MULTIPLE SEQUENCES ALIGNMENT OF OF KERATIN PROTEIN OF DIFFERENT SPECIES

STEPS TO ALIGN THE SEQUENCES.

1) To retrieve the sequences, first go to NCBI website.

1

2) The sequences of keratin protein of different species.

>gi|165447|gb|AAA31379.1| keratin [Oryctolagus cuniculus]MCGYYGNYYGGRGYGCCGYGGLGYGYGGLGCGLGSYYGCGYRRLGCGYGCGYGYGYGSRSLCGCGYGYGSGYGSGFGYYY

>gi|165953|gb|AAA31554.1| keratin [Ovis aries]MCGYYGNYYGGLGCGSYSYGGLGCGYGSCYGSGFRRLGCGYGCGYGYGSRSLCGSGYGYGSRSLCGSGYGCGSGYGSGFGYYY

>gi|386848|gb|AAB59562.1| keratin [Homo sapiens]MTTCSRQFTSSSSMKGSCGIGGGIGAGSSRISSVLAGGSCRAPNTYGGGLSVSSSRFSSGGAYGLGGGYGGGFSSSSSSFGSGFGGGYGGGLGAGLGGGFGGGFAGGDGLLVGSEKVTMQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPAEIKDYSPYFKTIEDLRNKILTATVDNANVLLQIDNARLAADDFRTKYETELNLRMSVEADINGLRRVLDELTLARADLEMQIESLKEELAYLKKNHEEEMNALRGQVGGDVNVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEEWFFTKTEELNREVATNSELVQSGKSEISELRRTMQNLEIELQSQLSMKASLENSLEETKGRYCMQLAQIQEMIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLSSSQFSSGSQSSRDVTSSSRQIRTKVMDVHDGKVVSTHEQVLRTKN

>gi|45383265|ref|NP_989779.1| keratin [Gallus gallus]MSCYDLCAPACGVATPAPLADSCNEPCVRQCPDSTVVIQPPATVVTFPGPILSSFPQNAVVGSAGVPAVGSGMGGTFGRGAGFGGYGGLGGYGGYGGLGGYGGYGGFYGLGGYGGYGSCGYGGWSRGHRYLSGSCGPC

>gi|118102129|ref|XP_428872.2| PREDICTED: keratin [Gallus gallus]MSCYDLCAPTPCGPTPLANSCNEPCVRQCQDSTVVIQPSPVVVTLPGPILSSFPQNTTVGSSASAAVGSALSAGGVPISSGSSLGFGSLGYPGLGSGYSRPYRRYNTYRSGFNGPC

>gi|2338550|gb|AAB67167.1| keratin [Sparus aurata]KTRQFPEAKTAPQAHDLCGFSAPYHRHAGPDLSSYSWKRRHHPLHRQRQAGQRRLQSQVRERADHASVGGGRHRRAEEGSGRADSGEIRPGDAGGESEEGLFHLKKNHEEDLLALRAQMGGQVNVGVDAAPQQDLSVVMAGIGEHYETVSNKNRNELESWFQAKSAELNKEVAVRTETLETSRSSISEIRRTYQSLENQLQAAVSERGAL

2

EGTLAETKSRYSRMLAGYQRQVVSLEEQLPSLRANLENQRGLYSELLDIKTRLELEIAEYRRLLDGNLDTTTTKTTTTTTRRVVTIVQEVDENGKVISS

>gi|64858|emb|CAA28374.1| keratin [Xenopus laevis]MTSYRSSSASYYSGSSSKGGFGSRSLAGSNSYGGSSFGAGFSSGVGSGFSSSGGNFAMAEAASSSFGGNEKHAMQNLNDRLASYLEKVRALEATNSDLEGKIRNWYDKQSDAGIGAGSKDYSKYFEIIAELRNKIRAATIDNATVTLQIDNARLAADDFRLKFENELALRQSVEGDSNGLRRVLDELILARGDFELQIESLTEELAYLKKNHEEEMSHAKSQSAGKVSVEMDAALGVDLTSILNNMRADYEILAEKNRRDAELWFNQKSGELKKEISVGVEQVQASKSEITELKRSLQSLEIELQSQLAMKQSVEGNLNELQGFYSSQLQQIQNTIGSLEEQLLQIRSDMEHQNTEYKLLLDIKTRLEMEIQTYRRLLEGELGQVTTVANTSSVESKTESSSTSTTRTRMVKTIVEEVVDGKVVSSRVE

>gi|348041268|ref|NP_001011879.2| keratin [Takifugu rubripes]MSVRAVKTTTLSSVSTSRGPSQGFSSRSFSGYGGYGVGGGRQSFAVRSSYGGLGSSGAAVGAGGFKVVGGYVAGGAGNRVGGLECGYIGFGGGVAGGMGNDVVAPITAVTVNKSLLAPLNLEIDPTIQAIRTQEKEQIKTLNNRFASFIDKVRFLEQQNKMLETKWKLLQEQTTSRSNIDMMFEAYIANLRKHLDNLGHEKVKLESDLHHMTDLVEDYKTRYEDEINKRTDCENNFVLIKKDADAAYMTKVDLETQVTALSDELDFLRQIYDVEIQELQGQIKDTCVVVEMDNSRDLDMDSIVAEVRAQYEDVANRSRAEAETWYQTKYAEMQQSAGRYGDDLKLSKAEISDMNRRIMRLQSEIDMVKSQKNHLEAQIAESEERGELAVTDAKHRIRELEEALQRAKQDMTMQVRQYQELMNVKLALDIEIATYRKLLEGEEDRLATGIKTTVSKQTSMNFSAYGLESSRTPSYVSSSFGAVKISGGPVSAQEATAMEHINKTETKVIKAEGAQKEEQVAQEVSAAVEEQTSEEKQEQKQEQQVVEAEAVVEE

>gi|2352260|gb|AAC26971.1| keratin [Canis lupus familiaris]MNCRQFLSSHCSRDSSGGGGGSKMFSCNRSSFSGASGGGGRFSSSRSFGGGSSGACGRGGGGSFGSSYGGGSGGGFSAGSFGGHSRGFSGGSGGGFGGGFGGGFGGFSGGSGGGFGGPGGFGSGFDSGAGGILGADEKTTMQDLNSRLASYLDKVQALEDANKELESKIREWYDKQGSRTFHRDYSPYYDTIEDLKNQIVNITADNNKTLLDLDNTRMTLDDIRMKCDIENNLRLAVNADINGLRKVLDDLTMQKSDLEMHYESLQEELIALKKNHEDEMSQLTGQNSGDVNVEMNAAPGRDLTKILNDMREEYERISAKNRKDIEEQYETQMSQMEQEVMSSGQEMESNHKEVTQLRHSIQEMEIELQSQLSKKSALEKSLEDTKNHYCGQLQQLQEQIRSLEGQITEIRGEIECQNQEYSLLLNIKTRLEQEIKTYRSLLEGGQEDFESHESGQSHFGSGGSRQQGGIGGSHGRGSRGGSGGSYGGGSSSGGGSGGSHGGGSGGSYGGGSSSGGGSGGRGGSGGSYGGGSGSGGGSSGSYGGGSSSGGGSGGSHGGGS

3

GGSYGGGSSSGGGSGGRGGSGGSYGGGSGSGGGRGGGCEEGSGSGGRSGGSYGGGSGSGGGSSCSYGGGSSSGGGSGGSYGGGSSSGGGSGGKGGSGCSYSGGSSSGGGSGGSYGGGSSSGRGSGGRGGSAGSYGGGSGSGGGRGGGCEEGSGSGGRSGGSYGGGSGSGGRSGGSYGGGSGSGGGSGGSYGGGSGSKGGSGRSSQVQSSSSKSGECDDTQGYHIQY

>gi|10716084|dbj|BAB16373.1| keratin [Paralichthys olivaceus]KNQRELEAWLQTQSESLSKEVAVKTEVLQTTKAEISDLRRTMQNLEIELQSQLSMKAALEGTLSDTECRYSMQLQSLQIQVTSLEEQLMQLRADMERQNQEYNILLDIKTRLEMEIAEY

3) TO ALIGN THE SEQUENCES GO TO EMBL- EBI WEBSITE.

4

4) NOW SELECT THE CustalW2 TOOL FOR ALIGN THE SEQUENCES.

5

ALIGNMENT OF THE SEQUENCES OF DIFFERENT SPECIES.

gi|165447|gb|AAA31379.1| --------------------------------------------------gi|165953|gb|AAA31554.1| --------------------------------------------------gi|386848|gb|AAB59562.1| ----MTTCSRQFTSSSSMKGSCGIGGGIGAGSSRISSVLAGGSCRAPNTY 46gi|64858|emb|CAA28374.1| ----MTSYR---SSSASYYSGSSSKGGFGSRS------LAG-----SNSY 32gi|2338550|gb|AAB67167.1| --------------------------------------------------gi|10716084|dbj|BAB16373.1| --------------------------------------------------gi|2352260|gb|AAC26971.1| --MNCRQFLSSHCSRDSSGGGGGSKMFSCNRSSFSGASGGGGRFSSSRSF 48gi|45383265|ref|NP_989779.1| --------------------------------------------------gi|118102129|ref|XP_428872.2| --------------------------------------------------gi|348041268|ref|NP_001011879. MSVRAVKTTTLSSVSTSRGPSQGFSSRSFSGYGGYGVGGGRQSFAVRSSY 50

gi|165447|gb|AAA31379.1| --------------------------------------------------gi|165953|gb|AAA31554.1| --------------------------------------------------gi|386848|gb|AAB59562.1| GGGLSVSSSRFSSGGA-YGLGGGYGGGFSS-SSSSFGSGFGGGYGGGLGA 94gi|64858|emb|CAA28374.1| GG------SSFGAG-----FSSGVGSGFSS-SGGNF----------AMAE 60gi|2338550|gb|AAB67167.1| --------------------------------------------------gi|10716084|dbj|BAB16373.1| --------------------------------------------------gi|2352260|gb|AAC26971.1| GGGSSGACGRGGGGSFGSSYGGGSGGGFSAGSFGGHSRGFSGGSGGGFGG 98gi|45383265|ref|NP_989779.1| --------------------------------------------------gi|118102129|ref|XP_428872.2| --------------------------------------------------gi|348041268|ref|NP_001011879. GGLGSSGAAVGAGGFKVVGGYVAGGAGNRVGGLECGYIGFGGGVAGGMGN 100

gi|165447|gb|AAA31379.1| --------------------------------------------------gi|165953|gb|AAA31554.1| --------------------------------------------------gi|386848|gb|AAB59562.1| GLGGGFG------------------GGFAGGDGLLVGSEKVTMQNLNDRL 126gi|64858|emb|CAA28374.1| AASSSFG------------------G-----------NEKHAMQNLNDRL 81

6

gi|2338550|gb|AAB67167.1| ----------------------------------------KTRQFPEAKT 10gi|10716084|dbj|BAB16373.1| --------------------------------------------------gi|2352260|gb|AAC26971.1| GFGGGFGGFSGGSGGGFGGPGGFGSGFDSGAGGILGADEKTTMQDLNSRL 148gi|45383265|ref|NP_989779.1| --------------------------------------------------gi|118102129|ref|XP_428872.2| --------------------------------------------------gi|348041268|ref|NP_001011879. DVVAPITAVTVNKSLLAPLNLEIDP-----TIQAIRTQEKEQIKTLNNRF 145

gi|165447|gb|AAA31379.1| --------------------------------------------------gi|165953|gb|AAA31554.1| --------------------------------------------------gi|386848|gb|AAB59562.1| ASYLDKVRALEEANADLEVKIRDWYQRQRPAEI----KDYSPYFKTIEDL 172gi|64858|emb|CAA28374.1| ASYLEKVRALEATNSDLEGKIRNWYDKQSDAGIGAGSKDYSKYFEIIAEL 131gi|2338550|gb|AAB67167.1| APQAHDLCGFSAP-----------YHRHAGPDL----SSYSWKRRHHPLH 45gi|10716084|dbj|BAB16373.1| --------------------------------------------------gi|2352260|gb|AAC26971.1| ASYLDKVQALEDANKELESKIREWYDKQGSRTFH---RDYSPYYDTIEDL 195gi|45383265|ref|NP_989779.1| --------------------------------------------------gi|118102129|ref|XP_428872.2| --------------------------------------------------gi|348041268|ref|NP_001011879. ASFIDKVRFLEQQNKMLETKWKLLQEQTTSRSN-----IDMMFEAYIANL 190

gi|165447|gb|AAA31379.1| --------------------------------------------------gi|165953|gb|AAA31554.1| --------------------------------------------------gi|386848|gb|AAB59562.1| RNKILTATVDNANVLLQIDNARLAADDFRTKYETELNLRMSVEADINGLR 222gi|64858|emb|CAA28374.1| RNKIRAATIDNATVTLQIDNARLAADDFRLKFENELALRQSVEGDSNGLR 181gi|2338550|gb|AAB67167.1| RQRQAGQRRLQSQVRERADHASVGGGRHRRAEE------GSGRADSGEIR 89gi|10716084|dbj|BAB16373.1| --------------------------------------------------gi|2352260|gb|AAC26971.1| KNQIVNITADNNKTLLDLDNTRMTLDDIRMKCDIENNLRLAVNADINGLR 245gi|45383265|ref|NP_989779.1| --------------------------------------------------gi|118102129|ref|XP_428872.2| --------------------------------------------------gi|348041268|ref|NP_001011879. RKHLDNLGHEKVKLESDLHHMTDLVEDYKTRYEDEINKRTDCENNFVLIK 240

gi|165447|gb|AAA31379.1| --------------------------------------------------gi|165953|gb|AAA31554.1| --------------------------------------------------gi|386848|gb|AAB59562.1| RVLDELTLARADLEMQIESLKEELAYLKKNHEEEMNALRGQVGG-DVNVE 271gi|64858|emb|CAA28374.1| RVLDELILARGDFELQIESLTEELAYLKKNHEEEMSHAKSQSAG-KVSVE 230gi|2338550|gb|AAB67167.1| ---------PGDAGGESE---EGLFHLKKNHEEDLLALRAQMGG-QVNVG 126gi|10716084|dbj|BAB16373.1| --------------------------------------------------

7

gi|2352260|gb|AAC26971.1| KVLDDLTMQKSDLEMHYESLQEELIALKKNHEDEMSQLTGQNSG-DVNVE 294gi|45383265|ref|NP_989779.1| --------------------------------------------------gi|118102129|ref|XP_428872.2| --------------------------------------------------gi|348041268|ref|NP_001011879. KDADAAYMTKVDLETQVTALSDELDFLRQIYDVEIQELQGQIKDTCVVVE 290

gi|165447|gb|AAA31379.1| ------------------------------MCGYYGNYYGGRGYGC---- 16gi|165953|gb|AAA31554.1| ------------------------------MCGYYGNYYGGLG------- 13gi|386848|gb|AAB59562.1| MDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEEWFFTKTEELNREVATNS 321gi|64858|emb|CAA28374.1| MDAALGVDLTSILNNMRADYEILAEKNRRDAELWFNQKSGELKKEISVGV 280gi|2338550|gb|AAB67167.1| VDAAPQQDLSVVMAGIGEHYETVSNKNRNELESWFQAKSAELNKEVAVRT 176gi|10716084|dbj|BAB16373.1| -------------------------KNQRELEAWLQTQSESLSKEVAVKT 25gi|2352260|gb|AAC26971.1| MNAAPGRDLTKILNDMREEYERISAKNRKDIEEQYETQMSQMEQEVMSSG 344gi|45383265|ref|NP_989779.1| ---MSCYDLCAP--ACGVATPAPLADSCNEPCVRQCPDSTVVIQPPATVV 45gi|118102129|ref|XP_428872.2| ---MSCYDLCAPT-PCG---PTPLANSCNEPCVRQCQDSTVVIQPSPVVV 43gi|348041268|ref|NP_001011879. MDNSRDLDMDSIVAEVRAQYEDVANRSRAEAETWYQTKYAEMQQSAGRYG 340

gi|165447|gb|AAA31379.1| -------CGYGGLGYGYGGLGCGLGSYYG------CGYRRLGCGYG---C 50gi|165953|gb|AAA31554.1| -------CG----SYSYGGLGCGYGSCYG------SGFRRLGCGYG---C 43gi|386848|gb|AAB59562.1| ELVQSGKSEISELRRTMQNLEIELQSQLSMKASLENSLEETKGRYCMQLA 371gi|64858|emb|CAA28374.1| EQVQASKSEITELKRSLQSLEIELQSQLAMKQSVEGNLNELQGFYSSQLQ 330gi|2338550|gb|AAB67167.1| ETLETSRSSISEIRRTYQSLENQLQAAVSERGALEGTLAETKSRYSRMLA 226gi|10716084|dbj|BAB16373.1| EVLQTTKAEISDLRRTMQNLEIELQSQLSMKAALEGTLSDTECRYSMQLQ 75gi|2352260|gb|AAC26971.1| QEMESNHKEVTQLRHSIQEMEIELQSQLSKKSALEKSLEDTKNHYCGQLQ 394gi|45383265|ref|NP_989779.1| TFPGPILSSFPQNAVVGSAGVPAVGSGMGGTFGRGAGFGG-YGGLGGYGG 94gi|118102129|ref|XP_428872.2| TLPGPILSSFPQNTTVGSSASAAVGSALS---AGGVPISS-GSSLG---- 85gi|348041268|ref|NP_001011879. DDLKLSKAEISDMNRRIMRLQSEIDMVKSQKNHLEAQIAESEERGELAVT 390 .

gi|165447|gb|AAA31379.1| GYGYG----------YGSRSLCGCG-Y----GYGSGYGSGFGYYY----- 80

8

gi|165953|gb|AAA31554.1| GYGYGSRSLCGSGYGYGSRSLCGSG-Y----GCGSGYGSGFGYYY----- 83gi|386848|gb|AAB59562.1| QIQEMIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEG 421gi|64858|emb|CAA28374.1| QIQNTIGSLEEQLLQIRSDMEHQNTEYKLLLDIKTRLEMEIQTYRRLLEG 380gi|2338550|gb|AAB67167.1| GYQRQVVSLEEQLPSLRANLENQRGLYSELLDIKTRLELEIAEYRRLLDG 276gi|10716084|dbj|BAB16373.1| SLQIQVTSLEEQLMQLRADMERQNQEYNILLDIKTRLEMEIAEY------ 119gi|2352260|gb|AAC26971.1| QLQEQIRSLEGQITEIRGEIECQNQEYSLLLNIKTRLEQEIKTYRSLLEG 444gi|45383265|ref|NP_989779.1| YGGLGGYGGYGGFYGLGGYGGYGSCGYGGWSRGHRYLSGSCGPC------ 138gi|118102129|ref|XP_428872.2| FGSLG-------YPGLG--SGY-SRPYR---RYNTYRSGFNGPC------ 116gi|348041268|ref|NP_001011879. DAKHRIRELEEALQRAKQDMTMQVRQYQELMNVKLALDIEIATYRKLLEG 440 *

gi|165447|gb|AAA31379.1| --------------------------------------------------gi|165953|gb|AAA31554.1| --------------------------------------------------gi|386848|gb|AAB59562.1| EDAHLS-----SSQFSSGSQSSRDVTSSSRQIRTKVMDVHD-GKVVSTHE 465gi|64858|emb|CAA28374.1| ELGQVTTV-ANTSSVESKTESSSTSTTRTRMVKTIVEEVVD-GKVVSSRV 428gi|2338550|gb|AAB67167.1| ---------------NLDTTTTKTTTTTTRRVVTIVQEVDENGKVISS-- 309gi|10716084|dbj|BAB16373.1| --------------------------------------------------gi|2352260|gb|AAC26971.1| GQEDFESHESGQSHFGSGGSRQQGGIGGSHGRGSRGGSGGSYGGGSSSGG 494gi|45383265|ref|NP_989779.1| --------------------------------------------------gi|118102129|ref|XP_428872.2| --------------------------------------------------gi|348041268|ref|NP_001011879. EEDRLATGIKTTVSKQTSMNFSAYGLESSRTPSYVSSSFGAVKISGGPVS 490

gi|165447|gb|AAA31379.1| --------------------------------------------------gi|165953|gb|AAA31554.1| --------------------------------------------------gi|386848|gb|AAB59562.1| QVLRTKN------------------------------------------- 472gi|64858|emb|CAA28374.1| E------------------------------------------------- 429gi|2338550|gb|AAB67167.1| --------------------------------------------------gi|10716084|dbj|BAB16373.1| --------------------------------------------------gi|2352260|gb|AAC26971.1| GSGGSHGGGSGGSYGGGSSSGGGSGGRGGSGGSYGGGSGSGGGSSGSYGG 544gi|45383265|ref|NP_989779.1| --------------------------------------------------gi|118102129|ref|XP_428872.2| --------------------------------------------------gi|348041268|ref|NP_001011879. AQEATAMEHINKTETKVIKAEGAQKEEQVAQEVSAAVEEQTSEEKQEQKQ 540

gi|165447|gb|AAA31379.1| --------------------------------------------------gi|165953|gb|AAA31554.1| --------------------------------------------------gi|386848|gb|AAB59562.1| --------------------------------------------------

9

gi|64858|emb|CAA28374.1| --------------------------------------------------gi|2338550|gb|AAB67167.1| --------------------------------------------------gi|10716084|dbj|BAB16373.1| --------------------------------------------------gi|2352260|gb|AAC26971.1| GSSSGGGSGGSHGGGSGGSYGGGSSSGGGSGGRGGSGGSYGGGSGSGGGR 594gi|45383265|ref|NP_989779.1| --------------------------------------------------gi|118102129|ref|XP_428872.2| --------------------------------------------------gi|348041268|ref|NP_001011879. EQQVVEAEAVVEE------------------------------------- 553

gi|165447|gb|AAA31379.1| --------------------------------------------------gi|165953|gb|AAA31554.1| --------------------------------------------------gi|386848|gb|AAB59562.1| --------------------------------------------------gi|64858|emb|CAA28374.1| --------------------------------------------------gi|2338550|gb|AAB67167.1| --------------------------------------------------gi|10716084|dbj|BAB16373.1| --------------------------------------------------gi|2352260|gb|AAC26971.1| GGGCEEGSGSGGRSGGSYGGGSGSGGGSSCSYGGGSSSGGGSGGSYGGGS 644gi|45383265|ref|NP_989779.1| --------------------------------------------------gi|118102129|ref|XP_428872.2| --------------------------------------------------gi|348041268|ref|NP_001011879. --------------------------------------------------

gi|165447|gb|AAA31379.1| --------------------------------------------------gi|165953|gb|AAA31554.1| --------------------------------------------------gi|386848|gb|AAB59562.1| --------------------------------------------------gi|64858|emb|CAA28374.1| --------------------------------------------------gi|2338550|gb|AAB67167.1| --------------------------------------------------gi|10716084|dbj|BAB16373.1| --------------------------------------------------gi|2352260|gb|AAC26971.1| SSGGGSGGKGGSGCSYSGGSSSGGGSGGSYGGGSSSGRGSGGRGGSAGSY 694gi|45383265|ref|NP_989779.1| --------------------------------------------------gi|118102129|ref|XP_428872.2| --------------------------------------------------gi|348041268|ref|NP_001011879. --------------------------------------------------

gi|165447|gb|AAA31379.1| --------------------------------------------------gi|165953|gb|AAA31554.1| --------------------------------------------------gi|386848|gb|AAB59562.1| --------------------------------------------------gi|64858|emb|CAA28374.1| --------------------------------------------------gi|2338550|gb|AAB67167.1| --------------------------------------------------gi|10716084|dbj|BAB16373.1| --------------------------------------------------gi|2352260|gb|AAC26971.1| GGGSGSGGGRGGGCEEGSGSGGRSGGSYGGGSGSGGRSGGSYGGGSGSGG 744gi|45383265|ref|NP_989779.1| --------------------------------------------------gi|118102129|ref|XP_428872.2| --------------------------------------------------gi|348041268|ref|NP_001011879. --------------------------------------------------

gi|165447|gb|AAA31379.1| ------------------------------------------gi|165953|gb|AAA31554.1| ------------------------------------------gi|386848|gb|AAB59562.1| ------------------------------------------gi|64858|emb|CAA28374.1| ------------------------------------------gi|2338550|gb|AAB67167.1| ------------------------------------------

10

gi|10716084|dbj|BAB16373.1| ------------------------------------------gi|2352260|gb|AAC26971.1| GSGGSYGGGSGSKGGSGRSSQVQSSSSKSGECDDTQGYHIQY 786gi|45383265|ref|NP_989779.1| ------------------------------------------gi|118102129|ref|XP_428872.2| ------------------------------------------gi|348041268|ref|NP_001011879. ------------------------------------------

RESULT SUMMRAY

SeqA

NameLengt

hSeqB

NameLengt

hScor

e1 gi|165447|gb|AAA31379.1| 80 2 gi|165953|gb|AAA31554.1| 83 78.01 gi|165447|gb|AAA31379.1| 80 3 gi|386848|gb|AAB59562.1| 472 35.0

1 gi|165447|gb|AAA31379.1| 80 4gi|45383265|ref|NP_989779.1|

138 37.0

1 gi|165447|gb|AAA31379.1| 80 5gi|118102129|ref|XP_428872.2|

116 15.0

1 gi|165447|gb|AAA31379.1| 80 6 gi|2338550|gb|AAB67167.1| 309 6.01 gi|165447|gb|AAA31379.1| 80 7 gi|64858|emb|CAA28374.1| 429 16.0

11

SeqA

NameLengt

hSeqB

NameLengt

hScor

e

1 gi|165447|gb|AAA31379.1| 80 8gi|348041268|ref|NP_001011879.2|

553 28.0

1 gi|165447|gb|AAA31379.1| 80 9 gi|2352260|gb|AAC26971.1| 786 43.0

1 gi|165447|gb|AAA31379.1| 80 10gi|10716084|dbj|BAB16373.1|

119 5.0

2 gi|165953|gb|AAA31554.1| 83 3 gi|386848|gb|AAB59562.1| 472 32.0

2 gi|165953|gb|AAA31554.1| 83 4gi|45383265|ref|NP_989779.1|

138 30.0

2 gi|165953|gb|AAA31554.1| 83 5gi|118102129|ref|XP_428872.2|

116 13.0

2 gi|165953|gb|AAA31554.1| 83 6 gi|2338550|gb|AAB67167.1| 309 13.02 gi|165953|gb|AAA31554.1| 83 7 gi|64858|emb|CAA28374.1| 429 24.0

2 gi|165953|gb|AAA31554.1| 83 8gi|348041268|ref|NP_001011879.2|

553 25.0

2 gi|165953|gb|AAA31554.1| 83 9 gi|2352260|gb|AAC26971.1| 786 42.0

2 gi|165953|gb|AAA31554.1| 83 10gi|10716084|dbj|BAB16373.1|

119 4.0

3 gi|386848|gb|AAB59562.1| 472 4gi|45383265|ref|NP_989779.1|

138 21.0

3 gi|386848|gb|AAB59562.1| 472 5gi|118102129|ref|XP_428872.2|

116 8.0

3 gi|386848|gb|AAB59562.1| 472 6 gi|2338550|gb|AAB67167.1| 309 34.03 gi|386848|gb|AAB59562.1| 472 7 gi|64858|emb|CAA28374.1| 429 57.0

3 gi|386848|gb|AAB59562.1| 472 8gi|348041268|ref|NP_001011879.2|

553 28.0

3 gi|386848|gb|AAB59562.1| 472 9 gi|2352260|gb|AAC26971.1| 786 48.0

3 gi|386848|gb|AAB59562.1| 472 10gi|10716084|dbj|BAB16373.1|

119 62.0

4gi|45383265|ref|NP_989779.1|

138 5gi|118102129|ref|XP_428872.2|

116 58.0

4gi|45383265|ref|NP_989779.1|

138 6 gi|2338550|gb|AAB67167.1| 309 12.0

4gi|45383265|ref|NP_989779.1|

138 7 gi|64858|emb|CAA28374.1| 429 7.0

4gi|45383265|ref|NP_989779.1|

138 8gi|348041268|ref|NP_001011879.2|

553 15.0

4gi|45383265|ref|NP_989779.1|

138 9 gi|2352260|gb|AAC26971.1| 786 27.0

4gi|45383265|ref|NP_989779.1|

138 10gi|10716084|dbj|BAB16373.1|

119 3.0

5gi|118102129|ref|XP_428872.2|

116 6 gi|2338550|gb|AAB67167.1| 309 4.0

12

SeqA

NameLengt

hSeqB

NameLengt

hScor

e

5gi|118102129|ref|XP_428872.2|

116 7 gi|64858|emb|CAA28374.1| 429 11.0

5gi|118102129|ref|XP_428872.2|

116 8gi|348041268|ref|NP_001011879.2|

553 9.0

5gi|118102129|ref|XP_428872.2|

116 9 gi|2352260|gb|AAC26971.1| 786 12.0

5gi|118102129|ref|XP_428872.2|

116 10gi|10716084|dbj|BAB16373.1|

119 7.0

6 gi|2338550|gb|AAB67167.1| 309 7 gi|64858|emb|CAA28374.1| 429 33.0

6 gi|2338550|gb|AAB67167.1| 309 8gi|348041268|ref|NP_001011879.2|

553 20.0

6 gi|2338550|gb|AAB67167.1| 309 9 gi|2352260|gb|AAC26971.1| 786 26.0

6 gi|2338550|gb|AAB67167.1| 309 10gi|10716084|dbj|BAB16373.1|

119 57.0

7 gi|64858|emb|CAA28374.1| 429 8gi|348041268|ref|NP_001011879.2|

553 24.0

7 gi|64858|emb|CAA28374.1| 429 9 gi|2352260|gb|AAC26971.1| 786 41.0

7 gi|64858|emb|CAA28374.1| 429 10gi|10716084|dbj|BAB16373.1|

119 55.0

8gi|348041268|ref|NP_001011879.2|

553 9 gi|2352260|gb|AAC26971.1| 786 21.0

8gi|348041268|ref|NP_001011879.2|

553 10gi|10716084|dbj|BAB16373.1|

119 30.0

9 gi|2352260|gb|AAC26971.1| 786 10gi|10716084|dbj|BAB16373.1|

119 47.0

13

Functions of keratin protein.

Keratin is a tough fibrous protein that strengthens skin, hair and nails with its tight strands and intertwined structures. There are two types of keratin, according to Bio-Medicine.org: alpha-keratin, which is abundant in humans and mammals, and beta-keratin that is primarily in birds and reptiles.

Functions

Strengthens hair

Keratin is a major component responsible for strengthening the hair, making it less likely to break.

Coats and repairs damaged hair

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Keratin helps to smooth and coat frizzy and frayed hair shafts, resulting in smoother and straighter hair. It repairs damaged hair by smoothing and coating the hair shafts and helps to keep in moisture.

Maintains healthy skin

Keratin is also contained in the outer epidermis and creates the tough skin in calluses. It helps skin maintain its elasticity and firmness. Keratin controls cell growth and renewal, which helps to soften and control wrinkles.

Skin pigmentation and protection

Keratin has an influence on melanin and skin pigmentation. The protein plays a part in waterproofing skin, according to NaturalSkinHealth.com. It also creates a slight barrier against bacteria and other organisms.

Toughens nails and bony structures

Nails are made up of keratin, which causes them to become less prone to chipping and breaking. Keratin is a major part of hardened structures of animals such as horns, hoofs, beaks and even feathers.

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