model of dna dna is comprised of four base pairs
DESCRIPTION
Model of DNA DNA is Comprised of Four Base Pairs. DNA Restriction Enzymes. •Evolved by bacteria to protect against viral DNA infection •Endonucleases = cleave within DNA strands •Over 3,000 known enzymes. Enzyme Site Recognition. Restriction site. Palindrome. - PowerPoint PPT PresentationTRANSCRIPT
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Model of DNA
DNA is Comprised of Four Base Pairs
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DNA Restriction Enzymes
• Evolved by bacteria to protect against viral DNA infection
• Endonucleases = cleave within DNA strands
• Over 3,000 known enzymes
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Enzyme Site Recognition
• Each enzyme digests (cuts) DNA at a specific sequence = restriction site
• Enzymes recognize 4- or 6- base pair, palindromic sequences (eg GAATTC)
Palindrome
Restriction site
Fragment 1 Fragment 2
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5 vs 3 Prime Overhang
• Generates 5 prime overhang
Enzyme cuts
5’GGGCCC3’3’CCCGGG5’
5’GGG CCC3’3’CCC GGG5’
Extremos romos
(Extremos 5’ salientes)
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Common Restriction Enzymes
EcoRI– Eschericha coli– 5 prime overhang
Pstl– Providencia stuartii– 3 prime overhang
5’CTGCAG3’3’GACGTC5’
5’CTGCA3’ G3’3’G ACGTC5’
Extremos 3’ salientes
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DNA Fingerprinting Real WorldApplications • Crime scene
• Human relatedness
• Paternity
• Animal relatedness
• Anthropology studies
• Disease-causing organisms
• Food identification
• Human remains
• Monitoring transplants
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Workshop Time Line
• Restriction digest of DNA samples
• Introduction to DNA Fingerprinting and RFLP analysis
• Electrophoresis on Agarose gels
• Analysis and interpretation of results
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DNA FingerprintingProcedureOverview
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DNA Fingerprinting ProceduresDay One
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DNA Fingerprinting ProceduresDay Two
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DNA Fingerprinting ProceduresDay Three
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