nanoparticles with immobilized biosensors for … wet strength paper that’s all bioactivepaper.com...
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Nanoparticles with Immobilized Biosensors for Bioactive Papers
Shunxing Su, Razvan Nutiu, Carlos D. M. Filipe, Yingfu Li, Robert Pelton
McMaster Centre for Pulp and Paper ResearchDepartment of Chemical Engineering
McMaster University1280 Main St. W., Hamilton, Ontario, Canada L8S 4L7
E-mail: [email protected]
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Imagine a Imagine a BioactiveBioactive Paper TowelPaper Towel
That would change color to tell us when our kitchen counter top was contaminated with dangerous bacteria.That would change color to warn us of the presence of anthrax spores.
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Imagine a Bioactive MaskImagine a Bioactive Mask
Which will:owarn the user of
viral contamination.ocapture and
deactivate the virus.
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Imagine a Bioactive Water FilterImagine a Bioactive Water FilterSpecifically bind small, soluble (i.e. difficult to remove) toxinsCapture and kill all bacteria and virus.Warn user of contamination and when the cartridge must be changed.
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SENTINEL Bioactive Paper NetworkBioactive Paper Network
a Network of Canadian AcademicsNetwork of Canadian Academics~ 28 professors, 11 Universities, ~ 70 total students,
PDFs and profs.~ $2.5 million / year
o 75% from Canadian Government’s Natural Science and Engineering Research Council (NSERC Network)
o 25% cash from industrial consortium and Ontario Government.
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The The SENTINEL VisionVision
Bioactive Paper - inexpensive paper products which detect and repel or deactivate waterborne and airborne pathogens.
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Key Elements of Key Elements of SENTINEL VisionVision
υ Paper giving instant visible indication of pathogens.
υ High speed manufacture – coating or printing.
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Bioactive Paper Bioactive Paper –– a a ““TopTop”” ideaidea
New York Times, Sunday Magazine Dec 9, 2007Described the Sentinel Bioactive paper concept as one of the top 70 in “The 7th
Annual Year in Ideas”
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““detectdetect”” pathogenspathogens
Instant, visible indication of pathogens without instrumentationTechnologies do not exist – this is the “killer application” for bioactive paperA large part of the SENTINEL research
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Sentinel Biodetection PlatformsSentinel Biodetection PlatformsAgents which specifically recognize(capture) a targetSentinel researchers are developing four technologies in parallelo Antibodieso Bacteriophageo DNA aptamerso Enzymes
BiodetectionAgent
Target
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Applying Biodetection Agent to Paper
υ To fibers before papermaking processυ Coating υ Printing on paper
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Approach 1 Approach 1 –– Simply Print a SolutionSimply Print a SolutionCase 1 – no adhesion to paper surface
Example: DNA aptamers on celluloseExposure to target or developing solutions causes biodetection polymer to come off paper Su, et al. Adsorption and Covalent Coupling of ATP-Binding DNA Aptamers onto Cellulose. Langmuir 2007, 23, (3), 1300-1302
Case 2 – very strong adhesion with surface
Example: any anionic protein on wet strength resin coated paperEnzymes and antibodies may denature on surface
Paper
Paper
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Approach 2 Approach 2 –– Cellulose Binding Cellulose Binding DomainsDomains
Cellulase – family of enzymes which degrade celluloseTwo parts – binding domain, catalytic domain (CBD)Sentinel researchers have genetically engineered CBD onto antibodies and phageGives spontaneous binding to pure cellulose with controlled orientationWhat about surfaces coated with size, wet strength resin, retention aids, and/or fillers?
catalyticdomain
bindingdomain
Paper
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Approach 3 Approach 3 –– Carrier ParticlesCarrier ParticlesAttach biodetection molecules to carrier particles which can be formulated into inkThe particles isolate the detectors from the paper surface chemistryThree Sentinel initiatives:– Porous silica particle
Brook/Brennan– Microcapsules Rochefort/Paice– Microgels Pelton/Filipe/Li/Hall
Paper
CarrierParticle
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Microgel Carrier Particles
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Microgel Preparation
• Essentially a surfactant-free emulsion polymerization
• Particles form because polymer is insoluble at high temperature
• Electrostatically stabilized – sulfate from KPS.• Surfactant will give smaller particles
PNIPAM microgel
NH
CH 2
O
NH
O
ONH
CH3C H3
BA NIPAM
Water, 70 C
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All Microgel Properties Depend Upon T
Below 33oC• Microgels• about 90% water• transparent
Above 33oC• latex particle• about 20% water• white
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T.E.M.
υ Pelton and Chibante, Colloids and Surfaces 20, 247 (1986)
~500nm
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Microgels for Bioconjugation
υ Require functional groupsυ We chose carboxyl groupsυ The pioneers:
– Kawaguchi (1992) - first microgel protein interactions– Pichot & Elaissari - microgel with grafted oligo-DNA
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EDCStreptavidin
Biotin-Aptamer
Biotin-
IgG
SP-MG COOH
COOH
COOH
COOH
COOH
COOHAPT-MG COOHCOOH
COOH
IgG-MG
COOHCOOH
COOH
COOH
MG
• IgG or Aptamer still functions in solution after coupled onto microgel surface.
Microgel Derivatization
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Microgel Swelling
200
300
400
500
600
700
2 4 6 8 10pH
Hyd
rody
nam
ic D
iam
eter
(nm
)
MG
SP-MGAPT-MG
IgG-MG
RB-MG
υ ~ 0.2 mg/m2 streptavidin on microgel surface
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Electrophoretic Mobility
MG
SP-MG
APT-MG
IgG-MGRB-MG
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DNA Aptamer Detecting ATP
υ Synthetic short DNA chains which fold to capture a specific target
ATP added
QDNA added
GTP added
ATP-MG in Binding Buffer
+ATP
ATP
Nutiu R., Li Y., J. Am. Chem. Soc., 2003, 125, 4771-4778
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Schemes of the Set-up for Detection
Microgel
Sample solution
Microgel
Sample to be detected
Elution
Scheme 1 Scheme 2
Filter paper
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Blank Filter paper
Filter paper with MG
Before Elution After Elution
“Spotted” Microgels on Filter Paper
• Microgel penetrates into filter paper about 1/3 of the thickness.
• Microgels do not move with elution
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Inkjet Microgel Printing
υ Dimatix Fujifilm Printer
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Printing IgG-MG or APT-MG onto Filter Paper Surface
Ag-Per
IgG-MG
Ag-PerIgG-MGPer
IgG-MG-control
ATP GTP
Quenched APT-MG
• IgG or Aptamer coupled on microgel can survive the printing process.
• Promising for making microgel-based bioinks. Biomacromolecules 2008, 9, (3), 935-941.
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Cationic Paper
υ DNA aptamer is denatured when directly applied to cationic paper.
υ Microgel supported aptamer functions on cationic paper.
Aptamer Directly Applied to PAE Treated Paper
APT-MG on PAE Treated Paper
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Summary
υ Microgels are immobilized on filter paperυ Microgel supported antibodies and DNA aptamers
retain activity on filter paperυ Microgel protects aptamer from cationic polymer
impregnated wet strength paper
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That’s All
Bioactivepaper.comPapersci.mcmaster.ca
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Microgel Superstar
υ B.Eng, Queensυ Ph.D. McMaster 2006υ MIT PDF 2006-2008υ New Faculty at McMaster July 2008
Todd Hoare
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Microgel Microstructure Models
VAA-NIPAM(Surface Model)
-
---
-
-
--
--
--
-
-
--
-
-
-
--
--
--
-
- -
-- -
- ---
----- -
--
-
----
--
MAA-NIPAM(Inverse Core-Shell
Model)
-
-- -
-
- - - -
-
--- --
---
-- -
-- --
---
--- -
-
-
-
- -
--
-
-
-
-
- - - -
rNIPAM = 16.7 rVAA = 0.002 ≈ 0
r/ro0 1
VAA-NIPAM
r/ro0 1
AA -NIPAM
r/ro0 1
MAA-NIPAM
-CO
OH
Con
cent
ratio
n
-- -
-- - - - - - - --
---
---
-- -
--
-
-
-
-
-- - - ----
----
-
- -
--
-
-
---
-
-CO
OH
Con
cent
ratio
n
-CO
OH
Con
cent
ratio
n
AA-NIPAM(Core-Shell Model)
rNIPAM = 0.20±0.08rMAA = 2.8±0.4
rNIPAM = 0.57±0.07
rAA = 0.32±0.04
Direct correlation between monomer reaction kinetics and resulting COOH distributions
Hoare, T.; McLean, D., J. Phys. Chem. B 2006, 110, (41), 20327-20336.
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Chain Transfer with Vinylacetic Acid
• Slow propagation kinetics and chain transfer ability of VAA expect chain end, surface-localization of functional groups
A “one-step” method of making functionalized “hairy” microgels?
R + CH2 CH
CH2
COOH
propagation
CH2
CHCH2
COOH
R
chain transfer
CH2 CH
CHCOOH
CH
CHCOOH
CH2
+ R(terminated)
effective chain transfer(reinitiation)
degradative chaintransfer (H-transfer)
end-capping (termination)
M+CH
CHCOOH
CH2
Mpropagation
+ R
CH
CHCOOH
CH2
RCH2 C
H
CH2
COOH
resonance
propagation
Dominant in allylic radical systems
.
.
..
.
.
Propagation Chain TransferNIPAM
FunctionalMonomer
NIPAMFunctionalMonomer
Hoare, T.; Pelton, R., Macromolecules 2004, 37, (7), 2544-2550.
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Detection of Specific Target by APT-MG (Scheme 2)
0.5 mM GTP
0.5 mM ATP
2 mM GTP
2mM ATP
APT-MG
• APT-MG was mixed with the quencher before applying.
• Paper strips were eluted in ATP or GTP solution.
• Elution in ATP, specific target, enhanced the fluorescence intensity.
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PAE Structure and Reactionsυ Cationic, reactive
water soluble polymerυ Reacts with carboxyl
and amine groupsυ Requires heat to drive
reactionsυ Produces positively
charged paper
Obokata, T.; Isogai, A. Colloids and Surfaces 2007, 302, (1-3), 525-531.
PAEPAE
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Microgel Swelling
υ gel swelling decreases at LCST.
υ ~ 10 timesdecrease in volume
υ Swelling sensitive to ionic strength
0.1M
0.01M
0.001M
CaCl2
ColloidallyUnstable
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DNA Aptamer with Built-in Reporting υ Structure-switching signaling
FŠTCACTGACCTGGGGGAGTATTGCGGAGGAAGGT5’
Q-GTGACTGGACCC 5’
FDNA
QDNA
Target
Non-targetNutiu R., Li Y., J. Am. Chem. Soc., 2003, 125, 4771-4778
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FITC-BSA
Fluorescein-DNA
MGMG
Protein or DNA oligos will Pass Through the Microgel on Paper Surface
MG
FTIC-BSA
• Proteins have molecular level contact with microgel
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Detection of Antigen by IgG-MG (Scheme 2)
Developed in Ag-Per
IgG-MG
IgG-MGDeveloped in Per Developed in
Ag-Per
IgG-MG-control
A: (1.6 µg/ml Ag-Per or Per)
B: (0.16 µg/ml Ag-Per or Per)
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Detection of Antigen by IgG-MG (Scheme 1)
Ag-Per
IgG-MG
Ag-Per
IgG-MGPer
IgG-MG-control
• Antigen was conjugated with peroxidase.
• Further elution in OPD, substrate for peroxidase, gave out color signals.
• Only the strip with antigen showed brown color