mold growth prediction with biohygrothermal model · neurospora sitophila pen. bervicompactum pen....

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Mold growth prediction with Biohygrothermal Model

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Page 1: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

Mold growth prediction with

Biohygrothermal Model

Page 2: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

2

Contents:

Mold problems in practice

Growth Conditions

Steady State Model

Dynamic Model

Validation

Mold growth prediction with Biohygrothermal model

Page 3: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

3

Mould on a thermal bridge

(concrete header joist)

Mold problems in practice

Page 4: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

4

Mould at corners and edges

Mold problems in practice

Page 5: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

5

Mould behind

interior

insulation slabs

(EPS)

Mold problems in practice

Page 6: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

6

Growth conditions

Page 7: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

7

Absidia glauca

Alternaria alternata

Alternaria sp.

Asp. amstelodami

Asp. candidus

Asp. chevalieri

Asp. flavus

Asp. fumigatus

Asp. nidulans

Asp. niger

Asp. penicillioides

Asp. repens

Asp. restrictus

Asp. ruber

Asp. terreus

Asp. versicolor

Aureobasidium pullulans

Botrytis cinera

Cla. cladosporioides

Fusarium culmorum

Fusarium oxysporum

Mucor plumbeus

Neurospora sitophila

Pen. bervicompactum

Pen. chrysogenum

Pen. citrinum

Pen. cyclopium

Pen. expansum

Pen. frequentans / glabrum

Pen. glabrum

Pen. italicum

Rhizopus stolonifer

Scopulariopsis brevicausalis

Stachybotrys atra

Trichoderma viride

Trichothecium roseum

Wallemia sebi

0 10 20 40 50 6030

Temperatur [°C]

Temperature range and

optimum for mould growth

Growth conditions

Page 8: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

8

Relative Humidity [%]

Gro

wth

Ra

te µ

m/h

Humidity range for growth

of different mold species

Growth conditions

Page 9: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

9

Aspergillus restrictus (Smith) Hygrothermal Iso-lines

for germination time

and growth rate of a

typical mould species

Growth conditions

Page 10: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

10

Different Species

Aspergillus restrictus and other species

Growth conditions

Page 11: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

11

Potentially hazardous mold species

need higher humidity to germinate

Re

lati

ve

Hu

mid

ity [

%]

Temperature [°C] Critical species: Aspergillus fumigatus Aspergillus flavus

Stachybotrys chartarum

Growth conditions

Page 12: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

12

IBP mold growth test set-up:

Observation of the growth of a

typical mold cocktail on

different building materials

Growth conditions

Page 13: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

13

Substrate Classification

II non biodegradable

building materials

I biodegradable building

materials

0 optimum substrate

Re

lati

ve

hu

mid

ity

[%

]

70

75

80

85

90

95

100 Lowest Isopleth for MoldDifferent species

00 55 1010 1515 2020 2525 3030

Temperature [°C]Temperature [°C]

Re

lati

ve

hu

mid

ity [

%]

70

75

80

85

90

95

100 Lowest Isopleth for MoldDifferent species

00 55 1010 1515 2020 2525 3030

Temperature [°C]Temperature [°C]

Growth conditions

Page 14: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

14

Re

lati

ve

hu

mid

ity

[%

]

70

75

80

85

90

95

100 Lowest Isopleth for MoldDifferent species

00 55 1010 1515 2020 2525 3030

Temperature [°C]Temperature [°C]

Re

lati

ve

hu

mid

ity [

%]

70

75

80

85

90

95

100 Lowest Isopleth for MoldDifferent species

00 55 1010 1515 2020 2525 3030

Temperature [°C]Temperature [°C]

IEA-Annex 14 &

German Standard

DIN 4108: 80 %

Comparison

of LIM with

literature data

Growth conditions

Page 15: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

15

Steady-state conditions

R 20

Steady State Model

Page 16: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

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Steady State Model

Page 17: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

17

0

1

2 3

4

5

6

Steady state model for transient conditions ?!

Rela

tive H

um

idit

y [

%]

Fu

ng

al G

row

th [

mm

]

Transient conditions

Temperature [°C] Time [d]

Growth rate

Steady State Model

Page 18: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

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Dynamic model for

transient conditions

Dynamic Model

Page 19: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

19

Water retention curve Spore wall permeance

Model spore

Dynamic Model

Page 20: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

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Definition of critical spore water content for germination

Dynamic Model

Page 21: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

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Factor Real life WUFI-Bio

Humidity &

Temperature

determining factors critical water content = f (φ,θ)

Time strong influence germination, growth rate = f(t)

Substrate availability of nutrients,

(concentration of growth

inhibiting chemicals)

LIM 0, LIM 1, LIM 2

material specific isopleths

pH-Value high pH is toxic not accounted for

Spore dissemination spores are ubiquitous in air spores are assumed to be present

Lethal conditions high and low temp. or low RH

may be lethal

decline not yet accounted for

>> evaluation should be confined to

12 month starting anytime within

the simulated period

Model assumptions of WUFI-Bio

Dynamic Model

Page 22: Mold growth prediction with Biohygrothermal Model · Neurospora sitophila Pen. bervicompactum Pen. chrysogenum Pen. citrinum Pen. cyclopium Pen. expansum Pen. frequentans / glabrum

Mold growth prediction with

Biohygrothermal Model