Methods in Enzymology

Volume 154

Recombinant DNAPart E

Lawrence GrossmanRay Wu

CONTRIBUTORS TO VOLUME 154 ix

PREFACE Xiii

VOLUMES IN SERIES XV

Section I. Methods for Cloning cDN A

1. High-Efficiency Cloning of Full-Length cDNA ; H . OKAYAMA ,

Construction and Screening of cDNA Expres- M . KAWAICHI ,

sion Libraries for Mammalian Cells

M . BROWNSTEIN ,

F. LEE, T . YOKOTA ,

AND K . ARAI

3

2 . A Method for Cloning Full-Length cDNA in Plas- GISELA HEIDECKER AND

mid Vectors

JOACHIM MESSING

28

3 . An Efficient Vector-Primer cDNA Cloning DANNY C . ALEXANDER

4 1

System

4 . Use of Primer-Restriction End Adapters in cDNA CHRISTOPHER COLECLOUGH

64Cloning

Section II . Identification of Cloned Genes and Mapping of Genes

5 . Mapping of RNA Using S I Nuclease and Synthetic A . A . REYES AN D

Oligonucleotides

R. BRUCE WALLACE

8 7

6. Oligonucleotide Hybridization Techniques

C . GARRETT MIYAD A

AND R. BRUCE WALLACE

9 4

7. Agt 11 : Gene Isolation with Antibody Probes and MICHAEL SNYDER ,

Other Applications

STEPHEN ELLEDGE,

DOUGLAS SWEETSER ,

RICHARD A . YOUNG, AN D

RONALD W . DAVIS

107

8. Searching for Clones with Open Reading Frames MARK R . GRAY ,

GAIL P . MAZZARA ,

PRANHITHA REDDY ,

AND MICHAEL ROSBASH

129

9. Use of Open Reading Frame Expression Vectors GEORGE M . WEINSTOCK

156

10 . 5-Fluoroorotic Acid as a Selective Agent in Yeast JEF D . BOEKE ,

Molecular Genetics

JOSHUA TRUEHEART ,

GEORGES NATSOULIS, AND

GERALD R . FINK

164

11 . Transposon Tn5 Mutagenesis to Map Genes

FRANS J . DE BRUDN

17 5

12 . Site-Directed Tn5 and Transplacement Mutagene- JOHN D . Noll ,sis : Methods to Identify Symbiotic Nitrogen MITTUR N . JAGADISH ,

Fixation Genes in Slow-Growing Rhizobium

AND ALADAR A . SZALAY

197

Section III . Chemical Synthesis and Analysis of Oligodeoxynucleotide s

13 . Simultaneous Synthesis and Biological Applica- RONALD FRANK ,

Lions of DNA Fragments : An Efficient and ANDREAS MEYERHANS ,

Complete Methodology

KONRAD SCHWELLNUS, AN D

HELMUT BLOCKER

22 1

14 . The Segmented Paper Method : DNA Synthesis HANS W . DJURHUUS MATTRES ,

and Mutagenesis by Rapid Microscale "Shot- ADRIEN STAUB, AN D

gun Gene Synthesis "

PIERRE CHAMBON

250

15 . Chemical Synthesis of Deoxyoligonucleotides by M . H . CARUTHERS ,

the Phosphoramidite Method

A . D . BARONE ,

S . L . BEAUCAGE ,

D . R . DODDS, E . F . FISHER ,

L. J . MCBRIDE ,

M. MATTEUCCI ,

Z . STABINSKY, AN D

J .-Y . TANG

287

16 . An Automated DNA Synthesizer Employing De- SUZANNA J . HORVATH ,

oxynucleoside 3'-Phosphoramidites

JOSEPH R . FIRCA ,

TIM HUNKAPILIER ,

MICHAEL W . HUNKAPILLER,

AND LEROY HOOD

31 4

Section IV. Site-Specific Mutagenesis and Protein Engineering

17 . Oligonucleotide-Directed Mutagenesis : A Simple MARK J . ZOLLER AN DMethod Using Two Oligonucleotide Primers MICHAEL SMITH

329and a Single-Stranded DNA Template

18 . Oligonucleotide-Directed Construction of Muta- WILFRIED KRAMER AN D

tions via Gapped Duplex DNA

HANS-JOACHIM FRITZ

350

19 . Rapid and Efficient Site-Specific Mutagenesis THOMAS A . KUNKEL ,

without Phenotypic Selection

JOHN D . ROBERTS, AN D

RICHARD A . ZAKOUR

367

20 . Improved Oligonucleotide-Directed Mutagenesis PAUL CARTER

38 2Using M13 Vectors

21 . Site-Specific Mutagenesis to Modify the Human DAVID F . MARK ,

Tumor Necrosis Factor Gene

ALICE WANG ,

AND COREY LEVENSON

403

22 . An Improved Method to Obtain a Large Number RICHARD PINE

of Mutants in a Defined Region of DNA

AND P . C . HUANG

41 5

23 . Molecular Mechanics and Dynamics in Protein PETER KOLLMAN AN D

Design

W . F . VAN GUNSTEREN

43 0

24 . The Use of Structural Profiles and Parametric Se- S .ANDOR PONGOR

450quence Comparison in the Rational Design o f

'

Polypeptide s

25 . Structure-Function Analysis of Proteins through JOHN W . TAYLOR AN D

the Design, Synthesis, and Study of Peptide E. T . KAISER

47 3Model s

26 . Effect of Point Mutations on the Folding of Globu- C . ROBERT MATTHEWS

49 8lar Protein s

27 . Structure and Thermal Stability of Phage T4 Lyso- TOM ALBER AND

zyme

BRIAN W . MATTHEWS

51 1

AUTHOR INDEX 53 5

SUBJECT INDEX 555