MicroLiterLANALYTICAL SUPPLIES, INC.

888-232-7840 • Try the new www.MicroLiter.com • 770-932-6565PO Box 808, Suwanee, GA 30024

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In Less Than 4 HoursITSP Can Develop A Scaled Down Method With

Optimized Analytical Parameters

Step 1. Blend Solvents - The first macro set blends two solventsbeginning with 100% in position one decreasing to 0% in position 11.Likewise Solvent two starts with 0% in position 1 and ends with 100%in position 11.

MicroLiter has developed a 96 well microplateand a set of macros that automates methoddevelopment based on application notesprovided by most sorbent manufacturers. It isalso an excellent tool for scaling down methodsyou belive can increase the productivity of yourlab while at the same time reducing the costsof solvents, raw sample requirements andhazardous waste disposal. Here’s how:

Solvent

Solvent

Raw

SolventSample Load

SolventRaw

ITSP

Wash Solvent Blend Elution Solvent Blend

As illustrated the sample begins to elute at approximately 37% MeOH.At approximately 70% Methanol the sample elution is complete andboth parameters are set. One other note: One for the chromatogramswas produced using an ITSP device twice proving in situations wherechain-of custody are not factors, the device can be reused.

Step 2. SolventStrength - Thismacro simply loadsthe device withsample at incre-ments of 10µL untilbreak throughoccurs. In this case the blue food coloring begins to elute between 30and 40µL.

Steps 3 and 4 Sample Breakthrough and Sample Load Capacity -These steps are performs to either individually or simple step 4 byitself. If the sample is relatively clean direct injection without a washcan offer specific data for Sample Break Through. If the sample is

dirty it could damage the analytica column so the device may requirewashing before and step offer offers this option. These macrosillustrate the 10mg C8 sorbent had no issues loading the samplewithin the requirements of the assay.

Join us for a One Day Seminar on Tuesday at Pittcon for Detailed Instruction On Set-up and Operation of ITSP.The Morning session details conversion of your PAL to ITSP includinga detailed explanation of the ITSP device, installation of the ITSPHardware Kit and specific macros that assist in robust performance .

The Afternoon session details programming, method development,conversion to a fixed macro and integration into analytical instrumentoperating systems. Space is limited so call today to reserve your space.