molecular pharming
TRANSCRIPT
Molecular Pharming
Presented by: C.G.O. GaasIntroduction to BiotechnologyChemical Engineering DepartmentCIT-University
How is it done?
History and Definition
Biosafety Issues of Molecular Faming
Examples and its Applications
Advantages and Disadvantages
Molecular Pharming
What is Molecular Pharming?
The use of whole organisms, organs, tissues or cells, or cell cultures, as bio-reactors for the production of commercially valuable products via recombinant DNA techniques.
Difference between Molecular pharming andMolecular farming
Molecular pharming-It is defined as the production of active pharmaceutical substances in genetically modified organisms(GMOs).-Plants are preferred as plants do not carry pathogens. Still the safety of GMO is a concern.-First plant derived pharmaceutical protein is serum albumin.
Molecular farming
It is defined as the use of genetically modifiedorganisms (GMOs) as a production platform forrenewable raw materials, fine chemicals and dietarysupplements
History
• 1986 First plant -derived recombinant therapeutic protein-human GH in tobacco & sunflower. (A. Barta, D. Thompson et al.)
• 1989 First plant -derived recombinant antibody – full-sized IgG in tobacco. (A. Hiatt, K. Bowdish)
• 1990 First native human protein produced in plants –human serum albumin in tobacco & potato. (P. C. Sijmons et al.)
• 1992 First native human protein produced in plants –human serum albumin in tobacco & potato. (P. C. Sijmons et al.)
• 1995 First plant derived industrial enzyme – α-amylase in tobacco. (J.Pen, L. Molendijk et al.)
History
• 1996 First plant derivedprotein polymer – artificial elastin in tobacco. (X. Zhang, D. W. Urry, H. Daniel)
• 1997 First clinical trial using recombinant bacterial antigen delivered in a transgenic potato. (C. O. Tacket et al.)
• 1997 Commercial production of avidin in maize.(E. E. Hood et al.)
• 2000 Human GH produced in tobacco chloroplast.(J. M. Staub et al.)
• 2003 Human GH produced in tobacco chloroplast.(J. M. Staub et al.)
Expression and assembly of a functional antibody in algae Commercial production of bovine trypsin in maize.(S. L. Woodard )
How is it done? A DNA molecule carrying the genetic information for a
pharmaceutical substance is introduced into the plant genome.
This process (1) is called transformation. The genes can be incorporated permanently (stable
transformation) or for a short period of time (transient
transformation). The transformed plant acts as a bioreactor
producing large quantities of the pharmaceutical using its protein making machinery (2). Through
industrial processing, the pharmaceutically active
substance is extracted from the plant (3) and made into in a formulated product (4), for
example a pill.
How is it done?
Virus (left) with genetic material
inside and surface proteins (green and orange) on
the outside. Virus-like particle (on
the right) without genetic material and some virus surface proteins
(green)
Pharming General Strategy• Clone a gene of interest
• Transform the host
platform species
• Grow the host species,
recover biomass
• Process biomass
• Purify product of interest
• Deliver product of
interest
Comparison of different Pharming production systems
Parental therapeutics and pharmaceutical intermediates
Industrial proteins and
enzymes
Molecular Pharming and Farming Applications
Monoclonal antibodies
Antigens for ediblevaccines
Biopolymers
Biosafety Issues on Pharming
• Gene and Protein
Pollution
• Product Safety
Transgene PollutionTransgene pollution is the spread of transgenes beyond the intended genetically-modified species by natural gene flow mechanisms.
Two classes of transgene pollution:• The possible spread of primary
transgenes.• The possible spread of superfluous DNA
sequences.
Transgene Pollution MechanismVertical gene transfer• Vertical gene transfer is the movement of DNA between plants that
are at least partially sexually compatible.• Most prevalent form of transgene pollution.• Occurs predominantly via the dispersal of transgenic pollen/seed
dispersal.Horizontal gene transfer• Horizontal gene transfer is the movement of genes between species
that are not sexually compatible and may belong to very different taxonomic groups.
• The process is common in bacteria (Agrobacterium tumefaciens and related species), resulting in the transfer of plasmid-borne antibiotic resistance traits.
• Antibiotic resistance markers and transgenes encoding pharmaceutical proteins could be acquired by human pathogens.
Product safety
• The purified protein may be contaminated with toxic substances from the plant or applied to the plant, e.g. plant derived metabolites, allergens, field chemicals (e.g. herbicides, pesticides, fungicides), fertilizers, dung and manure.
• The product itself, due to intrinsic properties, may be harmful.
Transgene pollution –possible solutions
• Minimum required genetic modification.• Elimination of non-essential genetic information.• Containment of essential transgenes. (Physical or artificial)
-Maintained in green house-Concealing flowers/fruits in plastic bags in field-Isolation-Barrier crops
• Alternative production systems-transient expression.-Plant suspension cultures in sealed, sterile reactor vessels. (Fischer et al., 1999a; Doran, 2000)
Perspectives on Molecular Pharming
• Use of virus infected plants is best approach for molecular farming
• Molecular farming provides an opportunity for the economical and large-scale production of pharmaceuticals, industrial enzymes and technical proteins that are currently produced at great expense and in small quantities.
• We must ensure that these benefits are not outweighed by risks to human health and the environment
Thank you for listening. . . .