microscopes compound light microscopes are delicate precision instruments. be gentle. ms...
DESCRIPTION
CARE AND USE NEVER force the controls. Use a clean, dry lens tissue on the glass surfaces. Lift by grasping the arm and the base. Set the microscope down gently. Ms O'Connell3TRANSCRIPT
![Page 1: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/1.jpg)
MICROSCOPES
Compound light microscopes are delicate precision
instruments. Be gentle.
Ms O'Connell 1
![Page 2: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/2.jpg)
Word Parts
• micro – very small • macro – large • scope – to see
Ms O'Connell 2
![Page 3: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/3.jpg)
CARE AND USE
• NEVER force the controls.• Use a clean, dry lens tissue
on the glass surfaces. • Lift by grasping the arm
and the base. Set the microscope down gently.
Ms O'Connell 3
![Page 4: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/4.jpg)
• Place the microscope away from the edge of the table.
• Don’t let the cord hang over the edge of the table.
• Slides are usually made of glass and may break. Be careful of sharp edges.
Ms O'Connell 4
![Page 5: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/5.jpg)
ALWAYS start by using the low-power objective.
BIG + BIG = BAD
Ms O'Connell 5
![Page 6: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/6.jpg)
VOCABULARY• Power of magnification:
The relative enlargement of the specimen when seen through the microscope. – The power of
magnification can be calculated by multiplying the power of the eye piece lens by the power of the objective lens.
Ms O'Connell 6
![Page 7: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/7.jpg)
Inversion: The reversal of the specimen image by the microscope lenses. A specimen that appears upside
down when being viewed is actually right-side up on the slide.
Moving the specimen to the right causes its image to move to the left. Likewise, moving it down causes it to move upward.
Ms O'Connell 7
![Page 8: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/8.jpg)
• Resolution (resolving power): The smallest distance separating two objects that allows them to be seen as two distinct things rather than as a single entity.
Ms O'Connell 8
![Page 9: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/9.jpg)
Resolution (Resolving Power)
Ms O'Connell 9
![Page 10: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/10.jpg)
• Working distance: The distance between the tip of the objective and the top of the cover glass on the slide. The higher the magnification the smaller the working distance.
Ms O'Connell 10
![Page 11: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/11.jpg)
• Depth of focus: The thickness of a specimen which may be seen in focus at one time. The greater the power of magnification the lesser the depth of focus.
Ms O'Connell 11
![Page 12: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/12.jpg)
Depth of Focus Fibers
Ms O'Connell 12
![Page 13: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/13.jpg)
Field of vision: The surface area which can be seen when looking through the light microscope. The area decreases with increasing power of magnification.
Ms O'Connell 13
![Page 14: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/14.jpg)
TYPES OF MICROSCOPES• Compound light
microscope– Glass lenses focus a beam
of light on the specimen– Used to view cells and
some organelles
Ms O'Connell 14
![Page 15: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/15.jpg)
• Dissecting microscope: – Also called a stereo
microscope– Used for macro
objects (e.g., bugs)
Ms O'Connell 15
![Page 16: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/16.jpg)
• Transmission electron microscope (TEM): Electrons pass through a thin sliced specimen.
Ms O'Connell 16
![Page 17: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/17.jpg)
• Scanning electron microscope (SEM): Electrons are directed at the surface of the specimen.
Ms O'Connell 17
Acanthus anther with pollen
![Page 18: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/18.jpg)
Ms O'Connell 18
![Page 19: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/19.jpg)
Ms O'Connell 19
![Page 20: MICROSCOPES Compound light microscopes are delicate precision instruments. Be gentle. Ms O'Connell1](https://reader033.vdocuments.net/reader033/viewer/2022051503/5a4d1acb7f8b9ab05996f692/html5/thumbnails/20.jpg)
Ms O'Connell 20