microbiology slides
TRANSCRIPT
Pharyngitis. Sputum. Plate- Strep. pyogenes on BA with bacitracin disc
• Describe –4m– Small dry round convex, pin point colonies– Clear zone of hemolysis (beta)– Bacitracin susceptible, growth prevented
• Antimicrobial –2m– Penicilin G– erythromycin
• 2 direct complications –2m– Otitis media– Sinusitis– Menigitis
• 2 indirect complications –2m– Rheumatic fever– Acute GN
UTI, Plate- MacConkey Escherechia coli
• Describe, organism, how to confirm –4m– Pink colonies- ferment lactose– IMViC reaction ++--, H2S -
• Why viable bacteria count –2m– Contamination and infection– Distal 1/3 urethra normal flora n feces
• Interpret result –2m– >10x5 infection.– Probable infection but require confirmation
• Source of organism –2m – GIT, normal flora
• 2 drugs –2m– Ampicilin– Cotrimoxazole
Lung problem, sputum, Ziehl Neelsen LM• Describe –3m
– Acid fast bacili, pink rod– Slightly curved– Mycolic acid in cell wall
• Organism –1m– Mycobacterium tuberculosis
• 3 antibiotics –3m– Isoniazid– Rifampicin– Pyrazinamide
• 3 other organs affected –3m– Kidney– Liver– Brain– Bones
• Medium for culture –1m– Lowenstein Jensen
Watery diarrhea, stool.Plate- Vibrio parahaemolyticus TCBS
• Describe– Blue green colonies (TCBS)
• Virulence factor– Enterotoxin– Invasion
• Source of infection– Contaminated seafood and water
• Treatment– Self-limiting– Water electrolyte replacement
• Name other microorganism that can cause same disease– Vibrio cholerae
Pneumonia, bronchial lavage. Plate- Stap. aureus
• Describe– Golden yellow (can be grey white) colonies on ordinary agar– Round convex moderate in size– Beta hemolysis, clear zone on BA
• Tests– Catalase +– Tube coagulase +– Mannitol fermentation +
• 2 virulence factors– Capsule– Exotoxin– Protein A at cell wall
• 3 complications– Pneumonia– Endocarditis– Septicemia– Septic arthritis
IVDU + tricuspid murmur, consolidation of lung. Yellow colonies on nutrient agar
• Specimen –2m– Sputum
• Describe, bacteria, confirmatory test –6m– Golden yellow colonies– Staphy. aureus– Catalase and coagulase test
• Drug –2m– Penicillin– Gentamicin– vancomycin
Stool, EM- rotavirus• Disease –2m
– Viral gastroenteritis
• Describe– Cubic symmetry– Outer capsid coat arranged like spokes of wheel
• 2 other virus –2m– Adenovirus– Coronavirus
• Age group patient –2m– Young children, 6-24 months
• 2 other diagnosis –2m– Serology ELISA– PCR
• Treatment and prevention –2m– Water electrolyte replacement– Live attenuated oral vaccine