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MICROSCOPE NOTES

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MICROSCOPE NOTES

Types of Microscopes

1. Compound Light Microscope: allows light to pass through an image and use two lenses to magnify object

2. Electron Microscope: Uses beams of electrons to form an image Transmission

Electron Microscope (TEM)

Scanning Electron Microscope (SEM)

Page 3: MICROSCOPE NOTES. Types of Microscopes 1. Compound Light Microscope: allows light to pass through an image and use two lenses to magnify object 2

A. TEM- Shines a beam of light through a thin species to form image

B. SEM- Shines a narrow beam of electrons on the surface to form image

Page 4: MICROSCOPE NOTES. Types of Microscopes 1. Compound Light Microscope: allows light to pass through an image and use two lenses to magnify object 2

Page 5: MICROSCOPE NOTES. Types of Microscopes 1. Compound Light Microscope: allows light to pass through an image and use two lenses to magnify object 2

Parts of the microscope

Eyepiece: contains a lens capable of 10x magnification

Revolving Nosepiece: turns to change from one power to another

Low power objective: attached to the revolving nosepiece and contains a lens capable of 4x magnification

Page 6: MICROSCOPE NOTES. Types of Microscopes 1. Compound Light Microscope: allows light to pass through an image and use two lenses to magnify object 2

Medium power objective: attached to the revolving nosepiece and contains a lens capable of 10x magnification

High power objective: attached to the revolving nosepiece and contains a lens capable of 43x magnification

Diaphragm: regulates the amount of light entering the scope

Page 7: MICROSCOPE NOTES. Types of Microscopes 1. Compound Light Microscope: allows light to pass through an image and use two lenses to magnify object 2

Coarse Adjustment: brings objects into rapid but coarse focus

Fine Adjustment: brings objects slowly into fine focus

Page 8: MICROSCOPE NOTES. Types of Microscopes 1. Compound Light Microscope: allows light to pass through an image and use two lenses to magnify object 2

Mirror/lamp: directs light into the scope adjustment

Stage Clips: holds glass slide and specimen in place

Stage Opening: allows light to pass through the stage

Stage: supports slide

Page 9: MICROSCOPE NOTES. Types of Microscopes 1. Compound Light Microscope: allows light to pass through an image and use two lenses to magnify object 2

To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective lens.

We can see better detail with higher powers of magnification, but we cannot see as much of the image.

Page 10: MICROSCOPE NOTES. Types of Microscopes 1. Compound Light Microscope: allows light to pass through an image and use two lenses to magnify object 2

When using the microscope… 1-Turn on the microscope and then rotate

the nosepiece to click the red-banded objective into place.

2-Place a slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get the image into view and then use the fine adjustment knob (small knob) to make it clearer.

Page 11: MICROSCOPE NOTES. Types of Microscopes 1. Compound Light Microscope: allows light to pass through an image and use two lenses to magnify object 2

3- Once you have the image in view, rotate the nosepiece to view it under different powers. Draw what you see on your worksheet!

Be careful with the largest objective! Sometimes there is not enough room and you will not be able to use it!

4- When you are done, turn off the microscope and put up the slides you used.

Page 12: MICROSCOPE NOTES. Types of Microscopes 1. Compound Light Microscope: allows light to pass through an image and use two lenses to magnify object 2

How to make a wet-mount slide…

1- Get a clean slide and coverslip from your teacher. 2- Place ONE drop of water in the middle of the

slide. Don’t use too much or the water will run off the edge and made a mess!

3- Place the edge of the coverslip on one side of the water drop

4- Slowly lower the coverslip on top of the drop. 5- Place the slide on the stage and view it first with

the red-banded objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives.

Compound Light Microscope

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