 0.9649 0.607

G578-36-1B 21 1,2,3,4,6,7,8-HpCDD (#6) 2000 1.49E+06 1.46E+06 10.14 67.6 6.85 3.44E+07 3.29E+07 1.0612 12

G578-36-1B 22 OCDD (#7) 4000 6.28E+07 7.05E+07 10.14 67.6 6.85 4.41E+07 4.91E+07 1.0643 783

G578-36-1B 23 2,3,7,8-TCDF (#8) 2000 1.75E+05 2.31E+05 10.14 67.6 6.85 7.57E+07 9.57E+07 1.0368 0.668


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Tab 2 - Spot Check Calculations Where:Qis = internal std. added (pg)

Analyte conc. [ug/L]= [Qis x (An1+An

2)]/[(Ais

1+Ais

2) x RRFn x W] An

1and An

2= area of primary and secondary analyte ions

Ais1

and Ais2

= area of primary and secondary standard ions (13

C)

RRFn = Relative Response Factor of analyteW = Dry weight of sample extracted (grams)

SDG

Calibrated

using Lab Std# Analyte Qis An

1An

2

.

before

drying(g)

%solids W Ais

1Ais

2RRFn

SGSLab

Note: per Method 8290, if a 2,3,7,8-TCDF isomer is detected on the DB-5

column, it must be confirmed using a DB-225 column; the result must be

reported from the DB-225 column (this relates to DB-5 isomer co-elution

issues)

G578-29-1B & G578-2C - the 2,3,7,8-TCDF isomer result was

flagged as EMPC with no second column confirmation

G578-30-1B and -2B / G578-33-1B and -2B / G578-34-1B and -2B /

G578-35-2B / G578-36-1B, -2B, -3B, -4B, -5B the 2,3,7,8-TCDF

isomer had positive hit results but there was no confirmation on the

DB-255 column reported.

Note: per SGS lab "DB-225 confirmation only used for

2,3,7,8-TCDF if the concentration or EMPC value is 1

pg/g for a 10 gram dry wt. sample"


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na y e

conc.

[ug/L]Shaw

2.475

64.114

2855

0.787

1.666

52.878

3106

0.536

1.078

42.233

3357

0.427

1.376

41.274

3245

0.489

0.606

12.052

784

0.667


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Tab 3 - ION PAIR CHECK - Selected Ion Current Profile (SICP)

Descriptor:

Analyte:

Ion ID:

SDG File Acq. Date Time Sample ID Accurate Mass/Charge [m/z] Ratio:

G578-29-1B A07OCT08A 7-Oct-08 17.23 3 Time (min/sec):

G578-29-1B A07OCT08A 7-Oct-08 17.23 3G578-29-1B A07OCT08A 7-Oct-08 17.23 3

G578-30-1B A06JUL09C 6-Jul-09 16.41.32 2

G578-30-1B A06JUL09C 6-Jul-09 16.41.32 2

G578-30-1B A06JUL09C 6-Jul-09 16.41.32 2

G578-30-1B A06JUL09C 6-Jul-09 16.41.32 2

G578-33-1B A09JUL09A 9-Oct-08 17.59.38 10

G578-33-1B A09JUL09A 9-Oct-08 17.59.38 10

G578-33-1B A09JUL09A 9-Oct-08 17.59.38 10

G578-33-1B A09JUL09A 9-Oct-08 17.59.38 10

G578-33-1B A09JUL09A 9-Oct-08 17.59.38 10G578-33-1B A09JUL09A 9-Oct-08 17.59.38 10

G578-34-1B A09JUL09A_5 11-Jul-09 10.15.38 2

G578-34-1B A09JUL09A_5 11-Jul-09 10.15.38 2

G578-34-1B A09JUL09A_5 11-Jul-09 10.15.38 2

G578-34-1B A09JUL09A_5 11-Jul-09 10.15.38 2

G578-34-1B A09JUL09A_5 11-Jul-09 10.15.38 2

G578-34-1B A09JUL09A_5 11-Jul-09 10.15.38 2

G578-34-1B A09JUL09A_5 11-Jul-09 10.15.38 2

G578-34-1B A09JUL09A_5 11-Jul-09 10.15.38 2

G578-35-1B A09JUL09A_5 11-Jul-09 11.52.17 4

G578-35-1B A09JUL09A_5 11-Jul-09 11.52.17 4

G578-35-1B A09JUL09A_5 11-Jul-09 11.52.17 4

G578-35-1B A09JUL09A_5 11-Jul-09 11.52.17 4

G578-35-1B A09JUL09A_5 11-Jul-09 11.52.17 4

G578-35-1B A09JUL09A_5 11-Jul-09 11.52.17 4

G578-35-1B A09JUL09A_5 11-Jul-09 11.52.17 4

G578-35-1B A09JUL09A_5 11-Jul-09 11.52.17 4

G578-36-1B A28JUL09A 28-Jul-09 17.58.11

G578-36-1B A28JUL09A 28-Jul-09 17.58.11

G578-36-1B A28JUL09A 28-Jul-09 17.58.11

G578-36-1B A28JUL09A 28-Jul-09 17.58.11G578-36-1B A28JUL09A 28-Jul-09 17.58.11

G578-36-1B A28JUL09A 28-Jul-09 17.58.11


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2 2 3 3 3 3 3 3 4 4 4

HpCDPE PFK HxCDD HxCDD HxCDD (S) HxCDD (5) OCDPE PFK HpCDD HpCDD HpCDD (S)

M+2 LOCK M+2 M+4 M+2 M+4 M+4 LOCK M+2 M+4 M+2

409.7974 354.9792 389.8156 391.8127 401.8559 403.8529 445.7555 430.9728 423.7767 425.7737 435.8169

??? ??? 37.03 37.03 40.34 40.35 40.33

37.10 37.10 37.09 37.09

37.25 37.25 37.24 37.24

35.42 35.42 35.41 35.41 38.48 38.48 38.47

??? ??? 35.46 35.46

36.00 36.00 35.59 35.59

34.34 34.34 37.57 37.57

34.58 34.59 38.47 38.47 38.46

35.11 35.10

35.46 35.46 35.45 35.45

36.00 36.00 35.59 35.59

34.34 34.34 38.03 38.03

34.45 34.45 38.53 38.53 38.52

35.11 35.12

35.25 35.25

36.02 36.02 36.02 36.02

36.14 36.14 36.13 36.13

34.35 34.35 37.58 37.5735.01 35.01 38.48 38.48 38.47

35.13 35.13

35.45 35.45

35.50 35.50 35.50 35.50

36.03 36.03 36.03 36.03

36.22 36.21

34.33 34.33 37.56 37.56

35.10 35.10 38.47 38.47 38.46

35.40 35.40

35.45 35.45

35.59 36.00 35.59 35.59

36.20 36.20


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4 4 4 5 5 5 5 1 1

HpCDD (S) NCDPE PFK OCDD OCDD DCDPE PFK TCDF TCDF

M+4 M+4 LOCK M+2 M+4 M+4 LOCK M M+2

437.8140 479.7165 430.9728 457.7377 459.7348 513.6775 442.9728 303.9016 305.8987

40.33 45.00 45.00 31.06 31.06

38.47 42.39 42.39 28.45 28.45

42.38 42.38 25.26 25.26

38.46 28.45 28.45

29.07 29.06

42.46 42.46 25.27 25.26

38.52 27.48 27.49

28.46 28.45

29.07 29.06

30.50 30.49

42.40 42.40 25.27 25.2638.47 27.20 27.21

27.48 27.48

28.45 28.45

29.06 29.06

30.50 30.49

42.38 42.38 25.25 25.25

38.46 27.20 27.20

27.47 27.47

28.44 28.45

30.50 30.49


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1 1 2 2 2 2 3 3 3 3 4

TCDF (S) TCDF (S) PeCDF PeCDF PeCDF (S) PeCDF (S) HxCDF HxCDF HxCDF (S) HxCDF (S) HpCDF

M M M+4 M+2 M+2 M+4 M+2 M+4 M M+2 M+2

315.9419 317.9389 341.8567 339.8597 351.9000 353.8970 373.8208 375.8178 383.8639 385.8610 407.7818

31.04 31.04 33.37 33.37 33.36 36.27 36.27 36.26 36.26 39.17

34.13 34.13 34.13 36.56 36.57 36.56 36.56 41.17

37.45 37.44 37.44 37.44

28.44 28.44 33.18 33.18 33.17 33.17 ??? ??? 35.02 35.02 37.40

32.32 32.32 32.32 32.32 35.08 35.08 35.08 35.08 39.23

35.36 35.36

33.07 33.07 33.07 33.07 36.17 36.17

31.55 31.56 34.11 34.11 37.40

28.43 28.43 32.01 32.01 34.18 34.18 38.07

32.20 32.20 34.41 34.41

32.32 32.32 32.32 32.32 35.03 35.02 35.02 35.02

32.40 32.40 35.35 35.35

33.07 33.07 33.07 33.06 35.08 35.07

32.01 32.01 34.10 34.10 37.47

32.22 32.22 34.19 34.18 38.13

32.33 32.33 32.34 32.33 34.27 34.27

32.42 32.42 34.41 34.41

33.10 33.09 33.10 33.10 34.51 34.51

30.54 30.53 35.16 35.15 35.15 35.15

35.54 35.54

35.21 35.21

31.59 31.59 34.12 34.12 37.4032.20 32.20 34.19 34.19 38.08

32.32 32.32 32.31 32.31 34.34 34.34

28.44 28.44 32.41 32.40 34.42 34.43

33.03 33.03 35.05 35.05 35.04

33.07 33.07 33.07 33.07 35.10 35.10

30.53 30.53 35.39 35.39

36.19 36.19

31.58 31.59 34.10 34.10 37.39

32.13 32.13 34.17 34.17 38.06

32.19 32.19 34.40 34.40

28.43 28.42 32.32 32.32 35.02 35.02 35.01 35.01

33.07 33.07 33.06 33.06 35.06 35.06

30.51 30.51 35.35 35.35


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4 4 4 5 5 5 5

HpCDF HpCDF (S) HpCDF OCDF OCDF OCDD (S) OCDD (S)

M+4 M M+2 M+2 M+4 M+2 M+4

409.7788 417.8250 419.8220 441.7428 443.7399 469.7780 471.7750

39.17 39.16 39.16 44.59 44.59

41.17 41.17 41.17 45.19 45.18 44.59 44.59

37.40 37.39 37.39 42.54 42.54 42.38 42.38

39.23 39.22 39.22 42.38 42.38

37.40 37.39 37.39 42.38 42.40 42.37 42.37

38.06 42.53 42.53

39.22 39.22 42.37 42.37

37.46 37.46 37.46 42.45 42.45

38.13 42.47 42.47 42.45 42.45

39.27 39.27 43.01 43.01

37.40 37.39 37.39 42.41 42.41 42.39 42.3938.08 42.54 42.54

39.22 39.22

42.39 42.39

37.39 37.38 37.38 42.36 42.37 42.37

38.06 42.53 42.53

39.22 39.21


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Tab 4 - Estimated Detection Limit (EDL)

EDL [ug/L]= [(Hx1+Hx

2) x Qis x Df x 2.5]/[(His

1+His

2) x RRFn x W] Where:

Hx1

and Hx2

= peak height of noise for primary and secondary ana

Lab using a correction factor of 3 and not 2.5 - see SGS e-mail Qis = internal std. added (pg)

Note: Shaw calc. correct for G578-30 1,2,3,7,8-PeCDD (#2) - see e-mail Df = Dilution factor

Value of 3 used instead of 2.5 for consistency with SGS Lab Calculations Qrs = recovery std. added (pg)

His1

and His2

= peak height of noise for primary and secondary inte

RRFn = Relative Response Factor of analyte

W = Dry weight of sample extracted

SDG

Estimated Detection

Limit (EDL) Hx1

Hx2

(see

note) Qis Df His1

His2

RRFn W (g)

Wt.

before

drying

(g) % solids

SGS

Lab

Shaw (m

calculati

G578-29-1B 2,3,7,8-TCDD (#1) 3.87E+03 4.93E+03 3 2000 1 7.66E+06 9.74E+06 1.0087 9.32 11.48 81.2 0.323 0.32

G578-30-1B 2,3,7,8-TCDD (#1) 4.38E+03 5.42E+03 3 2000 1 7.61E+06 9.57E+06 1.0087 9.90 11.50 86.1 0.342 0.34

G578-30-1B 1,2,3,7,8-PeCDD (#2) 5.20E+03 5.54E+03 3 2000 1 1.90E+07 1.15E+07 1.0517 9.90 11.50 86.1 0.505 0.20

G578-33-1B 2,3,7,8-TCDD (#1) 3.70E+03 4.11E+03 3 2000 1 8.88E+06 1.13E+07 1.0087 8.97 10.54 85.1 0.257 0.25

G578-33-1B 1,2,3,7,8,9-HxCDF (#14) 6.07E+03 4.64E+03 3 2000 1 1.47E+07 2.69E+07 0.9220 8.97 10.54 85.1 0.558 0.18

G578-34-1B 2,3,7,8-TCDD (#1) 4.86E+03 5.10E+03 3 2000 1 8.71E+06 1.10E+07 1.0087 9.77 11.66 83.8 0.316 0.30

G578-34-1B 1,2,3,7,8,9-HxCDF (#14) 4.91E+03 5.24E+03 3 2000 1 1.30E+07 2.52E+07 0.9220 9.77 11.66 83.8 0.512 0.17

G578-35-1B 2,3,7,8-TCDD (#1) 5.13E+03 4.90E+03 3 2000 1 1.08E+07 1.38E+07 1.0087 10.36 11.72 88.4 0.235 0.23

G578-35-1B 1,2,3,7,8,9-HxCDF (#14) 7.12E+03 7.00E+03 3 2000 1 1.60E+07 2.96E+07 0.9220 10.36 11.72 88.4 0.482 0.19

G578-36-1B 2,3,7,8-TCDD (#1) 4.28E+03 4.73E+03 3 2000 1 9.25E+06 1.18E+07 1.0087 6.85 10.14 67.6 0.372 0.37

G578-36-1B 1,2,3,7,8,9-HxCDF (#14) 6.63E+03 6.51E+03 3 2000 1 1.32E+07 2.52E+07 0.9220 6.85 10.14 67.6 0.729 0.32


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Tab 5 - Estimated Maximum Possible Concentration (EMPC)

Where:

EMPC [ug/L]= [(Ax1+Ax

2) x Qis x Df]/[(Ais

1+Ais

2) x RRFis x W] Ax

1and Ax

2= area of primary and secondary interna

Qis = recovery std. added (pg)

Ais1

and Ais2

= area of primary and secondary recove

RRFis = Relative Response Factor of Internal S

Note: SGS Lab incorrectly calculated EMPC for 1,2,3,7,8-PeCDF (see e-mail) Qis = internal std. added (pg)

W = Dry weight of sample extracted

SDG Analyte Ax1

Ax2

Qis (pg) Df Ais1

Ais2

RRFnWt. beforedrying (g) % solids W (g)

SGSLab EMPC [ug/

G578-29-1B 1,2,3,7,8-PeCDD (#2) 2.66E+05 1.39E+05 2000 1 4.73E+07 2.97E+07 1.0517 11.48 81.20 9.32 1.080 1.073

G578-30-1B 1,2,3,4,6,7,8-HpCDF (#15) 5.74E+04 4.42E+04 2000 1 2.06E+07 4.67E+07 1.3752 11.50 86.10 9.90 0.222 0.222

G578-33-1B 1,2,3,7,8-PeCDF (#9) 7.71E+04 6.20E+04 2000 1 9.12E+07 5.75E+07 0.9873 10.54 85.10 8.97 0.212 0.211

G578-33-1B 2,3,4,6,7,8-HxCDF (#13) 1.18E+05 1.12E+05 2000 1 3.90E+07 7.38E+07 1.0650 10.54 85.10 8.97 0.426 0.427

G578-34-1B 1,2,3,7,8-PeCDF (#9) 5.01E+04 4.57E+04 2000 1 8.93E+07 5.62E+07 0.9873 11.66 83.8 9.77 0.137 0.137

G578-34-1B 2,3,4,6,7,8-HxCDF (#13) 7.34E+04 4.79E+04 2000 1 3.96E+07 7.54E+07 1.0650 11.66 83.8 9.77 0.203 0.203

G578-35-1B 1,2,3,7,8-PeCDF (#9) 9.52E+04 6.09E+04 2000 1 1.10E+08 6.99E+07 0.9873 11.72 88.4 10.36 0.596 0.170

G578-35-1B 1,2,3,7,8,9-HxCDF (#14) 4.12E+04 2.86E+04 2000 1 4.37E+07 8.22E+07 0.9220 11.72 88.4 10.36 0.116 0.116

G578-36-1B 1,2,3,7,8-PeCDF (#9) 3.52E+04 3.03E+04 2000 1 8.34E+07 5.21E+07 0.9873 10.14 67.6 6.85 0.143 0.143

G578-36-1B 2,3,4,7,8-PeCDF (#10) 9.08E+04 7.42E+04 2000 1 8.34E+07 5.21E+07 1.0154 10.14 67.6 6.85 0.350 0.350


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Tab 6 - Internal Standard % RecoveriesInternal Std. % Recovery (%Ris)= [(Ais +AiS ) x Qrs x 100]/[(Aris +Ars ) x RRFis x Qis]

Ais1

and Ais2

= area of primary and secondary internal st

Where: Qrs = recovery std. added (pg)

Ars1

and Ars2

= area of primary and secondary recovery

RRFis = Relative Response Factor of Internal Std.

Qis = internal std. added (pg)

SDG Analyte Ais1

(Ion 1) Ais2

(Ion 2) Qrs(pg) Ars1

(Ion 1) Ars2

(Ion 2) RRFis Qis(pg)

SGS (lab

calculation)

% Ris

manu

G578-29-1B13

C 2,3,7,8-TCDD (#18) 4.19E+07 5.30E+07 2000 4.53E+07 5.72E+07 1.1153 2 000 83.0 8

G578-29-1B13

C 1,2,3,7,8-PeCDD (#19) 4.73E+07 2.97E+07 2000 4.53E+07 5.72E+07 0.8155 2 000 92.1 9

G578-29-1B13

C 1,2,3,6,7,8-HxCDD (#20) 4.12E+07 3.39E+07 2000 4.76E+07 3.79E+07 1.0442 2 000 83.1 8

G578-29-1B13

C 1,2,3,4,6,7,8-HpCDD (#21) 3.06E+07 2.90E+07 2000 4.76E+07 3.79E+07 0.7878 2 000 88.5 8

G578-29-1B13

C OCDD (#22) 4.67E+07 5.23E+07 2000 4.76E+07 3.79E+07 0.6269 4 000 92.2 9

G578-30-1B13

C 2,3,7,8-TCDD (#18) 4.09E+07 5.19E+07 2000 4.66E+07 5.92E+07 1.1153 2 000 78.6 7

G578-30-1B13

C 1,2,3,7,8-PeCDD (#19) 4.87E+07 3.01E+07 2000 4.66E+07 5.92E+07 0.8155 2 000 91.3 9

G578-30-1B13

C 1,2,3,6,7,8-HxCDD (#20) 4.59E+07 3.65E+07 2000 4.93E+07 3.97E+07 1.0442 2 000 88.7 8

G578-30-1B13

C 1,2,3,4,6,7,8-HpCDD (#21) 3.06E+07 2.92E+07 2000 4.93E+07 3.97E+07 0.7878 2 000 85.4 8

G578-30-1B13

C OCDD (#22) 4.43E+07 4.96E+07 2000 4.93E+07 3.97E+07 0.6269 4 000 84.1 8

G578-33-1B13

C 2,3,7,8-TCDD (#18) 4.65E+07 5.90E+07 2000 5.39E+07 6.80E+07 1.1153 2 000 76.6 7

G578-33-1B13

C 1,2,3,7,8-PeCDD (#19) 5.50E+07 3.48E+07 2000 5.39E+07 6.80E+07 0.8155 2 000 90.4 9G578-33-1B

13C 1,2,3,6,7,8-HxCDD (#20) 4.90E+07 3.88E+07 2000 5.20E+07 4.14E+07 1.0442 2 000 90.1 9

G578-33-1B13

C 1,2,3,4,6,7,8-HpCDD (#21) 3.25E+07 3.08E+07 2000 5.20E+07 4.14E+07 0.7878 2 000 86.1 8

G578-33-1B13

C OCDD (#22) 5.19E+07 5.79E+07 2000 5.20E+07 4.14E+07 0.6269 4 000 93.7 9

G578-34-1B13

C 2,3,7,8-TCDD (#18) 5.02E+07 6.38E+07 2000 6.51E+07 8.21E+07 1.1153 2 000 69.5 6

G578-34-1B13

C 1,2,3,7,8-PeCDD (#19) 5.12E+07 3.24E+07 2000 6.51E+07 8.21E+07 0.8155 2 000 69.6 6

G578-34-1B13

C 1,2,3,6,7,8-HxCDD (#20) 5.67E+07 4.50E+07 2000 6.04E+07 4.82E+07 1.0442 2 000 89.6 8

G578-34-1B13

C 1,2,3,4,6,7,8-HpCDD (#21) 3.75E+07 3.53E+07 2000 6.04E+07 4.82E+07 0.7878 2 000 85.1 8

G578-34-1B13

C OCDD (#22) 6.02E+07 6.71E+07 2000 6.04E+07 4.82E+07 0.6269 4 000 93.4 9

G578-35-1B13

C 2,3,7,8-TCDD (#18) 5.95E+07 7.58E+07 2000 6.31E+07 7.96E+07 1.1153 2 000 85.0 8

G578-35-1B13

C 1,2,3,7,8-PeCDD (#19) 6.78E+07 4.28E+07 2000 6.31E+07 7.96E+07 0.8155 2 000 95.0 9

G578-35-1B13

C 1,2,3,6,7,8-HxCDD (#20) 5.50E+07 4.37E+07 2000 5.93E+07 4.73E+07 1.0442 2 000 88.6 8

G578-35-1B13

C 1,2,3,4,6,7,8-HpCDD (#21) 3.94E+07 3.72E+07 2000 5.93E+07 4.73E+07 0.7878 2 000 91.2 9

G578-35-1B13

C OCDD (#22) 6.42E+07 7.13E+07 2000 5.93E+07 4.73E+07 0.6269 4000 101.0 10

G578-36-1B13

C 2,3,7,8-TCDD (#18) 4.58E+07 5.79E+07 2000 4.48E+07 5.65E+07 1.1153 2 000 91.8 9


21/37

Tab 6 - Internal Standard % RecoveriesInternal Std. % Recovery (%Ris)= [(Ais +AiS ) x Qrs x 100]/[(Aris +Ars ) x RRFis x Qis]

Ais1

and Ais2

= area of primary and secondary internal sta

Where: Qrs = recovery std. added (pg)

Ars1

and Ars2

= area of primary and secondary recovery st

RRFis = Relative Response Factor of Internal Std.

Qis = internal std. added (pg)

SDG Analyte Ais

1

(Ion 1) Ais

2

(Ion 2) Qrs(pg) Ars

1

(Ion 1) Ars

2

(Ion 2) RRFis Qis(pg)

SGS (lab

calculation)

% Ris (

manua

G578-36-1B13

C 1,2,3,7,8-PeCDD (#19) 5.12E+07 3.25E+07 2000 4.48E+07 5.65E+07 0.8155 2000 101.0 101

G578-36-1B13

C 1,2,3,6,7,8-HxCDD (#20) 4.95E+07 3.93E+07 2000 4.91E+07 3.91E+07 1.0442 2000 96.4 96

G578-36-1B13

C 1,2,3,4,6,7,8-HpCDD (#21) 3.44E+07 3.29E+07 2000 4.91E+07 3.91E+07 0.7878 2000 96.8 96

G578-36-1B13

C OCDD (#22) 4.41E+07 4.91E+07 2000 4.91E+07 3.91E+07 0.6269 4000 84.3 84

G578-36-1B13

C 2,3,7,8-TCDF (#23) 7.57E+07 9.57E+07 2000 4.48E+07 5.65E+07 1.6529 2000 102.0 102


22/37

Tab 7 - First & Last Eluters (Spot Check)First & Last Isomer Eluters for each homologue of PCDD and PCDF

SDG Lab Sample ID Comment Instrument ID File Name

Sample

No.

Date

Acquired

Time

Acquired

Date

Processed

Time

Processed Name

First Eluter

(RT)

G578-29 RETCON S30-144H Ical WG17173 a07oct08a 1 7-Oct-08 15.46.35 8 -Oct-08 08.19.56 TCDD 28.35

G578-29 PeCDD 33.02

G578-29 HxCDD 35.48

G578-29 HpCDD 39.37

G578-29 OCDD 44.60

G578-29 TCDF 26.52G578-29 PeCDF 32.11

G578-29 HxCDF 35.22

G578-29 HpCDF 39.16

G578-29 OCDF 45.19

G578-29 RETCON S32-184L a30jun09a 1 30-Jun-08 16.43.56 1-Jul-09 07.02.23 TCDD 26.12

G578-29 PeCDD 32.02

G578-29 HxCDD 34.34

G578-29 HpCDD 37.57

G578-29 OCDD 42.39

G578-29 TCDF 24.30

G578-29 PeCDF 30.51

G578-29 HxCDF 34.11

G578-29 HpCDF 37.40

G578-29 OCDF 42.53

G578-29 RETCON S32-184L a30jun09a 15 1-Jul-09 04.00.27 1-Jul-09 07.02.30 TCDD 26.11

G578-29 PeCDD 32.01

G578-29 HxCDD 34.34

G578-29 HpCDD 37.57

G578-29 OCDD 42.38

G578-29 TCDF 24.30

G578-29 PeCDF 30.51

G578-29 HxCDF 34.11

G578-29 HpCDF 37.39

G578-29 OCDF 42.53

G578-29 RETCON S32-184L a02jul09 1 2-Jul-09 13.24.00 3-Jul-09 09.30.03 TCDD 26.13

G578-29 PeCDD 32.02

G578-29 HxCDD 34.35

G578-29 HpCDD 37.59

G578-29 OCDD 42.41


23/37

Tab 7 - First & Last Eluters (Spot Check)First & Last Isomer Eluters for each homologue of PCDD and PCDF

SDG Lab Sample ID Comment Instrument ID File Name

Sample

No.

Date

Acquired

Time

Acquired

Date

Processed

Time

Processed Name

First Eluter

(RT)

La

G578-29 TCDF 24.31

G578-29 PeCDF 30.52

G578-29 HxCDF 34.12

G578-29 HpCDF 37.41

G578-29 OCDF 42.55


24/37

Tab 8 - Total Dioxin/Furan Calculations

Note: see attached e-mail from SGS Laboratory regarding Totals Calculations

SDG Name (Totals)

Kept

(Y/N) RT Conc.

EDL (SDG

Lab)

Status

(SDG

Lab) SDG Lab

Peaks

(SDG

Lab)

Shaw

(manual) Comment

G578-29-1B Tetra-Dioxins 26.12 0.344 0.344 0.1361 ok

26.28 0.213 0.213 0.1361 ok

26.51 0.139 0.139 0.1361 ok

27.31 0.090 0.1361 S2N

27.46 0.291 0.291 0.1361 ok

28.00 0.143 0.1361 S2N

28.10 0.181 0.181 0.1361 ok

28.34 0.318 0.318 0.1361 ok

28.57 0.161 0.161 0.1361 ok

29.42 0.106 0.1504 S2N 2378-TCDD

30.01 0.118 0.118 0.1361 ok S2N (Manual Correc

30.43 0.089 0.1361 S2N

0.1504 EDL (2378-TCDD)

1.7650 8 Total EMPC Tetra-Di

1.7650 8 1.7650 Total Tetra-Dioxins

G578-29-1B Hepta-Furans 37.42 4.653 0.0994 ok

37.58 0.185 0.1116 ok 123678-HpCDF

38.07 7.056 0.1116 ok39.24 0.254 0.1274 ok 1234789-HpCDF

0.1274 0.1274 EDL (123478-HpCDF

12.1480 4 12.1480 Total EMPC Hepta-F

12.1480 4 12.1480 Total Hepta-Furans

G578-29-1B Tetra-Furans y 24.30 0.192 0.192 0.1498 ok

y 24.55 0.145 0.145 0.1498 ok

y 25.27 0.586 0.586 0.1498 ok

n 25.46 0.280 0.1498 EMPC

y 26.06 0.261 0.261 0.1498 ok

n 26.30 0.303 0.1498 EMPC

y 26.43 0.091 0.1498 S2N

n 27.12 0.168 0.1498 EMPC


25/37

Tab 8 - Total Dioxin/Furan Calculations

Note: see attached e-mail from SGS Laboratory regarding Totals Calculations

SDG Name (Totals)

Kept

(Y/N) RT Conc.

EDL (SDG

Lab)

Status

(SDG

Lab) SDG Lab

Peaks

(SDG

Lab)

Shaw

(manual) Comment

y 27.22 0.417 0.417 0.1498 ok

y 27.49 0.268 0.268 0.1498 ok

n 28.04 0.091 0.1498 S2N

n 28.16 0.105 0.1498 S2N

n 28.31 0.187 0.1498 EMPC

n 28.39 0.169 0.1498 EMPC

2378-TCDF n 28.46 0.366 0.1498 EMPC

y 29.07 0.359 0.359 0.1498 ok

n 30.52 0.198 0.1498 EMPC

0.1498 EDL (2378-TCDF)

2.2280 7 2.2280 Total Tetra-Furans

3.8990 14 Total EMPC Tetra-Fura


26/37

Tab 9 - Matrix Spike/Matrix Spike Duplicate %Recovery Recalculation(e.g. Ongoing Precision Results [OGP] / Ongoing Precision Duplicate Results [OGPD])

Where: %R = percent recovery

MS/MSD %R = (Spike Recovered - Sample original conc.) x 100 / Spike Added Spk rec. = Spike concentration recovered

% RPD = ABS(MS-MSD) x 100 / Average % recovery Spk add = Spike concentration added

Orig. conc. = Original sample concentration

SDG AnalyteSample(pg/uL)

Spiked (amt.

added)pg/uL

MS Recovery

(SGS Calc.)pg/uL

MS %

Recovery

(SGSCalc.)

MSD Recovery

(SGS Calc.)pg/uL

MSD %

Recovery(SGS Calc.)

MS % Recovery(Shaw manual)

MSD %

Recovery

(Shawmanual)

G578-29 2,3,7,8-TCDD (#1) 0.106 10 10.2 102 10.6 106.0 100.8 104.5

G578-29 1,2,3,7,8-PeCDD (#2) 0.501 50 48.8 97.6 48.2 96.4 96.6 95.4

G578-29 1,2,3,4,7,8-HxCDD (#3) 0.426 50 50.7 101 50.3 101.0 100.6 99.7

G578-29 1,2,3,6,7,8-HxCDD (#4) 1.259 50 48.0 95.9 46.9 93.8 93.4 91.3

G578-30 2,3,7,8-TCDD (#1) 0.342 10 9.9 99.4 10.1 101.0 96.0 97.6

G578-30 1,2,3,7,8-PeCDD (#2) 0.505 50 46.1 92.2 46.0 92.0 91.2 91.0

G578-30 1,2,3,4,7,8-HxCDD (#3) 0.505 50 45.7 91.4 45.3 90.5 90.4 89.6

G578-30 1,2,3,6,7,8-HxCDD (#4) 0.505 50 44.3 88.6 43.2 86.5 87.6 85.4

G578-33 2,3,7,8-TCDD (#1) 0.369 10 11.0 110 10.4 104.0 106.3 100.3

G578-33 1,2,3,7,8-PeCDD (#2) 0.563 50 49.0 98 48.5 97.0 96.9 95.9

G578-33 1,2,3,4,7,8-HxCDD (#3) 0.563 50 50.4 101 48.9 97.8 99.7 96.7

G578-33 1,2,3,6,7,8-HxCDD (#4) 0.563 50 48.6 97.3 48.9 97.8 96.1 96.7

G578-34 2,3,7,8-TCDD (#1) 0.316 10 10.3 103 10.5 105.0 99.8 101.8 G578-34 1,2,3,7,8-PeCDD (#2) 0.285 50 48.7 97.3 48.4 96.8 96.8 96.2

G578-34 1,2,3,4,7,8-HxCDD (#3) 0.516 50 48.2 96.5 49.1 98.2 95.4 97.2

G578-34 1,2,3,6,7,8-HxCDD (#4) 1.110 50 49.7 99.4 49.3 98.6 97.2 96.4

G578-35 2,3,7,8-TCDD (#1) 0.235 10 10.3 103 10.5 105.0 100.7 102.7

G578-35 1,2,3,7,8-PeCDD (#2) 0.521 50 48.7 97.3 48.4 98.6 96.4 95.8

G578-35 1,2,3,4,7,8-HxCDD (#3) 0.828 50 48.2 96.5 49.1 98.2 94.7 96.5

G578-35 1,2,3,6,7,8-HxCDD (#4) 1.070 50 49.7 99.4 49.3 98.6 97.3 96.5

G578-36 2,3,7,8-TCDD (#1) 0.372 10 10.4 104 10.7 107.0 100.3 103.3

G578-36 1,2,3,7,8-PeCDD (#2) 0.729 50 47.7 95.4 48.6 97.2 93.9 95.7

G578-36 1,2,3,4,7,8-HxCDD (#3) 0.292 50 45.5 91 49.0 98.0 90.4 97.4

G578-36 1,2,3,6,7,8-HxCDD (#4) 0.493 50 49.7 99.4 48.9 97.8 98.4 96.8


27/37

Tab 10 - Spot Check Relative Response Factor Calculations & Ion Ratios(RRF Calculation for unlabeled PCDDs/PCDFs &

13C-labeled Recovery Standard)

Response Factor (RRFn)= [(An1+An

2) x Qis]/[(Ais

1+Ais

2) x Qn]

Ion Ratio (RRFis) = [(Ais +Ais ) x Qrs]/[(Ars +Ars ) x Qis]

Where: An1

and An2

= areas of the primary and secondary ions of interest

Ais1

and Ais2

= area of primary and secondary internal standard ions

Ars1

and Ars2

= area of primary and secondary recovery standard ions

rs =13

C 1,2,3,4-TCDD (#27) is=13

C 1,2,3,7,8-P

rs =13

C 1,2,3,7,8,9-HxCDD (#28) is=13

C 1,2,3,6,7,8

Qn = quantity of unlabeled PCDD/PCDF analyte injected (pg)Qis = quantity of internal standard injected (pg)

Qn = quantity of recovery (e.g Injection) standard injected (pg)

SDG Name RRFn# RRFn (SDG Lab) RRFn (Shaw - manual) An1

An2

Qis Ais1

A

G578-29-1B 2,3,7,8-TCDD (#1) 1 1.06 1.0612 1.76E+05 2.40E+05 100 6.93E+07 8.7

2 0.99 0.9902 1.26E+06 1.58E+06 100 6.37E+07 7.9

3 0.99 0.9870 6.26E+06 8.22E+06 100 6.53E+07 8.1

4 1.01 1.0052 2.68E+07 3.46E+07 100 6.81E+07 8.4

5 1.00 1.0013 1.38E+08 1.79E+08 100 7.05E+07 8.7

Mean 1.0087 1.0090

%RSD 2.99

An1

An2

Qis Ais1

A

1,2,3,7,8-PeCDD (#2) 1 1.06 1.0595 8.52E+05 5.73E+05 100 6.61E+07 4.1

2 1.04 1.0421 6.65E+06 4.25E+06 100 6.41E+07 4.0

3 1.06 1.0544 3.44E+07 2.18E+07 100 6.52E+07 4.1

4 1.05 1.0481 1.45E+08 9.25E+07 100 6.95E+07 4.3

5 1.06 1.0558 7.85E+08 5.02E+08 100 7.46E+07 4.7

Mean 1.0517 1.0520

%RSD 6.26

RRFis (SDG Lab) RRFis (Shaw - manual) Ais1

Ais2

Qrs Ars1

A13

C 1,2,3,7,8,9-PeCDD 0.7882 0.7879 6.52E+07 4.14E+07 100 5.99E+07 7.513

C 1,2,3,6,7,8-HxCDD 1.0656 1.0665 6.18E+07 4.89E+07 100 5.79E+07 4.5


28/37


29/37

Tab 12 - Continuing Calibration Recalculation for Concentration of Unlabeled Compound

Where: An1

and An2


Qis = the concentration of the internal standard

Cc = [(An1+An

2) x Qis]/[(Ais

1+Ais

2) x RRFICAL] Ais

1and Ais

2= area of primary and secondary internal standard ions

%D= [(RRFICAL-RRFCCAL) x 100]/RRFICAL RRFCCAL = the response of the analyte from Continuing Calibration

RRFICAL = the response of the analyte from Initial Calibration

Cc = concentration of the unlabeled compound in the extract

Ct = concentration of the actual spiked compound%D = % Difference between RRFICAL & RRFCCAL

Analyte An1

(Ion 1) An2

(Ion 1) Qis Ais1

(Ion 1) Ais2

(Ion 2) RRFCCAL RRFICAL

Cc (SDG

Lab)

Ct (SDG

Lab)

%D

(SDG

Lab)

%D

(Shaw

manual)

Cc (Shaw

manual)

w/

RRFCCAL

G578-29 2,3,7,8-TCDD 9.12E+06 1.16E+07 100 8.90E+07 1.13E+08 1.0289 1.0087 10.200 10 2.00 2.0 9.97

G578-29 1,2,3,7,8-PeCDF 5.79E+07 3.74E+07 100 1.14E+08 7.25E+07 1.021 1.0517 48.540 50 -2.90 -3.0 50.05

G578-29 2,3,7,8-TCDF 1.27E+07 1.64E+07 100 1.36E+08 1.73E+08 0.9418 1.0368 9.084 10 -9.20 -10.1 10.00

G578-30 2,3,7,8-TCDD 1.05E+07 1.33E+07 100 9.59E+07 1.21E+08 1.0989 1.0087 10.890 10 -0.03 8.2 9.99

G578-30 1,2,3,7,8-PeCDF 9.39E+07 6.03E+07 100 1.96E+08 1.25E+08 0.9622 1.0517 48.730 50 -6.20 -9.3 49.92

G578-30 2,3,7,8-TCDF 1.61E+07 2.04E+07 100 1.56E+08 1.94E+08 1.0425 1.0368 10.060 10 -7.60 0.5 10.00

G578-33 2,3,7,8-TCDD 2.30E+07 2.93E+07 100 2.23E+08 2.83E+08 1.0341 1.0087 10.250 10 2.50 2.5 10.00

G578-33 1,2,3,7,8-PeCDF 1.97E+08 1.25E+08 100 4.02E+08 2.52E+08 0.9834 1.0517 49.800 50 -0.40 -6.9 50.07

G578-33 2,3,7,8-TCDF 3.41E+07 4.29E+07 100 3.33E+08 4.19E+08 1 .0241 1.0368 9.878 10 -1.20 -1.2 10.00

G578-34 2,3,7,8-TCDD 1.69E+07 2.18E+07 100 1.70E+08 2.16E+08 1.0042 1.0087 9.955 10 -0.40 -0.4 9.98

G578-34 1,2,3,7,8-PeCDF 1.47E+08 9.47E+07 100 3 .10E+08 1.98E+08 0.9508 1.0517 48.150 50 -3.70 -10.6 50.04

G578-34 2,3,7,8-TCDF 2.46E+07 3.15E+07 100 2.50E+08 3.13E+08 0.997 1.0368 9.161 10 -3.80 -4.0 9.99

G578-35 2,3,7,8-TCDD 1.69E+07 2.18E+07 100 1.70E+08 2.16E+08 1.0042 1.0087 9.955 10 -0.40 -0.4 9.98

G578-35 1,2,3,7,8-PeCDF 1.47E+08 9.47E+07 100 3 .10E+08 1.98E+08 0.9508 1.0517 48.150 50 -3.70 -10.6 50.04

G578-35 2,3,7,8-TCDF 2.46E+07 3.15E+07 100 2.50E+08 3.13E+08 0.997 1.0368 9.616 10 -3.80 -4.0 9.99

G578-36 2,3,7,8-TCDD 3.75E+06 4.73E+06 100 3.57E+07 4.52E+07 1.0485 1.0087 10.390 10 3.90 3.8 10.00

G578-36 1,2,3,7,8-PeCDF 2.95E+07 1.89E+07 100 6 .24E+07 3.96E+07 0.9487 1.0517 48.040 50 -3.90 -10.9 50.02

G578-36 2,3,7,8-TCDF 5.64E+06 7.18E+06 100 5.75E+07 7.12E+07 0 .9959 1.0368 9.606 10 -3.90 -4.1 10.00


30/37

Tab 13 - Spot Check Continuing Calibration Recalculation for Concentration of Labeled Compound

Where: Cc = concentration of the unlabeled compound in the extract

Ct = True Concentration

An1

and An2


Cc = [(An1+An

2) x Qis]/[(Ais

1+Ais

2) x RRFIs] Qis = the concentration of the internal standard

%D= [(RRFICAL-RRFCCAL) x 100]/RRFICAL Ais1

and Ais2

= area of primary and secondary internal standard ion

RRFIs = the response from the internal standard

RRFICAL = the response of the analyte from Initial Calibration

RRFCCAL = the response of the analyte from Continuing Calibration

%D = % Difference between RRFICAL & RRFCCAL

SDG CCAL Analyte An1

An2

Qis Ais1

Ais2

RRFIS RRFICAL RRFCCAL

Cc (SGS

Lab)

Ct (SGS

Lab)

Cc (Shaw

manual)

w/

RRFCCAL

%

(SG

La

G578-29 Initial37

CL 2,3,7,8-TCDD 2.10E+07 100 8.90E+07 1.13E+08 1.1097 1.1889 1.1592 9.750 10 9.37 -2

G578-29 Final37

CL 2,3,7,8-TCDD 2.60E+07 100 1.09E+08 1.39E+08 1.1036 1.1889 1.1406 9.594 10 9.50 -4

G578-30 Initial37

CL 2,3,7,8-TCDD 2.20E+07 100 9.41E+07 1.19E+08 1.1296 1.1889 1.1801 9.926 10 9.14 -3

G578-30 Final37

CL 2,3,7,8-TCDD 2.30E+07 100 9.59E+07 1.21E+08 1.1253 1.1889 1.2077 10.160 10 9.42 -2

G578-33 Initial

37

CL 2,3,7,8-TCDD 2.90E+07 100 1.23E+08 1.56E+08 1.1154 1.1889 1.1652 9.801 10 9.32 -1G578-33 Final

37CL 2,3,7,8-TCDD 5.20E+07 100 2.23E+08 2.83E+08 1.1193 1.1889 1.1516 9.724 10 9.18 -0

G578-34 Initial37

CL 2,3,7,8-TCDD 2.80E+07 100 1.19E+08 1.51E+08 1.1231 1.1889 1.1623 9.777 10 9.23 0

G578-34 Final37

CL 2,3,7,8-TCDD 3.90E+07 100 1.70E+08 2.16E+08 1.1434 1.1889 1.1713 9.852 10 8.84 1

G578-35 Initial37

CL 2,3,7,8-TCDD 2.80E+07 100 1.19E+08 1.51E+08 1.1231 1.1889 1.1623 9.777 10 9.23 2

G578-35 Final37

CL 2,3,7,8-TCDD 3.90E+07 100 1.70E+08 2.16E+08 1.1434 1.1889 1.1713 9.852 10 8.84 3

G578-36 Initial37

CL 2,3,7,8-TCDD 8.20E+06 100 3.46E+07 4.37E+07 1.0771 1.1889 1.1317 9.519 10 9.72 4

G578-36 Final37

CL 2,3,7,8-TCDD 8.30E+06 100 3.57E+07 4.52E+07 1.0767 1.1889 1.1058 9.301 10 9.53 5


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Tab 14 - Recalculation of Percent Difference for the RRFs for the Continuing Calibration V

% Difference (%D) = (RRFCCAL - RRFICAL) x 100 / RRFCCAL

Where:

RRFICAL = Relative Response factor from the initial calibration

RRFCCAL = Relative Response factor from the continuing calibration

(SDG lab) Shaw (manual)

SDG Analyte RRFCCAL RRFICAL %D %D

G578-29 2,3,7,8-TCDD (#1) 1.0058 1.0087 -0.3 -0.3

G578-29 1,2,3,7,8-PeCDD (#2) 1.0349 1.0517 -1.6 -1.6

G578-29 1,2,3,4,7,8-HxCDD (#3) 0.9240 0.9489 -2.6 -2.6

G578-29 1,2,3,6,7,8-HxCDD (#4) 0.9427 0.9916 -4.9 -4.9

G578-29 1,2,3,7,8,9-HxCDD (#5) 0.9430 0.9649 -2.3 -2.3

G578-29 1,2,3,4,6,7,8-HpCDD (#6) 1.0057 1.0612 -5.2 -5.2

G578-29 OCDD (#7) 1.0255 1.0643 -3.6 -3.6

G578-29 2,3,7,8-TCDF (#8) 0.9577 1.0368 -7.6 -7.6

G578-29 1,2,3,7,8-PeCDF (#9) 0.9262 0.9873 -6.2 -6.2

G578-29 2,3,4,7,8-PeCDF (#10) 0.9515 1.0154 -6.3 -6.3

G578-30 2,3,7,8-TCDD (#1) 1.0093 1.0087 0.1 0.1

G578-30 1,2,3,7,8-PeCDD (#2) 1.0110 1.0517 -3.9 -3.9

G578-30 1,2,3,4,7,8-HxCDD (#3) 0.9361 0.9489 -1.3 -1.3

G578-30 1,2,3,6,7,8-HxCDD (#4) 0.9457 0.9916 -4.6 -4.6

G578-30 1,2,3,7,8,9-HxCDD (#5) 0.9386 0.9649 -2.7 -2.7

G578-30 1,2,3,4,6,7,8-HpCDD (#6) 1.0428 1.0612 -1.7 -1.7

G578-30 OCDD (#7) 1.0437 1.0643 -1.9 -1.9

G578-30 2,3,7,8-TCDF (#8) 0.9600 1.0368 -7.4 -7.4

G578-30 1,2,3,7,8-PeCDF (#9) 0.9391 0.9873 -4.9 -4.9

G578-30 2,3,4,7,8-PeCDF (#10) 1.0044 1.0154 -1.1 -1.1

G578-33 2,3,7,8-TCDD (#1) 1.0341 1.0087 2.5 2.5

G578-33 1,2,3,7,8-PeCDD (#2) 1.0488 1.0517 -0.3 -0.3

G578-33 1,2,3,4,7,8-HxCDD (#3) 0.9328 0.9489 -1.7 -1.7

G578-33 1,2,3,6,7,8-HxCDD (#4) 0.9577 0.9916 -3.4 -3.4


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Where:





G578-33 1,2,3,7,8,9-HxCDD (#5) 0.9282 0.9649 -3.8 -3.8

G578-33 1,2,3,4,6,7,8-HpCDD (#6) 1.0534 1.0612 -0.7 -0.7

G578-33 OCDD (#7) 1.0593 1.0643 -0.5 -0.5

G578-33 2,3,7,8-TCDF (#8) 1.0241 1.0368 -1.2 -1.2

G578-33 1,2,3,7,8-PeCDF (#9) 0.9834 0.9873 -0.4 -0.4

G578-33 2,3,4,7,8-PeCDF (#10) 0.9766 1.0154 -3.8 -3.8

G578-34 2,3,7,8-TCDD (#1) 1.0195 1.0087 1.1 1.1

G578-34 1,2,3,7,8-PeCDD (#2) 1.0445 1.0517 -0.7 -0.7

G578-34 1,2,3,4,7,8-HxCDD (#3) 0.9430 0.9489 -0.6 -0.6

G578-34 1,2,3,6,7,8-HxCDD (#4) 1.0022 0.9916 1.1 1.1

G578-34 1,2,3,7,8,9-HxCDD (#5) 0.9935 0.9649 3.0 3.0

G578-34 1,2,3,4,6,7,8-HpCDD (#6) 1.0519 1.0612 -0.9 -0.9

G578-34 OCDD (#7) 1.0563 1.0643 -0.7 -0.8

G578-34 2,3,7,8-TCDF (#8) 1.0030 1.0368 -3.3 -3.3

G578-34 1,2,3,7,8-PeCDF (#9) 0.9689 0.9873 -1.9 -1.9

G578-34 2,3,4,7,8-PeCDF (#10) 1.0235 1.0154 0.8 0.8

G578-35 2,3,7,8-TCDD (#1) 1.0195 1.0087 1.1 1.1

G578-35 1,2,3,7,8-PeCDD (#2) 1.0445 1.0517 -0.7 -0.7

G578-35 1,2,3,4,7,8-HxCDD (#3) 0.9430 0.9489 -0.6 -0.6

G578-35 1,2,3,6,7,8-HxCDD (#4) 1.0022 0.9916 1.1 1.1

G578-35 1,2,3,7,8,9-HxCDD (#5) 0.9935 0.9649 3.0 3.0

G578-35 1,2,3,4,6,7,8-HpCDD (#6) 1.0519 1.0612 -0.9 -0.9

G578-35 OCDD (#7) 1.0563 1.0643 -0.7 -0.8

G578-35 2,3,7,8-TCDF (#8) 1.0030 1.0368 -3.3 -3.3


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Where:





G578-35 1,2,3,7,8-PeCDF (#9) 0.9689 0.9873 -1.9 -1.9

G578-35 2,3,4,7,8-PeCDF (#10) 1.0235 1.0154 0.8 0.8

G578-36 2,3,7,8-TCDD (#1) 1.0181 1.0087 0.9 0.9

G578-36 1,2,3,7,8-PeCDD (#2) 1.0174 1.0517 -3.3 -3.3

G578-36 1,2,3,4,7,8-HxCDD (#3) 0.9225 0.9489 -2.8 -2.8

G578-36 1,2,3,6,7,8-HxCDD (#4) 0.9465 0.9916 -4.5 -4.5

G578-36 1,2,3,7,8,9-HxCDD (#5) 0.9111 0.9649 -5.6 -5.6

G578-36 1,2,3,4,6,7,8-HpCDD (#6) 1.0211 1.0612 -3.8 -3.8

G578-36 OCDD (#7) 1.0300 1.0643 -3.2 -3.2

G578-36 2,3,7,8-TCDF (#8) 0.9769 1.0368 -5.8 -5.8

G578-36 1,2,3,7,8-PeCDF (#9) 0.9419 0.9873 -4.6 -4.6

G578-36 2,3,4,7,8-PeCDF (#10) 0.9780 1.0154 -3.7 -3.7


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Tab 15 - Toxic Equivalency Factors (TEF) "Spot Check"

SDG SEM Lab ID Sampled Extracted Analyzed

G578-29-1B SEM-015 9794676006 6/15/2009 6/29/2009 7/2/2009G578-30-1B SEM-017 9794971005 6/16/2009 7/5/2009 7/7/2009

G578-30-2B SEM-017 9794971005 6/16/2009 7/5/2009 7/7/2009

G578-33-1B SEM-029 9796518001 6/25/2009 7/7/2009 7/10/2009

G578-33-2B SEM-029 9796518003 6/25/2009 7/7/2009 7/10/2009

G578-33-3B SEM-029 9796518004 6/25/2009 7/7/2009 7/10/2009

G578-33-4B SEM-029 9796518005 6/25/2009 7/7/2009 7/10/2009

G578-34-1B SEM-035 9797431001 7/1/2009 7/8/2009 7/11/2009

G578-34-2B SEM-035 9797431002 7/1/2009 7/8/2009 7/11/2009

G578-35-1B SEM-031 9796885001 6/29/2009 7/8/2009 7/11/2009

G578-35-2C SEM-031 9796885002 6/29/2009 7/8/2009 7/11/2009

G578-36-1B SEM-034 9797191001 6/30/2009 7/23/2009 7/28/2009

G578-36-2B SEM-034 9797191004 6/30/2009 7/23/2009 7/28/2009

G578-36-3B SEM-034 9797191005 6/30/2009 7/23/2009 7/28/2009

G578-36-4B SEM-034 9797191006 6/30/2009 7/23/2009 7/28/2009

G578-36-5B SEM-034 9797191007 6/30/2009 7/23/2009 7/28/2009

Spot Check: G578-29-1B

Where: if [conc] = ND, then for ND=0.5 TEF WHO 2005 use [EDL] or [E

TEF WHO 2005

Value

ND = 0.5 (for

EDL or EMPC

values)

[Sample Conc.] x

WHO 2005 TEF EDL or EMPC

ND = 0 (forEDL or

EMPC

values)

2,3,7,8-TCDD 1 0.162 0.1615 0.323 0

1,2,3,7,8-PeCDD 1 0.269 0.2685 0.537 0

1,2,3,4,7,8-HxCDD 0.1 0.914 0.0914 0.914

1,2,3,6,7,8-HxCDD 0.1 2.7 0.27 2.7

1,2,3,7,8,9-HxCDD 0.1 2.48 0.248 2.48

1,2,3,4,6,7,8-HpCDD 0.01 64.1 0.641 64.1

OCDD 0.0003 2860 0.858 2860

2,3,7,8-TCDF 0.1 0.161 0.0161 0.322 0

1,2,3,7,8-PeCDF 0.03 0.352 0.01056 0.352

2,3,4,7,8-PeCDF 0.3 1.05 0.315 1.05

1,2,3,4,7,8-HxCDF 0.1 1.26 0.126 1.26

1,2,3,6,7,8-HxCDF 0.1 0.646 0.0646 0.646

2,3,4,6,7,8-HxCDF 0.1 0.951 0.0951 0.951

1,2,3,7,8,9-HxCDF 0.1 0.269 0.02685 0.537 0


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1,2,3,4,6,7,8-HpCDF 0.01 9.99 0.0999 9.99

1,2,3,4,7,8,9-HpCDF 0.01 0.545 0.00545 0.545

OCDF 0.0003 21.4 0.00642 21.4

3.30 TEQ [pg/g]

where ND=0.5


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WHO 2005 (ND=0)

EMPC [WHO

2005 TEQ

(ND=0)]

WHO 2005

TEQ

(ND=1/2)

EMPC [WHO 2005

TEQ (ND=1/2)]

[pg/g] [pg/g] [pg/g] [pg/g]

2.83 4.01 3.30 4.170.0895 0.0918 0.7530 0.7530

0.0568 0.0606 0.9180 0.9180

2.67 2.72 2.87 2.88

2.86 2.86 3.24 3.24

3.50 3.58 4.23 4.27

0.0979 0.1660 0.8340 0.8030

2.15 2.20 2.39 2.39

0.1730 0.2980 0.7390 0.6190

2.53 3.14 2.68 3.14

0.0167 0.0487 0.7900 0.8140

0.5770 0.6870 1.40 1.39

1.19 1.43 1.59 1.58

1.16 1.43 1.64 1.64

0.2190 0.2190 0.7780 0.7780

0.0478 0.0478 0.6790 0.6790

PCs] at for the ND= value

[Sample Conc.] x

WHO 2005 TEF

0

0

0.0914

0.2700

0.2480

0.6410

0.8580

0

0.0106

0.3150

0.1260

0.0646

0.0951

0


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0.0999

0.0055

0.0064

2.83 TEQ [pg/g]

where ND=0

method 8290 dioxin-furan template

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