MIT’s Eau d’e coli iGEM 2006

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By: Robert Ovadia

BackgroundBackground

System Design

Results

What I Liked and Disliked

What makes a scent?

An enzyme that converts something that DOESN’T smell, into something that DOES smell.

JMT

jasmine

CCMT

cinnamon

BAMT

fresh / floral

BSMT

wintergreen

ATF1

banana

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BackgroundBackground

System Design

Results

What I Liked and Disliked

BSMT

wintergreen

ATF1

banana

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System Design

QuickTime™ and aTIFF (LZW) decompressor

are needed to see this picture.

BackgroundSystem Design• WGD• BGD• SAGD• IAGD• pOsmY + InverterResultsWhat I Liked and Disliked

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System Design

QuickTime™ and aTIFF (LZW) decompressor

are needed to see this picture.

Simple System

BackgroundSystem Design• WGD• BGD• SAGD• IAGD• pOsmY + InverterResultsWhat I Liked and Disliked

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System Design

Complex System

QuickTime™ and aTIFF (LZW) decompressor

are needed to see this picture.

BackgroundSystem Design• WGD• BGD• SAGD• IAGD• pOsmY + InverterResultsWhat I Liked and Disliked

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More Complex System

QuickTime™ and aTIFF (LZW) decompressor

are needed to see this picture.

BackgroundSystem Design• WGD• BGD• SAGD• IAGD• pOsmY + InverterResultsWhat I Liked and Disliked

System Design

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Complete System

QuickTime™ and aTIFF (LZW) decompressor

are needed to see this picture.

BackgroundSystem Design• WGD• BGD• SAGD• IAGD• pOsmY + InverterResultsWhat I Liked and Disliked

System Design

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System DesignWintergreen Generating Device (WGD)

QuickTime™ and aTIFF (LZW) decompressor

are needed to see this picture.

BackgroundSystem Design• WGD• BGD• SAGD• IAGD• pOsmY + InverterResultsWhat I Liked and Disliked

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System DesignBanana Generating Device (BGD)

QuickTime™ and aTIFF (LZW) decompressor

are needed to see this picture.

BackgroundSystem Design• WGD• BGD• SAGD• IAGD• pOsmY + InverterResultsWhat I Liked and Disliked

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System DesignSalicylic Acid Generating Device (SAGD)

QuickTime™ and aTIFF (LZW) decompressor

are needed to see this picture.

BackgroundSystem Design• WGD• BGD• SAGD• IAGD• pOsmY + InverterResultsWhat I Liked and Disliked

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System DesignIsoamyl Alcohol Generating Device (IAGD)

QuickTime™ and aTIFF (LZW) decompressor

are needed to see this picture.

BackgroundSystem Design• WGD• BGD• SAGD• IAGD• pOsmY + InverterResultsWhat I Liked and Disliked

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System DesignOsmY + Inverter

BackgroundSystem Design• WGD• BGD• SAGD• IAGD• pOsmY + InverterResultsWhat I Liked and Disliked

osmY: active in stationary phase & under high osmotic pressure conditions

osmY

osmY + inverter13

ResultsWintergreen Generating Device

BackgroundSystem DesignResults• WGD• Growth Dependent

Regulation• Growth Dependent

Regulation + Inverter• Indole Free Bacteria• Smell TestWhat I Liked and Disliked

O

O

OH

methyl salicylateMW = 152no methyl

salicylate [no peak at 9.1 minutes]

E. coli E. coli + precursor (SA) + WGD

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ResultsGrowth Dependent Regulation

BackgroundSystem DesignResults• WGD• Growth Dependent

Regulation• Growth Dependent

Regulation + Inverter• Indole Free Bacteria• Smell TestWhat I Liked and Disliked

0E+0

2E+4

4E+4

6E+4

8E+4

1E+5

1E+5

1E+5

2E+5

2E+5

2E+5

0.0 0.1 0.2 0.3 0.4 0.5

Absorbance 600nm (relative units)

GFP Signal (relative units)

BBa_J45995 (pOsmY-GFP)

BBa_J45017 (Negative control)

BBa_I7100 (Constitutive promoter-GFP)

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ResultsGrowth Dependent Regulation + Inverter

BackgroundSystem DesignResults• WGD• Growth Dependent

Regulation• Growth Dependent

Regulation + Inverter• Indole Free Bacteria• Smell TestWhat I Liked and Disliked

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ResultsIndole-Free Bacteria

BackgroundSystem DesignResults• WGD• Growth Dependent

Regulation• Growth Dependent

Regulation + Inverter• Indole-Free Bacteria• Smell TestWhat I Liked and Disliked

No indole [no peak at 10

minutes]

IndolePeak at 10 minutes

MW = 117

wild type E. coli chassis: tnaA5- E. coli

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ResultsSmell Test

BackgroundSystem DesignResults• WGD• Growth Dependent

Regulation• Growth Dependent

Regulation + Inverter• Indole-Free Bacteria• Smell TestWhat I Liked and Disliked

0

10

20

30

40

50

60

70

80

90

100

A (Mint) B (Stinky) C (Banana)

Percentage

Banana

Mint

Stinky

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What I Liked

BackgroundSystem DesignResultsWhat I Liked and Disliked• Likes

•Able to change a simple experiment into a more complicated experiment

•They looked at what MORE there was to do; took their experiment to the ‘next level’ EX: Simple System to Complete System

•Got the audience involved, EX: Smell test

•Easy to comprehend

•Perfect example of a successful iGEM project

Likes

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Sources

MIT’s iGEM PPT Presentation 2006.ppt (PowerPoint)

MIT’s iGEM PPT Presentation 2006.mov (Movie)

http://openwetware.org/wiki/IGEM:MIT/2006