mouse acute femoral blood flow

8/3/2019 Mouse Acute Femoral Blood Flow

1/14

Transonic

Systems Inc.

Considerations for Flow Measurement on

the Mouse Femoral Artery

Location of Measurement with the Flowprobe

Vessel Diameter

Maintaining Body Temperature

Experimental Set-up

Photos and data courtesy of Michael F. Callahan

Depts. of Orthopaedic Surgery,

Physiology & Pharmacology,

Wake Forest University Health Sciences

Winston-Salem, North Carolina

All information in this file is privileged information and may

not be used without permission of the author.


2/14

Transonic

Systems Inc.

Femoral Nerve,Artery and Veinexiting fromabdominal wall

Epigastric Nerve,Artery and Vein

Femoral Nerve,Superficial FemoralArtery and Vein

Branches of Femoral Artery Before and After Dissection

ApproximateLocation ofKnee

Transonic

Systems Inc.


3/14

Transonic

Systems Inc.

Femoral artery proximal to the epigastric a., isolated from the

femoral vein, measures ~ 400 m diameter with a micrometer.

Flow measurements can be made in this location easily.

Transonic

Systems Inc.


4/14

Transonic

Systems Inc.

Femoral Blood Flow in Anesthetized Mouse Recorded withTransonic Systems Flowmeter & Nanoprobe

MFCallahan, 3/03Data Courtesy of Michael F. Callahan, WakeForest Health Sciences, Winston-Salem, NCTransonic

Systems Inc.


5/14

Transonic

Systems Inc.

39C

0.82 ml/m

37C

0.64 ml/m

35C

0.51 ml/m

33C

0.27 ml/m

Effect of Core Temperature onFemoral Blood Flow (22g CD-1, 3-16-03)

Data Courtesy of Michael F. Callahan, Wake

Forest Health Sciences, Winston-Salem, NC

Temperature has a profound effect on femoral blood flow and must be monitored.


6/14

Transonic

Systems Inc.

Flow measurements on the femoral arterydistal to the epigastric a.

Isolation of the artery from thevein in this position is verychallenging. There is less fasciaand connective tissue around the

sheath of the artery and vein tomanipulate the vessel free.

The nerve runs immediatelyadjacent and is very easy to tear.

Once isolated, avoid pulling up onthe artery which would causevascular spasm or puttingpressure on the vein to causeocclusion.

Superficial femoral a.


7/14

Transonic

Systems Inc.

The superficial femoral artery, distal to theepigastric a., is isolated from the femoral vein and

measures ~ 200 m diameter on the scale.


8/14

Transonic

Systems Inc.

Transonic 0.5PSB flowprobe on the superficialfemoral artery distal to the epigastric a.


9/14

Transonic

Systems Inc.

32g male CD-1 with 0.5PSB probeon superficial femoral artery

Temperature 33oC.

Michael Callahan, Wake Forest Univ. HealthSciences, 2/5/2004

Sample flow trace from lower on femoral artery nearthe knee. Since the vessel diameter fills < 60% of theprobe lumen, probe position must remain fixed for a

stable measurement.


10/14

Transonic

Systems Inc.

Experimental Setup for Femoral Artery Blood Flow Study

Close up of the Transonicnanoprobe handle clamped

in a micromanipulator withtwo axis adjustment to finetune and stabilize position ofprobe for measurement.


11/14

Transonic

Systems Inc.

Experimental Setup for Femoral Artery Blood Flow Study

Probe holder withmagnetic base,adjustable arm, and

micro-positioningadjustment

Heating pad & lamp used withgauze cover to maintain mousecore temperature. Temp. is

monitored with an anal probe.

TransonicSystems400-SeriesFlowmeterConsole


12/14

Transonic

Systems Inc.

Simultaneous Femoral Artery Blood Flow & Pressure Recording


13/14

Transonic

Systems Inc.

Flow recorded from the superficial femoral a. with occlusion;Pressure recorded from the contra lateral femoral a.


14/14

Transonic

Systems Inc.

Transonic Systems, Inc. gratefully acknowledges the investigators

for the presentation of this data on the Transonic website.All information in this file is privileged information and may

not be used without permission of the authors.

Photos and data courtesy of :Michael F. Callahan, PhD

Thomas L. Smith, PhD

Depts. of Orthopaedic Surgery,

Physiology & Pharmacology,

Wake Forest University Health Sciences

Winston-Salem, North Carolina

Edited by Margo Sosa, Sr. Product Manager

Transonic Systems, Inc.

www.transonic.com

December 2004
http://www.transonic.com/http://www.transonic.com/

mouse acute femoral blood flow

Documents