micro pipette use age

8/8/2019 Micro Pipette Use Age

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Use of the Labnet Micropipettor


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Purpose of the Pipettor

Biotechnologists use a

pipettor to transfer

precise amounts of

liquids from one

container to another.

The measu

rements are inmicroliters 1 ml=1000 l.



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Care of the Pipettor

Misuse of the pipettor can

result in incorrect

measu

rements affectingyour investigation.

Do not drop the pipettor.

Do not adjust the volume

to less than 2 l.



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Parts of the Pipettor

1) yellow pushbutton

2) calibration knob

3) digital volume display

4) barrel

5) disposable pipette tip

6) tip ejector

3

5

6

1

4

6

1

62

3

4

5



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Volume AdjustmentStep 1

In the display window are

three vertical numbers.

The top number is tens l(10.0).

The middle number is

ones l (02.0).

The bottom number

tenths l (00.9).



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Volume AdjustmentStep 2

Set the pipettor to the

desired volume by turning

the calibration knob until

the volume display readsthe desired numbers.

7.5 l would read from

top to bottom in the

display window:0 - tens

7 - ones

.5 - tenths



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Transferring LiquidsStep 1

Adjust the pipettor to the

desired volu

me. Hold the pipettor so the

display is facing the user

and the thumb can easily

depress the yellowpushbutton.



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Open the box of yellow

pipette tips supplied by the

Office of Biotechnology.

Place the pipette cone (end)

into one of the tips and

press down firmly. NEVER USE THE

PIPETTOR WITHOUT A

TIP!



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Lift the pipettor and

attached tip away from the

tip box.

Depress the pushbutton to

the first stop before

placing the pipette tip inthe liquid.

Place the tip in the liquid.



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Slowly release the

pushbutton and draw the

liquid into the tip.

Remove the tip from the

liquid and visually check

for liquid in the tip.



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Place the tip into the

container which is to

receive the liquid. Slowly depress the

pushbutton as far as it can

go.

With the pushbutton fully

depressed, withdraw the

tip.



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Press down on the tip

ejector button to eject the

tip.

Dispose of the tip in an

appropriate container.


micro pipette use age

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