milk
DESCRIPTION
milkTRANSCRIPT
What is milk ?
• Normal mammary gland secretion of female mammals
• It is the first food for the baby mammaline
• Freezing point – 00 C(water) / -0.550C(solids)
RIBOFLAVIN Ca.caseinate Carotene&xanthophyl
TYPES OF MILKo Standardized milk: buffalo milk & skimmed milk ( fat -4.5% & SNF is 8.5%)
o Whole milk: 3.25% milk fat & 8.25% milk solids (50% of its calories 4m fat)
o Reduced-fat milk (2%): This milk contains 2% milk fat (35% of its calories)
o Low-fat milk (1%): 23% of its calories from fat
o Skimmed milk/non-fat milk: NMT 0.5% milk fat 5% of its calories from fat. Skimmed milk has about half the calories of whole milk.
o Pasteurized : milk kill bacteria(not spores)o Pasteurized milk will keep fresh for 2-3 days in a fridge
o Unpasteurized - raw or untreated milko It is recommended that babies, young children, the
elderly, pregnant women and anyone with an impaired immune system should avoid drinking unpasteurized milk.
630C for 30min 720C For 15sec
o Long - life - milk pasteurized & homogenized and then kept at a high temp for destroy bacteria.
odd burnt caramel flavour stored for 1 week
o UHT( ultra-heat treatment) milk heated at high temp (1320C / 2700F)
Stored for upto 3 months
o Dried Milk: in powdered form.
o Evaporated : homogenized milk with considerably reduced water content
o Condensed milk :simply evaporated milk to which sugar has been added to thicken and sweeten it. It is mainly used for making desserts and sweets.
Contamination of milk
THE NATIONAL NEWS(11-1-2012)& BBC: “68% of Indian milk contaminated’’diluted with water sweeteners Fat se volumenon-edible solidsglucose and skimmed milk powder
o Addition of water not only reduces the nutritional value of milk but contaminated water may also pose health risks
o The presence of detergent "indicates lack of hygiene and sanitation in the milk handling”
Preparation of sample
o Warm the sample to 37 0 – 40 0 C by transferring it to the
beaker & keep it in a water bath maintained at 400 - 450C
o Stir slowly for proper homogenisation. Mix sample thoroughly by pouring back into the bottle, mixing to dislodge any residual fat sticking to the sides and pour it back in the beaker.
o Allow the sample to come to room temperature (26 0 - 28 0 C) and withdraw immediately for analysis.
Determination of total solid:
o take a weight of crucible. o weigh 5 g of milk in a crucible
o put a crucible in a water bath until dryness.
o after complete dryness put the crucible in an oven, and weigh after cooling.
o determination the percent of total solid.
%Of total solid = (wt of crucible +sample) after drying – wt of crucible/ wt of sample * 100
RICHMOND’S Formula For Total Solids :
For cow milk 0.66Where, D = Density F = % Fat
T=0.25D+1.22F+0.72
DETERMINATION OF CHLORIDE CONTENT 10 ml milk + 40 ml water
add 10 drops of pot.chromate
Titrate with 0.1 N AgN03
1 ml of o.1 N AgN03 equivalent to 3.55mg of Cl-
Brick red ppt
INDICATIVE OF DISEASED STATE OF ANIMAL
TITRABLE ACIDITY
10 ml milk + 1 ml phnolpthalein indicator
Titrate with 0.1N NaOH 1 ml 0.1 N NaOH ≈ 0.009 g of lactic acid
Determination of Fat in Milk
Gerber Method:Principle:o milk +H2S04 + iso-amyl
alcohol o permitting dissoln of the
protein and release of fat. o The tubes are centrifuged
and the fat rising into the calibrated part of the tube is measured as percentage of the fat content of the milk sample
Procedure:
o 10 ml of H2S04 into a butyrometer tube & Mix the milk sample
o Add 1 ml of Amyl alcohol,close with a lock stopper
o shake until homogeneous soln.Keep in a water bath for 5 min at 65o C
o centrifuge for 4 min. at 1100 rpm.
o Remove the butyrometer tubes and place in water bath for 5 min.at 650C.
o Read the percentage of fat
Werner Schmidt Method(by Acid Digestion Method):
PRINCIPLE:
o Milk proteins are digested with conc. HCl
o Liberated fat is extracted with alcohol, ethyl ether & petroleum ether
o Ethers are evaporated o Residue left behind is weighed to calculate the fat
content.
10 g milk+10 ml conc.HCl
stir with a glass rod until the contents turn dark brown
Mojonnier fat extraction flask 10 ml of C2H50H+ 25 ml of ethyl ether 25 ml of petroleum ether Shake vigorously for 1 min
Centrifuge Mojonnier flask at about 600 rpm
heat on a Bunsen burner
cool to room temp
Shake vigorously for 1 min
Calculation: 100 (W1 - W2) Fat, percent w/w ---------------------- W3Where• W1 = Wt in g of contents in the flask before removal of fat.
• W2 = Wt in g of contents in the flask after removal of fat
• W3 = Wt in g of material taken for the test.
Detection of Cane Sugar in MilkModified Seliwanoff Method:PRINCIPLE:Fructose + resorcinol in HCl red colourprocedureProcedure: milk + conc. HCl filter 1 ml filtered milk serum & 5 ml modified resorcinol - HCl reagent
Withdraw the tube &observe the colour
red colour
std for 10 min
water bath for exactly 1 min
Test for QAC (Detergents)o To a centrifuge tube add 1 ml milk, 5 ml water, 1 ml EOSIN
soln & 0.2 ml buffer and shake hard for 10 sec.
o Centrifuge for 5 min at 3200 rpm.
o If QAC is present the bottom layer assumes a red or pink colour.
o Samples containing 1 mg / kg of QAC show a faint pink
o If the colour is deep pink or red, the amt of QAC can be approx. determined by titration with a std anionic detergent soln
Detection of added Urea in Milk
o 5 ml of milk is mixed with 5 ml of 1.6 % of p –Dimethyl amino benzaldehyde (DMAB) is added observed in milk containing added urea.
o The control (normal milk) shows a slight yellow
colour due to presence of natural urea.o :
Distinct yellow colour
Estimation of Urea in MilkPrepn of standard Curve:o Pipette 5 ml std solns into 25 ml T.T Add 5 ml
DMAB soln to each.
o Prepare reagent blank of 5 ml buffer and 5 ml DMAB soln. Shake tubes thoroughly and let stand for 10min.
o Read a@420 nm
Preparation of sample:o 10 ml of milk sample add 10 ml of Trichloro
acetic acid (TCA) to ppt the proteins and filtered.
o 5 ml of filtrate + 5 ml of DMABo The optical density of the yellow colour is
measured @ 420 nm. o From standard curve the amount of urea in
milk is calculated.
70 mg per 100 ml (700 ppm)
Hehner’s Test (HCHO): 2 ml milk in T.T
2 ml of 90 % H2SO4 traces of FeCl3
Formaldehyde
purple color ring at the junction
Test for presence of Salicylic acid: 50ml milk + 5 ml of dil.HCl + 50 ml ether
Wash ether layer with water
evaporate ether
1 drop of 0.5 % (v/v) FeCl3
Violet colour
Test for presence of H2O2
Milk + conc.HCl Mix well drop of HCHO soln 600C place starch-Iodine paper into soln
oxidesation of iodine
BLUE COLOR
Normal flora of milk:
Bacteriological Examination of Milk
oEnterococcus faecalisoStreptoccus lactusoLactobacillus sp.o Candida albicans (yogurt)
Determination of viable bacterial count :
The pour plate method: After preparation of 10 fold serial dilution from
the milk sample with ringer solution
Using 10 fold serial dilution methodViable Bacterial Count
9 ml Saline1 2 3Milk sample
1 ml milk 1 ml 1 ml
1/101/10 x 1/10
1/1001/100 x 1/10
1/1000
1 ml 1 ml 1 mlMelted NA
1 2 3
Results:
Dilution
factor 1 2 3 XX . y
10 x1 X1.y1
102 x2 X2.y2 103
x3 X3.y3
Viable Bacterial Count
No. of colonies per plateY
No. of cells per 1 ml = X1.y1 + X2.y2 + X3.y3
3
Results:
o Permissible number of bacterial flora in pasteurized milk is 5 x 104 cfu/ml
o Permissible number of bacterial flora in long life milk is 10 cfu/ml
Methylene Blue Reduction Test:
o Increasing the number of bacterial flora will reduce the colour of methylene blue more rapidly due to increasing consumption of oxygen.
o i.e: The speed of reduction of methylene blue colour is directly proportional to the number of bacteria present in milk sample.
determine quality of the milk
Results: The shorter the decolorization time, the higher
the number of bacterial flora present in milk, and the poor quality of milk
Decolorization time Result
30 min – 2 hrs Poor quality
2 – 6 hrs fair quality
6 – 8 hrs good quality
Over 8 hrs excellent quality
Test for coliforms:
o Done by inoculation of MacConkey’s broth with 0.1 ml of milk sample.
o Examine for the production of acid detected by changing the color of the medium from purple to yellow.