MONITORING PROGRESS MONITORING PROGRESS

REPORT 2006REPORT 2006

Food and Agriculture (FA) Domain Food and Agriculture (FA) Domain

CommitteeCommittee

3rd DC meeting, Antalya (TR), 31 Jan – 2 Feb 2007

COST Action 871Domain Food and Agriculture (FA)

Cryopreservation of crop species in Europe

CSO approval date 27/06/2006

Entry into force 20/09/2006

End date 11/12/2010

Participating Countries : 15

BG, CZ, DK, FI, FR, BG, CZ, DK, FI, FR, DE, IT, NL, PL, PT, DE, IT, NL, PL, PT, CS, SK, ES, UK CS, SK, ES, UK

In the pipe-lineIn the pipe-line: : TR, RO, GR, LUTR, RO, GR, LU

Non-COST: Vavoliv, RussiaVavoliv, Russia Economic Dimension: 38 Million €

Chair : BE

Duration: 4 years

Action 871: Cryopreservation of crop species in Europe

Management Report data, part 2 Management Report data, part 2 Chair of the Action:

Dr. Bart Panis: Laboratory of Tropical Crop Improvement, K.U.Leuven,Kasteelpark

Arenberg 13, B-3001 Leuven, BELGIUM Vice chair of the Action:

Professor Paul LYNCHBiological and Forensic Sciences department,,School of Science University of Derby, Kedleston Road DE22 1GB Derby, United Kingdom

Rapporteur:Dr. Charles Spillane:

Genetics & Biotechnology Lab, Biochemistry DepartmentUniversity College Cork, Lee Maltings 2.1, Cork, Ireland

Science Officer:B. STOL

Administrative Officer: C. PEETERS Action website: in preparation

OBJECTIVES

The main objective of this action is to improve and apply technologically advanced techniques for plant genetic resources conservation of crops that are grown/ and or conserved in Europe with main emphasis on long-term conservation through cryopreservation.

Management Report data, part 3 Management Report data, part 3

Objective 1: To screen in detail the current utilization of plant cryopreservation in Europe.

Objective 2: To screen and compare the efficiency of existing plant cryopreservation protocols.

Objective 3: To improve fundamental knowledge about cryoprotection through the determination of physico-biochemical changes associated with tolerance towards cryopreservation.

Objective 4: To develop new plant cryopreservation protocols. Objective 5: To assure the genetic stability and true-to-typeness of

plants after cryopreservation. Objective 6: To apply cryopreservation to European plant

germplasm collections. Objective 7: To proof the environmental, social and economic

impact of plant cryopreservation.

Specific objectives of CRYOPLANET ?

Management Report data, part 3 Management Report data, part 3

WG1Fundamental aspects of

cryopreservation/cryoprotection and genetic stability

WG2Technology, application and

validation of plant cryopreservation

Optimisation Feed back

Management Report data, part 4 Management Report data, part 4

1.1. Fundamental aspect of cryopreservation and cryoprotectionElucidation of the physico-biochemical background of cryoprotection and

cryopreservation.

WG1: Fundamental aspects of cryopreservation/cryoprotection and genetic stability

Water thermalbehavior

Cytoskeletalprotein

Membranecomponents

Proteins Sugars

Polyamines

OxidativestressProfessor Pawel PUKACKI (chair WG1)

Physiology of Abiotic Stress Lab

Institute of Dendrology, Polish Academy of Sciences

Parkowa 5, 62-035 Kornik

Poland

1.2. Genetic stability and authenticity Assessment of the genetic integrity of plants to determine if they are ‘true

to type’ after cryopreservation.

WG1: Fundamental aspects of cryopreservation/cryoprotection and genetic stability

Dr. M. Angeles REVILLA BAHILLO (co-chair WG1)

Departamento Biología de Organismos y Sistemas

Faculty of Biologia, Universidad de Oviedo

Catedrático Rodrigo Uría s/n, 33071 Oviedo

Spain

2.1. Technology aspects of cryopreservationApplications of different cryopreservation protocols to different plant

species and tissues.

WG2: Technology, application and validation of plant cryopreservation

Dr. Florent ENGELMANN (chair WG2)

UR 141, IRD

BP 64501, 911 avenue Agropolis

34394 Montpellier cedex 05

France

2.2. Impact and applications of cryopreservation in plantsgenebanks, establishment of cryo-bank and dissemination of results

WG2: Technology, application and validation of plant cryopreservation

Dr. Joachim KELLER (co-chair WG2)

Leibniz Institute of Plant Genetics and Crop Plant Research IPK

Corrensstraße 3, 466 Gatersleben

Germany

•National Seed Storage Laboratory (NSSL) (Fort Collins, Colorado, USA): 2,100 accessions of apple (dormant buds) •National Clonal Germplasm Repository (NCGR) of Corvallis (USA): 104 accessions of pear (shoot tips);•International Potato Centre (CIP) (Lima, Peru) : 345 potato accessions•Tissue Culture BC Research Inc.(Vancouver, BC, Canada) : 5000 accessions representing 14 conifer species•AFOCEL (Association Forêt Cellulose) of France, with over 100 accessions of elm (dormant buds);•National Institute of Agrobiological Resources (NIAR) of Japan, with about 50 accessions of mulberry.•IRD (Montpellier, France) : 80 accessions of oil palm •IPK (Gatersleben, Germany)/ DSMZ (Braunschweig, Germany) : 519 old potato varieties•INIBAP, Laboratory of Tropical Crop Improvement, K.U.Leuven (Heverlee, Belgium) : 500 banana accessions

State of the art (Europe/rest world)

(i) the unavailability of efficient and robust cryopreservation protocols applicable to many plant species and diverse germplasm types

(ii) limited awareness of plant researchers unacquainted to recent developments in cryogenic storage methods

(iii) lack of coordinated research on plant cryopreservation.

COST action like “CRYOPLANET” can make the difference

Why is cryopreservation not more widely applied?

Time table

Year 1 Year 2 Year 3 Year 4

Coordination

Kick-off meeting

Homepage

Reporting

MC meeting

WG1 meeting

WG2 meeting

Workshop*

STSMs

Final Conference

MC meeting: Management committee meeting; WG meeting: Working group meeting; STSMs: Short-term scientific missions; Workshop*: Timing of the Inter-COST Workshops will be defined in agreement with the Management committee of that specific Action.

Meetings plannedMeetings planned1/ WG1 meeting

April 13-14 Oviedo (Spain)Organiser Dr. M. Angeles REVILLA BAHILLO

2/ WG2 meeting combined with meeting of executive committee May 11-12 Firenze (Italy)Organiser Dr. Lambardi MAURIZIO

3/ Management Committee meeting In combination with the Society for Low Temperature Biology Annual Scientific meeting at the University of Derby (12-14th Sept 2007) hosted by Professor Paul LYNCH

4/ Training Workshop on DSC and thermal analysis (8 researchers)End 2007Organiser Dr. Milos FALTUS (Prague, Czech Republic)

• Articles in refereed scientific journals• Common reviews, books• A public website (information about the project, the achievements,

services/consultancies offered and announcements of training workshops.

• Information on the official webpages of the collaborating institutions.

• The consortium will organize workshops for scientists, germplasm curators regulatory bodies and policy makers

• At the end of the Action, the consortium will offer its expertise as a service to the EU.

• Presentations at International Conferences, for promoting the European know-how and increasing the international collaboration.

• Teaching activities in Universities at undergraduate and post-graduate level. Young scientists and engineers will thus be trained and informed on the latest developments in cryopreservation.

Significant need to develop dissemination plan. This will be a major agenda item at Executive

Committee Meeting in May (Firenze). Members of COST 871 need to feedback ideas to the

Executive Committee by end of April 2007

Dissemination plansDissemination plans

ConclusionsConclusions

Everything is in place