molecular biology - aurogene · molecular biology catalog 2017 alpha-select silver efficiency cat...

Molecular

Biology

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Molecular Biology Molecular Biology techniques are widely spread in almost every laboratory. We provide reliable manufacturers of reagents and kits with the only goal to satisfy our customers needs. From cloning to NGS services, from micro RNA profiling to custom oligos synthesis,

we can bring solutions to your everyday research activity.

Cloning

Nucleic Acid Purification

End-Point PCR

Real Time PCR

RNA Single Molecule FISH

Custom Oligos and Probes

Exosomes

Molecular Biology Miscellaneous Reagents

Custom Services (Sequencing, Genome Editing..)

Molecular biology CATALOG 2017

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CLONING

Cloning In this section, we propose a selection of cloning reagents and kits. Quality and

money-saving, all at once!

Bioline provides a quick –stick ligase as well as a wide range of chemically

competent and electrocompetent cells. Moreover we provide Blue/white

screening for vectors containing inserts.

Quick-Stick Ligase Cat No. Size BIO-27027 50 Reactions BIO-27028 100 Reactions Features & Benefits • Fast - dramatically decreases time for DNA cloning • Catalyzes the joining of double-stranded DNA - for blunt or

cohesive ends • Compatible with downstream applications - no loss of

transformation efficiency Applications • Cloning of DNA from:

• PCR products • Plasmids • Cosmids • Genomic, phage and viral DNA

• Linker ligation • Re-ligation of linearized plasmids • Ligation of double-stranded oligonucleotides into vectors

(plasmid and phage) Description Quick-Stick Ligase is designed to carry out fast and efficient ligation of both cohesive and blunt-ended DNA at room

temperature. Quick-Stick Ligase is a T4 DNA Ligase that has been mutated to improve enzyme activity, and contains a specially developed 4x Quick-Stick Buffer. The enzyme catalyses the joining of two strands of DNA between the 5‟-phosphate and the 3‟-hydroxyl groups of adjacent nucleotides in either a blunt-ended or cohesive-ended configuration.

X-GAL Cat No. Size BIO-37035 1g Features & Benefits • Perfect for cloning - gives an intense blue precipitate upon

hydrolysis by β-galactosidase • Extremely pure - confirmed by HPLC

Applications • Blue/White cloning systems • Immunoblotting • Immunocytochemical assays • Microbiology and cell culture media

IPTG Cat No. Size BIO-37036 5g Features & Benefits • Induces E.coli lac operon activity - suitable for cloning and

protein expression procedures • Dioxane free - does not disrupt normal cell function • >99.6% purity - confirmed by HPLC Applications • Blue/white color screening • Induction of lac operon for protein expression • Genes controlled by the lac or tac promotor/operator

sequences are expressed to high levels in the presence of IPTG

Alpha-Select Gold Efficiency Cat No. Size BIO-85027 1ml (20 x 50µl)

Features & Benefits • Reduced recombination of cloned DNA - accommodates

larger plasmids • High efficiency - available in ≥109 cfu/μg, perfect for library

construction • Easy screening - with blue/white color selection • Convenient size - small aliquots reduce waste Applications • Transformation of large plasmids into bacterial cells • Generating cDNA libraries • Blue/white color screening Description α-Select Gold Competent Cells are directly comparable to DH5α cells. They contain a lacZ marker that provides α-complementation of the ß-galactosidase gene for blue/white color screening and are ideal for construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors. α-Select Gold Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids.

pUC19 DNA is also provided as a positive control.


Alpha-Select Silver Efficiency Cat No. Size BIO-85026 2ml (10 x 200µl) Features & Benefits • Reduced recombination of cloned DNA -designed to

accommodates larger plasmids • High efficiency -available in ≥108 cfu/μg, perfect for difficult

clones • Easy screening -with blue/white color selection • Convenient size -small aliquots reduce waste Applications • Transformation of large plasmids into bacterial cells • Generating clones • Blue/white color screening Description α-Select Silver Competent Cells are directly comparable to DH5α cells. They contain a lacZ marker that provides α-complementation of the ß-galactosidase gene for blue/white color screening and are ideal for difficult clone construction and blunt end ligation using plasmid-derived vectors. α-Select Silver Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids. pUC19 DNA is also provided as a positive control.

BIOBlue 10^9 Chemically Competent Cells Cat No. Size BIO-85037 1ml (20 x 50µl) Features & Benefits • Versitile -F‟ episome eliminates the need to select on minimal

media plates • High efficiency - available in ≥109 cfu/μg, perfect for phage

library construction • Easy screening - with blue/white color selection • Convenient size - small aliquots reduce waste Applications • Single-stranded plasmid rescue • Ideal strain for preparation of high-quality plasmid DNA • Routine cloning, using λ DNA or plasmid vectors • Blue/white color screening Description BIOBlue Chemically Competent Cells provide an ideal host for optimal preparation of both high-quality plasmid and Lambda phage vectors. The BIOBlue strain allows blue/white color screening through α-complementation of the ß-galactosidase gene. The endA1 phenotype allows production of high-quality plasmid DNA. Single-stranded DNA can be produced from plasmids containing a phage f1 origin. BIOBlue is also an excellent host for M13 and related filamentous phage and supports blue/white plaque screening and phage production. Maintenance of the F‟ episome in this strain is facilitated via selection with tetracycline, unlike strains such as JM101 which require growth on minimal media.

Alpha-Select Bronze Efficiency Cat No. Size BIO-85025 2ml (10 x 200µl) Features & Benefits • Reduced recombination of cloned DNA - designed to

accommodates larger plasmids • High efficiency - available in ≥107 cfu/μg, perfect for routine

subcloning • Easy screening - with blue/white color selection • Convenient size - small aliquots reduce waste Applications • Transformation of large plasmids into bacterial cells • Generating clones • Blue/white color screening Description α-Select Bronze Competent Cells are directly comparable to DH5α cells. They contain a lacZ marker that provides α-complementation of the ß-galactosidase gene for blue/white color screening and are ideal for subcloning using plasmid-

derived vectors. α-Select Bronze Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids. pUC19 DNA is also provided as a positive control.

CH3-Blue 10^8 Chemically Competent Cells Cat No. Size BIO-85039 1ml (10 x 100µl) Features & Benefits • Versatile -cloning of methylated and non-methylated DNA • High efficiency -available in ≥108 cfu/μg, perfect for difficult

clone construction • Easy screening -with blue/white color selection • Convenient size -small aliquotes reduce waste Applications • Cloning of methylated DNA • Ideal for subcloning • Generating cDNA libraries • Blue/white color screening Description CH3-Blue Chemically Competent Cells are a highly efficient derivative of E. coli K12, ideal for difficult clone construction using plasmid derived vectors. To facilitate the cloning of DNA that contains methylcytosine or 5-hydroxymethylcytosine, CH3-Blue lacks the E.coli restriction systems mcrA, mcrBC, mrr and hsdRMS. The addition of endA1 allows for cleaner preparations of DNA and better results in downstream applications. The elimination of non-specific digestion by Endonuclease I and the recA1 reduces the occurrence of non-specific recombination in cloned DNA.

This minimizes recombination events and improves the quality and yield of plasmid DNA. The lacZ mutation allows blue/white color screening and α-complementation of recombinants. Visit www.bioline.com for more informations

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CLONING

An astute application of ABM's enzyme platform, the cloning kits represent, a

strategic blend of ingredients that can simplify even the most challenging

cloning projects. These easy-to-use kits deliver faster reactions with high

sensitivity and increased yields thereby significantly reducing workload, time

input and overall project costs.

Ligation-Free Cloning Kit Cat No. Size E001 25 Reactions E002 100 Reactions Description The Ligation-Free Cloning kit is a versatile system to meet the demands of the routine and most challenging cloning projects. The progressive design has eliminated the requirement for compatible ends and unique restriction sites within the vector and inserts to be cloned. At the foundation of Ligation-Free Cloning technology is abm‟s proprietary Ligation-Free Enzyme Mix which reliably facilitates conjugation of up to 8 DNA inserts (PCR-generated sequences) and a linearized vector by recognizing a 15 bp overlap region at both ends. This 15 bp

overlap can be engineered by designing primers for amplification of your desired sequences to create inserts free from any redundant or unwanted base pairs. To eliminate the possibility of mutations associated with PCR amplification, scientists at abm have developed a DNA Polymerase with the highest proof-reading capability thus making this system the most reliable on the market. Applications • multiple fragment cloning and assembly. • cloning of any insert into any location of a chosen vector. • complete elimination of the dependence on availability of

restriction sites, phosphatase treatment and ligation. • inserts free from any redundant or unwanted base pairs. • save over 50% on reagent costs in comparison to other

products available on the market.

ProClone™ Competent Cells Cat No. Size E003 5ml (4x1.25ml) Description ProClone™ Competent Cells are high-efficiency, chemically competent DH5α (E. coli) cells that are optimized for use with abm‟s versatile range of expression vectors. Transformation efficiency greater than 1x10^6 cfu/µg can be achieved using abm‟s expression vectors, which are typically 3 to 4-fold larger in size and contain more complex genetic elements than the standard pUC19 plasmid. Reduced recombination and highly transformation efficiency make ProClone™ Competent Cells the premier choice for both routine and challenging subcloning projects. Applications • transformation efficiency: >1x10^6 cfu/µg with abm‟s

expression vectors (>10kb in size). • ideal for routine plasmid amplification. • reduces recombination and improves yield and quality of

plasmid DNA prepared from minipreps; can be sequenced directly.

• not Suitable for preparing unmethylated DNA.

Cloning Optimizer Cat No. Size E004 200 μl Description Cloning Optimizer consists of a proprietary enzyme that can completely eliminate background colonies when cloning. Treatment of PCR amplified DNA with this innovative optimizer will remove the trace amounts of plasmid template DNA that remain after PCR. Cloning Optimizer eliminates non-linearized template plasmids that cause false-positive colonies. Applications • Optimizes cloning efficiency • Reduces false-positive colonies when cloning

DNA End Repair Kit Cat No. Size G477 25 Reactions Description DNA End Repair Kit is designed to convert DNA fragmented by nebulisation, acoustic shearing, or nucleases into blunt ended DNA with 5´-phosphates and 3´-hydroxyl ends. This kit is provided as individual enzymes to meet the customer‟s needs and to provide maximum efficiency and flexibility in DNA sample preparation. The repaired DNA end product can subsequently be converted to DNA having 3´dA tails using abm‟s dA Tailing Kit (Cat. No. E009) or be used directly for blunt end cloning or blunt ended adaptor ligation. Applications • end repair of 1 - 5 μg of fragmented DNA. • RNA-Seq library construction. • downstream preparation for next generation sequencing. • blunt-end ligation into plasmid, cosmid, fosmid or BAC

vectors.

DNA dA-Tailing Kit Cat No. Size E009 25 Reactions Description The DNA dA-Tailing Kit efficiently adds a non-template dAMP (dA) to the 3‟ end of a blunt-ended DNA fragment. This incorporated 3‟-dA prevents concatemer formation and prepares the DNA fragment for subsequent ligation of adaptors or cloning vectors that have complementary 3‟-dT overhangs. The Klenow Fragment (3´→ 5´ Exo-) in this kit adds the 3‟-dA via its DNA polymerase activity. This enzyme lacks 5‟→3‟ exonuclease activity and has mutations that effectively abolish the inherent 3‟→5‟ exonuclease activity, thus preventing degradation of this 3‟-dA. This kit has been optimized to prepare compatible overhangs for the next step of DNA sample preparation for next generation sequencing. The Klenow Fragment (3´→ 5´ Exo-) enzyme is also available as a stand-alone enzyme (Cat. No. E038). Applications • DNA sample preparation • dA-Tailing of 1 - 5 μg of blunt ended DNA

Visit www.abmgood.com for more informations

Nucleic Acid Purification New molecular biology techniques require high quality DNA and RNA to be

carried out. Here we present the best solutions we can propone to get high yield DNA and RNA without the use of phenols. Increase your downstream results, with no harmful extraction kits.

DNA isolation kits for all types of samples Purified DNA is of the highest quality and integrity for sensitive downstream applications including PCR, qPCR, genotyping, sequencing and more Available in spin column format, 96 well plates, and magnetic beads

Genomic DNA Isolation Kit Cat No. Size 24700 50 preps 24750 100 preps For the isolation of genomic DNA from animal tissues, cells, bodily fluids, virus and swabs • Isolate genomic DNA from animal tissues, cells, bodily fluids,

viruses and swabs • Rapid and convenient spin column procedure • Purified DNA is of the highest quality and integrity for sensitive

downstream applications including PCR, qPCR, genotyping, sequencing and more

Cells and Tissue DNA Isolation Kit Cat No. Size 53100 50 preps For the rapid preparation of genomic DNA from cultured cells as well as various tissue samples. • Rapid spin column format • High yield • Excellent quality • DNA ready for any application including PCR, qPCR,

genotyping and more

Cells and Tissue DNA Isolation Micro Kit Cat No. Size 57300 50 preps • Optimized for small inputs of cells and tissues, such as Laser-

Captured Microdissection (LCM) • DNA is eluted in small elution volumes (20 – 40 μL) • Fast and easy processing using a rapid spin-column format • Isolate high quality genomic DNA

Cells and Tissue DNA Isolation Kit (Magnetic Bead

System) Cat No. Size 59100 50 preps 62500 2 x 96-well plates • Isolate genomic DNA from cultured cells as well as various

tissue types • Fast, reproducible and easy processing using a Magnetic

bead system • Isolate high quality genomic DNA • Recovered genomic DNA is compatible with various

downstream applications • Also available in a 96-well format that can be integrated

with a robotic automation system

Milk Bacterial DNA Isolation Kit Cat No. Size 21550 50 preps For the rapid purification of genomic DNA from various bacterial species found in milk

• Genomic DNA can be isolated from as few as 10 bacterial

cells in 1 mL of milk • Isolate genomic DNA from both Gram-negative and Gram-

positive bacteria in milk • Inhibitor-free DNA is ready for PCR, qPCR and more • Fast and efficient spin-column format

Fungi/Yeast Genomic DNA Isolation Kit Cat No. Size 27300 50 preps For the rapid purification of DNA from yeast cells and fungal spores or mycelium • Rapid spin column purification of genomic DNA from viable

yeast cells, fungal spores or mycelium, and bacteria including Gram-positive

• Bead tubes (provided) allow for effective mechanical homogenization

• Purified DNA is of high quality and integrity and compatible with any sensitive downstream applications such as PCR, qPCR, RFLP and more


DNA Purification Kits

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NUCLEIC ACID PURIFICATION - DNA

Bacterial Genomic DNA Isolation Kit Cat No. Size 17900 50 preps 17950 2 x 96-well plates For the isolation of genomic DNA from animal tissues, cells, bodily fluids, virus and swabs • Isolate genomic DNA from animal tissues, cells, bodily fluids,

viruses and swabs • Rapid and convenient spin column procedure • Purified DNA is of the highest quality and integrity for sensitive

downstream applications including PCR, qPCR, genotyping, sequencing and more

Phage DNA Isolation Kit Cat No. Size 46800 50 preps 46850 100 preps For the rapid purification of total DNA from bacteriophages • Isolate high quality DNA from a broad variety of phage

strains • High yields of total DNA • Fast and easy processing using a rapid spin-column format • No phenol or chloroform extractions or cesium chloride

banding required • High yields of DNA recovered 10-30 µg DNA from 106-1010

pfu/ mL of enriched phages

Plant/Fungi DNA Isolation Kit Cat No. Size 26200 50 preps 26250 250 preps 26900 2 x 96-well plates For rapid isolation of total DNA from plants and fungi • Rapid and simple procedure • Excellent quality and yield of DNA • Process a broad spectrum of plant species and filamentous

fungi • Isolate total DNA including pathogen DNA without phenol • Available in spin column format and 96-well format for high

throughput applications

Soil DNA Isolation Kit Cat No. Size 26500 50 preps For the rapid preparation of inhibitor-free DNA from all types of soil, including difficult samples with high humic acid content • Isolate total DNA from all microorganisms found in soil,

including bacteria, fungi and algae • Process all types of soil, including common soil samples such

as clay, loam and sandy soils and difficult samples with high humic acid such as compost, peat and manure

• Remove all humic acids using the provided Humic Acid Removal Columns and the OSR Solution

• Isolated DNA is free of inhibitors such as humic acid - ready for PCR applications and any metagenomic study

• Easy- to-use beads for extraction - fast without any time consuming sample grinding

Water RNA/DNA Purification Kit - 0.45 µm and 0.22 µm

Cat No. Size 26450 (0.45 µm) 50 preps 26400 (0.22 µm) 50 preps For convenient purification of RNA and DNA from microorganisms in water samples

• Isolate total DNA and RNA from all microorganisms found in

water, including bacteria, fungi and algae • RNA and DNA are both column purified simultaneously using

the same column • Elution contains concentrated DNA and RNA without the

need for further precipitation • Complete RNA (including microRNA) without phenol • Isolated RNA and DNA are of high quality and integrity for all

downstream applications • Available in 0.45 µm and 0.22 µm filter formats

FFPE DNA Purification Kit Cat No. Size 47400 50 preps

For the rapid and efficient extraction and purification of DNA from FFPE samples • Fast and easy processing using rapid and convenient spin-

columns • Isolate high quality and high yield DNA • DNA is free of inhibitors and ready for downstream use

including SNP (single nucleotide polymorphism) and short-tandem repeat (STR) genotyping

Urine Cell-Free Circulating DNA Purification Cat No. Size 56600 (Mini Kit) 50 preps 56700 (Mini Kit) 20 preps 56800 (Mini Kit) 10 preps • Isolate all sizes of circulating DNA from fresh, preserved or

frozen urine samples • Isolate viral DNA • Versatile urine input volumes:

250 µL - 2 mL for Mini Kits 2 mL - 10 mL for Midi Kits 10 mL - 30 mL for Maxi Kits

• Concentrate circulating DNA into a flexible elution volume ranging from (50 µL - 100 µL)

• Isolate inhibitor-free cell-free circulating DNA • Purify high-quality DNA in 15-20 minutes for Mini Kits, 40-45

minutes for Midi and Maxi Kits • Compatible with Norgen‟s Urine Preservative and other

commercially available urine preservatives

Urine DNA Isolation Kit for Exfoliated Cells or Bacteria Cat No. Size 47050 50 preps For the rapid and efficient purification of DNA from exfoliated cells or bacteria in urine • Isolate genomic DNA from either exfoliated cells or bacteria

found in urine • Isolate and detect genomic DNA from as little as 1 mL of

urine and up to 50 mL urine • High quality DNA for sensitive applications • Rapid processing time


Urine DNA Isolation Kit (Slurry Format) Cat No. Size 48800 50 preps 27100 1x96-well plate 50100 (Maxi Kit) 50 preps Fast, reliable isolation of urine DNA from:

3 mL to 25 mL samples 25 mL to 80 mL samples (Maxi Kit)

• Unlike other methods, Norgen‟s Urine DNA Isolation Kit (Slurry

Method) does not require any additional urine concentrating devices

• Fast processing time • Purify both genomic and apoptotic DNA with one protocol • Allows for purification of viral DNA from urine also • Available in Spin Column and 96-well format

Dried Blood Spot (DBS) DNA Isolation Kit Cat No. Size 36000 50 preps Rapid preparation of total DNA from dried blood spots • High quality DNA, free from RNA contamination • Isolate genomic DNA from anticoagulated and untreated

blood • Rapid and convenient spin column procedure

Blood DNA Isolation Kit Cat No. Size 46300 (Mini Kit) 50 preps 46380 (Mini Kit) 100 preps 51400 (Midi Kit) 20 preps 31200 (Maxi Kit) 12 preps For the rapid preparation of high quality DNA from whole blood • Input volumes:

Up to 200µL (Mini Kit) from 0.3 to 2 mL (Midi Kit) From 3 mL to 10 mL (Maxi Kit)

• High yield and high quality DNA ready for any application • Fast and convenient spin column protocol

Blood DNA Isolation 96-Well Kit Cat No. Size 46350 2 x 96-well For the rapid preparation of high quality DNA from whole blood • Input volumes up to 200 µL • High yield and quality DNA for any application • Fast and easy processing

Blood DNA Isolation Kit (Magnetic Bead System) Cat No. Size 59800 50 preps 62600 2 x 66-well plates • Isolate genomic DNA from cultured cells as well as various

tissue types • Fast, reproducible and easy processing using a magnetic

bead system • Recovered genomic DNA is compatible with various

downstream applications • Also available in a 96-well format that can be integrated

with a robotic automation system

Plasma/Serum Cell-Free Circulating DNA Purification Kit Cat No. Size 55500 (Micro Kit) 50 preps 55100 (Mini Kit) 50 preps 55600 (Midi Kit) 20 preps 55800 (Maxi Kit) 10 preps For rapid and simple purification of cell-free circulating DNA from plasma/serum samples • Isolate all sizes of circulating DNA from plasma and serum

samples • Isolate viral and bacterial DNA • Versatile plasma and serum input volumes:

10 µL - 200 µL for Micro Kit 200 µL - 500 µL for Mini Kit 1 mL – 4 mL for Midi Kit 5mL - 10 mL for Maxi Kit

• Concentrate circulating DNA into a flexible elution volume ranging from:

25 µL - 50 µL for Micro Kit 50 µL - 100 µL for Mini, Midi and Maxi Kits

• Isolate inhibitor-free cell-free circulating DNA • Purify high-quality DNA in 15-20 minutes for Micro and Mini

Kits, 40-45 minutes for Midi and Maxi Kits • Compatible with Streck Cell-Free DNA BCT® Tubes

Plasma/Serum Circulating DNA Purification Kit (Slurry Format) Cat No. Size 50600 (Mini Kit) 50 preps 51200 (Midi Kit) 20 preps 51300 (Maxi Kit) 10 preps For the rapid and simple isolation of circulating DNA from plasma/serum samples • Isolate all sizes of circulating DNA from plasma and serum

samples • Isolate viral and bacterial DNA • Versatile plasma and serum input volumes:

50 µL - 400 µL for Mini Kit 0.4 mL to 2 mL for Midi Kit 2mL to 10 mL for Maxi Kit

• Concentrate circulating DNA into small elution volumes • Different elution strategies depending on the downstream

application • Isolate inhibitor-free circulating DNA for any application

including PCR, qPCR, methylation-sensitive PCR • Compatible with Streck Cell-Free DNA BCT® Tubes

Stool DNA Isolation Kit Cat No. Size 27600 50 preps For the rapid and simple purification of bacterial and host DNA from stool and fecal samples • Simultaneous isolation of both host DNA and microbial DNA

(universal protocol) • Eliminates PCR inhibitors including humic acids • Fully compatible with Norgen's Stool Nucleic Acid Collection

and Transport Tubes • High quality DNA for sensitive downstream applications

including PCR, qPCR, Sequencing and microarray

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NUCLEIC ACID PURIFICATION - DNA

Food DNA Isolation Kit Cat No. Size 54500 50 preps For the isolation of DNA directly from food or from enriched microorganisms • Isolate DNA from a wide range of food materials. (e.g boiled,

fluid, processed or raw food products) • No hazardous chemicals required (e.g. phenol or

chloroform) • Effective lysis with Proteinase K and optional lysozyme

treatment • Fast (less than 15 minutes hands-on time) and convenient

processing using a rapid spin-column format • Wide compatibility with a variety of food products for GMO-

DNA isolation • Universal protocol for food related pathogen DNA isolation

(Gram positive and Gram negative)

Saliva DNA Isolation Kit Cat No. Size RU45400 50 preps For the rapid purification of high-quality DNA from preserved and fresh saliva samples • Sample collection is non-invasive and painless • Fast and easy processing using a rapid spin-column format • Isolate high quality genomic DNA • Compatible with preserved saliva samples collected using

Norgen‟s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples

Sputum DNA Isolation Kit Cat No. Size 46200 25 preps For the rapid purification of high quality DNA from sputum samples • Fast and easy processing using a spin column format • DNA can be isolated and detected from as little as 100 µL of

sputum • Includes Sputum Liquifier to reduce viscosity and facilitate

DNA isolation • Effective removal of PCR inhibitors • Fully compatible with Norgen‟s Sputum DNA Collection,

Preservation and Isolation Kit (Cat#RU46100).

Plasmid MiniPrep Kit Cat No. Size 13300 50 preps 46400 250 preps 25900 1 x 96-well plate 43300 4 x 96-well plates For rapid and convenient plasmid DNA preparations • High yield of plasmid DNA • Purify plasmids up to 13 kb • Plasmid DNA is ready for restriction digestion, bacterial

transformation, sequencing and more • Rapid spin-column format and 96-well format available

Microm DNA Isolation Kit Cat No. Size 55300 50 preps 55350 100 preps 55900 2 x 96-well plate • Isolate high quality total DNA from a variety of air filter types • Fast and easy processing using rapid spin-column format • Small elution volume of 20 µL • Isolate total DNA without compromising total yield • No phenol or chloroform extractions

Plasmid DNA MaxiPrep Kit Cat No. Size 46500 4 preps 46600 20 preps For the rapid preparation of high yields of plasmid DNA

Isolate up to 1.5 mg of plasmid DNA from 150 mL bacterial cultures Fast and efficient spin column procedure Purified DNA is of excellent yield and quality for restriction digestion, bacterial transformation, sequencing and more

Visit www.norgenbiotek.com for more informations

Bioline offers a range of kits for the isolation and purification of genomic and

plasmid DNA from a wide variety of samples, including tissue, cells, blood, serum

and plants, as well as purification of PCR products and from agarose gels.

The kits are designed to be rapid and easy-to-use, to give reproducibly high yield

and purity. Each kit is supplied with a comprehensive product manual containing

detailed protocols and additional helpful information, as well as a splash-proof

bench-top protocol for quick reference.

• Rapid extraction, with easy-to-use protocols

• Ultra-sensitive – perfect for low copy number samples

• High-yield, ideal where samples are limited

• High-purity DNA, ready for down-stream applications


ISOLATE II Plasmid Mini Kit Cat No. Size

BIO-52055 10 preps BIO-52056 50 preps BIO-52057 250 preps Features & Benefits • Rapid protocol - 25 minutes/18 preps • High-capacity - up to 40µg from 10ml of culture • High-purity plasmid DNA - typical A260/A280 ratio >1.8 • Universal - protocols for both high-copy and low-copy

plasmid Applications • Isolation of high-copy plasmid DNA • Isolation of low-copy plasmid DNA, P1 constructs and

cosmids • Isolation of plasmid DNA from Gram-positive bacteria • Plasmid DNA clean-up Isolated DNA is ready for downstream applications such as PCR, real-time PCR, cloning and sequencing.

ISOLATE II PCR and Gel Kit Cat No. Size BIO-52058 10 preps BIO-52059 50 preps BIO-52060 250 preps Features & Benefits • Quick protocol - for PCR clean-up (10 min) and DNA gel

extraction (20 min) • Excellent recovery rate - 70-95% • High purity DNA - typical A260/A280 ratio 1.8 - 1.9 • Highly purified DNA - ready for downstream applications Applications • Purification of PCR products • Isolation of DNA from TAE and TBE agarose gels • Purification of DNA from contaminants (enzymes, dNTPs, etc.) • Highly purified DNA is ready for applications such as cloning

and sequencing

ISOLATE II Blood DNA Kit Cat No. Size BIO-52062 10 preps BIO-52064 250 preps Features & Benefits • Fast - 30 min isolation protocol • High-quality - inhibitor-free DNA • High-purity DNA - typical A260/A280 ratio 1.6-1.9 • High-yield - from serum, plasma, buffy coat, platelets and

other body fluids Applications Isolation of genomic DNA from: • Human or animal blood, fresh or frozen • Blood treated with citrate, EDTA or heparin • Serum, plasma, buffy coat, platelets • Body fluids (e.g. amniotic fluid) Also suitable for isolation of bacterial and viral DNA from blood

ISOLATE II Genomic DNA Kit Cat No. Size

BIO-52065 10 preps BIO-52066 50 preps BIO-52067 250 preps Features & Benefits • Rapid protocol - ~20 minutes (after lysis) • High-purity genomic DNA - typical A260/A280 ratio 1.7 – 1.9 • High yield - up to 35µg genomic DNA • Broad spectrum kit - with over 17 protocols Applications Isolation of genomic DNA from: • Animal tissue and rodent tails • Paraffin embedded tissue • Clinical and forensic samples • Bacterial cells • Eukaryotic cells (yeast, cultured mammalian) Downstream applications such as multiplex PCR, Southern blotting and real-time PCR.

ISOLATE II Plant DNA Kit Cat No. Size BIO-52068 10 preps BIO-52069 50 preps BIO-52070 250 preps Features & Benefits • Fast - plant genomic DNA isolated in 30 minutes • High-purity DNA - typical A260/A280 ratio 1.6 - 1.9 • Choice of two optimized lysis buffers - for all sample types • Filters (shredders) included - for clarification of lysate Applications Isolation of genomic DNA from: • Fresh/frozen/lyophilized plant tissue • Dung, animal fecal, soil and compost samples • Herbarium specimens • Fungi Isolated DNA is ready for downstream applications such as PCR, real-time PCR and genotyping.

ISOLATE Fecal DNA Kit Cat No. Size BIO-52082 50 preps BIO-52038 100 preps Features & Benefits • Fast - 15-minute isolation protocol • High quality - inhibitor-free DNA • Straight forward - no need for organic denaturants or

proteinases • Universal - can be used with many different species Applications Isolation of fecal DNA from: • Human origin • Birds • Rodents (e.g. rats, mice) • Rabbits • Cattle Isolated DNA is ready for downstream applications such as PCR, real-time PCR, microarrays, sequencing and genotyping.

Visit www.bioline.com for more informations

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NUCLEIC ACID PURIFICATION - RNA

RNA Purification Kits

RNA isolation kits for all types of samples Extract high quality & purity total RNA including miRNA No phenol step required Bind & elute all RNA irrespective of size or GC content, without bias

Total RNA Purification Kit Cat No. Size 17200 50 preps 37500 100 preps For rapid purification of total RNA - including microRNA - without phenol • Extract high quality & purity total RNA including miRNA • No phenol step required • Bind & elute all RNA irrespective of size or GC content,

without bias

• Efficiently extract small RNA irrespective of GC content • Very sensitive & linear down to a few cells without the need

for carrier RNA • Convenient & fast spin column format • Isolate from a wide variety of specimens

Total RNA Purification Micro Kit Cat No. Size 35300 50 preps Rapid purification of total RNA -including microRNA - from small input amounts • Extract high quality & purity total RNA including miRNA • No phenol step required - isolate all RNA in one fraction • Elute extracted total RNA in small volume (20 µL) • Bind & elute all RNA irrespective of size or GC content,

without bias. • Efficiently extract small RNA irrespective of GC content • Very sensitive & linear down to a few cells without the need


Total RNA Purification Plus Kit Cat No. Size 48300 50 preps

48400 100 preps For the purification of total RNA - including microRNA - with rapid gDNA removal • Extract high quality & purity total RNA including miRNA • No phenol step required; isolate all RNA in one fraction • Genomic DNA Removal Column for efficient elimination of

gDNA • Bind & elute all RNA irrespective of size or GC content,

without bias • Efficiently extract small RNA irrespective of GC content • Very sensitive & linear down to a few cells without the need


Total RNA Purification Plus Micro Kit Cat No. Size 48500 50 preps Rapid purification of total RNA - including microRNA - from small input amounts • Extract high quality & purity total RNA including miRNA • Elute extracted total RNA in smaller volume (20 µL) • No phenol step required - isolate all RNA in one fraction • Genomic DNA Removal Column for efficient elimination of

gDNA

• Bind & elute all RNA irrespective of size or GC content, without bias.

• Efficiently extract small RNA irrespective of GC content • Very sensitive & linear down to a few cells without the need

for carrier RNA • Convenient & fast spin column format

Total RNA Purification Maxi Kit Cat No. Size

26800 8 preps • Extract high quality & purity total RNA including miRNA • No phenol step required - isolate all RNA in one fraction • Rapid processing in under 40 minutes • Isolate total RNA from a wide variety of specimens

Total RNA Purification 96-Well Kit Cat No. Size 24300 2 x 96-well plates For rapid high-throughput purification of total RNA including microRNA • Extract high quality & purity total RNA including miRNA • Convenient high throughput format for vacuum, centrifuge

or robot • Very consistent well-to-well RNA isolation • No phenol step required - isolate all RNA in one fraction

• Bind & elute all RNA irrespective of size or GC content, without bias

• Isolate from a wide variety of specimens

Animal Tissue RNA Purification Kit Cat No. Size 25700 50 preps For purification of total RNA (including microRNA) from all types of tissues including fiber-rich tissues • Extract high quality & purity total RNA including miRNA

• Isolate from a wide variety of animal tissues • No phenol step required - isolate all RNA in one fraction • Compatible with tissues stored in RNAlater® or Trizol® • Convenient & fast spin column format


microRNA Purification Kit Cat No. Size 21300 25 preps Rapid purification of microRNA without phenol; isolation and extraction of microRNA • Extract high quality & purity miRNA in 30 minutes • Two column kit- eliminate large RNA on first column and

capture microRNA on a second column • Elute microRNA in 25 µL ready for miRNA profiling • Process a wide range of samples- cell, tissue, bacteria, bodily

fluids, etc

Single Cell RNA Purification Kit Cat No. Size 51800 50 preps Rapid purification of total RNA -including microRNA - from small input amounts

• Fast and easy processing using rapid micro spin-column

format • Small elution volume of 8 µL ready for direct utilization in qRT-

PCR reactions • Isolate total RNA including microRNA in a concentrated

ready to use format • Isolate high quality total RNA from a variety of sources • RNA can be isolated from a single cell to 100,000 cells

Fatty Tissue RNA Purification Kit Cat No. Size 36200 25 preps For purification of total RNA (including microRNA) from animal tissues with high lipid content • Isolate high quality total RNA from hard to extract fatty

tissues • Isolate total RNA, including microRNA (miRNA) • No phenol or chloroform extractions • Fast and easy processing using rapid spin-column format • Genomic DNA removal without the use of nucleases

Plant/Fungi Total RNA Purification Kit Cat No. Size 25800 50 preps 31350 100 preps 25850 250 preps 31900 2 x 96-well plates For the rapid purification of total RNA (including microRNA) from plants and fungi • Extract total RNA, including virus & viroid RNA • Robust lysis buffer is well-suited to even challenging samples

such as pine needle, grape leaf, etc • Isolate total RNA (including microRNA) without phenol • Isolated RNA is of high quality, integrity and diversity • Also available in 96-well format for high throughput

applications

Plant microRNA Purification Kit Cat No. Size 54700 25 preps For the rapid purification of microRNA from plants • Fast and easy processing using a rapid spin-column format • No phenol or chloroform extractions • Isolate all small RNA molecules (<200 nt) • Minimal contamination from large RNA molecules and

genomic DNA • High quality small RNA can be used in various downstream

applications

Plant RNA/DNA Purification Kit Cat No. Size 24400 50 preps For simultaneous isolation of total RNA and DNA from the same plant sample • Robust Lysis Solution processes even the most challenging

plant species such as pine needle and grape • No phenol extractions • DNA and all sizes of RNA are recovered, including microRNA • High quality DNA and RNA are purified simultaneously using

the same spin column • No need to split the lysate

Soil Total RNA Purification Kit Cat No. Size 27750 50 preps For the rapid preparation of inhibitor-free total RNA from soil • Isolate high quality total RNA from a variety of soil samples • Process all types of soil, including common soil, compost and

manure • Remove all traces of humic acids and other inhibitors of PCR • Isolates all sizes of RNA, including microRNA, without phenol • Complete kit including bead tubes and humic acid removal

columns (HAR)

FFPE RNA Purification Kit Cat No. Size 25300 50 preps For the rapid and efficient extraction and purification of RNA (including microRNA) from FFPE samples • Extract total RNA (including microRNA) from FFPE samples • No phenol extraction step • Includes DNase for optional on-column DNA removal • Isolated RNA is of the highest quality and integrity • Isolate a diversity of RNA species

FFPE RNA Purification 96-Well Kit Cat No. Size 25400 2 x 96-well plates High throughput extraction and purification of RNA (including microRNA) from FFPE samples • Extract total RNA (including microRNA) from FFPE samples • No phenol extraction step • Includes DNase for optional on-plate DNA removal • Isolated RNA is of the highest quality and integrity • Process using either a vacuum manifold or centrifugation

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FFPE RNA/DNA Purification Plus Kit Cat No. Size 54300 50 preps Sequential isolation and purification of total RNA and genomic DNA from FFPE tissue samples • Fast and easy processing using rapid spin-column format • High yields and quality of nucleic acids • Separate fractionation of RNA and DNA • Isolate total RNA, from large rRNA down to microRNA

(miRNA) • No phenol or chloroform extractions

Plasma/Serum RNA Purification Kit Cat No. Size 55000 (Mini Kit) 50 preps 56100 (Midi Kit) 20 preps 56200 (Maxi Kit) 10 preps For rapid and simple purification of circulating RNA and Exosomal RNA from plasma/serum samples • Isolate all sizes of circulating and exosomal RNA, including

microRNA • Versatile plasma/serum input ranging from:

50 µL to 200 µL for Mini Kit 250 µL to 1.5 mL for Midi Kit 2 mL to 5 mL for Maxi Kit

• No phenol extractions • No carrier RNA • Bind and elute all RNA irrespective of size or GC content,

without bias • Concentrate circulating RNA and exosomal RNA into a

flexible elution volume ranging from: 10 µL to 25 µL for Mini Kit 50 µL to 100 µL for Midi and Maxi Kits

• Purify high-quality RNA in 15-20 minute for Mini Kit and 35-40 for Midi and Maxi Kits

Plasma/Serum RNA/DNA Purification Mini Kit Cat No. Size

55200 50 preps For rapid and simple sequential purification of circulating RNA, exosomal RNA and cell-free circulating DNA from plasma/serum samples • Isolate all sizes of circulating and exosomal RNA, including

microRNA • Isolate all sizes of circulating DNA from plasma and serum

samples • Isolate viral and bacterial DNA and RNA • Versatile plasma and serum input volumes (10 µL - 200 µL) • No phenol extractions • Bind and elute all RNA irrespective of size or GC content,

without bias • Concentrate circulating RNA, exosomal RNA and cell-free

circulating DNA into a flexible elution volume ranging from 10 µL to 25 µL

• Isolate inhibitor-free nucleic acids • Purify high-quality RNA and DNA in 30 minutes

Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) Cat No. Size 42800 50 preps 51000 (Mini Kit) 50 preps 29500 (Mini Kit) 1 X 96-well plate 50900 (Maxi Kit) 25 preps For rapid and simple isolation of circulating RNA including exosomal RNA from plasma/serum samples • Isolate all sizes of circulating and exosomal RNA, including

microRNA • Versatile plasma and serum input volumes :

0.25 mL to 5 mL standard kit 0.25 mL to 2 mL Mini Kit 2 mL to 5 mL Maxi Kit

• Concentrate circulating and exosomal RNA into small elution volumes

• Isolate inhibitor-free circulating RNA • Bind and elute all RNA irrespective of size or GC content,

without bias

Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Kit Cat No. Size 56300 (Mini Kit) 50 preps 56400 (Midi Kit) 20 preps 56500 (Maxi Kit) 10 preps

• Isolate all sizes of circulating DNA, circulating and exosomal RNA, including microRNA, viral DNA/RNA in one elution

• Versatile plasma/serum input ranging from: 50 µL to 200 µL for Mini Kit 250 µL to 1.5 mL for Midi Kit 2 mL to 5 mL for Maxi Kit

• No phenol extractions • No carrier RNA • Bind and elute all RNA irrespective of size or GC content,

without bias • Concentrate circulating DNA, circulating and exosomal

RNA, viral DNA and viral RNA into a flexible elution volume ranging from 10 µL to 25 µL for mini Kit and 50 µL to 100 µL for Midi and Maxi Kits

• Purify high-quality RNA/DNA in 15 - 20 minutes for Mini Kit and 35-40 minutes for Midi and Maxi Kits

• Compatible with Streck Cell-Free DNA BCT® Tubes

Plasma/Serum Circulating Nucleic Acid Purification Kit (Slurry Format) Cat No. Size 53300 (Mini Kit) 50 preps 53400 (Maxi Kit) 25 preps For rapid and simple isolation of circulating nucleic acids from plasma/serum samples

• Isolate all sizes of circulating and exosomal RNA, including

microRNA • Isolate all sizes of circulating DNA from plasma and serum

samples • Versatile plasma and serum input volumes:

0.25 mL to 2 mL for Mini Kit

2 mL to 5 mL for Maxi Kit • Concentrate circulating nucleic acids into small elution

volumes • Isolate inhibitor-free circulating nucleic acids • Bind and elute all RNA irrespective of size or GC content,

without bias


Leukocyte RNA Purification Kit Cat No. Size 21200 50 preps 37800 2 x 96well plates For the rapid extraction and purification of total RNA from leukocytes • Fractionate leukocytes from whole blood in minutes with

provided RBC Lysis Buffer • Isolate total RNA, including microRNA, without phenol • Rapid and convenient spin-column format • Purified RNA is ready for any downstream application

including RT-PCR, qRT-PCR, NGS, arrays and more • Rapid and convenient 96-well plate format for high

throughput applications

Preserved Blood RNA Purification Kit I (for use with

Tempus Blood RNA Tubes) Cat No. Size 43400 50 preps Rapid isolation and purification of total RNA from blood that has been preserved using Tempus™ Blood RNA Tubes • Compatible with Tempus™ Blood RNA Tubes • Isolate true total RNA including siRNA and microRNA • No phenol extractions • Purify high-quality RNA in 30 minutes

Preserved Blood RNA Purification Kit II (for use with

PAXgene Blood RNA Tubes) Cat No. Size 43500 50 preps Rapid isolation and purification of total RNA from blood that has been preserved using PAXgene Blood RNA Tubes • Compatible with PAXgene Blood RNA Tubes • Isolate true total RNA including siRNA and microRNA • No phenol extractions • Purify high-quality RNA in 30 minutes

Cytoplasmic and Nuclear RNA Purification Kit Cat No. Size 21000 50 preps 37400 100 preps For the convenient purification of cytoplasmic and nuclear RNA from cultured cells and tissues • Excellent separation and purification of cytoplasmic and

nuclear RNA • Convenient and fast spin column format • High quality and yield of RNA • Isolate full diversity of RNA (including microRNA) without

phenol • Purified RNA is ready for any application including RT-PCR,

qRT-PCR, RNA-Seq, arrays and more • Cytoplasmic RNA is free of DNA and ready for direct use in

RT-PCR/qRT-PCR

Stool Total RNA Purification Kit Cat No. Size 49500 50 preps A convenient and rapid method to purify total RNA from small amounts of stool samples

• Simultaneous isolation of both host RNA and microbial RNA

(universal protocol) • Isolate full diversity of RNA from large RNA down to small and

microRNAs • Eliminates PCR inhibitors including humic acids • High quality RNA for sensitive downstream applications

Stool Nucleic Acid Isolation Kit Cat No. Size 45600 50 preps A convenient and rapid method to isolate total DNA and RNA from fresh, frozen and preserved stool samples • Simultaneous isolation of both host and microbial DNA and

RNA • Eliminates PCR inhibitors including humic acids • High quality total RNA and DNA for sensitive downstream

applications

Urine Cell-Free Circulating RNA Purification Kit Cat No. Size 56900 (Mini Kit) 50 preps 57000 (Midi Kit) 20 preps 57100 (Maxi Prep) 10 preps • Isolate all sizes of circulating and exosomal RNA, including

microRNA • Versatile urine input ranging from:

250 µL to 2 mL for Mini Kit 2 mL to 10 mL for Midi Kit 10 mL to 30 mL for Maxi Kit

• No phenol extractions • No carrier RNA

• Bind and elute all RNA irrespective of size or GC content, without bias

• Concentrate circulating RNA and exosomal RNA into a flexible elution volume ranging from 50 µL to 100 µL

• Purify high-quality RNA in 25 - 30 minutes for Mini Kit and 40-45 minutes for Midi and Maxi Kits

Urine Cell-Free Circulating and Viral Nucleic Acid Purification Kit Cat No. Size 59900(Mini Kit) 50 preps 60000(Midi Kit) 20 preps 60100(Maxi Prep) 10 preps • Isolate all sizes of circulating DNA, circulating and exosomal

RNA, including microRNA, viral DNA/RNA in one elution • Versatile urine input ranging from:

250 µL to 2 mL for Mini Kit 2 mL to 10 mL for Midi Kit 10 mL to 30 mL for Maxi Kit

• No phenol extractions • No carrier RNA • Concentrate circulating RNA and exosomal RNA into a

flexible elution volume ranging from 50 µL to 100 µL • Purify high-quality RNA in 25 - 30 minutes for Mini Kit and 40-45

minutes for Midi and Maxi Kits • Compatible with fresh, frozen or preserved urine sample

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Urine Exfoliated Cell RNA Purification Kit Cat No. Size 22550 25 preps For the rapid purification of total RNA (including microRNA) from exfoliated cells in urine • Purify all sizes of RNA (including microRNA) without the need

for phenol • Isolate and detect total RNA from 1 mL and up to 50 mL

urine • Provides high quality RNA for sensitive applications - isolate

RNA from as little as 100 cells • Rapid processing time from sampling to downstream testing

Urine microRNA Purification Kit Cat No. Size 29000 25 preps Rapid purification of microRNA from urine without phenol • Isolate high quality total RNA and all sizes of circulating and

exosomal RNA, including microRNA • Small urine input ranging from as low as 0.5 mL to 1 mL • No phenol extractions • Very sensitive and linear down to a few cells without the

need for carrier RNA • Bind and elute all RNA irrespective of size or GC content,

without bias • Rapid and convenient spin-column protocol

Urine Total RNA Purification Maxi Kit (Slurry Format) Cat No. Size 29600 50 preps 29650 1 x 96-well plate Rapid purification of total RNA from 5 mL - 10 mL urine without phenol • Isolate high quality total RNA and all sizes of circulating and

exosomal RNA, including microRNA • No phenol extractions • Very sensitive and linear without the need for carrier RNA

• Bind and elute all RNA irrespective of size or GC content, without bias

• Isolate inhibitor-free urinary RNA • Slurry/Spin column and Slurry/96-well format available

Urine Exosome RNA Isolation Kit Cat No. Size 47200 50 preps A rapid procedure for the isolation of exosomal RNA from urine sample • Isolation of exosomal RNA molecules from urine samples • Rapid and convenient spin-column protocol • Isolate inhibitor-free urinary microRNA for any application • Purification of exosomal proteins for western blot analysis • Purified exosomes can be analyzed using NanoSight® or

Electron Microscopy for assessing the approximate exosome size range and concentration

RNA/DNA/Protein Purification Plus Kit Cat No. Size 47700 50 preps For sequential isolation of total RNA, genomic DNA and total proteins from the same sample • Sequentially purify total RNA (and miRNA), DNA and proteins

from a single sample • No sample splitting or need to use phenol or precipitation

methods • Purify RNA/DNA/Protein from cultured animal cells, tissues,

blood, bacteria, yeast, fungi or plants • Rapid and efficient spin column procedure - all done in 30

minutes • Proteins are purified on column and are soluble in the elution

buffer. No further cleaning is required

RNA/DNA/Protein Purification Plus Micro Kit Cat No. Size 51600 50 preps For sequential isolation of total RNA, genomic DNA and total proteins from the same sample • Sequentially purify RNA (and miRNA), DNA and proteins from

a single sample • Small elution volume down to 20 µL with a specialized

column • No sample splitting, no need to use phenol or precipitation

methods • Proteins are purified on column and are soluble in the elution

buffer • Proteins require no further cleaning - ready for Western blot

and Mass spectrometry • Suitable for cells, tissues, stem cells, CTC, small input of

samples • Rapid and efficient spin column procedure - all done in 30

minutes

RNA/DNA Purification Kit Cat No. Size 48700 50 preps For sequential isolation of total RNA and genomic DNA from the same sample • Sequentially isolate and purify total RNA and DNA from a

single sample • Two column system: one for DNA and one for RNA • The RNA column is for the purification of total RNA including

microRNA • No need to split the lysate, or to use phenol or precipitation

methods • Rapid and efficient spin column procedure - it takes only 30

minute

Visit www.norgenbiotek.com for more informations


Bioline ISOLATE II RNA Kits are designed for the isolation and purification of RNA

from a wide variety of samples, including samples that are sometimes difficult to

process. The kits are supplied with filters (shredders) and DNase where required, to

enhance sample homogenization and remove genomic DNA. Each kit is supplied

with a comprehensive product manual containing detailed protocols and

additional helpful information, as well as a splash-proof bench-top protocol for

quick reference.

• Latest silica technology for outstanding yield, purity and integrity (RIN)

• Phenol-free for rapid and unbiased isolation

• Ideal for highly sensitive applications

ISOLATE II Biofluids RNA Kit Cat No. Size BIO-52086 50 preps Features & Benefits • High quality total RNA - efficient recovery from challenging

biofluids and viral samples • High purity and yield - ideal for samples with low RNA

content • Complete RNA diversity - all sizes of RNA isolated, from large

mRNA, viral RNA down to miRNA and siRNA • Rapid, phenol-free protocol (20 min/10 preps) - increase your

throughput • DNase I included - efficient digestion of genomic DNA for

sensitive applications Applications Isolation of all sizes of RNA from: • Biofluids including blood, serum, plasma, urine, saliva, CSF • Viruses Pure, isolated RNA ready for all downstream applications such as real-time RT-PCR, RNA-seq and microarrays.

ISOLATE II FFPE RNA/DNA Kit Cat No. Size BIO-52087 50 preps Features & Benefits • Reliable - RNA/DNA from a single FFPE sample with no

splitting of the lysate, reducing variability and inconsistent results

• Isolate diverse RNA and DNA species - all sizes of RNA from large mRNA down to miRNA and all DNA above 80bp

• Reversal of formalin crosslinks - optimized for high nucleic acid yield and purity

• Phenol-free - all RNA including small RNA purified without bias

• DNase I included - complete removal of genomic DNA from RNA fraction

Applications The purified RNA can be used in a number of downstream applications including: • Real-Time RT-PCR • RNA-seq • Expression microarray The purified genomic DNA can be used in: • Real-Time PCR • Multiplex PCR • Methylation analyses by bisulfite conversion and sequencing • Next generation sequencing • SNP microarray

ISOLATE II miRNA Kit Cat No. Size BIO-52083 25 preps Features & Benefits • Rapid, phenol-free workflow - unbiased small RNA isolation • Novel separation matrix - highest yields and purity • Highly specific small RNA enrichment - highest recovery of

miRNA, siRNA and all other small RNA <200nt • Parallel large RNA purification (optional) - high quality large

RNA >200nt • DNase I included - highly efficient digestion of genomic DNA Applications Isolation of miRNA and other small RNA from • Mammalian tissue and cell lines • Bacteria and yeast • Biofluids (saliva, semen, urine, blood, plasma, serum) Fully compatible with highly sensitive applications such as real-time RT-PCR, microarrays, single-cell assays and next-generation RNA sequencing (small RNA-seq).

ISOLATE II Plant miRNA Kit Cat No. Size BIO-52084 25 preps Features & Benefits • Rapid, phenol-free protocol - unbiased small RNA isolation • Novel separation matrix - highest yields of pure miRNA and

small RNA • Highly specific small RNA enrichment - capture all small RNA

species (<200nt) from plant cells and tissues

• Parallel high quality large RNA (>200nt) purification from same sample

• BioFilter and DNase I included - enhanced sample homogenization and efficient elimination of genomic DNA

Applications Isolation of RNA from: • Fresh plant cells and tissue • Frozen and lyophilized plant tissue Parallel isolation of high quality large RNA >200nt from the same sample types. Fully compatible with miRNA real-time RT-PCR assays, microarrays, single-cell assays and next-generation RNA sequencing (small RNA-seq).

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ISOLATE II RNA/DNA/Protein Kit Cat No. Size BIO-52085 50 preps Features & Benefits • Rapid, phenol-free workflow - reliably purify

RNA/DNA/Proteins from a single sample in ≤30 mins • Novel separation matrix - superior yields of high quality

molecules • Complete diversity of RNA - all sizes of RNA recovered from

mRNA to miRNA/siRNA • Purify small samples - sequential isolation ideal for precious

or small biopsy material • DNase I included - f or complete removal of genomic DNA in

RNA purification Applications Isolation of total RNA (including small RNA)/DNA/Protein from: • Mammalian tissue • Eukaryotic cell lines • Bacteria, yeast, fungi • Blood and other biofluids Pure, isolated molecules ready for all downstream applications such as real-time RT-PCR, RT-PCR, RNA-seq, genotyping, 2D gel electrophoresis, Western blotting, SDS-PAGE and Mass Spectrometry.

ISOLATE II RNA Mini Kit Cat No. Size BIO-52071 10 preps BIO-52072 50 preps BIO-52073 250 preps Features & Benefits • Rapid protocol - 30 min/6 preps • High purity RNA - typical A260/A280 ratio 1.9 - 2.1 • High quality - RNA integrity number (RIN) >9 • Filters (shredders) included - to enhance sample

homogenization • DNase I included - for complete removal of genomic DNA Applications Isolation of RNA from: • Mammalian tissues • Eukaryotic cells • Bacterial cells • Yeast • Biological fluids (cell-free) Isolated RNA is ready for downstream applications such as reverse transcription and one-step real-time PCR.

ISOLATE II RNA Micro Kit Cat No. Size BIO-52074 10 preps BIO-52075 50 preps Features & Benefits • Rapid protocol - 40 min/12 preps • Perfect for small samples - biopsy or single cells • High yield and purity - typical A260/A280 ratio 1.9 - 2.1 • Extra filters (shredders) and DNase I included - for excellent

RNA recovery Applications • Isolation of RNA from small amounts of samples: • Single cells • Pellets of cultured cells • Cryosections • Laser-captured or flow sorted cells • Small amounts of tissues e.g. biopsy materials Isolated RNA is ready for downstream applications such as reverse transcription and real-time PCR.

ISOLATE II RNA Plant Kit Cat No. Size BIO-52076 10 preps BIO-52077 50 preps

Features & Benefits • Rapid protocol - 30 minutes after homogenization • High purity RNA - ready for downstream applications • Extra filters (shredders) included - to enhance sample

homogenization • Two optimized extraction buffers - for all plant samples Applications Isolation of RNA from: • Fresh plant cells and tissue • Frozen and lyophilized plant tissue • Filamentous fungi Isolated RNA is ready for downstream applications such as real-time PCR and reverse transcription/cDNA synthesis.



End-Point PCR Together with our partners, we really care about your PCR experiments. Trust us

and try our high-quality polymerases and kits. We have a full range of polymerases to meet all your experimental needs (cloning, genotyping, routine PCR, GC rich samples, etc). Ask us at [email protected] which is the most suitable Taq for you!

For over 20 years Bioline has been engaged in the development and

manufacture of one of the broadest portfolios of premium quality PCR enzymes for

use in a wide range of applications. Our commitment to PCR-based research is

demonstrated with the introduction of MyFi™ RANGER and the MyTaq™ product

range, a new generation of highest performance DNA polymerases.

Our PCR Enzyme Guide will help you choose the polymerase best suited to your

research requirements. Each DNA polymerase possesses different characteristics

so, to achieve optimal research results, it is important to choose the right

polymerase for your application.

Choosing the Right DNA Polymerase

Successful PCR depends pon thwo crucial components, an optimized reaction buffer and a high-quality,

thermostable DNA polymerase (such as Taq DNA polymerase).

Four basic properties of DNA polymerases define the best enzyme for your particular research needs:

Thermal stability, extension rate, fidelity (the ability to replace incorrectly incorporated nucleotides) and

processivity (the probability a polymerase will detach from DNA during extension).

Different configurations of these four variables have produced different classes of DNA polymerase, namely:

• Standard DNA polymerases

Suitable for routine PCR, such as detection of amplified product and estimation of product size,

producing a single-based „A‟ overhang, enabling direct insertion into T/A cloning vectors.

• Hot-start (HS) polymerases

Used to suppress nonspecificproduct amplification during setup and increase yield of desired product.

Hot-start is useful when DNA template amounts are low, DNA templates are highly complex or several

pairs of primers are used, as in multiplex PCR.

• High-fidelity polymerases

These remove erroneous bases incorporated in the growing DNA strand, increasing the accuracy of

DNA synthesis from template DNA. For cloning and expression of amplified product, mutagenesis

studies and related applications, proofreading enzymes should be used.

• Polymerases for amplification of long amplicons

Amplification of long amplicons combines the processivity of standard DNA polymerases with the

accuracy of a proofreading polymerase. This is achieved by blending two polymerases with an

optimized buffer, to give amplicons as long as 25kb from genomic DNA.

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END-POINT PCR

Properties and Applications

Best Choice

Recommended

Suitable

You should choose your PCR Enzyme as

follows:

• For general and routine PCR, use a

standard DNA polymerase (myTaq)

• For clean product and high yield, use a hot-

start polymerase (MyTaq HS, MyFi)

• For gene expression or mutagenesis

experiments, use a proofreading enzyme

(ACCUZIME, VELOCITY)

• For long amplicons, use a long-range DNA

polymerase (RANGER)

Applications

My

taq

TM H

S

My

taq

TM

My

FiT

M

RA

NG

ER

VELO

CIT

Y

AC

CU

ZIM

ETM

Standard PCR

Long PCR

Fast PCR

Multiplex PCR

GC-Rich PCR

Genotyping

Low-Copy PCR

Universal Polymerases

Properties

Hot-Start

High-Fidelity

High Specificity PCR

Pre Mix

Direct Loading


MyTaq™ DNA Polymerase Cat No. Size BIO-21105 500 Units BIO-21106 2500 Units BIO-21107 5000 Units Features & Benefits • New generation polymerase - for superior performance • Novel buffer system - optimized and including dNTPs and

MgCl2 • Robust - high-yield across a wide range of templates • Available with red dye - for direct gel loading • Convenient - all-in-one mastermix option available Applications • Genotyping • Colony PCR • High-throughput PCR • Fast PCR reactions • GC-rich amplification

MyTaq™ Red DNA Polymerase Cat No. Size BIO-21108 500 Units BIO-21109 2500 Units BIO-21110 5000 Units Features & Benefits • New generation polymerase - for superior performance • Novel buffer system - optimized and including dNTPs and

MgCl2 • Robust - high-yield across a wide range of templates • Red dye - for direct gel loading • Convenient - all-in-one mastermix option available Applications • Genotyping • Colony PCR

• High-throughput PCR • Fast PCR reactions • GC-rich amplification

MyTaq™ Mix Cat No. Size BIO-25041 200 x 50µl Reactions BIO-25042 1000 x 50µl Reactions Features & Benefits • Convenient - all-in-one mastermix • Novel buffer system - optimized and including dNTPs and

MgCl2 • Robust - high-yield across a wide range of templates • Available with red dye - for direct gel loading Applications • Genotyping • Colony PCR • High-throughput PCR • Fast PCR reactions

• GC-rich amplification

MyTaq™ Red Mix Cat No. Size BIO-25043 200 x 50µl Reactions BIO-25044 1000 x 50µl Reactions Features & Benefits • New generation polymerase- for superior performance • Novel buffer system - optimized and including dNTPs and

MgCl2 • Robust - high-yield across a wide range of templates • Red dye - for direct gel loading • Convenient - all-in-one mastermix Applications • High-throughput PCR • Specific amplification of complex templates • Robust amplification of GC-rich sequences • Colony PCR • Routine PCR applications • TA cloning

MyFi™ DNA Polymerase Cat No. Size BIO-21117 250 Units BIO-21118 500 Units BIO-21119 2500 Units Features & Benefits • New generation DNA polymerase complex - provides robust

PCR with 3.5-fold higher fidelity than wild-type Taq polymerase

• Antibody-based hot-start polymerase - enhances specificity and allows room-temperature reaction set-up

• High processivity - efficiently amplifies complex mammalian DNA fragments up to 10kb

• Novel 5x buffer system - ensures reliability, reproducibility and convenience

Applications • Routine PCR applications • Specifically developed for TA cloning • High throughput • Good for low copy PCR assays • PCR requiring high specificity combined with higher fidelity

MyFi™ Mix Cat No. Size BIO-25049 100 x 50µl Reactions BIO-25050 500 x 50µl Reactions Features & Benefits • Convenient - ready-to-use, all-in-one 2x mastermix • New generation DNA polymerase complex - provides robust

PCR with 3.5-fold higher fidelity than wild-type Taq polymerase

• Antibody-based hot-start polymerase - enhances specificity and allows room-temperature reaction set-up

• High processivity - efficiently amplifies complex mammalian DNA fragments up to 10kb

Applications • TA Cloning • Long PCR • Low-copy PCR assays • PCR of difficult DNA templates (problematic GC/AT-rich

genomic DNA) • High-fidelity PCR assays (library amplification)

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END-POINT PCR

MyTaq™ HS DNA Polymerase Cat No. Size BIO-21111 250 Units BIO-21112 1000 Units BIO-21113 2500 Units Features & Benefits • Universal DNA polymerase - recommended for most

applications • Fast - complete PCR reaction in less than 30 minutes • Antibody-based hot-start polymerase - for highest specificity • Superior performance - highly optimized buffer system,

including ultra-pure dNTPs and MgCl2 Applications • Fast PCR reactions • Colony PCR • Genotyping • Multiplexing • Low-copy PCR assays • Specific amplification of difficult templates (GC-rich) • Assays with prolonged reaction set-up

MyTaq™ HS Mix Cat No. Size BIO-25045 200 x 50µl Reactions BIO-25046 1000 x 50µl Reactions Features & Benefits • All-in-one mastermix formulations - for enhance

reproducibility • Fast - complete PCR reaction in less than 30 minutes • Antibody-based hot-start polymerase - for highest specificity • Superior performance - highly optimized buffer system,

including ultra-pure dNTPs and MgCl2 Applications • Fast PCR reactions • Colony PCR • Genotyping • Multiplexing • Low-copy PCR assays • Specific amplification of difficult templates (GC-rich) • Assays with prolonged reaction set-up

MyTaq™ HS Red DNA Polymerase Cat No. Size BIO-25045 1000 Units BIO-25046 2500 Units Features & Benefits • Universal DNA polymerase - recommended for most

applications • Fast - complete PCR reaction in less than 30 minutes • Antibody-based hot-start polymerase - for highest specificity • Superior performance - highly optimized buffer system,

including ultra-pure dNTPs and MgCl2 • Red dye - for direct gel loading • All-in-one mastermix available- for enhance reproducibility Applications • Fast PCR reactions • Colony PCR • Genotyping • Multiplexing • Low-copy PCR assays • Specific amplification of difficult templates (GC-rich) • Assays with prolonged reaction set-up

MyTaq™ HS Red Mix Cat No. Size BIO-25047 200 x 50µl Reactions BIO-25048 1000 x 50µl Reactions Features & Benefits • All-in-one mastermix formulations - for enhance

reproducibility • Fast - complete PCR reaction in less than 30 minutes • Antibody-based hot-start polymerase - for highest specificity • Superior performance - highly optimized buffer system,

including ultra-pure dNTPs and MgCl2 • Red dye - for direct gel loading

Applications • Fast PCR reactions • Colony PCR • Genotyping • Multiplexing • Low-copy PCR assays • Specific amplification of difficult templates (GC-rich) • Assays with prolonged reaction set-up

RANGER DNA Polymerase Cat No. Size BIO-21121 250 Units BIO-21122 500 Units Features & Benefits • Fast antibody-based hot-start polymerase - for highest

specificity and speed • Superior performance - unique buffer system, including ultra-

pure dNTPs and MgCl2 • Higher-fidelity than Taq - Suitable for all long range

applications Applications • Validated for human genomic DNA up to 25 kb • Suitable for TA cloning

RANGER Mix Cat No. Size BIO-25052 500 x 50µl Reactions Features & Benefits • Convenient - all-in-one mastermix for ease of set-up • Fast antibody-based hot-start polymerase - for highest

specificity and speed • Superior performance - unique buffer system, including ultra-

pure dNTPs and MgCl2 • Higher-fidelity than Taq - Suitable for all long range

applications Applications • Validated for human genomic DNA up to 25kb • Suitable for TA cloning


VELOCITY DNA Polymerase Cat No. Size BIO-21098 250 Units BIO-21099 500 Units Features & Benefits • High-fidelity DNA polymerase - minimizing errors • High-processivity - for longer amplicons • Fast amplification - for short run times • Ideal for a wide range of templates - including long

templates greater than 5kb Applications • Cloning techniques where high fidelity is desirable • GC-rich templates • Blunt-end cloning • Site directed mutagenesis

ACCUZYME™ DNA Polymerase Cat No. Size BIO-21051 250 Reactions BIO-21052 500 Units Features & Benefits • High-fidelity - ideal for subsequent cloning • High processivity - amplifies fragments up to 5 kb Applications • Cloning techniques where high fidelity is desirable • GC-rich templates • Blunt-end cloning • Site directed mutagenesis

ACCUZYME™ Mix Cat No. Size BIO-25028 500 x 50µl Reactions Features & Benefits • High-fidelity - ideal for subsequent cloning • High processivity - amplifies fragments up to 5 kb • Pre-optimized mastermix - for enhance reproducibility Applications • Cloning techniques where high fidelity is desirable • GC-rich templates • Blunt-end cloning • Site directed mutagenesis

MyTaq™ Blood-PCR Kit Cat No. Size

BIO-25054 250 Reactions Features & Benefits • Extraction-free – eliminate complex DNA extraction protocols • Novel buffer system - specially formulated to overcome

blood inhibition for highest PCR success and sensitivity • Versatile – perfect for a wide range of human and animal

bloods including EDTA, citrate and heparin preserved samples

• Powered by MyTaq HS DNA Polymerase - for highest specificity and yield

• Application validated – ideal for multiplexing, GC rich templates and longer amplicons

Applications • SNP genotyping • Human and animal blood DNA extraction and amplification • Blood preserved with heparin, citrate, or EDTA

MyTaq™ Extract-PCR Kit Cat No. Size BIO-21126 100 Reactions

BIO-21127 500 Reactions Features & Benefits • Rapid extraction protocol - high-yield, PCR-ready DNA in 15

minutes • Easy-to-use - eliminates complicated DNA extraction

procedures • Convenient format - single-tube extraction minimizes

contamination risks • Proven performance - powered by MyTaq™ HS for fast and

highly-specific amplification Applications • Ideal for high-throughput genotyping from mammalian

tissues • Detection of transgenes • Knockout analysis

MyTaq™ Plant-PCR Kit Cat No. Size BIO-25055 250 Reactions BIO-25056 500 Reactions Features & Benefits • Fast - eliminate complex, slow and costly DNA extraction

steps, thereby reducing time to results • Robust- specially developed to overcome common plant-

derived PCR inhibitors such as polyphenolics and polysaccharides for highest PCR success rates and improved

sensitivity • Versatile - perfect for a wide range of plant species,

avoiding the need for further optimization, thereby minimizing setup time and reducing cost

• Streamlined workflows - ideal for fast genotyping in plant genetic studies, mutation detection, confirming transgenic plant and knockout analysis

Applications • Genotyping for plant genetic study • Mutation detection • Transgenic detection • Knockout analysis

MyTaq™ One-Step RT-PCR Kit Cat No. Size BIO-65048 250Reactions BIO-65049 100 Reactions Features & Benefits • Extremely sensitive - blend of RT and novel MyTaq HS • Highly optimized - for detection of low-copy genes • Superior performance - overcomes secondary structure in

difficult and GC-rich targets • High-quality - full-length cDNA from as little as 3pg total RNA • Simple to use - all-in-one mix for highly-specific and

ultrasensitive products for use in downstream applications Applications • Gene-expression analysis • Transcription analysis • Gene cloning • Multiplex RT-PCR


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REAL TIME PCR

Real Time PCR Tired of boring time-comsuming qPCR runs? GoFASTER!! Try our brand-new

mastermixes and see the difference. Get the higher sensitivity and specificity on the market.. but always at a reasonable price! No matter if you're used to probe or SYBR assays, no matter the instrument you use, we have the right mastermix for you.

SensiFAST™ uses the latest developments in real-time PCR to realize the fastest

cycling times and greatest sensitivity, without compromising accuracy,

reproducibility or performance. SensiFAST™ kits can be used on all real-time

instruments and are also perfect for the new generation of fast-cyclers.

Choosing the Right Mastermix

One-step real-time RT-PCR is now the gold standard technique for mRNA detection and quantification.

Designed for reverse transcription and real-time PCR amplification in a single tube, it is sesitive enough to

enable quantification of RNA from a single cell. SensiFAST™ One-Step Kits are ideal for situations where the

same genes must be amplified repetitively, such as in high-throughput detection of specific target using well-

established reaction conditions. However, gene-specific primers are required and all of the cDNA is consumed

in the real-time PCR step.

In two-step real-time RT-PCR, reverse transcription and PCR are performed as two separate reactions, allowing

the cDNA and real-time PCR reactions to be optimized separately, resulting in higher yields of cDNA during the

RT step than for the one-step procedures, making it more sensitive than one-step real-time RT-PCR. RNA is

converted to cDNA in the first step, using a choice of random hexamers, oligod(T)n primers, or gene-specific

primers, or for unbiased reverse transcription a mixture of random hexamers and anchored oligo d(T)n (such as

in the SensiFAST™ cDNA Synthesis Kit). Either a portion of the RT reaction is diluted into the real-time PCR in the

second step, or the RT reaction can be extracted and precipitated prior to use, allowing control over the

amount of cDNA input. This flexibility is useful when working with genes of variable abundance or on

challenging sequences. Any residual cDNA is also available for future amplification reactions of other genes, or

even for other applications. The two-step method is particularly useful when the goal is to detect multiple

targets from a single sample, or to perform multiple PCR amplifications from a single sample.

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Superior Fast Analysis

Real-Time PCR is one of the most powerful and sensitive techniques available. The main advantagr of

real-time PCR over traditional end-point PCR is that it allows you to determine the starting template copy number of your DNA or cDNA with accuracy and high sensitivity over a wide dynamic range. Most modern PCR applications are real-time and can be used in diagnostic, agricultural, biotechnology and pharmaceutical research.

Biomarker discovery and validation

Cancer risk assesment Cellular mRNA and

miRNA

ChIP Detection of

extremely low copy targets

DNA damage measurement

Drug therapy efficacy Gene dosage determination

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Pathogen detection Quantification Viral load

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REAL TIME PCR

SensiFAST™ cDNA Synthesis Kit Cat No. Size BIO-65053 50 Reactions BIO-65054 250 Reactions Features & Benefits • Reproducible – unique reverse transcriptase and TransampTM

buffer generate consistent first-strand cDNA • Broad dynamic range - ideal for dilute and low copy

samples • Sensitive – real-time RT-PCR analysis from as little as 1pg of

starting total RNA • Unbiased cDNA synthesis – complete 5‟ to 3‟ RNA sequence

representation To complement the SensiFAST™ Probe and SYBR® Kits, Bioline has developed the SensiFAST™ cDNA Synthesis Kit to provide a rapid and sensitive method for first-strand cDNA synthesis. The SensiFAST™ cDNA Synthesis Kit displays excellent linearity across a wide range of starting material revealing the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in real-time PCR studies. The novel TransAmp buffer also employs a unique blend of random hexamers and anchored oligo dT to ensure unbiased 3‟ and 5‟ coverage and reverse transcription of alla regions.

SensiFAST™ SYBR® Kit SensiFAST™ SYBR® Kits are a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensuring that the SensiFAST SYBR® Kits produces fast, highly-specific, reproducible and ultra-sensitive real-time PCR. For ease-of-use and added convenience, the kits are provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilisers and enhancers Features & Benefits • Accurate quantification - hot-start capability reduces primer-

dimer formation and saves time • Sensitive - from low copy targets • Rapid - unique buffer chemistry for highest specificity and

sensitivity • Flexible - compatible with all standard and fast cycling

instruments The addition of the latest advances in buffer chemistry and enhancers also ensures that the The SensiFAST™ SYBR® kit produces faster (under 30 minutes), highly reproducible real-time PCR results. Cat No. Product Size BIO-98005 SensiFAST™ SYBR No-Rox Kit 500 rxns BIO-98020 SensiFAST™ SYBR No-Rox Kit 2000 rxns BIO-92005 SensiFAST™ SYBR Hi-Rox Kit 500 rxns BIO-92020 SensiFAST™ SYBR Hi-Rox Kit 2000 rxns BIO-94005 SensiFAST™ SYBR Lo-Rox Kit 500 rxns BIO-94020 SensiFAST™ SYBR Lo-Rox Kit 2000 rxns BIO-96005 SensiFAST™ SYBR & Fluorescein Kit 500 rxns BIO-96020 SensiFAST™ SYBR & Fluorescein Kit 2000 rxns

SensiFAST™ Probe Kit SensiFAST™ Probe has been developed for fast, highly specific and reproducible real-time PCR and has been validated on all commonly used real-time instruments. The kit has been formulated for use with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that the SensiFAST Probe Kit delivers fast, reproducible, highly-specific and ultra-sensitive real-time PCR, making SensiFAST Probe ideal for multiplexing. Features & Benefits • Highly specific – minimal non-specific activity leading to

better efficiency • Ultra-sensitive – perfect for low copy number samples • Fast - unique buffer chemistry allowing fast protocols and

higher productivity • Efficient multiplexing – no loss in efficiency using multiple

probes

The advanced buffer chemistry and enhancers also make SensiFAST™ Probe perfect for multiplexing, allowing more samples to be run in a day with the highest confidence, ideal for high-throughput assays. Cat No. Product Size BIO-98005 SensiFAST™ SYBR No-Rox Kit 500 rxns

BIO-98020 SensiFAST™ SYBR No-Rox Kit 2000 rxns BIO-92005 SensiFAST™ SYBR Hi-Rox Kit 500 rxns BIO-92020 SensiFAST™ SYBR Hi-Rox Kit 2000 rxns BIO-94005 SensiFAST™ SYBR Lo-Rox Kit 500 rxns BIO-94020 SensiFAST™ SYBR Lo-Rox Kit 2000 rxns BIO-96005 SensiFAST™ SYBR & Fluorescein Kit 500 rxns BIO-96020 SensiFAST™ SYBR & Fluorescein Kit 2000 rxns

«Very good linearity right down to 10 copies, very good correlation coefficient, good qPCR reaction efficiency and gave a single band on an agarose gel»

King‟s College London, UK

«With SensiFAST I was able to dramatically boost my efficiencies and increase reproducibility. In addition, I found that multiple primer pairs produced cleaner products with improved melting curves»

UC Davis School of Veterinary Medicine, USA


SensiFAST™ Probe One-Step Kit SensiFAST™ Probe One-Step Kits have been developed for fast RT-qPCR and has been validated on all commonly used real-time instruments. The kit is designed for superior sensitivity and specificity with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes. The SensiFAST Probe One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube and uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR. This also gives SensiFAST Probe One-Step unbeatable efficiency in multiplexing. SensiFAST Probe One-Step Kits consists of a 2x SensiFAST Probe One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor. Features & Benefits • Rapid - optimized for fast reverse transcription real-time PCR • Accurate quantification - for calculating gene expression

from RNA • Sensitive – unique buffer chemistry gives superior sensitivity of

low copy targets • Flexible - compatible with all standard and fast cycling

instruments

The latest advances in buffer chemistryand enhancers, together with high-affinity antibodies ensure fast, ultra-sensitive and

highly-specific one-step real-time RT-PCR. This also gives the SensiFAST™ Probe One-Step Kit unmatched efficiency in multiplexing. Cat No. Product Size BIO-72001 SensiFAST™ SYBR One-Step No-Rox Kit 100 rxns BIO-72005 SensiFAST™ SYBR One-Step No-Rox Kit 500 rxns BIO-92005 SensiFAST™ SYBR One-Step Hi-Rox Kit 100 rxns BIO-92020 SensiFAST™ SYBR One-Step Hi-Rox Kit 500 rxns BIO-94005 SensiFAST™ SYBR Lo-Rox One-Step Kit 100 rxns BIO-94020 SensiFAST™ SYBR Lo-Rox One-Step Kit 500 rxns

SensiFAST™ Genotyping Kits Single nucleotide polymorphisms (SNPs) are single-base variations at specific sites in the genome. The frequency of SNPs is about one in 300bp in the human genome, making it one of the most frequent sequence variations. High resolution melt (HRM) and duel probe-based analysis, are examples of methods used for SNP genotyping. SensiFAST HRM Kit is a post-PCR analysis method of characterizing nucleic acid samples based on DNA strand dissociation behaviour during transition from double-stranded DNA to single-stranded DNA with increasing temperature and is low throughput. SensiFAST Genotyping Kit uses a dual-labeled probe assay fast, precise and highly reproducible genotyping and can be used for high throughput. Features & Benefits

• Accurate allele calling – clear allele discrimination for higher confidence genotype calling

• Tight clustering, easy analysis – perfect for difficult SNPs • Optimized - Assays quickly and reliably established, even

with difficult genomic loci • Cost effective - Ideal for large scale genotyping projects

Cat No. Product Size BIO-34002 SensiFAST™ Lo-Rox Genotyping Kit 200 rxns BIO-34005 SensiFAST™ Lo-Rox Genotyping Kit 500 rxns BIO-34020 SensiFAST™ Lo-Rox Genotyping Kit 2000 rxns BIO-35002 SensiFAST™ Hi-Rox Genotyping Kit 200 rxns BIO-35005 SensiFAST™ Hi-Rox Genotyping Kit 500 rxns BIO-35020 SensiFAST™ Hi-Rox Genotyping Kit 2000 rxns BIO-32002 SensiFAST™ HRM Kit 200 rxns BIO-32005 SensiFAST™ HRM Kit 500 rxns BIO-32005 SensiFAST™ HRM Kit 2000 rxns

SensiFAST™ SYBR® One-Step Kit SensiFAST™ SYBR® One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The SensiFAST SYBR® One-Step Kits uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR. SensiFAST One-Step Kits consist of a 2x SensiFAST SYBR® One-Step mix, plus separate reverse transcriptase and RiboSafe RNase Inhibitor. Features & Benefits • Accurate quantification - hot-start capability reduces primer-

dimer formation and saves time • Sensitive - from low copy targets • Rapid - unique buffer chemistry for highest specificity and

sensitivity • Flexible - compatible with all standard and fast cycling

instruments

The addition of the latest advances in buffer chemistry and enhancers also ensures that the SensiFAST™ SYBR® One-Step Kit produces fast, highly specific one-step real-time RT-PCR. Cat No. Product Size BIO-72001 SensiFAST™ SYBR One-Step No-Rox Kit 100 rxns BIO-72005 SensiFAST™ SYBR One-Step No-Rox Kit 500 rxns BIO-92005 SensiFAST™ SYBR One-Step Hi-Rox Kit 100 rxns BIO-92020 SensiFAST™ SYBR One-Step Hi-Rox Kit 500 rxns BIO-94005 SensiFAST™ SYBR Lo-Rox One-Step Kit 100 rxns BIO-94020 SensiFAST™ SYBR Lo-Rox One-Step Kit 500 rxns BIO-96005 SensiFAST™ SYBR & Fluorescein One-Step Kit 100 rxns BIO-96020 SensiFAST™ SYBR & Fluorescein One-Step Kit 500 rxns

«SensiFAST Probe One-Step Kit has provided our lab with a fast, reliable and economic solution for our viral testing qPCR assays. Our lab routinely uses a quadruplex reaction and SensiFAST is the only mix where we achieve the same results as with singleplex reaction»

Center for Vectorbourne Diseases, UC Davis, USA

«SensiFAST Probe One-Step Kit was used with human parainfluenzavirus type 4 to show sensitivity of down to 10 copies per reaction across an 8 log10 dynamic range»

University of Queensland, Australia


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SINGLE MOLECULE RNA FISH

Single Molecule RNA FISH Stellaris® RNA FISH is a RNA visualization method that allows simultaneous

detection, localization, and quantification of individual mRNA molecules at the cellular level in fixed samples. This novel RNA FISH technology represents a fast and easy-to-use method to achieve conclusive results through compelling images of RNA expression.

Stellaris RNA FISH: Detection, Localization, and Quantification of RNA at the

(Sub)Cellular Level

What is Stellaris RNA FISH?

Stellaris® RNA FISH (fluorescence in situ hybridization) is a RNA visualization method that allows simultaneous

detection, localization, and quantification of individual RNA molecules at the sub-cellular level in fixed samples

using widefield or confocal fluorescence microscopy. A set of Stellaris RNA FISH Probes comprises multiple

oligonucleotides with different sequences each with a fluorescent label that collectively bind along the same

target transcript to produce a punctate signal.

The Stellaris RNA FISH technology follows a simple protocol, does not use exotic reagents, is inexpensive and

platform-independent. It also offers same-day results, and is versatile both in terms of sample type and

application. Stellaris RNA FISH probes may even be labeled with several different dyes, to enable simultaneous

multiplex detection of different RNA targets. Finally, scientists can address the stochastic nature of gene

expression and visualize RNA through direct detection without isolation, purification, and amplification.

Stellaris Protocol

The Stellaris RNA FISH protocol is comparatively simple, and consists of four steps, as shown in the image below.

No exotic reagents are required, and the entire process can be completed in less than a day.

The Stellaris RNA FISH Method

Step 1: Prepare Sample

The sample is grown on or adhered, or sectioned onto a #1 coverglass and permeabilized with 70% ethanol.

Slight variations in sample preparation between different organisms and sample types are covered in the

online protocols. No protease is required.

Step 2: Hybridize Probes

For most samples, hybridization can be completed in 4 hours at +37 °C in a generic laboratory incubator.

Step 3: Wash Sample

After hybridization, wash buffers with short incubation periods are used to remove excess probes. The total time

for this step is 1 – 1.5 hours.

Step 4: Image Sample

At this point, the sample can be imaged using a standard fluorescence microscope. We recommend using a

widefield fluorescence microscope though confocal microscopy can also be used with Stellaris RNA FISH

probes.


Stellaris FISH Protocol

Sensitivity & Specificity

The large number of probes in a Stellaris RNA FISH assay ensures both high sensitivity and specificity. The

inherent redundancies of this direct detection method minimizes the possibility of false negative and false

positive signals. Positive signal identified from the combined localized fluorescence of multiple probes. Off-

target binding of single probes generates only weak and diffuse fluorescence, well below the threshold for

detection of the specifically-targeted mRNAs. Furthermore, Stellaris RNA FISH probes can bind to partially

degraded target mRNAs making them well-suited for formalin-fixed, paraffin-embedded (FFPE) tissue samples.

Multiplexing

Stellaris RNA FISH probes are available with a wide variety of fluorophores, including Biosearch Technologies‟

CAL Fluor® and Quasar® dyes and can be used in a multiplex assay. The dye choices in a multiplex assay are

largely determined by the filters available to a researcher, but it is certainly possible to design an assay

including a DAPI nuclear stain and three Stellaris RNA FISH probe sets labeled with reporter dyes with minimal

spectral overlap.

Multiplex assays can be used to identify a correlation in expression levels between multiple genes, run a control

probe set of known expression with a target simultaneously, or simply save time over running the assays one at

a time. For human cells, we offer inventoried probe sets suitable for use as positive controls. These foundation

gene probe sets are labeled with Quasar® 570 dye.

Stellaris RNA FISH can also be combined with existing technologies such as qPCR, DNA FISH, IHC

(immunohistochemistry), and western blotting to provide complementary information.

Visit www.biosearchtech.com for more informations

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CUSTOM OLIGOS AND PROBES

Custom Oligos and qPCR Probes Together with our higly valuable partners we are proud to provide high quality

and tailored oligonucleotides and qPCR probes. Giving you the opportunity to save money and raise the quality of your daily PCR experiments. Here below, find a list of some of the synthesis products we can provide. For ordering information please contact [email protected]

What we are offering:

DNA Oligonucleotides (minimum purification RP-CARTRIDGE):

Unmodified Oligos - Internal and External modifications - Linker and Spacers - Haptens - Phosphorylation - Dyes -

Redox Modification - Wobbles - Conjugate

RNA Oligonucleotide:

Standard Unmodified or Modified RNA - 2' modified RNA - Internal modification

Special Nucleic Acids:

L-DNA - L-DNA - PNA Oligomers - RNase Substrates

Dual-Labeled BHQ Probes:

Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher

covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released

through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's

hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence

and quantifying the amount of specific target sequences.

BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for

gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities

can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered.

Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference.

BHQplus Probes:

The BHQplus® probe is a novel compact probe for qPCR, modified with a duplex stabilizing technology and

terminated with Biosearch‟s Black Hole Quencher® dye. What differentiates the BHQplus probe from other

traditional probes is that it permits the design of shorter oligonucleotides, typically 15 - 25 bases in length, for

detecting more difficult target sequences. The enhanced specificity of BHQplus probes make them perfect for

genotyping single nucleotide polymorphisms.

BHQplus probes are offered in two convenient deliveries. For BHQplus probes and primers delivered in individual

tubes select just “BHQplus probes”, however if you would like the probes and primers blended into a single tube

select “ValuMix Assay for SNP genotyping”.

ValuMix™ Assays for SNP genotyping comprises two custom BHQplus® probes and two primers combined

within a single tube. This formulation minimizes pipetting error and simplifies your protocol by providing mixed

oligonucleotides ready for SNP genotyping.


Exosomes Exosomes are small lipid spheres around 40-150nm in diameter that form within

the cell. Exosomes contain and protect various RNA and protein cargos which are secreted into the blood and other bodily fluids. Cell Guidance Systems is building a range of innovative products to support your exosome research. The TRIFic™ exosome assay allows you to easily measure exosomes, even in unpurified samples such as blood plasma and cerbrospinal fluid. Exo-spin™ exosome purification kits are now established in many labs and provide a convenient method of preparing purified exosomes from a variety of samples.

Exo-spin™ kits use a unique combination of precipitation and size-exclusion to

purify exosomes. This economical approach yields unparalleled exosome yield

and purity. Exosome precipitation solutions alone leave a large amount of non-

exosomal proteins in samples. Exo-spin™ kits are suitable for the preparation of

pure exosomes from a variety of biological fluids including blood plasma/sera, cell

culture media, urine, saliva. Kits are available for 24 and 48 samples and in mini-

spin column and midi drip-column sizes. Exo-spin™ kits offer excellent quality and

value-for-money.

Choosing Exo-spin™ products

The final step of Exo-spin™ uses size-exclusion and has been calibrated to purify particles in the range 30 –

400nm; the same size range achieved with ultracentrifugation. This range covers all commonly accepted

definitions of exosome size. Exo-spin™ technology is superior to techniques which rely solely on precipitation

which result in co-purification of large amounts of non-exosomal proteins and other material as well as carry

over of the precipitant.

Exo-spin™ provides various configurations and options for use. The basic protocol requires precipitation

followed by further purification by size exclusion column chromatography. In cases where exosome

concentrations are high, the precipitation step may be omitted. Indeed, eliminating the precipitation step is

essential for applications such as mass spec. which will not work in the presence of any precipitant. For blood

samples, exosome concentrations are typically of the order of 1x1012 particles per ml and precipitation is

therefore not usually required. Direct column purification of exosomes from blood samples, without the need for

any precipitation, can rapidly be achieved using product EX03 (for samples up to 0.1 ml) and product EX04 (for

samples up to 1 ml).

Cell culture typically yields of 1x109 particles or less. Use of cell culture devices such as the Integra CELLine flask

can increase the concentration of exosome in the media by around 10-fold, obviating the need for

precipitation. When processing of larger sample volumes is required, precipitation buffer concentrates

exosomes prior to column purification. Use EX01 for cell culture, urine, saliva etc and EX02 for blood.

Whatever the sample and whatever the application, there‟s an Exo-spin™ product for your needs. If you need

any help selecting your product, please contact us and we‟ll get straight back to you.

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EXOSOMES

Application

Sample Type RNA Mass Spec

Antibody-based e.g. Western

blotting, ELISA

Particle analysis e.g. ZetaView,

Nanosight Functional studies

Cell Culture (1 ml – 50 ml)

EX01 N/A EX01 EX01 EX01

Concentrated exosomes in cell

culture to max 1ml (see note 1)

EX03 (to 0,1ml sample), X04 (to 1ml sample) see

note 2


note 2


note 2


note 2


note 2

Large volume cell colture

(50 ml – 500 ml)


Saliva/urine (up to 50 ml)


Blood sera (0,5ml) or blood plasma

(0,25ml)

EX02 EX03 (Sample size 0,1ml) see note 2

EX02 EX02 EX02

Blood sera (1ml) EX04 EX04 EX04 EX04 EX04

Note 1: For example using CELLine from Integra

Note 2: Precipitants adversely affect mass spec analysis. In this case, samples are added directly to column without the need for any precipitation

Find an Exo-spin product that is right for your needs

Exo-spin Precipitation Ultracentrifugation

YIELD +++ ++ ++

PURITY

Highest purity. Non-exosomal proteins are removed by the

column. Any precipitation reagent (required for cell culture

samples) is removed during column purification step.

Extremely low levels of rRNA contamination in RNA. Exosome

size profile closely matches ultracentrifugation

Precipitation reagent co-precipitates non-exosomal proteins. There is no step for removing precipitant or co-

precipotated proteins. High levels of rRNA contamination. Exosome

size profile differs from ultracentrifugation

High purity. High levels of rRNA contamination

SPEED

Very fast to fast (10 minutes – 3

hours) depending on sample type. Sera samples can be

purified in less than 10 minutes

Fast to slow (90 minutes to overnight) depending on sample

type

Slow. Requires overnight centrifugation

COST Low per sample cost for small and

large samples

Relatively high per sample cost for large volume samples. Low

cost for small volumes

Low consumable cost, but high equipment start up and running

cost

Exo-spin Kit Cat No. Product EX01-8 Exo-spin™ kit, 8 columns / 100 ml total sample volume EX01-25 Exo-spin™ kit, 24 columns / 480 ml total sample volume EX01-25L Exo-spin™ kit, 24 columns / 960 ml total sample volume EX01-50 Exo-spin™ kit, 48 columns 960 ml total sample volume

Exo-spin blood Kit Cat No. Product EX02-8 Exo-spin™ blood kit, 8 columns EX02-25 Exo-spin™ blood kit, 24 columns EX02-50 Exo-spin™ blood kit, 48 columns

Visit www.cellgs.com for more informations


Molecular Biology Miscellaneous Reagents We have selected among thousands of reagents a shortlist of must haves!

Ask us at [email protected] for further details.

Molecular Weight Ladders Bioline offers a wide range of Molecular Weight Markers with Loading Buffer in a single tube, enabling accurate DNA sizing. Optionally, the HyperLadders can be used for mass determination of DNA. Each ladder includes one, two or three higher-intensity reference bands for easy identification and orientation. The ready-to-use format minimises the time spent thawing, diluting and adding tracking

dye to the DNA Ladder. Simply transfer the Ladder from the vial to the gel. An additional 5x Sample Loading Buffer is supplied for your convenience. EasyLadders contain all the features of our HyperLadder range, but are designed for short runs (1 to 3cm) in standard or high-throughput agarose gels, providing a fast way to determine the size of DNA fragments.

HyperLadder™ 1kb HyperLadder™ 50bp HyperLadder™

100bp HyperLadder™ 25bp EasyLadder I

BIO-33053 -100 Lanes



BIO-33054 - 100 Lanes

BIO-33039 - 200 Lanes

BIO-33040 - 500 Lanes

BIO-33056 - 100 Lanes

BIO-33029 - 200 Lanes

BIO-33030 - 500 Lanes

BIO-33057 - 100 Lanes

BIO-33031 - 200 Lanes

BIO-33032 - 500 Lanes

BIO-33045 - 200 Lanes

BIO-33046 - 500 Lanes

WWW.AUROGENE.EU

MOLECULAR BIOLOGY MISCELLANEOUS REAGENTS

dNTPs Since its foundation in 1992, Bioline has been actively involved in the development and manufacture of ultra-

pure deoxynucleotides (dNTPs). The state-of-the-art enzymatic manufacturing processes and facilities,

combined with stringent quality assurance and control systems, enable us to manufacture dNTPs with the highest

level of quality and performance, crucial for sensitive techniques and critical applications in molecular biology.

Bioline‟s ultra-pure dNTPs are ideally suited for even the most demanding applications, such as PCR with high

GC-rich DNA. Stable secondary structures in GC-rich templates not only cause resistance to melting, but cause

DNA polymerases to stall, resulting in incomplete amplification products.

Bioline‟s ultra-pure dNTPs posses the highest level of purity in the industry and have been extensively validated for

use in a wide variety of molecular biology applications.

Bioline‟s ultra-pure dNTPs have the distinct advantage of being supplied as lithium salts in highly purified water at

pH 7.5 to ensure funtrionality and long-term stability.

PRODUCT CONC. PACK SIZE CAT NO.

dNTP Individual

dATP 25µmol 100mM (1 x 250µl) BIO-39036

dCTP 25µmol 100mM (1 x 250µl) BIO-39038

dGTP 25µmol 100mM (1 x 250µl) BIO-39037

dTTP 25µmol 100mM (1 x 250µl) BIO-39039

dUTP 25µmol 100mM (1 x 250µl) BIO-39035

HMdCTP 25µmol 100mM (1 x 250µl) BIO-39046

dNTP Set (dATP, dCTP, GTP, dTTP)

dNTP Set

100mM total 4 x 25µmol (4 x 250µl) BIO-39025

100mM total 4 x 100µmol (4 x 1ml) BIO-39049

100mM total 4 x 100µmol (4 x 4 x 250µl) BIO-39026

dNTP Mix (dATP, dCTP, dGTP, dTTP)

dNTP Mix

10mM total 10µmol (1ml) BIO-39044

40mM total 20µmol (1 x 500µl) BIO-39043


10mM total 100µmol (10 x 1mll) BIO-39053


dUTP Mix (dATP, dCTP, dGTP, dUTP)

dUTP Mix 50mM total 25µmol (1 x 500µl) BIO-39041


D-1 Agarose with Low EEO Features

• Extraordinary mechanical resistance for more reliable and

easier handling. • Possibility of varying pore size in accordance with particle

size by modifying the gel concentration. • Easy preparation of the gel by simple dilution in aqueous

buffers either by standard boiling or microwaving. • Greater thermal stability due to high hysteresis (difference

between gelling and melting temperatures). • Excellent transparency of the gel & visibility. • Exceptionally low absorption of staining agents. • Absence of toxicity (the alternative is polyacrylamide which

can be toxic). Applications • High electrophoresis mobility. • Nucleic acid analytical and preparative electrophoresis. • Blotting. • Protein electrophoresis such as radial immunodiffusion. Cat No. Product Size AU1103 D1- Agarose with Low EEO 500g

GelRed Nucleic Acid Gel Stain, 10,000X in water GelRed™ is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed™ is far more sensitive than EB without requiring a destaining step. GelRed™ and EB have virtually the same spectra, so you can directly replace EB with GelRed™ without changing your existing imaging system. GelRed™ can be used to stain dsDNA, ssDNA or RNA in agarose gels via either precast or post gel staining. GelRed™ can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gels via

post gel staining. Features & Benefits • Safer than EB: Shown by the Ames test and other tests to be

nonmutagenic and noncytotoxic • Easy disposal: Passed environmental safety tests for direct

disposal down the drain or in regular trash • Ultra-sensitive: Much more sensitive than EtBr and SYBR® Safe • Extremely stable: Available in water, stable at room

temperature for long-term storage and microwavable • Simple to use: Very simple procedures for precast or post-

electrophoresis gel staining • Compatible with a standard UV transilluminator : Replaces

EtBr with no optical setting change • Compatible with downstream applications : Compatible with

gel purification, restriction digest, sequencing and cloning Cat No. Product Size 41003 GelRed Nucleic Acid Gel Stain 0,5ml

Together with our partners we can provide the following

services: • RNA Sequencing

• Whole Genome Sequencing

• Exome Sequencing

• Recombinant Lentivirus

• Recombinant Adenovirus

• Recombinant Retrovirus

• Custom Cloning

• Site Directed Mutation

• Gene Synthesis

• Peptide Synthesis

• Cell Immortalization

• Stable Cell Line Generation

• CRISPR Stable Knockout/Knock-in Cell Line

• CRISPR-Cas9 Cell Engineering Service

• Protein Expression

• Custom polyclonal AB Production

• Custom monoclonal AB Production And many more….

Custom Services Trust us even for those important custom services for your everyday lab activities. Ask us at [email protected] for further details.

Aurogene s.r.l.

Via dei Lucani 51, 53, 55 - 00185 Roma

Phone: +39.06.9818 5510 Fax: +39.06.8928 0412

Email: [email protected]

Web: www.aurogene.eu

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