molecular testing for common bacterial stool pathogens to .../media/files...molecular testing for...

1
October 2015 Molecular Testing for Common Bacterial Stool Pathogens to Replace Conventional Stool Culture in 2016 Providence Health & Services Oregon Laboratories is pleased to announce that our conventional stool culture will be replaced with a molecular nucleic acid amplification test starting on January 4, 2016. The test, called Stool Pathogen Panel, NAAT (LAB24176) will provide rapid diagnosis of the following bacterial stool pathogens: - Salmonella spp. - Shigella spp./Entero-Toxicgenic E. coli (ETEC) - Sxt1/stx2 (Shiga toxin-producing E. coli) - Campylobacter jejuni and Campylobacter coli Rationale: The new Stool Pathogen Panel NAAT detects the exact same pathogens as our current stool culture, just using a different technology. It is, however, up to 50% more sensitive than culture 1 . It will also significantly reduce the time to result from several days to ≤ 24 hours 2 . Specimen collection and transportation will not change. The test will be performed at the Regional Microbiology Laboratory only. The cost of the molecular Stool Pathogen Panel NAAT is comparable to that of the current stool culture. Test Name: Stool Pathogen Panel NAAT Epic Test Code: LAB24176 Cerner Lab Test Code: 48551 Collect: Fresh diarrheal, liquid or soft stool in Cary-Blair transport medium Stability (from collection): Ambient: 4 hours, Refrigerated: 3 days, Frozen: 30 days Unacceptable Conditions: Fresh stool, leaking containers Performed: Sunday - Saturday Reported: Within 24 h Methodology: Multiplex Real-Time Polymerase Chai n Reaction (PCR) CPT Code: 87506 Note: Please update your preference list! References: 1. Harrington SM et al. Multicenter evaluation of the BD max enteric bacterial panel PCR assay for rapid detection of Salmonella spp., Shigella spp., Campylobacter spp. (C. jejuni and C. coli), and Shiga toxin 1 and 2 genes. J Clin Microbiol. 2015 May;53(5):1639-47. 2. Mortensen JE et al. Comparison of time-motion analysis of conventional stool culture and the BD MAX™ Enteric Bacterial Panel (EBP). BMC Clin Pathol. 2015 May 28;15:9. For more information, please contact Providence Lab Services at (503) 215-6660, the Regional Microbiology Laboratory at (503) 893-7786, or Dr. Margret Oethinger at (503) 893-7718.

Upload: others

Post on 24-Jul-2020

5 views

Category:

Documents


0 download

TRANSCRIPT

Page 1: Molecular Testing for Common Bacterial Stool Pathogens to .../media/Files...Molecular Testing for Common Bacterial Stool Pathogens to Replace Conventional Stool Culture in 2016 Providence

October2015

Molecular Testing for Common Bacterial Stool Pathogens to Replace Conventional Stool Culture in 2016

Providence Health & Services Oregon Laboratories is pleased to announce that our conventional stool culture will be replaced with a molecular nucleic acid amplification test starting on January 4, 2016. The test, called Stool Pathogen Panel, NAAT (LAB24176) will provide rapid diagnosis of the following bacterial stool pathogens:

- Salmonella spp. - Shigella spp./Entero-Toxicgenic E. coli (ETEC) - Sxt1/stx2 (Shiga toxin-producing E. coli) - Campylobacter jejuni and Campylobacter coli

Rationale: The new Stool Pathogen Panel NAAT detects the exact same pathogens as our current stool culture, just using a different technology. It is, however, up to 50% more sensitive than culture1. It will also significantly reduce the time to result from several days to ≤ 24 hours2. Specimen collection and transportation will not change. The test will be performed at the Regional Microbiology Laboratory only. The cost of the molecular Stool Pathogen Panel NAAT is comparable to that of the current stool culture.

Test Name: Stool Pathogen Panel NAAT

Epic Test Code: LAB24176 Cerner Lab Test Code: 48551 Collect: Fresh diarrheal, liquid or soft stool in Cary-Blair transport medium Stability (from collection): Ambient: 4 hours, Refrigerated: 3 days, Frozen: 30 days Unacceptable Conditions: Fresh stool, leaking containers Performed: Sunday - Saturday Reported: Within 24 h Methodology: Multiplex Real-Time Polymerase Chai n Reaction (PCR) CPT Code: 87506

Note: Please update your preference list! References:

1. Harrington SM et al. Multicenter evaluation of the BD max enteric bacterial panel PCR assay for rapid detection of Salmonella spp., Shigella spp., Campylobacter spp. (C. jejuni and C. coli), and Shiga toxin 1 and 2 genes. J Clin Microbiol. 2015 May;53(5):1639-47.

2. Mortensen JE et al. Comparison of time-motion analysis of conventional stool culture and the BD MAX™ Enteric Bacterial Panel (EBP). BMC Clin Pathol. 2015 May 28;15:9.

For more information, please contact Providence Lab Services at (503) 215-6660, the Regional Microbiology Laboratory at (503) 893-7786, or Dr. Margret Oethinger at (503) 893-7718.