mt-605 - moravek, incproducts.moravek.com/datasheets/mt605ds.pdfmt-605 riboflavin, [3h]- lot...
TRANSCRIPT
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MT-605
Riboflavin, [3H]-
Lot 213-021-0212-A-20090119-JG
A) All chromatograms were run using the HPLC method described on the Product Data Sheet.
Concentrations and volumes:
Standard solution concentration was 0.5 mg/ml.
Riboflavin, [3H]- concentration was 0.2 mCi/ml. Volume of standard alone injection was 2.0 µl.
Volume of Riboflavin, [3H]- alone injection was 1.0 µl.
Co-injection solution consisted of 1.5 µl Riboflavin, [3H]- + 2.0 µl standard.
Volume of co-injection was 3.5 µl.
Volume of blank injection was 1.0 µl.
B) Mass spectrometry – Positive mode C) NMR
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MT-605Riboflavin, [3H]-
Lot 213-021-0212-A-20090119-JG
Chromatogram Info:
File Name : L:\int21138 File Created : 2/25/2014 10:51:06 AM
Origin : Acquired, Acquisition started 5/17/2011 3:05:10 PM Acquired Date : 5/17/2011 3:25:10 PM
Project : Test By : Administrator
Method : unit2-20minrun By : Administrator
Description : Radiochemical trace of 3H material alone
Created : 8/8/2007 9:12 AM Modified : 2/25/2014 11:03 AM
Column : Detection : Radiochemical
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
5 10 15 20
[mV]
Volta
ge
0
100
200
300
400
500
3.62
1
4.26
2
4.73
3
5.42
4
7.92
5 L:\ int 21138 - Unit 2 Radio
2/25/2014 11:04 AM Page 1 of 2Chromatogram L:\int21138.PRM
-
Result Table (Uncal - L:\int21138 - Unit 2 Radio)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
Eff/l[t.p./m]
Symmetry/Tailing[-]
Response Factor
Resolution[-]
1 3.62 39.296 2.49 0.31 0.53 995.86 19917.25 1.15
2 4.26 69.916 4.31 0.55 0.92 1900.52 38010.47 0.78 1.5
3 4.73 168.914 8.24 1.33 1.77 2343.02 46860.44 1.52 1.2
4 5.42 35.916 1.48 0.28 0.32 1188.79 23775.79 3.08 1.4
5 7.92 12364.257 449.73 97.52 96.46 2406.54 48130.78 1.55 3.9
Total 12678.298 466.24 100.00 100.00
2/25/2014 11:04 AM Page 2 of 2Chromatogram L:\int21138.PRM
-
MT-605Riboflavin, [3H]-
Lot 213-021-0212-A-20090119-JG
Chromatogram Info:
File Name : L:\int21138 File Created : 2/25/2014 10:51:06 AM
Origin : Acquired, Acquisition started 5/17/2011 3:05:10 PM Acquired Date : 5/17/2011 3:25:10 PM
Project : Test By : Administrator
Method : unit2-20minrun By : Administrator
Description : UV trace of 3H material alone
Created : 8/8/2007 9:12 AM Modified : 2/25/2014 11:02 AM
Column : Detection : UV 266nm
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
5 10 15 20
[mV]
Volta
ge
-2
0
2
4
6
8
7.72
1
L:\ int 21138 - Unit 2 UV
2/25/2014 11:02 AM Page 1 of 2Chromatogram L:\int21138.PRM
-
Result Table (Uncal - L:\int21138 - Unit 2 UV)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
Eff/l[t.p./m]
Symmetry/Tailing[-]
Response Factor
Resolution[-]
1 7.72 26.518 1.98 100.00 100.00 8833.46 176669.22 0.87
Total 26.518 1.98 100.00 100.00
2/25/2014 11:02 AM Page 2 of 2Chromatogram L:\int21138.PRM
-
MT-605Riboflavin, [3H]-
Lot 213-021-0212-A-20090119-JG
Chromatogram Info:
File Name : L:\int21139 File Created : 2/25/2014 10:51:06 AM
Origin : Acquired, Acquisition started 5/17/2011 3:44:42 PM Acquired Date : 5/17/2011 4:04:42 PM
Project : Test By : Administrator
Method : unit2-20minrun By : Administrator
Description : UV trace of standard material alone
Created : 8/8/2007 9:12 AM Modified : 2/25/2014 11:05 AM
Column : Detection : UV 266nm
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
5 10 15 20
[mV]
Volta
ge
0
100
200
300
400
4.50
1
5.22
2
7.70
3
8.44
4
9.32
5
9.82
6
L:\ int 21139 - Unit 2 UV
2/25/2014 11:05 AM Page 1 of 2Chromatogram L:\int21139.PRM
-
Result Table (Uncal - L:\int21139 - Unit 2 UV)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
Eff/l[t.p./m]
Symmetry/Tailing[-]
Response Factor
Resolution[-]
1 4.50 19.845 2.42 0.42 0.66 7779.09 155581.75 0.82
2 5.22 18.157 1.23 0.39 0.34 7711.68 154233.66 1.03 3.3
3 7.70 4623.817 359.77 98.90 98.71 9418.50 188369.93 1.34 8.9
4 8.44 4.427 0.36 0.09 0.10 9865.85 197317.07 1.16 2.3
5 9.32 3.780 0.30 0.08 0.08 11639.23 232784.70 0.99 2.6
6 9.82 5.050 0.40 0.11 0.11 12516.61 250332.16 1.01 1.4
Total 4675.075 364.48 100.00 100.00
2/25/2014 11:05 AM Page 2 of 2Chromatogram L:\int21139.PRM
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MT-605Riboflavin, [3H]-
Lot 213-021-0212-A-20090119-JG
Chromatogram Info:
File Name : L:\int21140 File Created : 2/25/2014 10:51:06 AM
Origin : Acquired, Acquisition started 5/18/2011 8:52:45 AM Acquired Date : 5/18/2011 9:12:45 AM
Project : Test By : Administrator
Method : unit2-20minrun By : Administrator
Description : Radiochemical trace of 3H material co-injected with standard
Created : 8/8/2007 9:12 AM Modified : 2/25/2014 11:06 AM
Column : Detection : Radiochemical
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
5 10 15 20
[mV]
Volta
ge
0
100
200
300
400
500
600
700
3.53
1
4.27
2
4.74
3
7.83
4
L:\ int 21140 - Unit 2 Radio
2/25/2014 11:20 AM Page 1 of 2Chromatogram L:\int21140.PRM
-
Result Table (Uncal - L:\int21140 - Unit 2 Radio)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
Eff/l[t.p./m]
Symmetry/Tailing[-]
Response Factor
Resolution[-]
1 3.53 87.540 3.05 0.46 0.44 214.58 4291.54 1.34
2 4.27 123.887 7.10 0.65 1.04 948.06 18961.11 0.87 1.0
3 4.74 302.210 11.28 1.58 1.65 1323.53 26470.57 2.21 0.9
4 7.83 18599.341 663.73 97.31 96.87 2434.84 48696.74 1.63 5.4
Total 19112.978 685.15 100.00 100.00
2/25/2014 11:20 AM Page 2 of 2Chromatogram L:\int21140.PRM
-
MT-605Riboflavin, [3H]-
Lot 213-021-0212-A-20090119-JG
Chromatogram Info:
File Name : L:\int21140 File Created : 2/25/2014 10:51:06 AM
Origin : Acquired, Acquisition started 5/18/2011 8:52:45 AM Acquired Date : 5/18/2011 9:12:45 AM
Project : Test By : Administrator
Method : unit2-20minrun By : Administrator
Description : UV trace of 3H material co-injected with standard
Created : 8/8/2007 9:12 AM Modified : 2/25/2014 11:06 AM
Column : Detection : UV 266nm
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
5 10 15 20
[mV]
Volta
ge
0
100
200
300
400
4.48
1
5.19
2
7.62
3
8.35
4
9.22
5
9.66
6
L:\ int 21140 - Unit 2 UV
2/25/2014 11:06 AM Page 1 of 2Chromatogram L:\int21140.PRM
-
Result Table (Uncal - L:\int21140 - Unit 2 UV)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
Eff/l[t.p./m]
Symmetry/Tailing[-]
Response Factor
Resolution[-]
1 4.48 16.261 2.05 0.38 0.60 8156.91 163138.21 0.87
2 5.19 9.685 1.03 0.22 0.30 9312.74 186254.80 0.81 3.5
3 7.62 4261.148 335.20 98.66 98.40 9919.61 198392.23 1.32 9.3
4 8.35 24.561 1.76 0.57 0.52 8480.44 169608.83 1.21 2.2
5 9.22 2.935 0.27 0.07 0.08 15675.00 313499.91 0.92 2.7
6 9.66 4.291 0.35 0.10 0.10 13840.45 276809.00 1.17 1.4
Total 4318.880 340.66 100.00 100.00
2/25/2014 11:06 AM Page 2 of 2Chromatogram L:\int21140.PRM
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MT-605Riboflavin, [3H]-
Lot 213-021-0212-A-20090119-JG
Chromatogram Info:
File Name : L:\int21136 File Created : 2/25/2014 10:51:06 AM
Origin : Acquired, Acquisition started 5/17/2011 1:53:13 PM Acquired Date : 5/17/2011 2:13:13 PM
Project : Test By : Administrator
Method : unit2-20minrun By : Administrator
Description : UV trace of blank injection
Created : 8/8/2007 9:12 AM Modified : 2/25/2014 11:21 AM
Column : Detection : UV 266nm
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
5 10 15 20
[mV]
Volta
ge
-5
0
5
10
15
L:\ int 21136 - Unit 2 UV
2/25/2014 11:21 AM Page 1 of 2Chromatogram L:\int21136.PRM
-
Result Table (Uncal - L:\int21136 - Unit 2 UV)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
Eff/l[t.p./m]
Symmetry/Tailing[-]
Response Factor
Resolution[-]
No peak to report
2/25/2014 11:21 AM Page 2 of 2Chromatogram L:\int21136.PRM
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C:\Xcalibur\data\MT605-20090119-JG 8/4/2010 11:38:12 AM MT605-20090119-JG
RT: 0.00 - 1.99
0.0 0.5 1.0 1.5
Time (min)
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
1.96
1.731.65
1.511.42
1.241.130.870.510.47
0.23
NL:9.39E6
TIC F: MS MT605-20090119-JG
MT605-20090119-JG#1-157 RT: 0.00-1.99 AV:T: + c Q3MS [ 200.00-800.00]m/z= 371.57-400.28
m/z Intensity Relativem/z Intensity Relative
377.25 2254794.5 100.00 378.26 481660.2 21.36 379.26 548537.5 24.33 380.27 111380.5 4.94 381.27 194303.4 8.62 382.28 39598.4 1.76 383.28 174679.4 7.75 384.28 35987.5 1.60 385.29 39608.1 1.76 386.31 8305.5 0.37 387.29 33648.2 1.49 388.31 9008.4 0.40 389.30 72512.4 3.22 390.28 15106.0 0.67 391.36 14597.3 0.65 392.34 4579.3 0.20 393.28 4667.2 0.21 395.27 4999.3 0.22 399.23 43137.4 1.91 400.23 9479.1 0.42
MT605-20090119-JG #1-157 RT: 0.00-1.99 AV: 157 NL: 2.25E6T: + c Q3MS [ 200.00-800.00]
200 300 400 500 600 700 800
m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
377.25
217.26 753.35
243.21389.30
287.30 469.31 593.25 634.98
MT605-20090119-JG #1-157 RT: 0.00-1.99 AV: 157 NL: 2.25E6T: + c Q3MS [ 200.00-800.00]
360 380 400 420 440
m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
377.25
379.26
381.27
389.30399.23 411.26 455.29423.30371.32 445.28
-
98
76
54
32
1pp
m
2.3142.4172.4672.523
1.00
PC 1.00
GB 0
LB 0.00 Hz
SSB 0
WDW no
SF 320.1305850 MHz
SI 32768
SFO1 320.1321857 MHz
PL1 2.00 dB
P1 10.00 usec
NUC1 3H
======== CHANNEL f1 ========
TD0 1
D1 2.00000000 sec
TE 300.0 K
DE 6.00 usec
DW 81.000 usec
RG 46341
AQ 1.3271540 sec
FIDRES 0.376760 Hz
SWH 6172.839 Hz
DS 2
NS 5000
SOLVENT D2O
TD 16384
PULPROG zg
PROBHD 5 mm DUX 3H−1H
INSTRUM spect
Time 15.20
Date_ 20110520
PROCNO 1
EXPNO 2
NAME MT605−20090119−JG
Batch 20090119−JG
MT605 3H NMR in D2O
Table of contents for MT605 Lot 213-021-0212-A-20090119-JGint21138radint21138uvint21139uvStdint21140radCoint21140uvCoint21136uvBlkMT605-20090119-JG-MS-2mt605-20090119-jg-nmr-1