molecular imaging system
DESCRIPTION
Molecular Imaging System. General Layout. 600 – 900 nm light is used to illuminate fluorescent substances injected into the subject on the image plane. Optigrid Functionality. A portion of the OptiGrid at 200x. y. Motors provide 120 o phase shifts and focus - PowerPoint PPT PresentationTRANSCRIPT
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Molecular Imaging System
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General Layout
600 – 900 nm light is used to illuminate fluorescent substances injected into the subject on the image plane.
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Optigrid Functionality• Motors provide 120o phase shifts and focus
– Grid moves on the x-axis 11.11 um to complete a phase change
– Improves Clarity– Grid moves 2 mm on the z-axis to focus image– 30 line pair/mm on a 10x10 mm square (line pair
approx .001 in wide)– Lines spatially modulate the illumination
A portion of the OptiGrid at 200xy
xz
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Image Sharpening
Provided by Qioptiq
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3D Rendering
Provided by Qioptiq
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Subsystem BreakdownImaging Unit
Mechanical
Lens Mount Grid Mount
Software
Motor Control
Image Capture
Image Rendering
Optical
Lens System
Medical Testing
Phantom Hand
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Optical System Review
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Lens Selection Parameters
• Focal Length• Magnification• Box Length = 250 mm• Box Height = 200 mm.• Lens size
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Computar C mount
Alternative Lenses:
•Telecentric Lens
•Zoom Lens
•Simple Lens
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OSLO Simulation
Simulation by Jeff
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Rear Projection
45 degrees
90 degrees
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440mm Platen to Lens3mm Lens to Grid
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Degrees of Motion
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Motion Solution
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By Edmund
Construction Aluminum/Stainless Steel
Mounting Surfaces Precision ground
Straight Line Accuracy 2µm/25mm of travel
Position Repeatability ±0.00005"
Configurations X; solid top or thru hole
Drives Metric Micrometer: 0.01mm graduations/0.5mm coarse Fine Screw: no graduations/64 pitch
Load Capacity 3 kg normal (horizontal orientation, force against stage)4.5 kg Ta (vertical orientation, thrust against drive)1.4 kg Tb (vertical orientation, thrust away from spring)
Weight 0.078 kg / axis
NT56-35 by Edmund
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Metric Rotary Stages by Edmund
Stock No. #55-028
Coarse Rotation 360°
Total Fine Rotation ±5°
Total Travel/Knob Rotation 70.2 min
Horizontal Load Capacity 3 kg
Weight 90g
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250mm
250mm
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# Part DescriptionQuantity Vendor
Vendor Part # Price
1 Carestream Box 1 Carestream
2 OptiGrid 1 QiOptiq
3 Dovetail Optical Rail 12" Optical Rail 1Edmund Optics NT54-401 99.75
4 OptiGrid Holder 1 QiOptiq 374352-000
5 Three-Screw Ring Mounts 95mm Adjustable Len Mount 12.5-76mm 1Edmund Optics NT36-605 49.00
6 Small Carrier .5" Dovetail Carrier 2Edmund Optics NT54-403 55.00
7 Metric Rotary Stage 60mm 360 Rotary Stage 2Edmund Optics NT55-028 231.00
8 1/4-20 ScrewsTo screw into rails for mounting grid & lens
9 1/16" Black Polyurethane 80A Durometer, 6" x 6" 1 McMaster 8716K153 11.74
10 Loctite 404 Quick Set (46551) rubber adhesive, 1/3 oz bottle 1 Ellsworth 46551 17.69
11 Rule printed sticker 12" ruled tape 1 McMaster
12 Camera Lens Computar C 1Edmund Optics 90.00
13 Micro Rotary Stage .75" Rotary Stage 1Edmund Optics NT56-572 260.00
14 Aluminum Stock
15 Fiber Optic Light Source 1 Dolan Jenner
16 Grid Transparency 1 Output City -
17 Linear Stage Optional for moving printed transparency 2 - 4Edmund Optics NT56-357 210.00
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Software System Capabilities • Consist of three separate programs
• Kodak Molecular Imaging Software • Optigrid Motor Control GUI • MATLAB
– Scripts will be developed as necessary for calibration and depth resolved discrimination
• Optigrid Calibration– A system specific calibration process will be developed– Process will rely on manual coordination of three separate programs
• Implement necessary algorithms to achieve depth discrimination, as described by Tromberg et al. in US Publication 2006/0184043
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Objective: To mathematically investigate and invoke the necessary
algorithms to achieve the depth discrimination, as described by Tromberg et al. in US Publication 2006/0184043
Bevilacqua, F., Cuccia, D., Durkin, A., Tromberg, B., “Quantitative analysis and imaging of subsurface heterogeneities using spatially structured illumination,” 2002.
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Phantom Design• Objective: To develop a stable, long-term phantom whose optical
properties can be predetermined and reproduced, at selected wavelengths, of human biological tissues of the hand, over a spectral range between 400 and 1,000 nm.
• Purpose: – Simulate light distributions with a geometry of physical tissue.– Initially testing system designs – Calibration of optical devices.– Create reference measurements of reflective fluorescence.
• How:– Understand of the key physical and biochemical characteristics of
tissue that influence its interaction with light. – Match the absorption coefficient μa, scattering coefficient μs, and the
anisotropic coefficient g(λ)
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Design of Phantom
• 3 components:– Casting material
• Polyurethane
– Absorbing material • phthalocyanin dye (Epolight 6084)
– Scattering agent • Aluminum Oxide (alumina; Al2O3)
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Total attenuation coefficient, as a function of concentration, of 0.954-mm-diameter micro-spheres.