MicropipettesMicropipettesMeasuring volumes which are Measuring volumes which are

what we scientists call what we scientists call ““very, very small”very, very small”

•Don’t set the pipettes past their intended range (high or low), even though you can.

•ALWAYS return the dial to the highest setting before putting the pipette away.

•ALWAYS return the pipette to its correct storage bin

MICROPIPETTE RULES

High Range: 100-1000 µL

Use large blue or white tips

Digits from top to bottom represent:

1000’s

100’s

10’sThe 1’s are indicated by the tiny white tick marks in the dial with the 10’s. Marks are in increments of 2 µL

This pipette is set to 1000 µL

This pipette is set to

998 µL

This pipette is set to 990 µL

Mid-range:

5.0-50.0 µL

10.0-100.0 µL, 20.0-200.0 µL

Range is printed on the button or on the side

Use medium sized yellow or white tips

10.0-100.0 µL

This pipette is set to its maximum:

100.0 µL

Digits represent:

100’s

10’s

1’s

Tick marks represent 0.1’s in increments of 0.2 µL

Low Range:

0.50-10.00 µL

2.00-20.00 µL

Digits represent:

10’s

1’s

0.1’sTick marks are 0.01’s in increments of 0.02 µL

Use small white tips

Rainin 0.50-10.00 µL pipettes use a unique tip

Clear tip has an extra wide mouth that surrounds and goes over the barrel and eject bar.

These tips can ONLY be used with the Rainin 0.5-10

•Feel the stops before you begin taking up or dispensing any liquid.

1st stop: Exact volume to which the pipette is set. Press to this stop before immersing pipette tip in the desired solution.

2nd stop: Use when dispensing solution. Pushes an extra volume of air to dislodge any solution adhering to the tip.

How to pipette

Great summary

•Extract and dispense SLOWLY.

•When dispensing, touch the tip to the side or bottom of the tube, or if there is liquid already in the tube, touch the tip to the liquid.

•When taking up or dispensing liquid, don’t put the tip any deeper into the liquid than necessary.

•NEVER hold pipette so that the tip is higher than the handle or liquid leaks into the barrel (BAD!)

•Always hold pipettes vertically when in use.

•Check to be sure the tip is on securely.

•Only open the tip box when getting a tip – DON’T LEAVE IT OPEN!