m.sc. biotechnology programme 2019-20 and syllabus m. sc... · 2021. 1. 7. · m. sc. biotechnology...

M. Sc. Biotechnology SYLLABUS

(Effective from the session 2019-2020)

M.Sc.BiotechnologyProgramme2019-20



M. Sc. Biotechnology Program Objectives The aim of this program is to provide a broad overview of biotechnology, and thus produce graduates with sufficient knowledge and expertise to apply them in basic biosciences research and teaching. The program has following specific objectives:

1. To provide an intensive and in-depth knowledge to the students in diverse areas of basic biotechnology 2. To impart knowledge and skills necessary to generate recombinant DNA and genetically engineered

organisms 3. To provide bioinformatics skills for biological sequence data mining 4. To train the students to take up wide variety of roles like researchers, scientists, and academicians 5. To provide the students hands on training for the technical review and literature search for designing

research problems Programme Learning Outcome (PLO) PLO1: Technical Knowledge: Substantial multidisciplinary knowledge about mathematics, basic sciences, related to specialization for solving various complex scientific problems. PLO2: Development of critical analytical approach in identifying, understanding various problem in the present world, that can be solved with the help of basic scientific knowledge and its applications. PLO3: Ability to contribute towards innovative thinking, scientific approach, and trouble-shooting skills for various problems by utilizing scientific knowledge in accordance with health- environment safety, cultural and social aspects. PLO4: Can independently carry out a complete scientific work process, including the theoretical background, hypotheses generation, collecting and analyzing data as along with the interpretation of results and their presentation PLO5: Critically evaluate appropriate tools and techniques as well as high competency and multidisciplinary experience for obtaining accurate results within limited resources PLO6: Understands the role of biotechnology in society, health related issues, environmental concerns and cultural problems through scientific interventions. PLO7: Assessment of impact specifically on environment and society due to proposed innovation-based solutions and for obtaining sustainable development. PLO8: Should be familiar with the research & professional ethics as well as responsibility taking capability for standard practices. PLO9: has the ability to successfully carry out advanced tasks and projects, both independently and in collaboration with others, and also across disciplines. PLO10: Empower learner’s multiple competencies and adding quality dimension to learner’s knowledge for proper documentation, effective report writing and presentations. PLO11: Inculcate managerial skills specifically finance management, team building capacity, individual approach along with existing scientific multidisciplinary knowledge for handling projects and better-quality outcomes PLO12: Aware of recent scientific updates and advanced technologies for quality work and to fulfill the need of the hour throughout life. Program Specific Outcome (PSO) The aim of this program is to prepare students to take up a career in biotechnology industry or research. The course curriculum is designed to strengthen the fundamentals in basic subjects and provide hands on practice in all the disciplines of biotechnology. PSO1: Fundamental multidisciplinary knowledge will enable students to design, conduct experiment, analyze and interpret data for investigating problems in Biotechnology and allied fields. PSO2: Capability to understand the potentials, and impact of biotechnological innovations on environment and their implementation for finding sustainable solution to issues pertaining to environment, health sector, society etc. PSO3: This course will develop effective communication, managerial and other skills in students to carry out advanced projects and collaborations even across the disciplines. PSO4: Help to evolve with recent innovations and scientific updates in the technological era in accordance with best scientific temperament, professional and research ethics throughout life.



Term Paper-I (MSB1506) removed w.e.f. 2017-18. L - Lecture T -Tutorial P -Practical CIE -Continuous Internal Evaluation ESE -End Semester Exam C - Credit

S. No

Subject Code Subject L T P CIE

ESE Total C

THEORY

1. MSB1006 General Biochemistry 4 0 - 40 60 100 4

2. MSB1007 General Microbiology 4 0 - 40 60 100 4

3. MSB1008 Molecular Biology of the Cell 4 0 - 40 60 100 4

4. MSB1009 Molecular Genetics 4 0 - 40 60 100 4

5. MSB1010 Analytical Techniques in Biotechnology 4 0 - 40 60 100 4

PRACTICAL/TRAINING/PROJECT

6. MSB1504 Biochemistry and Analytical Techniques Lab - - 2 80 20 100 1

7. MSB1505 Cell and General Microbiology Lab - - 2 80 20 100 1

8. MSB1507 Seminar / Journal Club-I - - 1 100 0 100 1

Total 20 0 5 460 340 800 23

M. Sc.: Biotechnology I Year: I Semester



S. No

Subject Code Subject L T P CIE ESE Total C

THEORY

1. MSB2005 Molecular Biology 4 0 - 40 60 100 4

2. MSB2006 Biology of the Immune System 4 0 - 40 60 100 4

3. MSB2007 Enzymology 4 0 - 40 60 100 4

4. MSB2008 Bioinformatics 4 0 - 40 60 100 4

5. MMA2007 Biostatistics 3 0 - 40 60 100 3


6. MSB2506 Molecular Biology and Bioinformatics Lab 0 0 2 80 20 100 1

7. MSB2507 Immune System and Enzymology Lab 0 0 2 80 20 100 1

8. MSB2508 Term Paper-I 0 0 2 100 0 100 1 Total 19 0 6 460 340 800 22

Students will go for summer training during the break between II and III semesters (I and II year). After the completion of the training they will be asked to submit the report that will be evaluated during the III semester.

M. Sc.: Biotechnology I Year: II Semester



S. No

Subject Code Subject L T P CIE ESE Total C

THEORY

1. MSB3007 Genetic Engineering 4 0 - 40 60 100 4

2. MSB3008 Genomics and Proteomics 4 0 - 40 60 100 4

3. MSB3009 Bioprocess Engineering and Technology 3 0 - 40 60 100 3

4. MSB3010 IPR, Bioethics and Biosafety 3 0 - 40 60 100 3

5. MSB3011 Technical writing and Literature survey 2 0 - 20 30 50 2

6. ---- Department Elective-I 4 0 - 40 60 100 4


7. MSB3506 Genetic Engineering Lab 0 0 2 80 20 100 1

8. MSB3507 Bioprocess Engineering and Technology Lab 0 0 2 80 20 100 1

9. MSB3508 Seminar / Journal Club-II 0 0 1 80 20 100 1

10. MSB3509 Summer Training Project Work Review 0 0 2 100 100 100 1

Total 20 0 7 560 390 950 24

M. Sc.: Biotechnology II Year: III Semester



S. No

Subject Code Subject L T P CIE ESE Total

C


1. MSB4503 Major Project - - 20 50 50 100 20

Total - - 20 50 50 100 20

M. Sc.: Biotechnology II Year: IV Semester



General Biochemistry MSB1006

L T P C 4 0 0 4

Course Objective: The course aims is the understanding structure and function of major classes of biopolymers. Aims of the course are to understanding central metabolic process and role of enzymes in modulating pathways. The theoretical background of biochemical knowledge to interpret the results in biochemistry experiments. UNIT I: Properties of water and biological buffers [8 Hours] Some Important Properties of Water: Dissociation of water and its ion product, pH, pka Bronsted Acids, ionization of weak acids and bases; Henderson-Hasselbalch equation, Titration curves and buffering action, physiological buffers; Principles of Thermodynamics UNIT II: Structure, function and metabolism of carbohydrates [8 Hours] Carbohydrates: Definition, nomenclature, classification, structure, chemistry and properties, Storage and structural polysaccharides, Metabolism of carbohydrates; Glycolysis, TCA cycle, Gluconeogenesis, HMP pathway, Glycogen metabolism, Oxidative phosphorylation, Regulation of carbohydrate metabolism. UNIT III: Structure, function and metabolism of lipids [8 Hours] Lipids: Classification, nomenclature and structure of fatty acids, Storage and structural lipid; triacylglycerols, sphingolipids and phospholipids, waxes, glycolopids and sterols; Transport of lipids in blood plasma, lipoproteins, Beta-oxidation of fatty acids, Biosynthesis of fatty acids and triacylglycerols; Regulation of lipid metabolism. UNIT IV: Structure, function and metabolism of proteins [8 Hours] Proteins: Amino Acids: structure and functional group properties, essential and non-essential amino acids; non-protein amino acids, Acid base properties, Biosynthesis and degradation of following amino acids: alanine, serine, lysine cysteine, arginine, methionine, tryptophan, phenylalanine glutamine;. Proteins: peptides, primary, secondary, tertiary and quaternary structure of proteins; Hydrolysis of proteins: Action of different proteases; Regulation of amino acid metabolism. UNIT V: Structure function and metabolism of nucleic acids [8 Hours] Nucleic acids: General structure and functions of purines, pyrimidines, nucleosides and nucleotides; structure of DNA and RNA , Hydrolysis of nucleic acids; Biosynthesis of purines and pyrimidines, nucleosides and nucleotides; Degradation of purines and pyrimidines. TEXT BOOKS: 1. Lehninger, Nelson David. L. and M. Cox Michael, 2004, Principles of Biochemistry, 4th ed.,

Worth Publishers 2. Conn E., Stumpf P., 2009, Outlines of Biochemistry, 5th ed., John Wiley & Sons

REFERENCE BOOKS:

1. Voet D., Voet G. Judith, 2011, Biochemistry, 4th ed., Wiley Zubay G., 1993, Principal of Biochemistry, Brown (William C.) Co, U.S.A.

2. M. Berg Jeremy, Tymoczko L. John and Stryer Lubert, 2004, Biochemistry, 5th ed., Freeman Int. Edition



Course Learning Outcomes (CLO): On completion of this course, the students will be able to: CLO Description Bloom’s Taxonomy

Level CLO1 Understand the concept of pH and biological buffer system

PLO1, PLO2 2

Understanding

CLO2 Explain structure, function and metabolism of bio molecules PLO1, PLO2

1, 2 Remembering, Understanding

CLO3 Explain coordinate regulation of metabolic pathways PLO1, PLO2

2 Understanding

CLO4 Learn to Analyze and apply theoretical knowledge of biochemistry for designing of new biochemistry research project PLO1, PLO2, PLO3, PLO4,PLO5, PLO6, PLO12

2, 3, 4, 6 Understanding,

Applying, Analyzing Creating

Mapping of CLOs with PLOs & PSOs

Course Learning

Outcomes

Program Learning Outcomes (PLOs) Program Specific Outcomes (PSOs)

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CLO1 H M H L M H CLO2 H H H M H H CLO3 M H H M L M CLO4 H H H H M H H H M H L

H: High M: Medium L: Low



General Microbiology MSB1007

L T P C 4 0 0 4

Course Objective: This 4-credit course is designed to give an in-depth view of prokaryotes with special concentration on the structure, metabolism and genetics of bacteria. The students will gain a breath of understanding of microbiology from the cellular to molecular levels of organization in conjunction with bacterial physiology and metabolism. The specific objectives are: 1. To understand the scope and importance of microorganisms, in particular, bacteria, for human beings 2. To understand the central metabolic pathways operating in a bacterial cell for its growth and survival 3. To learn the three genetic recombination techniques vital to bacterial heredity and variation UNIT I: History and scope of microbiology [8 Hours] Major events leading to the establishment of science of microbiology; identification of microorganisms on the basis of morphological, physiological, biochemical, immunological, and molecular characteristics; bacterial classification according to Bergey’s Manual of Systemic Bacteriology; relevance of microbiology to human life; microorganisms as model organisms UNIT II: Structure of prokaryotic cell [8 Hours] Overview of prokaryotic cell (size, shape and arrangement of the cell); structure of bacterial cell wall; structures external to cell wall (capsules, slime layer, pili and fimbriae, flagella, prosthecae); structures internal to cell wall (inclusion bodies, magnetosomes, nucleoid, mesosome); spores and cysts; differences between Gram +ve and Gram –ve bacteria,and archaebacteria and eubacteria UNIT III: Growth and nutrition In Bacteria [8 Hours] Nutritional types in microorganisms on the basis of sources of carbon, energy and electrons/hydrogen; Uptake of nutrients by bacterial cells: Passive diffusion, facilitated diffusion, group translocation; chemiosmotic theory and active transport; iron uptake; growth phases and mathematics of bacterial growth; physical and chemical agents to control bacterial growth UNIT IV: Microbial metabolism [8 Hours] Overview of microbial metabolism; types of photosynthesis in bacteria; photosynthetic machinery of bacteria (bacteriochlorophyll, carboxysomes, bacterial reaction centre, electron transport); photosynthetic fixation of CO2 in bacteria; bacterial fermentation; assimilation of inorganic phosphorus, sulfur, and nitrogen UNIT V: Microbial genetics [8 Hours] Plasmids and episomes; transformation (Griffith’s experiment, competent cells); conjugation (U-tube experiment; F+ X F-mating; Hfr conjugation, F’ conjugation); transduction (generalized and specialized transduction); transposable elements in bacteria (IS elements) TEXT BOOKS:

1. Ananthanarayan, R and Kapil, A. 2013. A & P Textbook of Microbiology. 9th ed. Orient BlackSwan

2. Pelczar, MJ, Chan, ECS and Krieg, NR. 2001. Microbiology. 5th ed. Tata McGraw Hill 3. Sharma, PD. 2010.Microbiology. Rastogi Publications. 4. Singh, RP. 2015.Microbiology. Kalyani Publishers REFERENCE BOOKS: 1. Black, JG. 2012. Microbiology. 8th ed. John Wiley & Sons



2. Tortora, GJ. 2008. Microbiology. 9th ed. Pearson Education 3. Willey, J, Sherwood, L and Woolverton, C. 2011. Prescott's Microbiology. 8th ed. McGraw Hill

Education Course Learning Outcomes (CLO): On completion of this course, the students will be able to:

CLO Description Bloom’s Taxonomy Level

CLO1 Understand the differences between prokaryotic and eukaryotic cellular organization PLO1, PLO2


CLO2 Understand the differentiate between Gram +ve and Gram –ve bacteria by performing the experiment PLO1, PLO2, PLO3


CLO3 Explain the physiology and metabolism of bacterial growth PLO1, PLO2, PLO5

2 Understanding

CLO4 Learn the concepts of bacterial genetics and recombination and be

able to Analyze and apply it for genetic engineering and diverse industrial applications PLO1, PLO2,PLO3, PLO4,PLO5, PLO6, PLO12





Course

Learning Outcomes

Program Learning Outcomes (PLOs) Program Specific Outcomes(PSOs)

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CLO1 H M H L L M CLO2 H M M H M L M CLO3 H M L H L M M CLO4 M H H H M M M H M M H



Molecular Biology of the Cell MSB1008

L T P C 4 0 0 4

Course Objective: This course aims to give the student an overview of basic cell biology and its application. This course will focus on identifying key components that constitute living cells. The focus will be orientated around ‘Cell Biology at work’ with emphasise on key techniques currently used in the study of cells. UNIT I: Cell structure [8 Hours] Cell as a unit of life, prokaryotic, and eukaryotic cell, differences between plant and animal cell, general idea about lipid bilayer membranes, membrane transport of small molecules, cell adhesion, cell junction and extra cellular matrix, chemical composition of cell wall, cross linkage, porosity, tensile strength, turgor modifications in special types of cells, plasmadesmeta and fluid transport between cells. UNIT II: Cell organelles [8 Hours] Structure and functions of -Endoplasmic Reticulum: types – rough & smooth, intracellular transport & lipid biosynthesis, ribosomes, golgi apparatus, role of mitochondria in cellular energies & biogenesis, chloroplast, lysosomes: general organization, polymorphism, enzyme systems and their functions, vacuoles and ergastic substances, peroxisomes: formation, enzyme content and role. UNIT III: Nuclear material [8 Hours] Cytoskeleton: microtubules, microfilaments & associated proteins – actin, myosin and intermediate filaments, three dimensional organization of cytoskeleton, nucleus: nucleus, nuclear envelops, nucleoplasam, chromatin and chromosomes, nuclear division. UNIT IV: Cell division and cell cycle [8 Hours] Mitosis, meiosis and binary fission, cell cycle, cell cycle clock & check points, overview of cell cycle; molecular mechanisms for regulating mitotic events; check points in cell cycle regulation; meiosis; cell birth, lineage and death. UNIT V: Microbial cell biology [8 Hours] Structural organization of prokaryotic cell, cell appendages – cilia, pili, fimbriae & flagella, cell wall structure and bacterial surface layers, cytoplasm, bacteria as example for prokaryote, Eukaryotic cell organization – filamentous fungus and yeast as example. TEXT BOOKS: 4. Alberts, B., Bray,D. , Johnson, A. , Lewis, J. , Roff, M. , Robert, K., Walter, P. and Roberts, K.

2009. Essential Cell Biology: An Introduction to the Molecular Biology of the Cell. 3rd ed. Garland Publishing Company.

5. Trophe, N. 1984. Cell Biology, International Edition. John Wiley & Sons, REFERENCE BOOKS: 1. Lodish, H., Berk, A., Zipursky, S.L., Matsudaura, P., Baltimore, D. and Danell, J. 2007. Molecular

Cell Biology. 6th ed. W.H. Preeman and Company. 2. Karp, G. 2013. Cell and Molecular Biology. 7th ed. John Wiley and Sons Inc. 3. Robertis, D., Lea & Febiger. 1987. Cell and Molecular Biology, 8th sub ed. Philadelphia. 4. Cooper, M. 1995. The Cell Molecular Approach. 2nd ed. ASM Press.



Course Learning Outcomes (CLO): On completion of this course, the students will be able to:


CLO1 Understand the coordination of function of different cell organelles to develop a functional cellular structure PLO1, PLO2


CLO2 Understand the process of cell division and growth, with a special focus upon microbial cells. PLO1, PLO2, PLO3


CLO3 Explain the organization and structure of cellular control system PLO1, PLO2, PLO5

2 Understanding

CLO4 Learn the concepts of cycle cycle regulation and able to

Analyze and apply its diverse roles in cancer cell research PLO1, PLO2,PLO3, PLO4,PLO5, PLO6, PLO7, PLO12


Applying,Analyzing Creating


Course Learning

Outcomes


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CLO1 H M H M M L CLO2 H H L H L M L CLO3 H M L H M M L CLO4 H H M L M H L M H H M M




Molecular Genetics MSB1009

L T P C 4 0 0 4

Course Objective: The course aims to illustrate the basics of genetics and inheritance and provide the students an understanding of the molecular basis of genetics and Mendel's fundamental work on genetics. The specific objectives are:

1. To explain the concepts of Mendelian genetics and its exceptions 2. To make the student understand mutation, linkage and crossing over 3. To explain the modes of inheritance viz. chromosomal & extra chromosomal 4. To explain population genetics and application of Hardy-Weinberg principle

UNIT I: Introduction to Mendelian Genetics and its exceptions [8 Hours] Mendelian laws of inheritance- Law of segregation and law of independent assortment, monohybrid crosses, dihybrid, trihybrid ratios, probability and fitting results in Mendelian inheritance, Chi-square; epistasis; multiple alleles; incomplete dominance; lethals; human pedigrees; patterns of single gene inheritance-autosomal recessive, autosomal dominant; X-linked inheritance; complicating factors-incomplete penetrance, variable expression UNIT II: Cytogenetics and mutations [8 Hours] Molecular concept of the gene, transposons, oncogenes and proto-oncogenes; errors in cell division, nondisjunction; variations in chromosome structure-giant polytene chromosome; chromosomal deletion, duplications, inversions, translocations; variations in chromosome number- euploidy; aneuploidy; polyploidy-allopolyploidy, autoployploidy Types of mutations- spontaneous, induced, point, frameshift, back and suppressor mutations; phenotypic effects of mutations; molecular basis of mutation; radiation and chemically induced mutation; detection of mutations. UNIT III: Sex determination and extrachromosomal inheritance [8 Hours] Mechanisms of sex determination-identification of sex chromosomes, XX-XY, XX-XO, ZZ-ZW mechanism of sex determination; environmental factors and sex determination; sex differentiation-sex chromatin bodies; dosage compensation- Barr body; sex influenced dominance; sex limited gene expression; sex-linked inheritance-patterns of inheritance of sex linked genes; X-linked traits in humans; deleterious recessive sex linked genes in humans; extrachromosomal inheritance-mitochondrial and plastid inheritance; maternal effect. UNIT IV: Linkage, crossing over and chromosome mapping [8 Hours] Linkage; crossing over; cytological basis of crossing over; molecular mechanism of crossing over; chromosome mapping, two factor crosses; three factor crosses; interference; ordered tetrad data, unordered tetrad data; physical chromosome mapping with in-situ hybridization; Deletion mapping, somatic cell hybridization mapping; practical applications of chromosome mapping UNIT V: Population genetics [8 Hours] Genetic variation-allele frequencies, random mating; Hardy Weinberg method and its applications; Inbreeding-the inbreeding coefficient, calculating F from pedigrees, genotype frequencies under inbreeding; quantitative effects of inbreeding, outbreeding and assortive mating; changes in allele frequencies- mutation, migration, selection, selection dynamics, random genetic drift; effects of random drift; genetic equilibrium- balancing selection, mutation selection balance, mutation drift balance TEXT BOOKS: 1. P.K. Gupta, 2014-2015, Genetics, 4th Edition, Rastogi Publications. 2. E. J. Gardner, M.J. Simmons and D.P. Snustad, 1991, Principles of Genetics, 8th Edition, John



Wiley & SONS, INC. REFERENCE BOOKS: 1. W.S. Klug and M.R. Cummings, 1997, Concepts of Genetics, 5th Edition, Prentice Hall

International, INC. 2. M.W. Strickberger, 2013, Genetics, 3rd Edition, PHI Learning Private Limited. 3. D.J. Fairbanks and W.R. Andersen, 1999, Genetics, The continuity of Life, 1st Edition,

Brooks/Cole Publishing INC.



Course Learning

Outcomes


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CLO1 H H L H H L L CLO2 H H M L H M L L CLO3 H H M H M M L CLO4 H H H M H M L H M L M



CLO1 Understand the genetic basis of heredity, Mendelian and non-Mendelian modes of inheritance. PO1, PLO2, PLO3

1, 2 Remembering,

Understanding CLO2 Understand basics of cytogenetics, extra-chromosomal

inheritance, linkage and cytoplasmic inheritance PLO1, PLO2, PLO3, PLO6


CLO3 Explain the different types of sex determination system in different organisms PLO1, PLO2, PLO3


CLO4 Learn the concepts of gene and allele frequencies and able to analyze and apply the Hardy-Weinberg equilibrium for population genetics. PLO1, PLO2, PLO3, PLO4, PLO6, PLO7, PLO12





Analytical Techniques in Biotechnology MSB1010

L T P C 4 0 0 4

Course Objective: The purpose of this course is to provide an understanding of fundamental concepts and underlying principles in the instruments used in biotechnology. In addition, the course is expected to develop the analytical skill to enable them to interpret the data. UNIT I: Microscopy [8 Hours] Important concepts in microscopy: Resolution, contrast, magnification; principle and application of light microscopy: compound, phase contrast; dark field, fluorescence and confocal microscopy, principle and application of electron microscopy- scanning and transmission electron microscopy. UNIT II: Centrifugation [8 Hours] Sedimentation: Centripetal force, centrifugal force and sedimentation coefficient. Principle of centrifugation: RCF and RPM, types of rotors: fixed angle, swinging bucket and vertical rotors, types of centrifuge and their uses, ultracentrifuge and its applications: preparative and analytical UNIT III: Chromatography techniques [8 Hours] Principle and application of paper chromatography; thin layer chromatography; gel filtration chromatography; column chromatography- ion-exchange chromatography; affinity chromatography; gas-liquid chromatography; high performance liquid chromatography. UNIT IV: Electrophoresis and blotting techniques [8 Hours] Principle of electrophoresis; agarose gel electrophoresis; sodium dodecyl sulphate-polyacrylamide gel electrophoresis; first dimension and second dimension electrophoresis; isoelectric focusing; capillary electrophoresis; southern, northern and western blotting. UNIT V: Spectroscopic techniques [8 Hours] Electromagnetic radiation; principle of absorption of light; principle and application of UV-Visible spectroscopy and IR spectroscopy; Fourier transform infrared spectroscopy; Fluorescence spectroscopy; NMR; Atomic absorption spectroscopy; Mass spectroscopy; Raman spectroscopy. TEXT BOOKS: 1. Ghosal, S. and Srivastava, A. 2009. Fundamentals of Bioanalytical Techniques and

Instrumentation. 1st ed. PHI Learning. 2. Wilson, K. and Walker, J. 2000. Principles and Techniques of Practical Biochemistry. 5th ed.

Cambridge University Press. 3. Sawhney, S. and Singh, R. 2000. Introductory Practical Biochemistry. 1st ed. Narosa Publishing

House. REFERENCE BOOKS: 1. Holme, D. and Peck, H. 1998. Analytical Biochemistry. 3rd ed. Longman. 2. Freifelder, D. 1982. Physical Biochemistry: Application to Biochemistry and Molecular Biology.

2nd ed. W.H. Freeman & Company. 3. Plummer, M. and Plummer, D. 1988. Introduction to practical biochemistry. 1st ed. Tata McGraw-

Hill Education.





Course Learning

Outcomes


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CLO1 H M L H M L L CLO2 H M H M H M L M CLO3 H H M H M M L CLO4 H H M L M M H M M H



CLO1 Understand the theory and practice of bio-analytical techniques PLO1, PLO2, PLO3

2 Understanding

CLO2 Understanding the instrumentation used in Biotechnology PLO1, PLO2, PLO5, PLO6


CLO3 Familiarity with working principals, tools and techniques of analytical techniques PLO1, PLO2, PLO5

2 Understanding

CLO4 Analyze the limitations and creative use of techniques for solving of research problem PLO1, PLO2, PLO3, PLO4, PLO5, PLO6





Biochemistry and Analytical Techniques Lab MSB1504

L T P C 0 0 2 1

1. Preparation of solutions: 1) percentage solutions, 2) molar solutions, 3) normal solutions 2. Standardization of pH meter, preparation of buffers, emulsions. 3. Spectroscopy: determination of absorption maxima (λmax) of a given solution 4. Titration of weak acid-weak base 5. Quantitative estimation of carbohydrates 6. Distinguish reducing and non-reducing sugars 7. Quantitative estimation of proteins 8. Estimation of nucleic acids 9. Determination of Acid value of a given fatty acid





Course Learning

Outcomes


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CLO1 Understand the preparation of different concentration of solutions PLO1, PLO2

2 Understanding

CLO2 Understanding the preparation of biological buffers and identify its properties PLO1, PLO2


CLO3 Explain the basic principle of spectrophotometer used to analyze the concentration of unknown solution PLO1, PLO2, PLO5

2 Understanding

CLO4 Identify the unknown biomolecule by applying its properties PLO1, PLO2, PLO3, PLO5, PLO6

2, 3, 4 Understanding,

Applying, Analyzing



Cell and General Microbiology Lab MSB 1505

L T P C 0 0 2 1

1. To study the dimensions of a cell with the help of a micrometer. 2. To study the Mitosis in Onion Root Tips. 3. To perform cell count by using hemocytometer. 4. To prepare and study the different types of routine lab media used in Microbiology lab (Basal

Media, Enriched Media, Selective Media and Differential Media). 5. To perform serial dilution for isolation of microbes from soil sample and prepare glycerol stock for

preservation of microbes. 6. To perform screening of microbes grown on different media types and preparing their culture. 7. Counting colony forming units (CFU) and calculating the number of bacteria in the original

solution. 8. J MOL Via spoken tutorial: Microbial cellular proteins.





Course Learning Outcomes

Program Learning Outcomes (PLOs) Program Specific Outcomes

(PSOs)

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CLO3 H M L H L L M

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CLO1 Understand the different phase of cell division PLO1, PLO2

2 Understanding

CLO2 Understanding the preparation of different types of media used in microbial cultivation PLO1, PLO2


CLO3 Explain the basic knowledge of isolation procedure of microbes form different natural sources PLO1, PLO2, PLO3

2 Understanding

CLO4 Analyzing the number of bacteria in the original solution PLO1, PLO2, PLO3, PLO5, PLO6


Applying, Analyzing



Seminar / Journal Club-I MSB1507

L T P C 0 0 1 1

Course Learning Objectives: The objective of this course is 1. To acquire the skills necessary to read and evaluate original research articles. Most of the course

will involve the discussion of current issues in the domain of biotechnology. 2. To encourage the students to study advanced engineering developments 3. To prepare and present technical reports. 4. To encourage the students to use various teaching aids such as overhead projectors, power point

presentation and demonstrative models. Course Learning Outcomes (CLO): On completion of this course, the students will be able to

CLO Description Bloom’s Taxonomy Level CLO1 Survey the changes and updating of selected topic to know

the current research of particular area PLO2, PLO3, PLO4

4 Analyzing

CLO2 Analyze and compile the data of selected topic and interpret the impact on the society and environment PLO1, PLO2, PLO3

2, 5 Understanding,

Evaluating CLO3 Compile the report of the study and present to the audience

with following the ethics. PLO4, PLO5, PLO8

6 Creating

CLO4 Develop an understanding to review, and compile the data and also developed the presentation skills PLO2, PLO4, PLO6, PLO8, PLO10, PLO12

2, 6 Understanding,

Creating





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Molecular Biology MSB2005

Objectives: This course is to expose the students to the chromosome structure & gene expression in both prokaryotes and eukaryotes. It also familiarizes students with extra chromosomal elements, antisense technology. UNIT I: Structure of DNA and RNA [8 Hours] Introduction to nucleic acids: Nucleic acids as genetic material, Structure and physicochemical properties of elements in DNA and RNA, Biological significance of differences in DNA and RNA. Primary structure of DNA: Chemical and structural qualities of 3’, 5’-Phosphodiester bond. Secondary Structure of DNA: Watson & Crick model, Chargaff’s rule, Tertiary structure of DNA: DNA supercoiling. UNIT II: DNA replication and repair [8 Hours] Central Dogma of molecular biology, DNA Replication- Prokaryotic DNA Polymerase I, II and III, Eukaryotic DNA Polymerases, Fidelity and Catalytic Efficiency of DNA Polymerases, Okazaki Fragments, Replication Origin, Primosomes, Concurrent Replication Mechanism Involving Leading and Lagging Strands of DNA. DNA repair- enzymes; Photoreactivation; Nucleotide excision repair; Mismatch correction; SOS repair UNIT III: RNA transcription and processing [10 Hours] Transcription: Prokaryotic and Eukaryotic Transcription.-RNA polymerase sub units, different sigma factors, initiation, elongation and termination - rho dependent and independent; RNA processing enzymes, modification in RNA: 5’-Cap formation; Transcription termination; 3’-end processing and polyadenylation; Splicing; RNA Editing, Nuclear export of mRNA; mRNA stability. Different modes of mRNA, tRNA, and rRNA splicing, role of various snRNPs UNIT IV: Translation and posttranslational modification [6 Hours] Introduction to Genetic code: Elucidation of genetic code, Codon degeneracy, Wobble hypothesis and its importance, Prokaryotic and eukaryotic ribosomes. Steps in translation: Initiation, Elongation and termination of protein synthesis, inhibitors of protein synthesis, Post- translational modifications and its importance, UNIT V: Regulation of gene expression [8 Hours] Regulation of gene expression in prokaryotes-The operon concept, lac & trp operons, Transcriptional control, Post translational control, Regulation in eukaryotes - Control by promoter, enhancer and silencers. Cis-trans elements, DNA methylation, Antisense technology, RNAi, siRNA, microRNAs TEXT BOOKS: 1. Friefelder, David. “Molecular Biology.” Narosa Publications, 1999. 2. Weaver, Robert F. “Molecular Biology” 2nd Edition, Tata McGraw-Hill, 2003. 3. Karp, Gerald “Cell and Molecular Biology: Concepts and Experiments” 4th Edition, John Wiley,

2005. 4. Friefelder, David and George M. Malacinski “Essentials of Molecular Biology” 2nd Edition,

Panima Publishing, 1993. 5. Lewin’s GENES XI, Published by Jones & Bartlett Learning; 11 edition (January 15, 2013).

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REFERENCE BOOKS: 1. Tropp, Burton E. “Molecular Biology: Genes to Proteins”. 3rd Edition. Jones and Bartlett, 2008. 2. Glick, B.R. and J.J. Pasternak. “Molecular Biotechnology: Principles and Applications of

Recombinant DNA” 4th Edition. ASM, 2010. Course Learning Outcomes (CLO): On completion of this course, the students will be able to:


CLO1 Basic understanding of structure of RNA and DNA PLO1, PLO2

2 Understanding

CLO2 Remember and understand the process of DNA replication and repair mechanisms. PLO1, PLO2


CLO3 Understand the process of transcription, translation and post-translation. PLO1, PLO2

2 Understanding

CLO4 Learn to understand, analyze, and apply the gene

regulation mechanism in various scientific fields, and creating new techniques. PLO1, PLO2,PLO3, PLO4,PLO5, PLO6, PLO12






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Biology of Immune System MSB2006

Course Objective: Role of antibody engineering in biomedical applications and the importance of immunology in disease processes, tissue transplantation and immune regulation are some of the areas of attributes of this course which can help the students to understand the biotechnology related to human kind. UNIT I: Introduction to Immunology [10 Hours] Introduction to immunology- History and evolution of immune system; Innate and acquired immunity, Humoral and cell mediated immunity, Hematopoiesis, cells and organs of immune system; Concept of immunogenicity, antigens, epitopes, and haptens. UNIT II: Antibodies [10 Hours] Antibodies-structure, classes, functions, Molecular basis of antibody diversity: DNA rearrangements; variations arising out of V, D, J joining; somatic hypermutation, class switching; B and T cell generation, maturation, and their receptors, MHC: gene organization, types of MHC molecules and their structure, basis and significance of MHC polymorphism UNIT III: Antigen processing and presentation [6 Hours] Antigen processing and presentation; Cytokines and their role in immune response; Complement system-classical, alternate and lectin, Monoclonal antibodies, hybridoma technology and polyclonal antibodies. UNIT IV: Antigen antibody interactions [6 Hours] Antigen-antibody interactions- agglutination, immunodiffusion, immunoassay-ELISA (competitive, sandwich and indirect), RIA, Immunoelectrophoresis. UNIT V: Immunodeficiencies [8 Hours] Imunnodeficiencies: Primary and secondary immunodeficiencies, transplantation immunology: immunologic basis of graft rejection, immune tolerance to grafts; vaccine immunization and their types, Hypersensitivity and its types, Inflammatory response; Immune tolerance; Autoimmunity-organ specific and systemic. TEXT BOOKS: 1. Khan, Fahim Halim “Elements of Immunology” Pearson Education, 2009. 2. Sudha Gangal and Shubhangi Sontakke,Text of Basic and Clinical Immunology,FIRST,University

Press,2013. REFERENCE BOOKS: 1. Kuby Sharon Stranford, Judy Owen, Jenni,Kuby Immunology,5TH EDITION,MAC

Publications,1997

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CLO1 Understanding and remembering about immune system and its types. PLO1, PLO2


CLO2 Remember and understand the structure of antibody and its functions PLO1, PLO2


CLO3 Understand, apply, and create new technologies for the production of MABs and hybridoma technology in various applications. PLO1, PLO2, PLO3, PLO4,PLO5, PLO6, PLO7, PLO12


Applying, Creating

CLO4 Analyze, apply, and create immunological techniques for better understanding of immune disorders. PLO1, PLO2,PLO3, PLO4,PLO5, PLO6, PLO12






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Enzymology MSB2007

Course Objective: The course aims to provide a basic understanding of the enzyme their properties and application in various industries. UNIT I: General introduction of enzyme [8 Hours] Introduction and scope, Nomenclature, coenzyme and cofactors, Mechanisms of enzyme action, concept of active site and concept of ES complex, specificity of enzyme action, significance and evaluation of energy of activation, enzyme unit and turn over number. UNIT II: Enzyme kinetics [10 Hours] Order of reactions, kinetics of enzyme reaction–single and bi-substrate reaction, Michaelis-Menten equation, Different plots for the determination of Km & Vmax and their physiological significances, derivation of Michaelis-Menten equation, Allosteric enzyme, different type of enzyme inhibition, UNIT III: Enzyme immobilization [8 Hours] Physical and chemical techniques for enzyme immobilization, adsorption, matrix entrapment, encapsulation, cross-linking, covalent binding etc, Examples advantages and disadvantages of different immobilization techniques UNIT IV: Multi enzyme system [6 Hours] Mechanism of action and regulation of pyruvate dehydrogenase & fatty acid synthase complexes, Enzyme -enzyme interaction, multiple forms of enzymes with special reference to lactate dehydrogenase UNIT V: Application of enzymes [8 Hours] Commercial applications of enzymes in food, pharmaceutical and other industries, enzymes for analytical and diagnostic applications, purification and characterization of enzymes from natural sources, different methods of enzyme characterization TEXT BOOKS: 1. Principles of Biochemistry BY A. Lehninger (1987) 2. Trevor Palmer and Philip Bonner; Enzymes: Biochemistry Biotechnology, Clinical chemistry. REFERENCE BOOK: 1. Colin J. Suckling & Colin L. Gibson; Enzyme Chemistry: Impact & Application; Blackie Academic &Professional

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CLO Description Bloom’s Taxonomy Level CLO1 Basic Understanding and remembering about enzymes and

its kinetics. PLO1, PLO2


CLO2 Apply and create enzyme immobilization techniques. PLO1, PLO2

4, 6 Applying, Creating

CLO3 Understand the mechanism of enzyme action and its regulation. PLO1, PLO2, PLO3, PLO4

2 Understanding

CLO4 Analyze, apply, and create application of enzymes in different industries. PLO1, PLO2,PLO3, PLO4,PLO5, PLO6, PLO12

3, 4, 6 Applying, Analyzing

Creating Mapping of CLOs with PLOs & PSOs Course Learning Outcomes


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Bioinformatics MSB2008

Course Objective: 1. Understand the rapid use of bioinformatics. 2. To deeply learn the bioinformatics tools those are used in various field of Biotechnology. UNIT I: Introduction to biological database [10 Hours] Overview, Types of biological databases, Nucleic acid databases (NCBI, EMBL, DDBJ etc); protein databases; Genbank, PRINT, BLOCK, P-fam, Prosite; applications of bioinformatics in biotechnology: gene prediction in prokaryotes, eukaryotes; other applications in the areas of health, food and medicine. UNIT II: Sequence analysis and alignment [8 Hours] Statistical significance of alignment; Sequence assembly Analysis; Global & Local Alignment and their algorithms, Pair-wise and multiple sequence alignment: goals and methods for sequence alignment. Dot plots, Dynamic programming algorithms, Heuristics- FASTA, BLAST; Scoring matrices- PAM, BLOSUM, HMM etc., Gaps & gap penalties. UNIT III: Phylogenetic analysis [10 Hours] Elements of phylogenetic models, phylogenetic data analysis: alignment, substitution, parsimony, model building, building the data model (alignment), determining substitution model, phylogenetic prediction, evolutionary tree construction (rooted and unrooted tree), tree building methods (distance-based and character-based method), evaluation tree & data phylogenetic software like PHYLIP, CLUSTAL W.

UNIT IV: Protein structure prediction [6 Hours] Protein structure classification (primary, secondary, tertiary and quarternary), domains, folds, motifs; 3D proteins structure file formats: PDB, MMDB; secondary structure prediction methods (Chou-Fasman and GOR method) & tertiary structure predictions (threading, Homology modeling); Different Protein visualization tools: Rasmol, Swiss-PDB etc. UNIT V: Drug discovery and development [6 Hours] Drug and targets, steps involved insilico drug designing: target identification, target validation, lead compounds, lead optimization, clinical trials; computer aided drug design (CADD): structure based and ligand based; molecular docking and QSAR. TEXT BOOKS: 1. Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins, By Andreas D, Baxevanis,

B.F, Francis O, Wiley –Interscience , 2nd Edition, 2001. 2. Sequence and genome analysis dy D.W.Mount-Cold Spring Harbour Lab., 1st edition, 2004. REFERENCE BOOK: 1. Bioinformatics: A Biological Guide to computing and the Internet by Stuart M and Brown NYU,

Mecical Centre, NY USA, 2000. 2. Bioinformatics and the genome projects by Smith DW, Academic Press, 1993.

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Level CLO1 Understanding and remembering about biological

databases and its application in various sectors. PLO1, PLO2,PLO6, PLO7


CLO2 Remembering, understanding and creating sequence alignment by applying appropriate algorithms. PLO1, PLO2, PLO3, PLO4

1, 2,4,6 Remembering, Understanding,

Applying, Creating CLO3 Creating phylogenetic trees by applying and evaluating

suitable methods. PLO1, PLO2, PLO3, PLO4

4,5,6 Applying,

Evaluating, Creating CLO4 Analyze, apply, and create protein structure and perform

drug designing. PLO2,PLO3, PLO4,PLO5, PLO6, PLO12

2, 3, 4, 6 Applying, Analyzing

Creating Mapping of CLOs with PLOs & PSOs Course Learning Outcomes


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Biostatistics MMA2007

Course Objective: 1. To introduce some fundamental ideas about Moments, Skewness and kurtosis by moments. 2. To provide understanding of the basic concepts of Probability and Probability Distribution. 3. To aim at understanding of Statistical Quality Control, Correlation and regression analysis,

Testing of Hypothesis and Analysis of variance. 4. To explore the connection between basics as well the advance tools of the subject to demonstrate

the link between theory and its real world applications. UNIT-I: Measures of central tendency, Dispersion [8 Hours] Moments, Moments about Mean, Moments about any number and origin, Relation between Moments about Mean and Moments about any number & origin, measures of Skewness and kurtosis by moments. UNIT- II: Probability and Probability Distribution [10 Hours] Probability: Additive and multiplicative rule, Permutation and combination, compound probability, Bayes theorem. Elementary ideas of Binomial, Poisson and Normal Distribution-Assumption, mean and standard deviation for all distribution. UNIT-III: Statistical Quality Control [6 Hours] Introduction, Ways to control quality of a product, Causes of Variation, Advantages, Types, Control Chart, Types of control charts, Use of control chart. UNIT-IV: Correlation and regression analysis [8 Hours] Correlation: Significance, Types, properties, computation methods: Karl’s Pearson and spearman method, correlation coefficient, Regression: Types, Assumptions, computation and regression coefficient. UNIT-V: Testing of Hypothesis and Analysis of variance [8 Hours] Degree of freedom, Hypothesis, Level of significance, Type–I, Type-II Error, Test of significance

based on t, -test and ANOVA (one way classification). TEXT BOOKS: 1. S.C. Gupta & V. K. Kapur, Probability & Statistics, S. Chand & Sons Publication. 2. T2. A. M. Goon, M. K. Gupta & B. Das Gupta, Fundamental of statistics, world press Calcutta. REFERENCE BOOK: 1. Gupta, C. B. 1995. An Introduction to Statistical Methods, 9th ed., Vikas Publication. 2. R2. Das, N. G. 2009. Statistical Methods-Volume 1 and 2. 1st ed, Tata McGraw-Hill. 3. R3. Spiegel, M. R. and Stephens, L. J. 2000. Statistics. 3rd ed. Tata McGraw-Hill.

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CLO1 Basic understanding of Moments, Skewness and kurtosis by moments. PLO1, PLO2, PLO3, PLO4

2 Understanding

CLO2 Remember and understand the Probability and Probability Distribution. PLO1, PLO2, PLO3, PLO4


CLO3 Understand the Statistical Quality Control, Correlation and regression analysis. PLO1, PLO2, PLO3, PLO4

2 Understanding

CLO4

Learn to understand, analyze, and apply The Testing of Hypothesis and Analysis of variance. PLO1, PLO2, PLO3, PLO4






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Molecular Biology and Bioinformatics Lab MSB2506

1. Preparation of plasmid DNA by alkaline lysis method

2. Genomic DNA isolation from bacteria

3. Isolation of DNA from plants by Hexadecyl trimethyl-ammonium bromide (CTAB) method

4. RNA isolation from plant tissues

5. Multiple sequence alignment using Clustal Omega

6. Identification of conserved domain(s) present in a protein

7. Prediction of protein structure

8. Identification of putative promoter sequences in a given gene Course Learning Outcomes (CLO): On completion of this course, the students will be able to:


CLO1 Understanding mechanisms for isolation of DNA from different sources PLO1, PLO2

2 Understanding

CLO2 Understanding the basis of visualization of nucleic acid by electrophoresis. PLO1, PLO2

1, 2 Remembering, Understanding,

CLO3 Understanding the importance of conserved domain for evolutionary identification PLO1, PLO2

1, 2 Remembering, Understanding,

CLO4 Analyze the protein structure and sequences of its gene to modify its function according to the need PLO1, PLO2

4 Analyzing

Mapping of CLOs with PLOs & PSOs Course Learning Outcomes


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Immune System and Enzymology Lab MSB2507

1. Enzyme assay; activity and specific activity determination of amylase. 2. Enzyme Kinetics: Effect of varying substrate concentration on enzyme activity, determination of

Michaelis-Menten constant (Km) and Maximum Velocity (Vmax.) using Lineweaver-Burk plot. 3. Effect of temperature/pH and on enzyme activity.

4. Immobilization of enzyme.

5. Identification of blood groups.

6. To determine the Rh factor of the given blood sample.

7. Double diffusion techniques for identification of antigen-antibody samples (Immunodiffusion test). Course Learning Outcomes (CLO): On completion of this course, the students will be able to:


Course

Learning Outcom

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CLO1 Understanding about the mechanism of calculation of enzyme activity and specific activity to know the degree of percentage of protein purification PLO1, PLO2

2 Understanding

CLO2 Analyzed the kinetic parameter of enzyme and compare it with immobilized enzyme PLO1, PLO2

4 Analyzing

CLO3 Understand the mechanism for identification of blood group

PLO1, PLO2 2

Understanding

CLO4 Understand the mechanism of antigen and antibody reaction and apply it for identification of unknown antigen/antibody. PLO1, PLO2

2, 3 Understanding

Applying



Term Paper-I MSB2508

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Course Learning Objectives: The objective of this course is 1. To acquire the skills necessary to read and evaluate original research articles. Most of the course

will involve the discussion of current issues in the domain of biotechnology. 2. To encourage the students to study advanced engineering developments 3. To prepare and present technical reports. 4. To encourage the students to use various teaching aids such as overhead projectors, power point

presentation and demonstrative models. Course Learning Outcomes (CLO): On completion of this course, the students will be able to

CLO Description Bloom’s Taxonomy Level CLO1 Survey the changes and updating of selected topic to know

the current research of particular area PLO2, PLO3, PLO4

4 Analyzing

CLO2 Analyze and compile the data of selected topic and interpret the impact on the society and environment PLO1, PLO2, PLO3

2, 5 Understanding,

Evaluating CLO3 Compile the report of the study and present to the audience

with following the ethics. PLO4, PLO5, PLO8

6 Creating

CLO4 Develop an understanding to review, and compile the data and also developed the presentation skills PLO2, PLO4, PLO6, PLO8, PLO10, PLO12

2, 6 Understanding,

Creating





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Genetic Engineering MSB3007

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Course Objective: 1. To gain knowledge on gene manipulation using genetic engineering methods and its importance in

plant, animal and environmental biotechnology. 2. To understand the principle behind different enzymes and vectors used in recombinant DNA

technology. UNIT I: Introduction to genetic engineering [8 Hours] Milestones in genetic engineering and biotechnology, Restriction modification systems: Types I, II and III, mode of action, nomenclature, applications of Type II restriction enzymes in genetic engineering. DNA modifying enzymes and their applications: DNA polymerases, Terminal deoxynucleotidyltransferase, kinases, phosphatases, and DNA ligases. UNIT II: Vectors and cloning [8 Hours] Vectors: Definition and Properties, Cloning and Expression vectors, E. coli vectors; Properties of a good host, Plasmids. Plasmid vectors: Selection of recombinant DNA, pSC, pBR322 and pUC18 and pUC19, Bacteriophage vectors; lambda and M13 vectors, Cosmids, Artificial chromosome vectors: BACs, Yeast Vectors: YACs. UNIT III: Construction of cDNA and genomic library [8 Hours] cDNA library: Isolation of mRNA, preparation of cDNA, cloning of cDNA, problems in cDNA preparation, properties of cDNA and cDNA libraries, subtracted cDNA library (subtracted cloning), applications of cDNA library. Genomic library: construction of genomic library, amplified genomic library, subgenomic library, identification of the desired clone. UNIT IV: DNA amplification and DNA sequencing [8 Hours] Primer design; Fidelity of thermostable enzymes; DNA polymerases; Types of PCR – multiplex, nested, reverse transcriptase, real time PCR, touchdown PCR, hot start PCR, colony PCR. Sequencing methods; Enzymatic DNA sequencing; Chemical sequencing of DNA; Automated DNA sequencing; RNA sequencing; Chemical Synthesis of oligonucleotides UNIT V: Transfer and screening of recombinant [8 Hours] Techniques of gene transfer - transformation, transfection, micro injection, electroporation, lipofection and biolistic. Selection, screening and analysis of recombinants, principle of hybridization; Northern blotting, Southern blotting, Western blotting, fluorescent in-situ hybridization TEXTBOOKS: 1. Brown TA. (2010). Gene Cloning and DNA Analysis. 6th edition. Blackwell Publishing, Oxford,

U.K. 2. Mitra, S. 2001. Genetic Engineering: Principles and Practice, India, Macmillan India Limited 3. Primrose, S.B., Twyman, R.M. 2006. Principles of Gene Manipulation and Genomics, 7th edition.

Blackwell Publishing, Oxford, U.K. REFERENCE BOOKS: 1. Clark DP and Pazdernik NJ. (2009). Biotechnology: Applying the Genetic Revolution. Elsevier

Academic Press, USA 2. Sambrook J and Russell D. (2001). Molecular Cloning-A Laboratory Manual. 3rd edition. Cold

Spring Harbor Laboratory Press



3. Brown, T. A. 2007. Genomes-3. Garland Science Publishers. Course Learning Outcomes (CLO): On completion of this course, the students will be able to:


CLO1 Understand about the use of enzymes in genetic engineering PLO1, PLO2, PLO3


CLO2 Explain gene cloning, transformation and transfection and techniques used in genetic engineering PLO1, PLO2


CLO3 Explain genomic and cDNA library construction for cloning and scopes and applications of genetic engineering PLO1, PLO2, PLO3

2 Understanding

CLO4 Apply theoretical knowledge of genetic engineering for development of new recombinant DNA molecules PLO1, PLO2, PLO3, PLO6, PLO7, PLO12





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Genomics and Proteomics MSB3008

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Course Objective: The broad objective of the course is to make students aware about the impact of Genomics and Proteomics technologies on understanding genome and proteome complexities. The specific objectives are as follows: 1. To introduce the concepts of Genomics and Proteomics. 2. To provide knowledge of applications of Genomics and Proteomics. UNIT I: Introduction to Genomics: [8 Hours] Introduction to genomics; organization of eukaryotic versus prokaryotic genomes; genome diversity and features of E. coli, Saccharomyces cerevisiae, Caenorhabditis elegans, Mus musculus, Homo sapiens, Arabidopsis thaliana genomes; genome projects: Human Genome Project, HapMap project, 1000 genome project and ENCODE project. UNIT II: Genomics methods [8 Hours] Current trends in whole genome sequencing (NovaSeq-Illumina, PacBio-Sequel); whole genome shotgun sequencing; DNA sequence contigs; assembly of a contiguous DNA sequence: clone contig approach; DNA sequence assembler (Newbler and VarScan,); transcriptome analysis by DNA microarrays, tissue microarrays, SNP arrays; RNA-seq UNIT III: Comparative Genomics and Pharmacogenomics [8 Hours] Comparative genomics: phylogeny cluster of orthologous genes (COGS); Paralogous genes and gene displacement; horizontal gene transfer; comparative genomics of bacteria, organelles and eukaryotes; pharmacogenomics and personalized medicine. UNIT IV: Introduction to Proteomics and methods [8 Hours] Introduction and types of proteomics; two-dimensional polyacrylamide gel electrophoresis (2D-PAGE); mass-spectrometry: Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-ToF); liquid chromatography coupled with mass spectrometry (LC-MS); peptide mass fingerprinting; protein arrays; yeast two hybrid system; phage display UNIT V: Applications of Proteomics [8 Hours] Protein-protein interactions in health and disease; applications of proteomics in drug development and toxicology; glycobiology and proteomics; proteomics as a tool for plant genetics and breeding TEXT BOOKS 1. Primrose SB and Twyman RM (2006). Principles of Gene Manipulation and Genomics, 7th ed.

Blackwell Publishing 2. Pennington SR and Dunn MJ (2002). Proteomics: From Protein Sequence to Function 1st ed. Viva

Books Private Limited 3. Liebler DC and Yates III JR (2002). Introduction to Proteomics: Tools for new biology, 1st ed.

Humana Press



REFERENCE BOOKS 1. Westermeier R and Naven T (2002). Proteomics in Practice: A Laboratory Manual of Proteome

Analysis. 1st ed. Wiley-VCH Verlag-GmbH 2. LESK AM (2014). Introduction to Bioinformatics.4th ed. Oxford University Press Course Learning Outcomes (CLO): On completion of this course, the students will be able to:


CLO1 Understand the genome diversity in different organisms and the importance of genomics PLO1, PLO2, PLO3, PLO6


CLO2 Understand different applications of comparative genomics and pharmacogenomics PLO1, PLO2, PLO3


CLO3 Explain various methods to study dynamic proteome PLO1, PLO2

2 Understanding

CLO4 Apply different proteomics tools for drug development and toxicological studies PLO1, PLO2, PLO3, PLO5, PLO6, PLO12




Course

Learning Outcomes


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Bioprocess Engineering and Technology MSB3009

L T P C 3 0 0 3

Course Objective: This course is designed for familiarizing students with basic idea of microbial kinetics, upstream processing, bioreactor design and operation, and downstream processing. At the end of the course, the students would have learnt about fermentation processes, kinetics of microbial growth and all the steps involved upstream and downstream processing for any production process. The course also helps to understand the industrial applications of bioprocess engineering. UNIT I: Introduction to Bioprocess technology and basic mode of fermentation [8 Hours] Isolation, screening and maintenance of industrially important microbes; Outline of an integrated bioprocess: upstream and downstream, unit operations involved in bioprocesses, generalized process flow sheets; Concepts of basic modes of fermentation - batch, fed batch and continuous; Microbial growth and death kinetics. UNIT II: Principles of upstream processing [8 Hours] Medium formulation: Carbon sources, Nitrogen sources, Minerals, Chelators, Growth factors, Antifoams, Introduction to media optimization; Inoculum development: criteria for the transfer of inoculum, development of inocula for bacterial processes; Sterilization: batch and continuous heat sterilization of liquid media, filter sterilization of liquid media. UNIT III: Design of novel bioreactors [8 Hours] Introduction to ideal and non-ideal bioreactors; Design and components of various bioreactors: Stirred tank (CSTR), packed bed bioreactors, Bubble-column bioreactors, fluidized bed bioreactors, trickle bed bioreactors, airlift loop bioreactors, photo bioreactors. Methods of measuring process variables, online and offline analytical methods. UNIT IV: Introduction to downstream processing [8 Hours] Bioseparation processes – filtration (conventional and microfilteration), centrifugation (settling of solids and centrifugal filteration), cell disruption (Chemical and mechanical); Isolation Processes: batch extraction and adsorption; Product purification: precipitation with salt and nonsolvent, ultrafilteration and chromatographic techniques; Polishing: crystallization, drying, storage and packaging. UNIT V: Application of bioprocess engineering in industries [8 Hours] Fermented foods and beverages (Baker yeast production), Environmental industry (Biological waste water treatment), medical applications of bioprocess engineering (Tissue engineering, Gene therapy). TEXT BOOKS: 1. Stanbury, P.F., Hall S. J. and Whitaker A. 2003. Principles of Fermentation Technology. 2nd ed.

Science & Technology Books. 2. Doran P. 2012. Bioprocess Engineering Pinciples, 2nd ed. Academic Press 3. Nielsen, J. and Villadsen, J. 2007. Bioreaction Engineering Principles. 2nd ed. Springer science and

business media. REFERENCE BOOKS: 1. Bailey J. E. and Ollis D. F. 1986. Biochemical Engineering Fundamentals, 2nd ed., McGraw Hill. 2. Shuler M. L. and Kargi F. 2002. Bioprocess Engineering: Basic Concepts. 2nd ed. Prentice Hall Inc.





CLO1 Understand biological and kinetic concepts underlying bioprocesses engineering PLO1, PLO2, PLO6

2 Understanding

CLO2 Explain procedures for the design and control of bioreactors PLO1, PLO2, PLO3

2 Understanding

CLO3 Understand the basic upstream processing principles PLO1, PLO2

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CLO4 Apply the bioprocess engineering in different industries for the

benefit of mankind PLO1, PLO2, PLO3, PLO6, PLO12





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IPR, Bioethics and Biosafety MSB3010

L T P C 3 0 0 3

Course Objective: This course serves as a primer for intellectual property rights, patenting, ethical issues and Biosafety in context to biotechnology. The specific objectives of the course are: 1. To teach students about intellectual property rights (IPRs) and their different kinds 2. To make students understand the process of patent filing 3. To make students aware about the patenting of materials of biological origin 4. To impart knowledge of ethical practices appropriate to biotechnology research UNIT I: Intellectual Property Rights [7 Hours] Introduction to intellectual property rights; requirements and utility of IPRs; different types of IPRs; features of World Intellectual Property Organization (WIPO); TRIPS agreement; international treaties and conventions on intellectual property. UNIT II: Patents [6 Hours] Fundamentals of patent; conditions for the grant of patents; what can be and what cannot be patented; types of patents; patenting agencies; filing patents in India; procedure for grant of patents; patenting of biological material; patenting of transgenic, isolated genes and DNA sequences. UNIT III: Copyright, trademark and geographic indication [6 Hours] Introduction to copyright and its applicability; copyright registration in India; laws and policies regarding copyright (Berne convention and Copyright (Amendment) Act, 2012); fundamental concepts and importance of trademark; relevance of geographical indication. UNIT IV: Bioethics [7 Hours] Need of bioethics; definitions of bioethics; applications of bioethics and its relations with other branches of studies; ethical issues in genetically modified organisms; bioethical implications of human genome project; ethical issues in stem cell research and use; ethical issues in biodiversity management; case study on ethical issues surrounding vaccines in food. UNIT V: Biosafety [7 Hours] Introduction to biosafety; biosafety guidelines in India; biosafety levels; Cartagena Protocol on Biosafety; implementation of biosafety guidelines; institutional biosafety committee; biosafety implications in GMO research. TEXT BOOKS: 1. Pandey, N and Dharni, K. 2014. Intellectual Property Rights. Prentice-Hall of India Pvt.Ltd 2. Sateesh, MK. 2008. Bioethics and Biosafety. I K International Publishing House Pvt. Ltd REFERENCE BOOKS: 1. Ganguli, P. 2001. Intellectual Property Rights: Unleashing the Knowledge Economy. Tata

McGraw-Hill Publishing Company 2. Thomas, J and Fuchs, R. 2002. Biotechnology and Safety Assessment. 3rd ed. Academic Press





CLO1 Understand to differentiate between diverse types of IPRs PLO1, PLO2, PLO8

2 Understanding

CLO2 Understand the different types of patents, and learn about the importance of patent filing PLO1, PLO2

2 Understanding

CLO3 Understand the various ethical issues of biotechnology PLO1, PLO8

2 Understanding

CLO4 Learn about policies, guidelines and laws governing ethical practices

in biotechnology research PLO1, PLO8, PLO10


Applying, Analyzing


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Technical Writing and Literature Survey MSB3011

L T P C 2 0 0 2

Course Objective: This course is designed to provide the fundamental knowledge of writing a document. Preparation of manuscripts for publications in the form of Research Paper, Review Articles. While doing so students are expected to perform the Review of Literature, Compilation and searching of the primary and secondary data.

UNIT I: Technical Writing [8 Hours] Introduction of Technical writing, types of documents, Importance of Technical writing. Role of Teacher and researcher in Technical writing. Documentation process, evolution of technical writing. Collection of literature and compilation of a document.

UNIT II: Technical Writing Software [8 Hours] Processing of the Text collected with the help of various softwares like- MS-word, Text processing. Properties of a good text document. Checking the documents for proper usage of language and spellings.

UNIT III: Literature Survey [8 Hours] Introduction of Literature Review, steps of Reviewing, Collection and storage of Review searched. Citation of searched Literature. Keeping your reference ready for publication with the help of Googlescholar or any other tools.

TEXT BOOK: 1. Basu B. N. (2007) Technical Writing PHI Publication Pvt. Ltd

Course Learning Outcomes (CLO): On completion of this course, the students will be able to: CLO Description Bloom’s Taxonomy Level

CLO1 Understand to collect the scientific literature on a particular subject PLO1, PLO4,

2 Understanding

CLO2 Understand the various tools such as Google scholar etc. used in literature search PLO1, PLO2

2 Understanding

CLO3 Learn to compile the research date PLO1, PLO10

2, 3 Understanding, Apply

CLO4 design the scientific project and reports PLO1, PLO2, PLO9, PLO10, PLO12

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Nanobiotechnology MSB3107

L T P C 4 0 0 4

Course Objective: The aim of this course is to provide basic knowledge in the interface between chemistry, physics and biology on the nanostructure level with a focus on biotechnological usage. UNIT I: Introduction to Nanobiotechnology [8 Hours] Nanotechnology definition and concepts; Cellular Nanostructures; Nanopores; Bimolecular motors; Criteria for suitability of nanostructures for biological applications UNIT II: Basic characterization techniques [8 Hours] Electron microscopy; Atomic force microscopy; Photon correlation spectroscopy. UNIT III: Nano structures [8 Hours] Thin films; Colloidal nanostructures Nano vesicles; Nano spheres; Nano capsules. UNIT IV: Nanostructures for drug delivery [8 Hours] Nanostructures for drug delivery, concepts, targeting, routes of delivery and advantages. UNIT V: Applications of nano structures [8 Hours] Nanostructures for diagnostics and biosensors; Nanoparticles for diagnostics and imaging; Nano devices for sensor development. TEXTS BOOKS: 1. Multilayer Thin Films, Editor(s): Gero Decher, Joseph B. Schlenoff Publisher: Wiley-VCH Verlag

GmbH & Co. KGaA ISBN: 3527304401 2. Bionanotechnology: Lessons from Nature Author: David S. Goodsell Publisher: Wiley-Liss ISBN:

047141719X REFERENCE BOOKS: 1. Biomedical Nanotechnology Editor: Neelina H. Malsch Publisher: CRC Press ISBN: 0-8247-2579-

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Level CLO1 Learn the foundational of the Nano science and related fields.

PLO1, PLO2, PLO3 2

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CLO2 Understand the application and impact of nanomaterials on environment PLO1, PLO2, PLO3

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CLO3 Apply their learned knowledge to develop Nanomaterial’s. PLO1, PLO2, PLO3

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Applying CLO4 Apply the nanotechnology for advancing medical science

thereby improving health care practices PLO1, PLO2, PLO3, PLO6, PLO12

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Environmental Biotechnology MSB3108

L T P C 4 0 0 4

Course Objectives: The objectives of this syllabus are to help the students to acquire awareness and sensitivity towards the environment. Understand the issues, questions and problems related to environment and its development. The use of Biotechnology and its tool in finding solution to the pollution and environment related issues. UNIT I: Reconceptualization of Environmental Biotechnology [8 Hours] Environment, segments of the environment; Principles and cyclic pathways in the environment, Chemical composition of air, water, Radioactive and land pollutants; Sources; Sinks; Classification and effects of pollutants on living and nonliving things. UNIT II: Environmental monitoring and sample analysis [8 Hours] Sampling of air and water pollutants; Monitoring techniques and methodology, pH, Dissolved Oxygen (DO); Chemical oxygen demand (COD); Biological Oxygen Demand (BOD); Speculation of metals, monitoring & analysis of CO, NO2, CO2, SO2; Pesticide residue; Phenols and petrochemicals. UNIT III: Environmental Quality Assessment and Monitoring [8 Hours] What is environmental quality? Quality of environment for life on earth and man; Deterioration of environmental quality with reference to anthropogenic impact; Methods of assessment of environmental quality; Short term studies/surveys; Rapid assessment; Continuous short and long-term moni

m.sc. biotechnology programme 2019-20 and syllabus m. sc... · 2021. 1. 7. · m. sc. biotechnology...

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